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Citations for Stemcell Technologies :
451 - 500 of 4781 citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Accutase was quenched and single cells were seeded for differentiation in suspension at a density of 1×106 cells/mL in complete mTeSR media (Stem Cell Technologies) supplemented with 10ng/mL FGF2 (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... apoptotic and dead cells were removed by immunomagnetic cell separation using the Annexin Dead Cell Removal Kit (StemCell Technologies, cat. no. 17899) and EasySep Magnet (StemCell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ HSPCs were then seeded into 96-well round bottom plates (cell density at 100,000 (100k) cells/100 μL) and prestimulated in StemSpanTM SFEM (Stem Cell Technologies #09650) supplemented with StemSpan™ CC100 (Stem Cell Technologies #02690) ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells that passed through 70-uM cell strainer were purified with EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies Inc). For T-cell proliferation assays ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells and isolated CD4+ T cells were activated using Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies, 10970) per manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... pH 7.3] to remove red blood cells and CD8+ T cells were isolated via EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies,19853) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... via Ficoll-Paque (Cytavia) gradient selection for peripheral blood mono-nuclear cells followed by human CD8+ T cell negative magnetic selection (Stem Cell Technologies). Primary CD8+ T cells were thawed in supplemented RPMI 1640 2-12 hours prior to the start of experiments.
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6low and CCR6high memory subgroups and cultured at 1 × 106 cells/ml in 24 well plates in ImmunoCult XF T cell expansion medium (Stem Cell Technologies) supplemented with 10% FBS and IL-2 (200 units/ml) ...
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bioRxiv - Immunology 2024Quote: ... B-cells were then purified from the other harvested PBMCs by negative selection using a Human B-cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Brachial and Axillary) and CD4+ve T cells were isolated using EasySep™ Mouse CD4+ T Cell Isolation Kit (STEM CELL TECH). Protein lysate of snap-freeze tissue in liquid nitrogen was prepared by homogenizing 5mg tissue in 500μl ice-cold RIPA lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Colony formation assays were established using primary AML BM cells cultured at 1×105 cells/ml in MethoCult-H4434 (Stem Cell Technologies) in the presence of vehicle or drug ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples for SLAM population analysis and cell sorting were first subjected to lineage-negative population enrichment using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Preparation of cells: Human CD4+ cells were isolated from buffy coats using EasySep™ Human CD4+ T Cell Isolation Kit (Stemcell Tech, # 17952), resuspended with complete RPMI (cRPMI ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
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bioRxiv - Developmental Biology 2022Quote: ... NPC-iPS cells were treated with 10 µM Rho kinase inhibitor Y-27632 overnight before dissociated to single cells with Accutase (Stem Cell Technology 07922). About 10 million cells were electroporated with 10 μg of AAVS1 zinc finger nuclease-encoding plasmid and 30 μg of AAVS1-tdTomato with one electroporation of 250 V ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
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bioRxiv - Immunology 2021Quote: Purified CD4+CD25− T cells were magnetically isolated from spleen and peripheral lymph node mononuclear cells using an EasySep magnetic T cell negative isolation kit (Stem Cell Technologies, Inc.) with addition of a biotin anti-mouse CD25 antibody (0.5 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated from the pooled cell suspensions using an EasySep(tm) Mouse CD4+ T Cell Isolation Kit from Stemcell Technologies (Vancouver, Canada) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The irradiated negative fraction was passed through a 70uM filter to create a single-cell suspension prior to counting and seeding at 2e6 cells per nanobioreactor in StemPro media (Stem Cell Technologies Inc). The CD34+ fraction was resuspended in 1X StemPro-34 SFM medium supplemented with a cytokine cocktail of SCF (50ng/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... 600K CD34+ Fucci2BL transduced cells were seeded into nanobioreactors pre-cultured with the allogenic CD34- cell fraction and StemPro media (Stem Cell Technologies Inc.).
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bioRxiv - Developmental Biology 2022Quote: ... by plating them at 125.000 cells/cm2 on Matrigel and growing the cells in STEMdiff™ Neuron Differentiation Kit (Stem Cell Technologies, #08500) medium for one week with daily medium changes.
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bioRxiv - Microbiology 2024Quote: ... CD4+ T cells were isolated from frozen PBMC tubes using the EasySep™ Human CD4+ T Cell Isolation Kit (Stem Cell Technologies, #17952) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... human PBMCs (AllCells) were thawed and CD4+ T cells were isolated using the EasySep™ Human CD4+ T Cell Isolation Kit (Stem Cell Technologies). Naive CD4+ T cells were resuspended in complete RPMI (cRPMI ...
