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Citations for Stemcell Technologies :
101 - 150 of 155 citations for Clostridium Difficile Toxin B Ribotype 027 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... B cells were isolated from single cell suspensions by using negative selection kit (Stem Cell Technologies) prior to flow cytometry and cell sorting.
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bioRxiv - Bioengineering 2021Quote: ... The B cells were isolated from fresh blood samples using negative selection kits from Stemcell Technologies (EasySep Direct Human B Cell Isolation Kit) ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified using negative magnetic isolation kit (Miltenyi Biotec Inc. or Stem Cell technologies) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Using EasySep mouse B cell enrichment kit and manufacturer’s protocol (StemCell Technologies Inc., Tukwila, WA, USA), B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... B cells were isolated from PBMCs using a negative selection magnetic separation kit (19054, StemCell Technologies) with at least 70% purity.
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bioRxiv - Cancer Biology 2020Quote: ... Immuno-magnetic T and B cell depletion was performed using the EasySep magnetic system (StemCell Technologies), prior to setting up in vitro cultures ...
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bioRxiv - Cancer Biology 2024Quote: ... in EpiCult™-B (mouse) medium supplemented with human recombinant EGF (hrEGF) (Stem Cell Technologies, 78006), human recombinant bFGF (hrbFGF ...
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bioRxiv - Immunology 2019Quote: ... hMT and s-hMT mice were purified with the EasySep Mouse B Cell Isolation Kit (Stemcell Technologies). B cells were cultured for 2 to 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
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bioRxiv - Cell Biology 2020Quote: ... freshly isolated mammary epithelial cells or transduced cells were cultured in complete Epicult-B medium (STEMCELL Technologies) containing 5 % matrigel (Corning) ...
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bioRxiv - Immunology 2020Quote: ... Plasma samples were diluted by half with PBS during RosetteSep human B cell processing (StemCell Technologies #15064), aliquoted ...
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bioRxiv - Bioengineering 2023Quote: ... the media was fully replaced with 2 mL Hematopoietic Media B (STEMdiff Hematopoietic Basal Media (StemCell Technologies) + STEMdiff Hematopoietic Supplement B (200x ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were cultured for 7 d at 5% O2 in EpiCult- B mouse medium (STEMCELL Technologies, 05610) supplemented with 5% FBS ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells and CD19+ B cells were negatively isolated using magnetic bead based separation (EasySep, StemCell Technologies). CD14+ monocytes were positively selected (EasySep ...
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bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...
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bioRxiv - Immunology 2020Quote: ... The mouse B cell isolation kit and mouse naïve CD4+ T cell isolation kit were from STEMCELL technologies. The purified rat anti-mouse α4β7 (DATK32 ...
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bioRxiv - Immunology 2023Quote: ... B cells were purified from TMNCs with the EasySep Human CD19 Positive Selection Kit II (Stem Cell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was used at 2 µg/mL.11,14 Immunocult-ACF Human B Cell Expansion Supplement (BAC, Stem Cell Technologies) was used at 1:50 dilution per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... Splenocytes were enriched in CD19+ cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) yielding a purity above 95% of CD5+ CD19+ cells ...
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bioRxiv - Cell Biology 2020Quote: ... medium was replaced with STEMdiff™ Hematopoietic Supplement B medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). Cells remained in this medium for 7 additional days ...
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bioRxiv - Cell Biology 2023Quote: FACS purified single cell suspensions (500 cells/per well) were seeded in Human EpiCult-B media (Stem Cell Technologies) supplemented with 5 % FBS on day 0 ...
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bioRxiv - Cancer Biology 2020Quote: ... Isolated Lin− breast cancer cells were cultured in Human EpiCult™-B Medium Kit medium (Stem Cell Technologies, Vancouver, Canada) containing a supplement mix ...
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bioRxiv - Immunology 2022Quote: ... Het and HO mice using EasySep B cell kits by negative selection according to the manufacturer’s instructions (Stemcell Technologies, Canada). B cells were stained with anti CD21-FITC and anti CD23-PE antibodies (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... Primary CD19+ B-cells were isolated from human PBMC using the EasySep human CD19 positive selection kit II (StemCell Technologies) and transduced using LV.B6L as described elsewhere (35) ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and isolated using a negative isolation kit (EasySep™ Mouse B Cell Isolation Kit, #19854, StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood B cells were isolated via negative selection using RosetteSep immunodensity-based cell separation (Stemcell Technologies, Vancouver, BC, Canada). The purity of CLL/ B cells was analyzed by flow cytometry and revealed that ≥ 90% cells co-expressed CD5/CD19.
