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Citations for Stemcell Technologies :
401 - 450 of 4781 citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Media was changed daily and cells were passaged using ReLeSR (Stem Cell Technologies). For differentiation ...
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bioRxiv - Genomics 2023Quote: H9 cells were treated with 10 uM Y-27632 (Stem Cell Technologies, 72304) for at least 2 h prior to nucleofection ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 6h in hypoxia incubator chamber (Stem cell Technologies, #27310) at 37°C and 1% CO2.
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bioRxiv - Developmental Biology 2023Quote: ... cells were placed in mTeSR Plus supplemented with CloneR (Stem Cell Technologies, #05888) for 36 hours and then selected in 250 ng/ml puromycin for 36 hours ...
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bioRxiv - Immunology 2023Quote: ... Isolated T cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stem Cell Technologies).
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bioRxiv - Microbiology 2023Quote: ... Cells were detached from a T75 using Gentle Cell Dissociation Reagent (Stemcell Technologies) and recovered in DMEM prior to staining ...
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bioRxiv - Immunology 2023Quote: ... B cells were enriched (Human Pan-B cell Enrichment Kit [19554, StemCell Technologies]) and then stained with viability dye FVS780 (565388 ...
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bioRxiv - Neuroscience 2023Quote: ... Stem cells were passaged as needed using ReLeSR medium (Stem Cell Technologies, 05872). Two control fibroblast lines GM03651 and GM03652 were purchased from Coriell and reprogrammed to pluripotency using Sendai viral vectors containing OCT4 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells was spun down and resuspended in 10U Dispase (Stem Cell Technologies, #07923) and 1000U DNase (Worthington ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were further cultured with PneumaCult™-Ex Plus Medium (Stem Cell Technologies) on the upper and lower chamber 48 hours ...
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bioRxiv - Biophysics 2023Quote: ... cells were treated at room temperature with ReLeSR (Stem Cell Technologies, Cat#: 05872) for 1 minute followed by 5 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: Induced pluripotent stem cells were maintained in mTeSR+ medium (Stem Cell Technologies, 05825) on plates pre-coated with Matrigel (Corning ...
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bioRxiv - Genetics 2023Quote: Transfected cells were dissociated into single cells with Accutase (STEMCELL Technologies, Cat#07920) and centrifuged for 4 minutes at 200 x g ...
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bioRxiv - Bioengineering 2023Quote: ... were cultured in ImmunoCult XF T Cell Expansion media (Stem Cell Technologies, 10981) at 37 °C with 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... were cultured in ImmunoCult-XF T Cell Expansion media (Stem Cell Technologies, 10981) at 37 °C with 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... 2.5 mil cells were plated into 24-well AggreWell800 plates (Stem Cell Technologies; pre-treated with Anti-Adherence rinsing solution ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured for 2 days in StemSpan (Stem Cell Technologies, Cat. 09655) supplemented with TPO (100ng/mL) ...
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bioRxiv - Microbiology 2024Quote: ... M2-10B4 and S1/S1 stromal cells were obtained from Stem Cell Technologies and maintained in DMEM with 10% FBS and penicillin ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were maintained in mTeSR media (Stem Cell Technologies, catalog no. 85850), which was replenished daily ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were dissociated into a single-cell solution with Accutase (STEMCELL Technologies), and 9,000 live cells were seeded in each well of U-bottom Ultralow attachment 96-well plates (Corning ...
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High-resolution profiling reveals coupled transcriptional and translational regulation of transgenesbioRxiv - Synthetic Biology 2024Quote: ... iPS11 cells were dissociated using Gentle Cell Dissociation Reagent (STEMCELL Technologies, 100-1077) according to manufacturer’s instructions and counted using a hemocytometer ...
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bioRxiv - Neuroscience 2024Quote: ... PSCs were dissociated to single cells using Gentle Cell Dissociation Reagent (StemCell Technologies) and plated in AggreWell™800 culture plates (StemCell Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... cultures were incubated in Accutase cell detachment medium (Stem Cell Technologies, 07922, 07920) at 37 °C for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... For cryopreservation cells were resuspended in CryoStor ® (Stem Cell Technologies, 100-1061) and frozen at - 80 °C.
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bioRxiv - Immunology 2024Quote: ... Live cells were enriched using the EasySep Dead Cell Removal kit (Stemcell Technologies). For scRNAseq of pre-enriched monocytes ...
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bioRxiv - Bioengineering 2024Quote: ... cell aggregates were gently detached using Gentle Cell Dissociation Reagent (GCDR, STEMCELL Technologies). To prevent spontaneous differentiation ...
