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Citations for Stemcell Technologies :
401 - 427 of 427 citations for Polystyrene Latex Beads 5 um since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with cardiomyocytes differentiation basic medium (2 mM L-glutamine RPMI-1640 plus 1x B27 minus insulin) containing 5 μM CHIR99021 (STEMCELL Technologies, Canada) for 48 hours ...
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bioRxiv - Synthetic Biology 2024Quote: WTC11 iPSCs90 were grown with 5% CO2 at 37°C cultured in mTeSR Plus Basal Medium (Stemcell technologies; Cat. No. 100-0276) on Greiner Cellstar plates (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... BMDCs were washed and stained for flow cytometry or co-cultured at a 1:5 ratio with CFDA-SE (Stemcell Technologies, Canada) -labelled CD4 T cells isolated from a B6 ...
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bioRxiv - Biophysics 2024Quote: ... Labeled ASCs were suspended in 2 mL PBS and incubated for 5 minutes in an EasySep™ magnet (Stemcell Technologies, Vancouver, CAN) to separate the iron-labeled cells from the unlabeled.
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bioRxiv - Bioengineering 2024Quote: ... Non-treated petri dishes of 100 mm diameter were coated with 5 mL of anti-adherence rinsing solution (STEMCELL Technologies, Vancouver, CA) for 5 minutes at 37°C to prevent cell adhesion ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were passaged with Versene solution (Paneco, Russia) at a ratio of 1:15 – 1:20 every 4–5 days with ROCK-inhibitor (Stem Cell Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... or β-thalassemia patients were cultured for 14-16d at 37°C and 5% CO2 in SFEM II medium (STEMCELL Technologies, Vancouver, Canada) as previously described37,38 ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were pelleted at 350x g for 2 min and transferred onto a 6-well transwell membrane (Stem Cell Technologies, #3450). Pellets were incubated with CHIR99021 (5 µM ...
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bioRxiv - Physiology 2020Quote: ... and maintained at 37 °C with 5% CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver, Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: Primary mouse clonogenic progenitor assays or colony forming units (CFU) assays were performed by plating 5×105 unfractionated nucleated BM cells in methylcellulose M3434 (StemCell Technologies, Vancouver, Canada) in 1 ml duplicates ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
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bioRxiv - Biophysics 2020Quote: ... were grown and maintained at 37°C in hypoxic conditions (3% O2, 5% CO2) in NeuroCult™ NS-A Proliferation medium (STEMCELL Technologies, 05751) supplemented with 1% Heparin Solution (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
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bioRxiv - Molecular Biology 2023Quote: ... 2.5% and 5% (v/v) of the hydrophilic concentrate BMGLS in PneumaCult™ ExPlus or PneumaCult™ ALI medium (Stemcell Technologies, Vancouver, Canada) supplemented with MycoZAP (Lonza ...
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bioRxiv - Molecular Biology 2023Quote: ... Medium was replaced every two days and cells were split every 5 to 6 days using Gentle Dissociation reagent (STEMCELL Technologies, Vancouver, Canada). hES cells were routinely tested for Mycoplasma using the Mycoplasma PCR Detection kit (Applied Biological Materials ...
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bioRxiv - Cancer Biology 2024Quote: ... NK cells were stained with CellTrackerTM Green CMFDA (5-chloromethylfluorescein diacetate) (InvitrogenTM C7025) diluted to 10 µM in serum free ImmunoCultTM NK Cell Base Medium (STEMCELL Technologies 100-0712) for 30 min ...
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bioRxiv - Immunology 2024Quote: ... the pooled blood was carefully layered over 5 mL of Ficoll-Paque PREMIUM 1.084 (Cytiva, Uppsala, Sweden) in 15 mL SepMate-50 tubes (STEM Cell Technology, Cambridge, MA). PBMCs were collected from the interphase layer after centrifugation at 1200xg for 30 min.
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bioRxiv - Genomics 2024Quote: ... hESCs were fed every day for mTeSR1 or every 2 days for mTeSR Plus and passaged every 5–6 days using ReLeSR (Stem Cell Technologies 05872).
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bioRxiv - Synthetic Biology 2024Quote: ... HSPCs derived from healthy patients or iPSC-derived HPCs were cultured for 14d at 37°C and 5% CO2 in SFEMII medium (STEMCELL Technologies, Vancouver, Canada) as previously described19,20 ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: Mouse Intestinal Organoids were incubated in 24-well cell culture plates at 37 °C under 5% CO2 in IntestiCult Complete Organoid Medium (#06005, Stem Cell Technologies, Canada) containing CHIR-99021 (3 μM final concentration ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10,000 cells were plated per 12-well plate well onto 5% biolaminin 521 LN and differentiation started 6 hrs post plating in TeSR-E6 (Stem Cell Technologies, 05946) with CHIR99021 (Tocris ...
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bioRxiv - Bioengineering 2024Quote: ... These cells were maintained in a 5% CO2 incubator at 37°C and were fed every 24 to 48 hours with mTeSR Plus medium (StemCell Technologies, 100-0276). The iPSCs were passaged using the StemPro EZPassage tool (Life Technologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... in borate buffer for primary culture cells or Poly-D-Lysine for cells from an iPSC background before being coated with either 10 µg/ml mouse laminin or 5 µg/ml human 521 Laminin (Stemcell Technologies Australia, Melbourne, Australia) respectively to facilitate cell adhesion.
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bioRxiv - Microbiology 2021Quote: ... the isolated intestinal tissue was cut into ∼3-to 5-mm pieces and incubated in Gentle Cell Dissociation Reagent (Stem Cell Technologies, Vancouver, Canada). After removal of the Gentle Cell Dissociation Reagent ...
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bioRxiv - Developmental Biology 2022Quote: ... Roughly separated tubules were incubated in collagenase type IV in DMEM (5 mL for 1 testis pair, 1 mg/mL, STEMCELL Technologies Inc., Cat# 07909) at 37 °C while rotating ...
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bioRxiv - Developmental Biology 2024Quote: ... and the organoid pellet was resuspended in 5-10 mL pre-cooled basal DMEM/F-12 medium containing 15 mM HEPES (Stem Cell Technologies, cat.# 36254) using a 10 mL pipette pre-wetted with 1% (vol/vol ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were kept in a 37°C tissue culture incubator with 5% CO2 and lines were maintained in mTeSR Plus cultured media (Stemcell Technologies Cat# 100-1130). Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL ...