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Citations for Stemcell Technologies :
401 - 450 of 1011 citations for Acetamide N 5 bis 2 hydroxyethyl amino 2 2 chloro 4 6 dinitrophenyl azo 4 methoxyphenyl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Mature organoids are collected by rinsing the culture well with 1-2 ml ice cold DMEM++ (= DMEM/F12 + 15 mM HEPES (Stem Cell Technologies) + 1x GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng ml−1) and PDGF-BB (10 ng ml−1; Shenandoah) and heparin (2 ng ml−1; Stem Cell Technologies). For all patient-derived cultures ...
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bioRxiv - Immunology 2022Quote: ... blood was resuspended in PBS/2%FBS/EDTA and separated using Ficoll-Paque Plus (Cytiva, 17144002) density gradient centrifugation in SepMate50 tubes (StemCell Technologies, 85450), followed by two washes in PBS/2%FBS/EDTA ...
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bioRxiv - Bioengineering 2023Quote: ... Medium was refreshed every 2–3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... Whole blood was diluted 1:1 with phosphate-buffered saline (PBS) containing 2% FBS and then layered onto Lymphoprep (STEMCELL Technologies, 07851) in SepMate-50 isolation tubes (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
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bioRxiv - Immunology 2024Quote: B cells were isolated from 2 healthy PBMC donors using the EasySep™ Human B-cell Isolation Kit (Catalog #17954) from StemCell Technologies. Cells were then stained with APC anti-IgD-APC ...
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bioRxiv - Bioengineering 2024Quote: ... Fresh gonadal and perirenal adipose tissues were minced with scissors and incubated in collagenase type II (2 mg/mL; StemCell Technologies, CA) and DNase I (20 µg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... Purified cell populations were expanded in endothelium maintenance media consisting of conditioned media diluted at 1:1 ratio with StemPro-34 SFM supplemented with 2 µg/mL heparin (STEMCELL Technologies, 07980). Media was replaced every other day or until the conditioned media was depleted ...
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bioRxiv - Cancer Biology 2024Quote: Primary cells were seeded in 12-well plate at a density of 1×106 cells per well in 2 ml StemSpan™SFEM II media (STEMCELL Technologies) with growth factors cocktail (100 ng/ml SCF ...
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bioRxiv - Genomics 2020Quote: ... The cells were passaged every 3–4 days using Accutase (Stemcell Technologies). The iPS cells conversion to EPS as previous reported47.
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bioRxiv - Cell Biology 2024Quote: ... Passaging was performed every 3-4 days using ReLeSR (Stemcell Technologies, #05872) according to manufacturer’s instructions ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... 480 ng/mL hydrocortisone and 4 µg/mL heparin (STEMCELL Technologies, 07980). ALI cultures were grown in a 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... PneumaCult ALI medium supplemented with 4 μg/mL heparin (Stem Cell Technologies), 5 μM hydrocortisone ...
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bioRxiv - Genomics 2022Quote: ... 4 mL of 29% Iodoxanol cushion was prepared with OptiPrep (Stemcell Technologies) and diluent medium (250 mM sucrose ...
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bioRxiv - Cell Biology 2024Quote: ... Passaging was performed every 3-4 days using ReLeSR (Stemcell Technologies, #05872) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 3-4 days using Accutase (Stem Cell Technologies, # 07920) The detailed procedure can be found in previous studies28.
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC). For intracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Cell Biology 2022Quote: ... These cells were frozen as a master cell bank (passage 1) and working cell bank (passage 2) in CryoStor CS10 freezer medium (STEMCELL technologies, Vancouver, Canada), supplemented with Y-27632 (5 μM ...
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bioRxiv - Neuroscience 2024Quote: ... with a tet-on SOX9 and NFIA overexpression vector were grown to 30% confluency and then treated with small molecules SB43152 (STEMCELL Technologies; 2 µM), DMH1 (STEMCELL Technologies ...
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bioRxiv - Genomics 2024Quote: ... HPCs were plated at 22,000 cells/cm2 onto a Matrigel-coated 6-well plate containing 2ml of Microglia differentiation medium (Basal Medium with supplement 1 and 2 at 1:9 and 1:225, respectively; STEMCELL Technologies, #100-0019). Cells were supplemented with fresh half-medium every other day from day 0 to day 10 ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 100 U ml−1 IL-2 in the presence of ImmunoCult™ CD3/CD28/CD2 T cell activator (Stem Cell Technologies # 10970) in 96 well plates overnight (150 ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 20ng/mL recombinant human IL-2 and 7μL/mL of ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stem Cell Technologies, 10970) every week.
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bioRxiv - Neuroscience 2023Quote: ... non-essential amino acids (NEAA, Stemcell technologies). After 24h (day0) ...
