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Citations for Stemcell Technologies :
401 - 450 of 649 citations for 6 TERT BUTYL 1H PYRAZOLO 3 4 B PYRIDIN 3 AMINE 95% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Het and HO mice using EasySep B cell kits by negative selection according to the manufacturer’s instructions (Stemcell Technologies, Canada). B cells were stained with anti CD21-FITC and anti CD23-PE antibodies (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... Primary CD19+ B-cells were isolated from human PBMC using the EasySep human CD19 positive selection kit II (StemCell Technologies) and transduced using LV.B6L as described elsewhere (35) ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and isolated using a negative isolation kit (EasySep™ Mouse B Cell Isolation Kit, #19854, StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood B cells were isolated via negative selection using RosetteSep immunodensity-based cell separation (Stemcell Technologies, Vancouver, BC, Canada). The purity of CLL/ B cells was analyzed by flow cytometry and revealed that ≥ 90% cells co-expressed CD5/CD19.
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bioRxiv - Biochemistry 2020Quote: ... Cells were seeded at a 1:6 ratio and routinely passaged with Dispase (Stemcell Technologies).
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bioRxiv - Biochemistry 2022Quote: ... hiPSCs were seeded on Matrigel-coated 6-well plates with mTeSR plus medium (STEMCELL Technologies) and 10 μM Y27632 ...
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bioRxiv - Immunology 2021Quote: ... 6 tissue biopsies from each endoscopic region were placed in HypoThermosol medium (Stemcell Technologies 07935) and were transported on ice to the laboratory for further processing.
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bioRxiv - Immunology 2023Quote: ... CD11c+ cells were isolated from the spleens of C57BL/6 by positive selection (Stemcell Technologies) to serve as antigen presenting cells ...
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bioRxiv - Neuroscience 2023Quote: ... 1.5 nM bone morphogenetic protein 4 (BMP4, Stemcell technologies) was added to the medium for another three days ...
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bioRxiv - Cancer Biology 2024Quote: ... transferred into 4 mL methylcellulose (Stem Cell Technologies, 4434) and vortexed vigorously ...
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bioRxiv - Cancer Biology 2019Quote: ... Untreated peripheral blood B cells were isolated from the PBMCs of healthy donors by negative selection with the RosetteSep for isolation of B cells (StemCell Technologies). All samples were used when they contained ≥95% CD19+ cells ...
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bioRxiv - Microbiology 2020Quote: ... PEG solution was aspirated and cells resuspended initially in ClonaCell-HY Medium B thereafter in ClonaCell-HY Medium C (StemCell technologies) and incubated overnight (37°C ...
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bioRxiv - Microbiology 2020Quote: ... Splenocytes were isolated by shredding the spleen through a mesh followed by washing three times in ClonaCell-HY Medium B (StemCell technologies). Splenocytes were fused to SP2/0 cells using the ClonaCell-HY hybridoma Kit (StemCell cat ...
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bioRxiv - Microbiology 2020Quote: ... purified human B cells were obtained from the Human Immunology Core of the University of Pennsylvania under an Institutional Review Board-approved protocol and were isolated using the RosetteSep Human B Cell Enrichment Cocktail (StemCell Technologies) as per protocol ...
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bioRxiv - Immunology 2022Quote: ... and M2A-GFP KI mice (gift of R. Adelstein, NHLBI/NIH) of either sex using negative selection B cell isolation (StemCell Technologies). Euthanasia was performed in accordance with protocols approved by the National Human Genome Research Institute Animal Use and Care Committee at the National Institutes of Health ...
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bioRxiv - Molecular Biology 2022Quote: ... ESCs were plated on laminin-coated 6 well plates at a density of 65000 cells per well in N2B27 media (as above but with N2 Supplement-B from StemCell Technologies) supplemented with bFGF (10ng/uL bFGF (R&D) ...
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bioRxiv - Immunology 2020Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymoh nodes using CD19 Microbeads (Miltenyi) or Mouse B cell Isolation Kit (Stemcell Technologies). B cells were stimulated with LPS (10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... Then all cell clumps formed in Stemdiff B were transferred to a 24-well transwell (0.4uM pore, Stem cell technologies, #38024) for further differentiation ...
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bioRxiv - Immunology 2023Quote: ... B cell purification was performed according to manufacturer instructions with the Stem Cell EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies). After enrichment ...
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bioRxiv - Immunology 2023Quote: Small resting B cells were isolated from spleen with a discontinuous Percoll gradient and subsequent purification using EasySep Mouse B cell Isolation Kit (StemCell Technologies). B cells were stimulated with 3mM CpG (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... Spleens were macerated through a 70µm mesh filter and cells were separated using the EasySep Mouse B-cell separation kit (StemCell Technologies 19854A). Cells were then treated in B-cell culture media made using 438 mL RPMI 1640 (Gibco 11835-030) ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... Donor 2395 iPSC were maintained on Matrigel-coated 6 well plates in mTeSR+ medium (StemCell Technologies) and differentiated to sBC as previously described with some modifications (29) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell colonies were separately dissociated and transferred into 6-well plates using ReLeSR (Stem Cell Technologies). Differentiated cells were removed by using a pipette tip to minimize spontaneous differentiation ...
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bioRxiv - Bioengineering 2019Quote: ... onto 6-well plates coated with 1mL/well of 1µg/cm2 Vitronectin XF (STEMCELL Technologies 07180) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Interleukin-6 (10 ng/mL) and Heparin (5 U/mL) (all supplied by Stem Cell Technologies).
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bioRxiv - Bioengineering 2022Quote: ... coated 6 well plates were used to culture WA09 cells with mTESR plus medium (Stemcell Technologies) in an incubator at 37 degrees Celsius and 5% CO2 ...
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bioRxiv - Genomics 2022Quote: ... rehydrated tissue was placed into a well of a 6-well plate (Stem Cell Technologies #38015) with 1 mL of ice-cold CST lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... responder CD4+ T cells were isolated from OTII BL/6 mice by negative selection (STEMCELL Technologies). Splenocytes from wild-type or HLA-A2+ BL/6 mice were depleted of Thy1.2+ cells (STEMCELL Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... with 6 μM CHIR99021 in 1.2 mL STEMdiff™ APEL™2 medium (STEMCELL Technologies, # 05270) at the bottom of the chamber for 1-hour ...
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bioRxiv - Neuroscience 2023Quote: ... and plated into a single well of a 6-well Aggrewell plate (Stemcell Technologies Aggrewell 800) that was coated with 2 ml of anti-adherence rinsing solution (Stem cell technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Both hiPSCs and ESCs showed pluripotency markers and normal karyotype throughout the study (Figure S1A-B) and were grown on matrigel-coated plates in mTeSR medium (Stem Cell Technology) containing the Rock inhibitor ...
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were first differentiated into hematopoietic progenitor cells using a combined strategy of incorporating primitive hematopoiesis via a WNT switch(77)and sorting and replating CD235a+ cells in Medium B from a commercially available kit (StemCell Technologies #05310). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Primary human B cells were isolated from fresh leuko-reduction collar blood by using 750 µL of RosetteSep™ Human B Cell Enrichment Cocktail (Stemcell Technologies) for 10 mL of collar blood ...
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bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Systems Biology 2020Quote: ... APC-CD49f (4 µL; Stem Cell Technologies 10109, clone VU1D9), Biotin-CD2 (8 µL ...
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bioRxiv - Neuroscience 2022Quote: ... Colonies were passaged every 4 days with ReLeSR (Stemcell technologies) after rinsing with Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Cell Biology 2021Quote: IMR90-4 iPSCs (WiCell) were cultured in mTesR1 (Stemcell Technologies) on Matrigel (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... were passaged 1:4 using ACCUTASE™ (Stemcell technologies, 07920) when confluent and subsequently grown in Astrocyte medium containing leukemia inhibitory factor (LIF ...
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bioRxiv - Neuroscience 2023Quote: ... and passaging every 4-5 days using ReLeSR (StemCell technologies). hES-qualified Matrigel (Corning ...
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bioRxiv - Immunology 2023Quote: ... 4*106 iPSCs were seeded per Aggrewell 800 (Stemcell Technologies) in a 24-well plate in 2 ml embryonic body medium (EBM) ...
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bioRxiv - Cell Biology 2022Quote: ... medium was replenished daily and were passaged every 5-6 days with Dispase I (StemCell Technologies, 07923) in small cell clumps ...
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bioRxiv - Genomics 2021Quote: ... in a well of a Matrigel-coated 6 well plate supplemented with 10μM Y27632 (Stem Cell technologies).
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bioRxiv - Cell Biology 2022Quote: ... and WTC-11 lines and cultured in 6-well plates were dissociated with TRPYLE (Stem Cell Technology), centrifuged at 300 g ...
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bioRxiv - Immunology 2022Quote: ... Splenocytes from wild-type or HLA-A2+ BL/6 mice were depleted of Thy1.2+ cells (STEMCELL Technologies), irradiated (20 Gy ...