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bioRxiv - Cell Biology 2024Quote: 500 freshly sorted cells were seeded in MethoCult GF M3434 (Stem Cell Technologies) and colonies were scored after 10 days ...
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bioRxiv - Cancer Biology 2024Quote: ... CD8+ T cells were isolated from human peripheral blood mononuclear cells (STEMCELL Technologies) using the EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2024Quote: HUES8 human embryonic stem cells (hESCs) were cultured mTeSR1 (Stem Cell Technologies, 05850) media and incubated at 37 °C with 5% CO2 ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium was changed daily and the cells were passaged every 4–8 days depending on confluency using Gentle Cell Dissociation Reagent (Stem Cell Technologies). 10 μM of ROCKi (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... and iEndo-mKate2 PGP1 cells were dissociated from 70%-80% confluency by incubating cells in Gentle Cell Dissociation Reagent (STEMCELL Technologies, #07174) for 12 min at 37°C ...
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bioRxiv - Immunology 2021Quote: NK cells were purified from uninfected spleens of WT and CypD-/- mice using the EasySep Mouse NK Cell Isolation Kit (Stem Cell Technologies) according to the supplier’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... C57BL/6.OT-I.Ubiquitin-GFP CD8+ T cells were enriched from single-cell suspensions of lymph nodes and spleens using mouse CD8-negative selection kits (Stem Cell Technologies). Enriched CD8 T cells (5×106 ...
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bioRxiv - Cancer Biology 2020Quote: ... Splenocytes were fused with SP2/0 Ag14 myeloma cells (Kohler and Milstein, 1975) and hybrid cells were selected by growth in methylcellulose-containing HAT medium (Stem Cell Technologies). Approximately 600 clones were isolated and transferred to liquid HT medium in 96-well plates ...
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bioRxiv - Cell Biology 2021Quote: ... DKC1 A353V (AAVS1-EGFP) iPS cells and DKC1 A353V mutation corrected (AAVS1-EGFP) iPS cells) were maintained on vitronectin (Stem Cell Tech.) coated plates using StemMACS™ iPS-Brew XF medium (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... Passage one cells were seeded into T75 cell culture flasks (250,000 cells/flask) and expanded in PneumaCult ExPlus medium (StemCell Technologies, Vancouver, Canada) at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... cells were sorted for single cells for transfected (GFP+) cells and plated into prewarmed (37C) StemFlex media supplemented with 1X CloneR (Stem Cell Technologies) onto Vitronectin XF (Stem Cell Technologies ...
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bioRxiv - Immunology 2022Quote: ... Naïve human CD4+ T cells were isolated using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
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bioRxiv - Immunology 2020Quote: ... and CD8+ T cells were negatively isolated using the EasySep™ Mouse CD8+ T cell Isolation Kit (Stem Cell Technologies, Grenoble, France) or MojoSort™ Isolation Kits (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Purity and viability of isolated T cells was assessed using flow cytometry prior to cryopreservation of isolated T cells in CryoStore CS10 cryopreservation media (Stem Cell Technologies) at a density of 1-1.5×107 cells per ml.
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bioRxiv - Cancer Biology 2022Quote: ... pH 7.3] to remove red blood cells and CD8+ T cells were isolated via EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies,19853) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... via Ficoll-Paque (Cytavia) gradient selection for peripheral blood mono-nuclear cells followed by human CD8+ T cell negative magnetic selection (Stem Cell Technologies). Primary CD8+ T cells were thawed in supplemented RPMI 1640 2-12 hours prior to the start of experiments.
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ HSPCs were then seeded into 96-well round bottom plates (cell density at 100,000 (100k) cells/100 μL) and prestimulated in StemSpanTM SFEM (Stem Cell Technologies #09650) supplemented with StemSpan™ CC100 (Stem Cell Technologies #02690) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were plated for single cell clones by plating 1000 cells per 10cm2 dish on Synthemax with 1x CloneR (Stemcell technologies #05888), and single cell clones were genotyped by PCR for correct integration before sequencing both alleles from positive clones.
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bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
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bioRxiv - Immunology 2023Quote: ... T cells from the transgenic mouse splenocytes were enriched by magnetic isolation using the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies). T cells were centrifuged and resuspended in PBS ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC-derived DA neurons were plated on a 48-well Classic MEA plate (Axion BioSystems) at a cell density of 1.2×105 cells per well and maintained in culture using Complete BrainPhys™ (Stem Cell Technologies). Culture Medium prepared as directed in the iCell DopaNeurons MEA Application Protocol (https://www.fujifilmcdi.com/) ...