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Citations for Stemcell Technologies :
551 - 600 of 4196 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Bulk T cells were isolated from PBMCs using Easysep Human T Cell Isolation Kit (STEMCELL Technologies) and activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were enriched using an EasySep magnetic negative selection kit (Stem Cell Technologies #19853) as per the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... All CD19+ B cells were isolated (STEMCELL Technologies EasySep Mouse B Cell Isolation kit, STEMCELL Technologies) and mRNA was extracted and purified (PureLink RNA Mini Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... RBC-lysed 50,000 whole bone marrow cells were seeded in 1.5mL MethoCult M3434 (Stem Cell Technologies) with no additional supplemental cytokines in triplicate on 6 well plates and scored on day 8 ...
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bioRxiv - Bioengineering 2020Quote: ... at which point cells were split into new wells using ReLeSR passaging reagent (Stem Cell Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then treated with 2ml of 0.25% Trypsin/EDTA solution (Stem Cell Technologies, cat #07901) and 2ml of 5mg/mL dispase (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: Unencapsulated 50 cell aggregates were created by dissociation of human iPSCs with Accutase (STEM CELL Technologies) and reseeding in mTeSR™-1 medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... Primary NK cells were separated from donor PBMCs via magnetic bead negative selection (Stem Cell technologies). 96well ELISA plates (Nunc Maxisorp ...
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bioRxiv - Immunology 2021Quote: ... PE positive cells were enriched using an EasyEights™ magnet (Stem Cell Technologies, Cat no. 18103). Three rounds of separation were performed in total and cells were washed in complete media prior to maintenance in culture with complete RPMI + IL-2 ...
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bioRxiv - Immunology 2021Quote: ... EasySep Mouse Naive CD4+ T cell and CD8+ T cell Isolation Kit (StemCell Technologies, Vancouer, Canada) were purchased.
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bioRxiv - Cell Biology 2020Quote: ... Single cell suspensions were enriched for NK lineage cells using a bivalent antibody RosetteSep (StemCell Technologies)-based method (55) ...
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bioRxiv - Cell Biology 2021Quote: ... CD34+ cells (1 × 105/mL) were suspended in serum-free expansion medium II (Stem Cell Technologies) with 50 ng/mL stem cell factor (PeproTech ...
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bioRxiv - Developmental Biology 2021Quote: ... feeder-free maintenance medium (mTeSR for human ES and iPS cells, Stem Cell Technologies, cat#85850) as previously described (Low et al. ...
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bioRxiv - Neuroscience 2021Quote: ... hiPSCs were detached from the Vitronectin XF substrate using Gentle Cell Dissociation Reagent (Stem Cell Technologies), centrifuged ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using ImmunoCult™ Human CD3/CD28 T Cell Activator antibodies (StemCell Technologies).
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bioRxiv - Immunology 2021Quote: ... CD3+ T cells or CD4+ T cells were isolated via Lymphoprep and RosetteSep (both STEMCELL Technologies). For Treg isolation ...
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bioRxiv - Immunology 2020Quote: ... T-cells were obtained from PBMCs using the EasySep Human T-cell Isolation Kit (Stemcell Technologies). MLRs were set up in 96 well plates ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated with EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies, 17952).
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bioRxiv - Immunology 2021Quote: ... cells were washed in PBS and incubated in PBS + 50 nM dasatinib (Stem Cell Technologies, Canada) + DNAse at 37°C for 30 min ...
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bioRxiv - Genomics 2021Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and T cells isolated using the EasySep Human T Cell Isolation kit (Stemcell Technologies, #17951). Primary human T cells were cultured for up to 20 days in ATCC-modified RPMI (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells from healthy donors were purified from leukopaks purchased from Stem Cell Technologies using Ficoll-Paque PLUS (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: CD3ε+ T cells were isolated using negative selection Human T cell Isolation Kit (StemCell Technology, #17951). Cells were plated at 1 × 106 cells/ml in T cell growth media (StemCell Technology ...
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bioRxiv - Developmental Biology 2022Quote: ... single cell suspensions were prepared by treatment of hES cells with accutase (#07920, StemCell Technologies Inc.) and 1 × 106 cells were seeded per well of an Aggrewell™800 plate in APEL2 medium supplemented with 10 μM Y-27632 ROCK inhibitor (#72307 ...
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bioRxiv - Immunology 2022Quote: ... NK cells isolated from buffy coats using an EasySep human NK cell enrichment kit (StemCell Technologies) were added to the mixed target population at a 1:1 effector:target ratio ...
