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Citations for Stemcell Technologies :
51 - 100 of 155 citations for Clostridium Difficile Toxin B Ribotype 027 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... total B cells from the spleens and lymph nodes were isolated using the Negative Selection B cell isolation kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: The coating solution in the top channel was removed and replaced with so-called B/D complete medium: a 1:1 mixture of BEpiCM-b and DMEM medium (STEMCELL Technologies), supplemented with Bronchial Epithelial Cell Growth Supplement (ScienCell) ...
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bioRxiv - Immunology 2020Quote: ... Splenocytes and lung cells were enriched for total B cells using the EasySep Mouse Pan-B Cell Isolation kit (Stemcell Technologies) while whole mln cells were used for downstream processing ...
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bioRxiv - Genomics 2021Quote: ... B cells were purified from thawed PBMCs by negative selection using EasySep Human B Cell Enrichment Kit (Cat#19844, STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Naive B cells were isolated by negative magnetic activated cell sorting using the EasySepTM Mouse B cell Isolation Kit (StemCell Technologies). 30 million B cells were injected i.p ...
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bioRxiv - Microbiology 2024Quote: ... Naïve B cells were then isolated from adult blood using the EasySep Human Memory B Cell Isolation kit (STEMCELL Technologies #17864) to first remove CD27 positive memory cells ...
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bioRxiv - Immunology 2024Quote: B cells were isolated from blood of convalescent COVID-19 donors using the EasySep B kit (Stemcell Technologies, cat no 17954). SARS-nCoV-2 Spike RBD (Raybiotech ...
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bioRxiv - Microbiology 2021Quote: ... cells were centrifuged at 500 x g for 15 min at room temperature and were resuspended in RoboSep buffer for B cells isolation by negative selection with EasySep™ Human B Cell Isolation Kit (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were then enriched for B cells by using the EasySep Human Pan-B cell Enrichment Kit (Stemcell Technologies, Vancouver, BC, Canada). PBMCs were incubated with an antibody cocktail containing CD2 ...
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bioRxiv - Immunology 2020Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Immunology 2019Quote: ... The sample was resuspended in growth media and B cells were negatively selected using the EasySep Human B cell Isolation Kit according to the manufacturer’s protocol (STEMCELL Technologies, Vancouver, BC). Because our samples contained greater than one million PBMCs ...
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bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
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bioRxiv - Microbiology 2019Quote: ... or RosetteSep Human B cell enrichment cocktail (STEMCELL Technologies) and then isolated with Ficoll-Paque Plus (GE Healthcare) ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary cells were resuspended in Epicult-B (Stemcell Technologies) media containing 2% Matrigel and 12,000 cells were seeded into each chamber in a total volume of 400 μL ...
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bioRxiv - Cancer Biology 2021Quote: ... and immersed in EpiCult-B Medium (STEMCELL Technologies, 05610) supplemented with 10 ng/mL human recombinant EGF (PeproTech ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated by negative magnetic selection from either B*08:01+ or B*35:01+ donor blood using kits from StemCell Technologies (catalog #17953). To screen for antigen-specific CTLs ...
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bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
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bioRxiv - Bioengineering 2021Quote: ... Cells were pelleted by centrifugation at 300 g for 10 min and resuspended at 108 cells/mL in EasySep™ buffer and B lineage cells were isolated using an EasySep™ mouse B cell negative isolation kit (StemCell Technologies, #19854). For serum ...
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bioRxiv - Immunology 2021Quote: ... Cells from all mice were pooled by tissue type and B cells were selected from lymph node and spleen using a mouse Pan-B negative selection kit (Stemcell Technologies, Vancouver, BC, Canada). Generation of natively paired heavy and light chain libraries ...
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bioRxiv - Immunology 2020Quote: ... B cells were enriched by negative selection using EasySep mouse B cell enrichment kit as per manufacturer’s instructions (StemCell Technologies Inc., Tukwila, WA, USA). Enriched B cells were then resuspended in FACS buffer and stained and sorted as described previously(Carbonetti et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... malignant B cells were enriched from splenocytes of Eµ-TCL1 mice using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, Inc., Cologne, Germany). The CD5+ CD19+ content of purified cells was typically above 95% ...
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bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were replated in prepared Medium B from Stemcell technologies. The manufacturer’s protocol was followed for an additional 7-10 days and numerous CD43+ progenitor cells (HPC ...
