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Citations for Stemcell Technologies :
401 - 450 of 4243 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... via Ficoll-Paque (Cytavia) gradient selection for peripheral blood mono-nuclear cells followed by human CD8+ T cell negative magnetic selection (Stem Cell Technologies). Primary CD8+ T cells were thawed in supplemented RPMI 1640 2-12 hours prior to the start of experiments.
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC-derived DA neurons were plated on a 48-well Classic MEA plate (Axion BioSystems) at a cell density of 1.2×105 cells per well and maintained in culture using Complete BrainPhys™ (Stem Cell Technologies). Culture Medium prepared as directed in the iCell DopaNeurons MEA Application Protocol (https://www.fujifilmcdi.com/) ...
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bioRxiv - Immunology 2023Quote: ... filtered through 40 µm cell strainers and T cells enriched by negative selection using an EasySep™ pan-T cell enrichment kit (Stemcell Technologies) as per manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
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bioRxiv - Cancer Biology 2023Quote: ... the medium containing hematopoietic cells and trypsinized cells were collected for FACS analysis and CFU-C assay in Methylcellulose M3434 (Stem Cell Technologies). After 10 days of culture at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... After an additional 48 hours non-adhered cells were discarded and adhered T-ALL cells were separated from glioblastoma cells using EasySep Human CD3 positive Selection kit II (Stem Cell Technologies) and viability was determined by trypan blue staining (Bio-Rad) ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6low and CCR6high memory subgroups and cultured at 1 × 106 cells/ml in 24 well plates in ImmunoCult XF T cell expansion medium (Stem Cell Technologies) supplemented with 10% FBS and IL-2 (200 units/ml) ...
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bioRxiv - Cell Biology 2023Quote: ... Accutase was quenched and single cells were seeded for differentiation in suspension at a density of 1×106 cells/mL in complete mTeSR media (Stem Cell Technologies) supplemented with 10ng/mL FGF2 (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... apoptotic and dead cells were removed by immunomagnetic cell separation using the Annexin Dead Cell Removal Kit (StemCell Technologies, cat. no. 17899) and EasySep Magnet (StemCell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ HSPCs were then seeded into 96-well round bottom plates (cell density at 100,000 (100k) cells/100 μL) and prestimulated in StemSpanTM SFEM (Stem Cell Technologies #09650) supplemented with StemSpan™ CC100 (Stem Cell Technologies #02690) ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells that passed through 70-uM cell strainer were purified with EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies Inc). For T-cell proliferation assays ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells and isolated CD4+ T cells were activated using Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies, 10970) per manufacturer instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... T cells were then resuspended at 1×106 cells/mL in ImmunoCult™-XF T Cell Expansion Medium (Stemcell Technologies, Cat.#10981) supplemented with Human Recombinant IL-2 (12.5 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... T cells from the transgenic mouse splenocytes were enriched by magnetic isolation using the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies). T cells were centrifuged and resuspended in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were plated for single cell clones by plating 1000 cells per 10cm2 dish on Synthemax with 1x CloneR (Stemcell technologies #05888), and single cell clones were genotyped by PCR for correct integration before sequencing both alleles from positive clones.
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bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
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bioRxiv - Immunology 2022Quote: Preparation of cells: Human CD4+ cells were isolated from buffy coats using EasySep™ Human CD4+ T Cell Isolation Kit (Stemcell Tech, # 17952), resuspended with complete RPMI (cRPMI ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
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bioRxiv - Developmental Biology 2022Quote: ... NPC-iPS cells were treated with 10 µM Rho kinase inhibitor Y-27632 overnight before dissociated to single cells with Accutase (Stem Cell Technology 07922). About 10 million cells were electroporated with 10 μg of AAVS1 zinc finger nuclease-encoding plasmid and 30 μg of AAVS1-tdTomato with one electroporation of 250 V ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
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bioRxiv - Immunology 2021Quote: Purified CD4+CD25− T cells were magnetically isolated from spleen and peripheral lymph node mononuclear cells using an EasySep magnetic T cell negative isolation kit (Stem Cell Technologies, Inc.) with addition of a biotin anti-mouse CD25 antibody (0.5 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated from the pooled cell suspensions using an EasySep(tm) Mouse CD4+ T Cell Isolation Kit from Stemcell Technologies (Vancouver, Canada) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... by plating them at 125.000 cells/cm2 on Matrigel and growing the cells in STEMdiff™ Neuron Differentiation Kit (Stem Cell Technologies, #08500) medium for one week with daily medium changes.
