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Citations for Stemcell Technologies :
601 - 650 of 4243 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... cell monolayers were maintained for 20-30 days in STEMDiff cardiomyocte maintenance media (STEM CELL Technologies), on EStim classic 48 well MEA plates (Axion Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were cultured in Mammocult media supplemented with 4 μg/ml Heparin (Stem Cell Technology), 0.5 μg/ml hydrocortisone and 10% Mammocult Proliferation Supplement (Stem Cell Technology) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were adjusted to 1×105 cells/ml final concentration in H4230 methylcellulose medium (STEMCELL Technologies) supplemented with 10% 5637-CM as a stimulant and then plated in 35-mm petri dishes in duplicate and allowed to grow for 7 days in a humidified CO2 incubator (5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: Cells were dissociated from the well using Gentle Cell Dissociation reagent (StemCell Technologies, Cat#: 100-0485). Total proteins were extracted by adding 1ml of cold 10% TCA to the cell pellets ...
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bioRxiv - Cell Biology 2021Quote: ... Cell colonies were separately dissociated and transferred into 6-well plates using ReLeSR (Stem Cell Technologies). Differentiated cells were removed by using a pipette tip to minimize spontaneous differentiation ...
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bioRxiv - Microbiology 2020Quote: ... CD45− cells were magnetically sorted using Ter119/CD45-depletion kit (#19771; Stem Cell Technologies, Vancouver, BC). Next ...
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bioRxiv - Microbiology 2021Quote: ... and stem cells were passaged using enzyme free dissociation reagent ReLeSR™ (Stem Cell Tech#05872). Cultures were maintained in an undifferentiated state ...
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bioRxiv - Immunology 2021Quote: ... Human CD8+ T cells were cultured in ImmunoCult cell culture media (Stemcell Technologies, Cat no. 10981) supplemented with 35 ng/ml human IL-2 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified using negative magnetic isolation kit (Miltenyi Biotec Inc. or Stem Cell technologies) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... bulk CD4+ T cells were isolated using the EasySep CD4+ T cell Enrichment Kit (StemCell Technologies). Isolated CD4+ T cells were maintained in RPMI medium supplemented with 10% fetal bovine serum and 1% penicillin and streptomycin (R10 medium) ...
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bioRxiv - Cancer Biology 2020Quote: ... Then cells were re-suspended in 500μl and counted in 500μl ammonium chloride (Stem Cell Technologies) using the Vicell XR (Beckman Coulter) ...
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bioRxiv - Developmental Biology 2022Quote: ... at which point cells were split into new wells using ReLeSR passaging reagent (Stem Cell Technologies) with 2 μM ROCK inhibitor (Thiazovivin/TZV ...
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bioRxiv - Cancer Biology 2022Quote: Bone marrow cells were subjected to RBC lysis using Ammonium Chloride lysis buffer (Stem Cell Technologies), then incubated with anti-mouse direct lineage depletion cocktail (Miltenyi ...
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bioRxiv - Immunology 2022Quote: NK cells from spleen were enriched by the EasySep Mouse NK cell isolation kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Sorted cells were plated in triplicate in semi-solid methylcellulose medium (Methocult M3434; Stem Cell Technologies) at 1000 cells/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was changed every day and cells were passaged using gentle cell dissociation reagent (Stemcell Technologies) or singularised using accutase (Stem Cell Technologies) ...
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bioRxiv - Immunology 2019Quote: ... Bulk T cells were isolated from PBMCs using Easysep Human T Cell Isolation Kit (STEMCELL Technologies) and activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were enriched using an EasySep magnetic negative selection kit (Stem Cell Technologies #19853) as per the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... All CD19+ B cells were isolated (STEMCELL Technologies EasySep Mouse B Cell Isolation kit, STEMCELL Technologies) and mRNA was extracted and purified (PureLink RNA Mini Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... RBC-lysed 50,000 whole bone marrow cells were seeded in 1.5mL MethoCult M3434 (Stem Cell Technologies) with no additional supplemental cytokines in triplicate on 6 well plates and scored on day 8 ...
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bioRxiv - Bioengineering 2020Quote: ... at which point cells were split into new wells using ReLeSR passaging reagent (Stem Cell Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then treated with 2ml of 0.25% Trypsin/EDTA solution (Stem Cell Technologies, cat #07901) and 2ml of 5mg/mL dispase (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: Unencapsulated 50 cell aggregates were created by dissociation of human iPSCs with Accutase (STEM CELL Technologies) and reseeding in mTeSR™-1 medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... Primary NK cells were separated from donor PBMCs via magnetic bead negative selection (Stem Cell technologies). 96well ELISA plates (Nunc Maxisorp ...
