Labshake search
Citations for Stemcell Technologies :
601 - 649 of 649 citations for 6 TERT BUTYL 1H PYRAZOLO 3 4 B PYRIDIN 3 AMINE 95% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... tissue was viably frozen by placing 4 biopsies in 500 ml of CryoStor CS10 freezing media (STEMCELL Technologies). Tubes were inverted and then placed on ice for up to 30 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were cultured for 4 days using STEMdiff™ Definitive Endoderm Differentiation Kit (Cat. No. 05110, STEMCELL Technologies) for definitive endoderm induction ...
-
bioRxiv - Developmental Biology 2023Quote: ... iPSCs were cultured for 4 days using STEMdiff™ Definitive Endoderm Differentiation Kit (Cat. No. 05110, STEMCELL Technologies). Media was replaced with RPMI 1640 medium ...
-
bioRxiv - Developmental Biology 2023Quote: ... MEF feeders were inactivated at passage 4 with chemical treatment of 1 μg/ml mitomycin-c (STEMCELL Technologies) overnight ...
-
bioRxiv - Neuroscience 2024Quote: ... hESCs were fed every two days and split every 4–7 days using ReLeSR (StemCell Technologies, 100-0483) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using ReLeSR (Stemcell Technologies, 05872).
-
bioRxiv - Neuroscience 2022Quote: ... Neurons were cultured DIV 0 to DIV 4 in Neurocult™ Neuronal Plating Medium (Stemcell Technologies, catalog no. 05713) with SM1 neuronal supplement (Stemcell Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended in 4 ml Red Blood Cell Lysis buffer (Ammonium Chloride solution, Stemcell Technology, #07800) on ice for 2 min and washed with 6 mL DME/F-12 medium at 450 g for 10 min ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent and cryopreserved in mFreSR according to manufacturer’s instructions (STEMCELL Technologies). All lines were expanded and stored at low passage number.
-
bioRxiv - Cell Biology 2022Quote: ... M2 polarization was achieved by stimulating MΦ cells with 20 ng/mL of interleukin 4 (IL4; Stemcell Technologies, Cat#78045) and interleukin 13 (IL13 ...
-
bioRxiv - Genomics 2021Quote: ... Cells were split every 3 or 4 days with TrypLE Select (Fisher) and seeded at 1.5-4×10e5 in a Matrigel-coated p35 plate containing 5μM Y27632 (Stem Cell Technologies) (mTeSR+Y ...
-
bioRxiv - Cell Biology 2023Quote: ... The medium was replaced on d1 with RPMI/B27™ minus insulin supplemented with 5 µM IWP-4 (STEMCELL Technologies). On d3 medium change was performed using RPMI/B27™ minus insulin without any supplementation ...
-
bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated overnight at 4°C with primary antibody (beta-tubulin III 1:500; STEMCELL Technologies 60052) diluted in blocking buffer ...
-
bioRxiv - Immunology 2023Quote: ... Diluted blood was overlaid carefully on SepMate-15 tubes each containing up to 4 mL Lymphoprep (STEMCELL Technologies, catalog #07851). The layered blood was centrifuged at 1,200 × g for 10 minutes at RT ...
-
bioRxiv - Bioengineering 2023Quote: ... for 4 days to induce iMSCs before the medium was changed to Complete MesenCult™-ACF Plus Medium (STEMCELL Technologies) for an additional 2 days and then the cells were transferred into a new 24-well plate without an MEF feeder layer ...
-
bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
-
bioRxiv - Genomics 2020Quote: ... H1 cells were daily fed with fresh mTeSR1 media and passaged every 4-5 days using ReLeSR reagent (StemCell Technologies, 05872). Cells were dissociated into single cells with TrypLE Express (Thermo Fisher ...
-
bioRxiv - Genomics 2021Quote: ... Cord blood MNCs (4) were received frozen and then CD34+ selected using the EasySep human whole blood CD34 positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... Cryopreserved PBMCs were quickly thawed and resuspended at a concentration of 4×106 cells/ml in Eagle's minimal essential medium (EMEM; Stem Cell Technologies; supplemented with 10% FBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... the cells where count and seeded at a density of 2×10^4 cells per replicate into cytokine-supplemented methylcellulose medium (MethoCult M3434, Stemcell Technologies). After 10-14 days the colonies propagated and scored ...
-
bioRxiv - Developmental Biology 2022Quote: ... they were specified into a hiPSC-CM/stromal cell mixture using RPMI B27-containing 5 µM IWP-4 (Stem Cell Technologies) followed by another 7 days of RPMI B27+ (RPMI1640 GlutaMAX + 2% B27 supplement with insulin ...
-
bioRxiv - Cancer Biology 2021Quote: IL-10 polarized human DCs (3×10^4/well) were incubated with allogeneic CD8+ T cells (1.5 × 10^5/well) in T cell media (Stem Cell Technologies) in 96 well flat bottom tissue culture plates for 4 days ...
-
bioRxiv - Immunology 2021Quote: ... CD34+ umbilical cord and CD34+ hematopoietic stem/progenitor cell (Ethics Committee Salzburg approval 415-E/1776/4-2014) were isolated using density gradient centrifugation (Lymphoprep; Stemcell Technologies). Primary human cells ...
-
bioRxiv - Immunology 2022Quote: HLA-A2+ monocytes were differentiated into moDCs for 7 days with GM-CSF (50 ng/ml) and IL-4 (100 ng/ml; both STEMCELL Technologies); media and cytokines were refreshed every 2–3 days ...
-
bioRxiv - Neuroscience 2023Quote: hiPSC line (IMR90-4, WiCell) derived from a healthy donor and fully characterized was cultured with mTeSRTM Plus Medium (STEMCELL Technologies) on growth factor reduced matrigel-coated 6-well plates (Corning ...