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bioRxiv - Cancer Biology 2024Quote: ... The irradiated negative fraction was passed through a 70uM filter to create a single-cell suspension prior to counting and seeding at 2e6 cells per nanobioreactor in StemPro media (Stem Cell Technologies Inc). The CD34+ fraction was resuspended in 1X StemPro-34 SFM medium supplemented with a cytokine cocktail of SCF (50ng/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... 600K CD34+ Fucci2BL transduced cells were seeded into nanobioreactors pre-cultured with the allogenic CD34- cell fraction and StemPro media (Stem Cell Technologies Inc.).
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated using a mouse B cell isolation kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: Human embryonic stem cells (hESCs) were routinely cultured in mTeSR1 medium (Stem Cell Technologies) on growth factor-reduced Matrigel-coated dishes (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... Surviving cells were expanded and then passaged as single cells using Accutase (STEMCELL Technologies) in MEF-CM supplemented with 10 µM ROCK inhibitor to grow clonal colonies ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were dissociated with 5 ml Accutase™ cell dissociation reagent (STEMCELL technology, 07920). After washing once with PBS ...
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bioRxiv - Cell Biology 2019Quote: ... 100 GFP+ cells from transduced MPP4 cells were plated in Methocult M3630 (StemCell Technologies) supplemented with FMS-like tyrosine kinase like 3 ligand (FLT3L ...
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bioRxiv - Bioengineering 2020Quote: ... Bulk T cells were isolated using RosetteSep Human T cell Enrichment Cocktail (StemCell Technologies). Peripheral mononuclear blood cells (PBMCs ...
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bioRxiv - Neuroscience 2020Quote: ... iPSCs at ~70% confluence were dissociated into single cells with Accutase (Stem Cell Technologies) and 10,000 cells/well were seeded into a 96-well U-Bottom Low-Attachment plate (Corning ...
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bioRxiv - Bioengineering 2020Quote: T-cells were isolated with the EasySep Human T-cell isolation Kit (STEMCELL Technologies) from frozen human peripheral blood mononuclear cells obtained from healthy donors ...
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bioRxiv - Cancer Biology 2021Quote: ... live cells were purified using EasySep Dead Cell Removal (Annexin V) Kit (StemCell Technologies). The remaining viable cells were further incubated in complete media without CRYBMIM for 6-9 days with media replacement every 3 days ...
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bioRxiv - Cancer Biology 2020Quote: ... 25,000 cells were plated in duplicate in MethoCult GF M3434 medium (Stem Cell Technologies) and cultured at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... and then were gently suspended with gentle cell dissociation solution (Stem Cell Technologies Inc.) and incubated for 7 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... or the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The CD34+ cells were cryopreserved at 10e6 cells/mL in Cryostor CS10 (STEMCELL Technologies) according to the manufacturer’s protocol and the remaining hemogenic endothelium was cultured in 2mL of monocyte factory medium ...
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bioRxiv - Cancer Biology 2021Quote: ... and activated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Stem Cell Technologies) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... splenic B cells were purified with a mouse B cell isolation kit (StemCell Technologies), and then stimulated with lipopolysaccharide (LPS ...
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bioRxiv - Developmental Biology 2021Quote: H9 hESCs cells were routinely cultured in mTeSR1 (Stem Cell Technologies; Cat. No. 85850) and growth factor reduced Matrigel (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: Cultured cells were detached from the plates using Accutase cell detachment solution (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: CD4+ T cells were isolated using negative selection (Stem Cell Technologies CD4 enrichment kit) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... 4000 cells were plated per dish for WM cell lines (Methocult H4330 StemCell Technologies). Colonies were scored on day 7 after plating.
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bioRxiv - Genetics 2022Quote: ... cells were placed in HSC base medium (IMDM containing 20% BIT (Stem Cell Technologies), 100ng/mL recombinant human (rh ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were maintained in mTeSR Plus media with supplement (Stem Cell Technologies, Cat.#05825) at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: Human iPSC (XCL-1) derived neural progenitor cells (NPCs, Stem Cell Tech cat#70901) were maintained in serum-free STEMdiff™ Neural Progenitor Medium 2 (Stem Cell Tech cat#08560 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were grown in triplicates at 106/ml in Myelocult H5100 (Stem Cell Technologies) with (20ng/ml ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were passaged at 80-90% confluency using ReLeSR (Stem Cell Technologies Catalog # 05873). Cells were incubated at 37°C in 5%CO2.