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bioRxiv - Immunology 2021Quote: ... PE positive cells were enriched using an EasyEights™ magnet (Stem Cell Technologies, Cat no. 18103). Three rounds of separation were performed in total and cells were washed in complete media prior to maintenance in culture with complete RPMI + IL-2 ...
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bioRxiv - Immunology 2021Quote: ... EasySep Mouse Naive CD4+ T cell and CD8+ T cell Isolation Kit (StemCell Technologies, Vancouer, Canada) were purchased.
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bioRxiv - Cell Biology 2021Quote: ... CD34+ cells (1 × 105/mL) were suspended in serum-free expansion medium II (Stem Cell Technologies) with 50 ng/mL stem cell factor (PeproTech ...
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bioRxiv - Developmental Biology 2021Quote: ... feeder-free maintenance medium (mTeSR for human ES and iPS cells, Stem Cell Technologies, cat#85850) as previously described (Low et al. ...
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bioRxiv - Neuroscience 2021Quote: ... hiPSCs were detached from the Vitronectin XF substrate using Gentle Cell Dissociation Reagent (Stem Cell Technologies), centrifuged ...
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bioRxiv - Immunology 2021Quote: ... CD3+ T cells or CD4+ T cells were isolated via Lymphoprep and RosetteSep (both STEMCELL Technologies). For Treg isolation ...
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bioRxiv - Immunology 2020Quote: ... T-cells were obtained from PBMCs using the EasySep Human T-cell Isolation Kit (Stemcell Technologies). MLRs were set up in 96 well plates ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated with EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies, 17952).
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bioRxiv - Immunology 2021Quote: ... cells were washed in PBS and incubated in PBS + 50 nM dasatinib (Stem Cell Technologies, Canada) + DNAse at 37°C for 30 min ...
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bioRxiv - Genomics 2021Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and T cells isolated using the EasySep Human T Cell Isolation kit (Stemcell Technologies, #17951). Primary human T cells were cultured for up to 20 days in ATCC-modified RPMI (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells from healthy donors were purified from leukopaks purchased from Stem Cell Technologies using Ficoll-Paque PLUS (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: CD3ε+ T cells were isolated using negative selection Human T cell Isolation Kit (StemCell Technology, #17951). Cells were plated at 1 × 106 cells/ml in T cell growth media (StemCell Technology ...
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bioRxiv - Developmental Biology 2022Quote: ... single cell suspensions were prepared by treatment of hES cells with accutase (#07920, StemCell Technologies Inc.) and 1 × 106 cells were seeded per well of an Aggrewell™800 plate in APEL2 medium supplemented with 10 μM Y-27632 ROCK inhibitor (#72307 ...
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bioRxiv - Immunology 2022Quote: ... NK cells isolated from buffy coats using an EasySep human NK cell enrichment kit (StemCell Technologies) were added to the mixed target population at a 1:1 effector:target ratio ...
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bioRxiv - Physiology 2023Quote: ... Cells were washed with 1x PBS and plated in methocullulose media (Methocult M3434, Stem Cell Technologies). Cells were plated (4 × 105 cells per 35 mm-tissue culture dish ...
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bioRxiv - Immunology 2022Quote: ... NK cells were enriched from PBMCs by EasySep™ Human NK Cell Isolation Kit (StemCell Technologies) and cultured at 37 °C in 5% CO2 in RPMI 1640 (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... at which point cells were split into new wells using ReLeSR passaging reagent (Stem Cell Technologies). Accutase (Innovative Cell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were purified by negative selection using RosetteSepTM Human T cell enrichment cocktail (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The CD34+ cells were purified from PBMNCs by the magnetic separation method (EasySep, Stem Cell Technologies) and were cryopreserved ...
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bioRxiv - Neuroscience 2023Quote: ... Single clones were generated by seeding single cells in presence of CloneR (05888, Stem Cell Tech). All clones and the bulk population were routinely subjected to mycoplasma testing ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell clusters were released from Matrigel by incubation in dispase 5U/mL (Stem Cell Technologies 07913) for up to 30 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... human CD4+ T cells were purified from frozen peripheral blood mono-nuclear cells (PBMCs) (STEMCELL Technologies) with EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies) ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... HUDEP-2 cells were expanded in a basal medium composed of StemSpan SFEM (Stem Cell Technologies) supplemented with 10−6 M dexamethasone (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... The cells were then transduced with barcode lentivirus library (31) in StemSpan medium (Stem Cell Technologies) by centrifugation at 900 ×g for 1.5h at room temp ...
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bioRxiv - Cancer Biology 2023Quote: B cell isolation was performed using the the EasySep Human B Cell Isolation Kit (StemCell Technologies). PBMC were diluted in PBS supplemented with 2 % FBS and 1 mM EDTA to a final concentration of 107 cells/ml ...