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bioRxiv - Cancer Biology 2019Quote: ... Untreated peripheral blood B cells were isolated from the PBMCs of healthy donors by negative selection with the RosetteSep for isolation of B cells (StemCell Technologies). All samples were used when they contained ≥95% CD19+ cells ...
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bioRxiv - Microbiology 2020Quote: ... PEG solution was aspirated and cells resuspended initially in ClonaCell-HY Medium B thereafter in ClonaCell-HY Medium C (StemCell technologies) and incubated overnight (37°C ...
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bioRxiv - Microbiology 2020Quote: ... Splenocytes were isolated by shredding the spleen through a mesh followed by washing three times in ClonaCell-HY Medium B (StemCell technologies). Splenocytes were fused to SP2/0 cells using the ClonaCell-HY hybridoma Kit (StemCell cat ...
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bioRxiv - Microbiology 2020Quote: ... purified human B cells were obtained from the Human Immunology Core of the University of Pennsylvania under an Institutional Review Board-approved protocol and were isolated using the RosetteSep Human B Cell Enrichment Cocktail (StemCell Technologies) as per protocol ...
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bioRxiv - Immunology 2022Quote: ... and M2A-GFP KI mice (gift of R. Adelstein, NHLBI/NIH) of either sex using negative selection B cell isolation (StemCell Technologies). Euthanasia was performed in accordance with protocols approved by the National Human Genome Research Institute Animal Use and Care Committee at the National Institutes of Health ...
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bioRxiv - Molecular Biology 2022Quote: ... ESCs were plated on laminin-coated 6 well plates at a density of 65000 cells per well in N2B27 media (as above but with N2 Supplement-B from StemCell Technologies) supplemented with bFGF (10ng/uL bFGF (R&D) ...
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bioRxiv - Immunology 2020Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymoh nodes using CD19 Microbeads (Miltenyi) or Mouse B cell Isolation Kit (Stemcell Technologies). B cells were stimulated with LPS (10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... Then all cell clumps formed in Stemdiff B were transferred to a 24-well transwell (0.4uM pore, Stem cell technologies, #38024) for further differentiation ...
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bioRxiv - Immunology 2023Quote: ... B cell purification was performed according to manufacturer instructions with the Stem Cell EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies). After enrichment ...
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bioRxiv - Immunology 2023Quote: Small resting B cells were isolated from spleen with a discontinuous Percoll gradient and subsequent purification using EasySep Mouse B cell Isolation Kit (StemCell Technologies). B cells were stimulated with 3mM CpG (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... Spleens were macerated through a 70µm mesh filter and cells were separated using the EasySep Mouse B-cell separation kit (StemCell Technologies 19854A). Cells were then treated in B-cell culture media made using 438 mL RPMI 1640 (Gibco 11835-030) ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Both hiPSCs and ESCs showed pluripotency markers and normal karyotype throughout the study (Figure S1A-B) and were grown on matrigel-coated plates in mTeSR medium (Stem Cell Technology) containing the Rock inhibitor ...
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Primary human B cells were isolated from fresh leuko-reduction collar blood by using 750 µL of RosetteSep™ Human B Cell Enrichment Cocktail (Stemcell Technologies) for 10 mL of collar blood ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were first differentiated into hematopoietic progenitor cells using a combined strategy of incorporating primitive hematopoiesis via a WNT switch(77)and sorting and replating CD235a+ cells in Medium B from a commercially available kit (StemCell Technologies #05310). Briefly ...
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bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
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bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156 ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated from WT mice by magnetic bead negative selection using EasySep Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2019Quote: ... Differentiation was initiated by changing to RPMI1640 medium containing B-27 (minus insulin) and the glycogen synthase kinase-3 (GSK3) inhibitor CHIR99021 (STEMCELL Technologies, cat. 72052) for 24 h ...