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bioRxiv - Cell Biology 2020Quote: ... at 8.8µg/cm2 and passaged every 4 days using ReLeSR (Stem Cell Technologies). All hPSCs experiments were conducted with cells ranging from 40 and 70 passages ...
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bioRxiv - Developmental Biology 2021Quote: ... and cells were routinely passaged every 4 days using ReleSR (StemCell Technologies; 05872). CRISPRi-SOX2 cells were plated on vitronectin-coated (ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... 4 million hiPSC were seeded into an AggreWell 800 well (Stem Cell Technologies) to generate embryoid bodies (EBs ...
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bioRxiv - Bioengineering 2021Quote: ... single cells suspended in a 3:4 mixture of Mammocult (StemCell Technologies 05620) and Matrigel (Corning 354234 ...
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bioRxiv - Molecular Biology 2024Quote: ... inhibition of canonical Wnt signaling is performed using IWP-4 (#72552, STEMCELL Technologies) a Wnt signaling inhibitor ...
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bioRxiv - Cancer Biology 2023Quote: ... 5,000 cells were re-suspended in 4 mL of Methocult media (STEMCELL Technologies). 1.1 mL of methocult-cell suspension was plated into a 6-well plate using 16-gauge blunt-end needles (STEMCELL Technologies) ...
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bioRxiv - Genomics 2023Quote: ... Undifferentiated iPSCs were detached by a 4-min incubation with Accutase (StemCell technologies) and seeded onto Geltrex (Life Technologies)-coated plates at a density of 200,000 cells/cm2 ...
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bioRxiv - Neuroscience 2024Quote: ... EBs were cultured for 4 days in mTeSR™ Plus medium (STEMCELL Technologies), supplemented daily with 50 ng/mL bone morphogenetic protein 4 (BMP4) ...
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bioRxiv - Cancer Biology 2024Quote: ... edited residual HSCs were added to 4 mL of MethoCult H4435 (Stemcell Technologies) and plated in triplicate ...
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bioRxiv - Cell Biology 2020Quote: ... Interleukin-6 (10 ng/mL) and Heparin (5 U/mL) (all supplied by Stem Cell Technologies).
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bioRxiv - Neuroscience 2022Quote: ... medium was refreshed every 7 days by replacing half of the medium with Brainphys neuronal medium supplemented with 2% NeuroCult SM1 neuronal supplement (Stem cell technologies, Köln, Germany) and 1% pen/strep (BP-complete medium).
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bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were purified from donor 2 LRS cone using a Rosettesep human CD4+ enrichment cocktail (Stemcell Technologies, Cambridge, MA; Supplementary Fig. 1B). 5×106 CD4+ T cells from donor 2 were cultured with 5×105 DCreg from donor 1 in X-vivo media for 5 days ...
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bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: AGMs of E10.5 embryos were dissected in PBS with 7% fetal calf serum (FCS) and penicillin/streptomycin (100 U/mL) and cultured as explants for 2 days in medium consisting of Stemspan (Stem Cell Technologies, cat # 09600), 20% fetal calf serum ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated with antibodies against CD3/CD28 (2 µg/mL CD3 and 1 µg/mL CD28 antibodies, ImmunoCult™ Human CD3/CD28 T Cell Activator, Stemcell Technologies, or eBioscience) or PMA (20 ng/mL and ionomycin (1 µg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were fed daily and split every 4-7 days using ReLeSR (StemCell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated overnight at 4° C with anti-CD3 (Clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Genetics 2021Quote: ... iPSC cultures were passaged every 3-4 days with Accutase (Stem Cell Technologies, 07920) or ReLeSR (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... replated at a density of 1.58 × 105 cells/cm2 in Vitronectin XF-coated 24 well plate into cardiomyocyte media (RPMI, 2% B27 supplement plus insulin [Life Technologies Australia, Cat.#17504001], 10% FBS, and 10 µM Y-27362 ROCK inhibitor [Stem Cell Technologies, Cat.#72308]) and cultured overnight ...
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bioRxiv - Immunology 2020Quote: Whole blood was collected into EDTA tubes and then diluted with an equal volume of phosphate-buffered saline (PBS) + 2% fetal bovine serum (FBS) and layered over Ficoll using SepMate™-50 tubes (STEMCELL Technologies Inc., Vancouver, Canada). Cells were centrifuged at 1200×g for 10 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... hybridoma cells were washed and suspended at a concentration of 2-3 × 105 input myeloma cells per ml in semi-solid medium D (StemCell Technologies, Canada, Cat. No. 03804) supplemented with 5% heat inactivated FBS ...
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bioRxiv - Cell Biology 2022Quote: ... medium was replenished daily and were passaged every 5-6 days with Dispase I (StemCell Technologies, 07923) in small cell clumps ...