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Citations for Stemcell Technologies :
101 - 150 of 427 citations for Polystyrene Latex Beads 5 um since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... and 5 μM MG132 (Stem Cell Technologies, Cambridge, UK)) was added similar to (Huang et al ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... and magnetic bead selection using an EasySep™ Human CD34 Positive Selection Kit (STEMCELL Technologies Inc.). The resulting CD34+ HSPCs were cultured in HSPC media ...
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bioRxiv - Immunology 2020Quote: ... Primary NK cells were separated from donor PBMCs via magnetic bead negative selection (Stem Cell technologies). 96well ELISA plates (Nunc Maxisorp ...
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bioRxiv - Immunology 2022Quote: ... NGFR+ cells were bead-purified by an EasySep Human CD271 Positive Selection Kit II (STEMCELL Technologies). See Fig ...
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bioRxiv - Cell Biology 2024Quote: ... cells and beads were resuspended in complete medium with 12 or 20% OptiPrep (STEMCELL Technologies, UK) to limit object sedimentation during loading ...
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bioRxiv - Immunology 2024Quote: ... dead cells were removed by AnnexinV-based magnetic bead selection following the manufactureŕs instructions (Stemcell Technologies). In short ...
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bioRxiv - Immunology 2024Quote: Esophageal biopsies were dissociated (27) and CD45+ cells were removed using positive isolation beads (STEMCELL TECHNOLOGIES). The untouched epithelial-enriched cell fraction was washed with PBS ...
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bioRxiv - Immunology 2023Quote: ... Basophils were purified to >99% using negative selection antibodies and beads (STEMCELL Technologies, Cambridge, MA, USA).22 The pDC were prepared from the BDC suspension using BDCA-4+ selection after conducting 2 cycles of CD14+ selection to first remove monocytes.23
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bioRxiv - Immunology 2024Quote: ... samples were enriched for bead-bound cells on magnetized columns (StemCell Technologies #17684 and #17681, respectively). Samples then underwent surface staining on ice with antibodies as described above ...
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bioRxiv - Cell Biology 2023Quote: ... PBMCs were cultured at 37°C under 5% CO2 for 5 days in ImmunoCult™-XF T Cell Expansion Medium (STEM CELL Technologies) supplemented with 30 ng/mL human recombinant IL-2 (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... we performed a Cut and Run/Q-PCR (C&R/Q-PCR) experiment of 5×105 untreated cells and 5×105 cells treated for 30 minutes with recombinant human INFγ (STEMCELL Technologies, Vancouver, BC, Canada). For the C&R procedure ...
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bioRxiv - Microbiology 2020Quote: ... and 5 µM A83-01 (all from Stem Cell Technologies). Expansion media was replaced every 3-4 days ...
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bioRxiv - Cell Biology 2021Quote: ... 5% CO2 (MegaCult®-C Medium without Cytokines, STEMCELL Technologies). Cultures were supplemented with human recombinant 50 ng/ml TPO (Peprotech) ...
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bioRxiv - Neuroscience 2023Quote: ... and passaging every 4-5 days using ReLeSR (StemCell technologies). hES-qualified Matrigel (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended in 5 U/mL dispase (StemCell Technologies, 07913) with 0.1 mg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Doxycycline (Cas No.24390-14-5) from Stem Cell Technologies, and SGK1 inhibitor GS-9007 (Cas No.1426214-51-8 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 5 U/ml dispase (Stem Cell Technologies, 07913) and 50 ug/ml DNase I (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were treated with 5 µM Ionomycin (STEMCELL Technologies). hHOs were generated from hiPSC-L1 ...
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bioRxiv - Immunology 2020Quote: ... CAR-transduced T cells were enriched by positive selections using anti-CD19 magnetic beads (Stem Cell Technologies). Cultures were propagated for 14-16 days before applying to assays or get cryo-preserved ...
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bioRxiv - Cancer Biology 2022Quote: ... CD14+ monocytes were isolated from normal donor peripheral blood by magnetic bead isolation (EasySep, Stem Cell Technologies) then co-cultured with PFA EPN cell lines MAF-811 and MAF-928 ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were negatively purified with EasySepTM beads (human T-cell isolation kit, STEMCELL Technologies, Vancouver, Canada). T cells were cultured in Advanced RPMI 1640 enriched with FBS (10%) ...
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bioRxiv - Immunology 2024Quote: ... CD24+ cells were depleted using mouse streptavidin magnetic beads (anti-mouse Rapidspheres, cat no 19860, STEMCELL Technologies) following the manufacturer’s guidelines as previously described (29 ...
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bioRxiv - Immunology 2024Quote: ... which involved magnetic bead separation employing EasySep Direct Human Monocyte Isolation Kit (StemCell Technologies, Vancouver, BC, CAN). This technique ensured the attainment of a highly pure monocyte population with minimal contamination from other cell types ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells and CD19+ B cells were negatively isolated using magnetic bead based separation (EasySep, StemCell Technologies). CD14+ monocytes were positively selected (EasySep ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by CD34 magnetic bead-based enrichment using the EasySep human CD34 positive selection kit II (StemCell technologies). We found this enrichment strategy to be simple and reproducible and chose it for several reasons ...