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bioRxiv - Physiology 2023Quote: ... Cells were washed with 1x PBS and plated in methocullulose media (Methocult M3434, Stem Cell Technologies). Cells were plated (4 × 105 cells per 35 mm-tissue culture dish ...
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bioRxiv - Immunology 2022Quote: ... NK cells were enriched from PBMCs by EasySep™ Human NK Cell Isolation Kit (StemCell Technologies) and cultured at 37 °C in 5% CO2 in RPMI 1640 (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... at which point cells were split into new wells using ReLeSR passaging reagent (Stem Cell Technologies). Accutase (Innovative Cell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were purified by negative selection using RosetteSepTM Human T cell enrichment cocktail (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The CD34+ cells were purified from PBMNCs by the magnetic separation method (EasySep, Stem Cell Technologies) and were cryopreserved ...
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bioRxiv - Neuroscience 2023Quote: ... Single clones were generated by seeding single cells in presence of CloneR (05888, Stem Cell Tech). All clones and the bulk population were routinely subjected to mycoplasma testing ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell clusters were released from Matrigel by incubation in dispase 5U/mL (Stem Cell Technologies 07913) for up to 30 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... human CD4+ T cells were purified from frozen peripheral blood mono-nuclear cells (PBMCs) (STEMCELL Technologies) with EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies) ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... HUDEP-2 cells were expanded in a basal medium composed of StemSpan SFEM (Stem Cell Technologies) supplemented with 10−6 M dexamethasone (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... The cells were then transduced with barcode lentivirus library (31) in StemSpan medium (Stem Cell Technologies) by centrifugation at 900 ×g for 1.5h at room temp ...
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bioRxiv - Cancer Biology 2023Quote: B cell isolation was performed using the the EasySep Human B Cell Isolation Kit (StemCell Technologies). PBMC were diluted in PBS supplemented with 2 % FBS and 1 mM EDTA to a final concentration of 107 cells/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated cells were then reaggregated in low adhesion microwell culture plates (AggreWell-800, Stem cell technologies). 3 million cells were plated per 2mL well in mTesR plus Y-27632 (1 mM ...
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bioRxiv - Neuroscience 2023Quote: ... Medium was changed every day and cells were passaged using gentle cell dissociation reagent (Stemcell Technologies). Differentiation of hiPSCs to glutamatergic neurons was based on a modified method of Chambers et al ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged every 3-5 days after dissociation with ReLeSR(Stem Cell Technologies, 100-0483). The medium was supplemented with Rho-associated protein kinase (ROCK ...
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bioRxiv - Immunology 2023Quote: ... B cells were then isolated using a human B cells enrichment kit (Stemcell Technologies, Cat#19054). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... Primary T cells were isolated from PBMCs using EasySep Human T Cells Isolation Kits (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Neutrophil and monocyte cell population were each obtained by negative selection (>97% purity, Stem Cell Technologies). To generate human monocyte-derived macrophages ...
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bioRxiv - Neuroscience 2023Quote: ... iPSC cell maintenance involves daily media changes in mTeSR Plus (Stem Cell Technologies, cat#100-0276) and a humidified incubator (5% CO2 ...
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bioRxiv - Physiology 2023Quote: ... B cells were isolated via negative selection with an EasySep B cell isolation kit (STEMCELL Technologies)
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bioRxiv - Cell Biology 2023Quote: ... after which ALI was induced and cells maintained in PneumaCult™-ALI media (Stem Cell Technologies).
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bioRxiv - Developmental Biology 2023Quote: ... Cells were treated overnight with 10 µM Rock inhibitor Y-27632 (Cat. # 72304, Stem Cell Technologies) to attenuate cell death ...
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bioRxiv - Developmental Biology 2023Quote: Human mesoderm cells were derived from hESCs using the STEMdiff mesoderm induction medium (Stem Cell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the EBs were separated from loose cells using a reversible strainer (Stem Cell Technologies Cat# 27259) and transferred to a new ultralow attachment plate with EB media (Stem Cell Technologies Cat# 05893) ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were isolated using the EasySep Mouse CD8+ T cell isolation kit (StemCell Technologies) and resuspended at a concentration of 106 cells/mL in complete RPMI supplemented with 1X sodium pyruvate (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... T cells were isolated from PMBCs using a Nonhuman Primate T-Cell Isolation Kit (STEMCELL Technologies), activated with a nonhuman primate T-Cell Activation/Expansion Kit (Miltenyi Biotec) ...