-
bioRxiv - Cell Biology 2023Quote: ... For immunolabelling embryos were washed 4 times with PTwH (PBS/0.2% Triton-X-100/10 µg/mL heparin (Stemcell Technologies, #07980)) for 1 h each at 37°C and incubated with primary antibody (PE-labelled rabbit anti-CD31/PCAM1 (1:100 ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were transferred into TeSR-E7 medium and maintained until the emergence of iPSC colonies (about 4 weeks) which were then manually isolated and propagated in mTeSR medium (StemCell Technologies). Absence of plasmid integration and clearance of Sendai virus were confirmed ...
-
bioRxiv - Neuroscience 2023Quote: ... iPSC cultures were passaged every 4-5 days when the colonies reached 80% confluency using the dissociation reagent ReLeSR (STEMCELL Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed with basal medium (500g, 4°C) and replated in Matrigel in mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies) supplemented with Y-27632 (10 μM) ...
-
bioRxiv - Immunology 2024Quote: ... were differentiated into TH2 T cell subtype by incubation with specific supplement for TH2 differentiation (mouse IL-2, mouse IL-4 and rat anti mouse IFNγ with 1:100 dilution (STEMCELL Technologies)) and incubated for 3 days on plates coated with aCD3 and aCD28 antibodies ...
-
bioRxiv - Cancer Biology 2021Quote: Cells were plated on uncoated low-adhesion 96-well plates in a two-fold dilution series spanning from 2000 down to 4 cells per well in NeuroCult NS-A media (StemCell Technologies, #05751) containing doxycycline at 2 μg/mL ...
-
bioRxiv - Molecular Biology 2022Quote: ... The medium was changed daily and the cells were passaged every 4–8 days depending on confluency using Gentle Cell Dissociation Reagent (Stem Cell Technologies). 10 μM of ROCKi (Stem Cell Technologies ...
-
bioRxiv - Genomics 2019Quote: ... and for 1 h at 4 0C with a combination of conjugated antibodies to surface proteins TRA-1-60 (StemCell Technologies, 60064PE), TRA-1-81(StemCell Technologies ...
-
bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal bovine serum and subsequently resuspended at a concentration of 5×105 cells/mL in 4-5mL of MethoCult H4230 methylcellulose (STEMCELL Technologies, 04230) containing penicillin/streptomycin ...
-
bioRxiv - Cell Biology 2021Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4 °C with a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... 2×10^4 cells from bone marrow of xenotransplanted mice were transferred to 2ml of MethoCult H4034 optimum methylcellulose medium (Stem Cell Technologies) and plated onto two 35mm dishes ...
-
bioRxiv - Cell Biology 2020Quote: ... the imaging chamber was incubated overnight at 4° C with 10 μg/mL of a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) in PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... The differentiation was started following 24 h of seeding and proceeded through a 7-stage differentiation protocol (stages 1 – 4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
-
bioRxiv - Immunology 2021Quote: ... Cells were fed for 4-5 days until airlifted and continued to be grown at air-liquid interface (ALI) with PneumaCult ALI medium (StemCell Technologies, #05001) until fully differentiated (4 weeks)
-
bioRxiv - Cell Biology 2020Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4° C with a mouse monoclonal anti-CD3 antibody (clone UCHT1, StemCell Technologies, #60011) or a mouse monoclonal anti-HA antibody (clone 16B12 ...
-
bioRxiv - Neuroscience 2022Quote: aNSCs/NPCs were cultured as described above (see Primary NSC cell culture) until Passage 2-4 and then passaged with Accutase (STEMCELL Technologies, 07920) and seeded at a density of 5,000 cells per well on Matrigel (Corning ...
-
bioRxiv - Neuroscience 2022Quote: NSCs were cultured as described above (see “Primary NSC cell culture”) in complete activated media until Passage 2-4 and then passaged with Accutase (STEMCELL Technologies, 07920) and seeded at a density of 5,000 cells per well in Matrigel (Corning ...
-
bioRxiv - Cell Biology 2023Quote: ... The hESCs H1 cell-lines were differentiated using the extended 7-stage protocol (stages 1–4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
-
bioRxiv - Genetics 2024Quote: ... cells were harvested by centrifugation at 380 x g for 10 min at 4°C and resuspended in EasySepT Buffer (Stem Cell Technologies). HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) ...
-
bioRxiv - Bioengineering 2021Quote: Testicular organoids were made by mixing germ and somatic cells at a 1:4 ratio and placing them into AggreWell800™ microwells (STEMCELL Technologies, 34811) overnight ...
-
bioRxiv - Bioengineering 2023Quote: ... IMR90-4 iPSCs (WiCell, WB65317) between passages p35∼45 were maintained on Matrigel-coated dishes in mTeSR Plus medium (STEMCELL Technologies, 100-0276) and dissociated using ReLeSR (STEMCELL Technologies ...
-
bioRxiv - Immunology 2023Quote: ... were cultured for 4 days post-split in IntestiCult™ Organoid Growth Medium and then switched IntestiCult™ Organoid Differentiation Medium (STEMCell Technologies) for 72 hours to promote epithelial stem cell differentiation ...
-
bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich], and 10 uM Y27632 [72304, StemCell Technologies] in mTeSR1), and centrifuged at 3200 xg for 30 to 90 min ...
-
bioRxiv - Cell Biology 2020Quote: Flow cytometer-sorted murine or human HSCs were resuspended in 4 mL complete MethoCult GF M3434 medium or MethoCult™ H4435 Enriched (STEMCELL Technologies, 03434 and 04435) thawed at RT and 1 mL of the suspension was placed into 35 mm dishes in triplicates ...