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bioRxiv - Cell Biology 2022Quote: ... IPSCs were incubated with 5 µm of RockIn (STEMCELL Technologies, Inc.) for 2-3 h before dissociating with Accutase ...
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bioRxiv - Bioengineering 2022Quote: ... then with 5 μM A8301 (Stem Cell Technologies, catalogue no. 72022), 20 ng/mL HGF (Peprotech ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3–5 days with ReLeSR (STEMCELL Technologies). All cells used had a normal diploid karyotype.
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bioRxiv - Bioengineering 2024Quote: ... On day 4 of differentiation 5 μM IWP4 (Stem Cell Technologies), 2.5 ng/ml BMP4 ...
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bioRxiv - Immunology 2020Quote: CD4 or CD8 T cells were isolated from spleen using magnetic beads as described by manufacturer’s protocol (STEMCELL technologies). 4000 cells in ratio 1:1 were transferred into WT mice 1 day prior to LCMV-Cl13 infection.
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from splenocytes of OT-I mice using a magnetic bead isolation kit (Stemcell Technology) and cultured following a previously published protocol (44) ...
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bioRxiv - Immunology 2024Quote: ... followed by depletion of non-NK lineages using immuno-magnetic beads (EasySep human NK cell enrichment kit, Stemcell Technologies). Samples were sequenced in dedicated flow cells on a HiSeq 2500 (Illumina ...
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bioRxiv - Systems Biology 2020Quote: ... followed by trituration in 5 U/mL dispase (Stem Cell Technologies 07913) plus 1 mg/mL DNase I (Stem Cell Technologies 07900 ...
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bioRxiv - Immunology 2020Quote: ... anti-CD38-FITC (clone AT1; 5 μL; cat. # 60131FI) from STEMCELL Technologies; and anti-CXCR5-PE (clone MU5UBEE ...
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bioRxiv - Cancer Biology 2020Quote: 5 × 105 nucleated splenocytes were cultured with Methocult M3434 medium (STEMCELL Technologies) supplemented with human thrombopoietin (50 ng/ml) ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 ml of mouse Neurocult Proliferation Supplement (Stem Cell Technologies, Catalog# 05701), 50 μL of 20 μg/ml EGF (Peprotech Inc ...
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bioRxiv - Immunology 2020Quote: ... and 5 ng/mL recombinant human IL-15 (StemCell Technologies, Cat # 78031). Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 5 nM Y-27632 dihydrochloride ROCK inhibitor (Stem Cell Technologies). The iPSCs were subsequently fed daily for 3 consecutive days with Neuronal Induction Medium (DMEM/F12 medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... anti-CD38-FITC (clone AT1; 5 μL; cat. # 60131FI) from STEMCELL Technologies; and anti-CXCR5-PE (clone MU5UBEE ...
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bioRxiv - Biochemistry 2022Quote: ... 5×102 HSPCs were seeded in 1.5ml of Methocult Optimum (STEMCELL Technologies) and after 14 days ...
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bioRxiv - Microbiology 2024Quote: ... medium was replaced by ODM supplemented with 5 µM CHIR99021 (StemCell technologies) and 10 µM Y-27632 ...
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bioRxiv - Developmental Biology 2023Quote: ... the medium was supplemented with 5 or 10mM CHIR99021 (Stemcell technologies 72052), or with 200 ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with Hydrocortisone stock solution (5 μl/ml; Stem cell technologies #07925) and Heparin solution (2 μl/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were passaged using a 5 min incubation with Accutase (StemCell Technologies) on Matrigel-coated plates in mTeSR1 media supplemented with 10 μM of Y-27632 (Cayman Chemicals) ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µM (for iPSC) or 5 µM (for ESC) XAV939 (Stemcell Technologies), and 10 µM Y27632 ...
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bioRxiv - Biochemistry 2023Quote: ... cells were further dissociated in Dispase 5 U/mL (STEMCELL Technologies, 07913) plus 50 µg/mL DNase I (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... B27GB medium was supplemented with 5 ng/mL GDNF (78058, Stemcell Technologies) and 5 ng/mL BDNF (ref ...
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bioRxiv - Cell Biology 2024Quote: ... 5% CO2 for 2 weeks in supplemented mTeSR medium (STEMCELL Technologies #85850). For KO of C3orf58 or SLC39A9 in iPSC ...
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bioRxiv - Cell Biology 2024Quote: ... followed by basic medium containing 5 μM IWR-1-endo (STEMCELL Technologies) for another 48 h ...
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bioRxiv - Biophysics 2021Quote: ... the beads were removed carefully and the agarose wells were coated with a non-adhesive coating solution (07010, Stemcell Technologies) for 30 min at room temperature (RT) ...