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bioRxiv - Microbiology 2020Quote: ... Primary human B cells were isolated by negative selection using RosetteSep Human B Cell Enrichment and EasySep Human B cell enrichment kits (Stem Cell Technologies, #15064 and #19054), according to the manufacturers’ protocols ...
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bioRxiv - Physiology 2020Quote: ... enteroids were maintained in Human IntestiCult components A+B (STEMCELL Technologies). To promote differentiation ...
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bioRxiv - Microbiology 2024Quote: ... the Easy Human Pan-B Cell Enrichment kit (STEMCELL Technologies #19554) was used ...
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bioRxiv - Immunology 2021Quote: ... after B cell enrichment with the negative selection cocktail (Stem Cell Technologies), cells were labeled with anti-CD19-PEcy7 ...
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bioRxiv - Immunology 2022Quote: ... The blood was treated with 2.5 μl/ml Rosettesep B (Stemcell technologies) for 20 mins at room temperature.
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bioRxiv - Immunology 2020Quote: ... using the EasySep Human Naive B Cell Enrichment Kit (19254; STEMCELL Technologies), from healthy donor PBMCs ...
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bioRxiv - Immunology 2023Quote: ... TMNCs or purified B cells were frozen in Cryostor (Stem Cell Technologies) and stored in liquid nitrogen until use.
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bioRxiv - Immunology 2023Quote: Frozen human peripheral blood CD19+ B cells were purchased from StemCell Technologies and thawed per manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... of either sex using a B-cell isolation kit (Stemcell Technologies; catalogue #19854) to deplete non-B cells ...
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bioRxiv - Neuroscience 2021Quote: ... medium was replaced with 2 mL/well iHSC Medium B (StemCell Technologies #05310). An additional 1 mL/well of iHSC Medium B was added every other day ...
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bioRxiv - Cancer Biology 2022Quote: ... the whole BM samples were incubated with Human B RosetteSep (Stemcell Technologies, CAN) following the manufacturer ‘instructions.
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bioRxiv - Cancer Biology 2019Quote: ... in complete Epicult-B Medium supplemented with 0.48 µg/mL hydrocortisone (Stemcell technologies) overnight at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: Primary mammary epithelial cells were cultured in EpiCult-B mouse medium (STEMCELL Technologies) supplemented with proliferation supplements ...
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bioRxiv - Immunology 2022Quote: ... and naïve B cells were isolated by negative selection (StemCell Technology, Catalog #19254). B cells were seeded in 24 well flat-bottom plates at a density of 1 × 105 per well and stimulated in 1 ml of B cells complete medium ...
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bioRxiv - Immunology 2022Quote: ... Splenic B cells were enriched with the EasySep kit (STEMCELL Technologies, Cambridge, MA). Bone marrow cells were obtained by flushing femora and tibiae with RPMI medium ...
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bioRxiv - Immunology 2022Quote: ... for 3 days and Stemdiff B (STEMdiff™ Hematopoietic Kit, Stem cell technologies) for 7 days as described previously (41) ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: Frozen human peripheral blood CD19+ B cells were purchased from StemCell Technologies (Vancouver, Canada). Cells were thawed per manufacturer’s instructions and cultured as previously described.60 Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.25 μg/mL amphotericin B) supplemented with 10 μM Y-27632 (STEMCELL Technologies). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... Isolation of T and B cells were performed according to the manufacturer’s protocols (Stemcell Technologies).
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bioRxiv - Pathology 2019Quote: ... iPSCs were exposed to N2 media containing 5 mL N2 Supplement-B (Stemcell Technologies 07156), 0.5mL 55mM β-mercaptoethanol (Life technologies 21985-023) ...
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bioRxiv - Bioengineering 2021Quote: ... the medium was replaced with RPMI1640 enriched with 5ng/mL PDGF-B (Stem Cell Technologies), 2.5 ng/mL TGF-B (PeproTech) ...
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bioRxiv - Immunology 2022Quote: ... and human B-cell activating factor (BAFF) in 80 mL StemCell medium A (StemCell Technologies) containing 1 μg/mL of cyclosporine A (Millipore-Sigma) ...
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bioRxiv - Genomics 2020Quote: ... B lymphocytes and CD4+ T lymphocytes were isolated from PBMCs by negative selection (STEMCELL Technologies; cat ...
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bioRxiv - Immunology 2021Quote: ... and magnetic beads were used for negative selection of bulk B cells (StemCell Technologies, 19054) or naïve B cells (17254) ...