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bioRxiv - Neuroscience 2024Quote: ... to form embryoid bodies 1.5X 106 iPSC cells were cultured in EB microglia media (Stem Cell Base Media (Stem Cell Technology #100-1130) +100ng/mL VEGF (Proteintech #HZ-1038)+40ng/mL SCF (Peprotech #300-07)+100ng/mL BMP4 (Peprotech #120-05) ...
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bioRxiv - Bioengineering 2024Quote: ... single-cell suspensions were depleted of hematopoietic cells committed to a specific lineage using a Lineage Cell Depletion Kit (EasySep, StemCell Technologies, Inc., 19856A), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The 1157.2 human iPS cell line (Boston Children’s Hospital Stem Cell Core) was maintained in StemFlex media (Stem Cell Technologies cat. no. A3349401), whereas the PGP1 iNGN iPS cell line (a gift from George Church at Harvard Medical School ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples with <70% viability and >100K cells were enriched for viable cells using the EasySep Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, cat# 17899). The live cell-enriched population was pelleted (350xg ...
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bioRxiv - Developmental Biology 2024Quote: ... and cells plated on fibronectin-coated 96-well plates at 20 000 cells / well in Neuro-Cult Proliferation solution (Stem Cell Technologies 05702) with heparin (Stem Cell Technologies 07980) ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated using a mouse B cell isolation kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: Human embryonic stem cells (hESCs) were routinely cultured in mTeSR1 medium (Stem Cell Technologies) on growth factor-reduced Matrigel-coated dishes (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... Surviving cells were expanded and then passaged as single cells using Accutase (STEMCELL Technologies) in MEF-CM supplemented with 10 µM ROCK inhibitor to grow clonal colonies ...
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bioRxiv - Bioengineering 2020Quote: ... Bulk T cells were isolated using RosetteSep Human T cell Enrichment Cocktail (StemCell Technologies). Peripheral mononuclear blood cells (PBMCs ...
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bioRxiv - Neuroscience 2020Quote: ... iPSCs at ~70% confluence were dissociated into single cells with Accutase (Stem Cell Technologies) and 10,000 cells/well were seeded into a 96-well U-Bottom Low-Attachment plate (Corning ...
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bioRxiv - Bioengineering 2020Quote: T-cells were isolated with the EasySep Human T-cell isolation Kit (STEMCELL Technologies) from frozen human peripheral blood mononuclear cells obtained from healthy donors ...
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bioRxiv - Cancer Biology 2021Quote: ... live cells were purified using EasySep Dead Cell Removal (Annexin V) Kit (StemCell Technologies). The remaining viable cells were further incubated in complete media without CRYBMIM for 6-9 days with media replacement every 3 days ...
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bioRxiv - Cancer Biology 2020Quote: ... 25,000 cells were plated in duplicate in MethoCult GF M3434 medium (Stem Cell Technologies) and cultured at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... and then were gently suspended with gentle cell dissociation solution (Stem Cell Technologies Inc.) and incubated for 7 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... or the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The CD34+ cells were cryopreserved at 10e6 cells/mL in Cryostor CS10 (STEMCELL Technologies) according to the manufacturer’s protocol and the remaining hemogenic endothelium was cultured in 2mL of monocyte factory medium ...
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bioRxiv - Cancer Biology 2021Quote: ... and activated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Stem Cell Technologies) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... splenic B cells were purified with a mouse B cell isolation kit (StemCell Technologies), and then stimulated with lipopolysaccharide (LPS ...
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bioRxiv - Developmental Biology 2021Quote: H9 hESCs cells were routinely cultured in mTeSR1 (Stem Cell Technologies; Cat. No. 85850) and growth factor reduced Matrigel (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: Cultured cells were detached from the plates using Accutase cell detachment solution (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: CD4+ T cells were isolated using negative selection (Stem Cell Technologies CD4 enrichment kit) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... 4000 cells were plated per dish for WM cell lines (Methocult H4330 StemCell Technologies). Colonies were scored on day 7 after plating.
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bioRxiv - Genetics 2022Quote: ... cells were placed in HSC base medium (IMDM containing 20% BIT (Stem Cell Technologies), 100ng/mL recombinant human (rh ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were maintained in mTeSR Plus media with supplement (Stem Cell Technologies, Cat.#05825) at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: Human iPSC (XCL-1) derived neural progenitor cells (NPCs, Stem Cell Tech cat#70901) were maintained in serum-free STEMdiff™ Neural Progenitor Medium 2 (Stem Cell Tech cat#08560 ...
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bioRxiv - Immunology 2020Quote: ... NK cells were isolated from PBMC via negative selection (EasySep Cell Separation; StemCell Technologies), added to a mix of irradiated feeders consisting of freshly isolated allogeneic PBMC combined with log-phase-growth RPMI 8866 cells (Sigma-Aldrich ...