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Citations for Stemcell Technologies :
451 - 500 of 1011 citations for Acetamide N 5 bis 2 hydroxyethyl amino 2 2 chloro 4 6 dinitrophenyl azo 4 methoxyphenyl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... using base medium (1:1 ratio of DMEM/F12 Glutamax/Neurobasal medium [Gibco], 2% B27 supplement without vitamin A [Gibco], 10% BIT 9500 [StemCell Technologies], and 1 mM glutamine [Gibco]) supplemented with 20ng/ml EGF (Chemicon) ...
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bioRxiv - Genetics 2023Quote: ... the top 40% of ATTO-550 positive cells were sorted as single-cells into individual wells of two 96-well plates with different combination of CellAdhere Laminin-521 (STEMCELL Technologies, Cat#77004, (2 μg/cm2)) or Vitronectin (0.5 μg/cm2) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were passaged every 4 to 7 days with ReLeSR reagent (StemCell Technologies catalog #05872) to ensure that only undifferentiated stem cells remained in the cultures ...
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bioRxiv - Genomics 2021Quote: ... and 4) one cord blood (CB001) collected with informed consent by StemCell Technologies (catalog #70007) (Table S1) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were passaged every 3-4 days by dissociating them using Accutase (STEMCELL Technologies) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Primary cells are resuspended in a 3:4 solution of Mammocult medium (Stem Cell Technology) and Matrigel (Corning)17,26,29 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged every 4 days by non-enzymatic dissociation with ReLeSR (StemCell Technologies Inc.) and plated at a density of 5.0×105 per 60 mm plate.
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bioRxiv - Bioengineering 2023Quote: ... and passaged every 3-4 days using Gentle Cell Dissociation Reagent (STEMCELL Technologies, 100-0485) for clump passaging ...
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bioRxiv - Cancer Biology 2024Quote: 200-500 edited residual HSCs were added to 4 mL of MethoCult H4435 (Stemcell Technologies) and differentiated for 14 days ...
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bioRxiv - Genetics 2024Quote: ... every other day and passaged every 3–4 days with Accutase (Stem Cell Technologies 07920) or ReLeSR (Stem Cell Technologies 100-0483) ...
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bioRxiv - Developmental Biology 2023Quote: ... Explants were then cultured in suspension in DMEM for 2 hours at 37°C with a 1/250 dilution from 100 mM stock solution or Dispase II (Stem Cell Technologies; #07923, at 0.2 U/mL).
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bioRxiv - Cell Biology 2020Quote: ... cells were dissociated with Gentle Cell Dissociation Reagent (STEMCELL Technologies 07174 and passaged 1:4 (C1) or 1:6 (C2 ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were cultured in Mammocult media supplemented with 4 μg/ml Heparin (Stem Cell Technology), 0.5 μg/ml hydrocortisone and 10% Mammocult Proliferation Supplement (Stem Cell Technology) ...
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bioRxiv - Bioengineering 2022Quote: Human iPSCs (IMR90-4; WiCell Research Institute) were maintained with mTeSR Plus (STEMCELL Technologies, 100-0276) on dishes coated with 9-10 μg/cm2 Growth Factor Reduced Matrigel (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were passaged every 4 to 7 days with ReLeSR reagent (STEMCELL technologies catalog #100-0484) at a ratio of 1:6 per culture ...
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bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
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bioRxiv - Bioengineering 2023Quote: IMR90-4 hiPSCs (passages 45–60) purchased from WiCell were maintained in mTeSR1 (85850, Stem Cell Technologies) in 6-well cell culture plates (30006 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged every 4-7 days using the enzyme-free passaging reagent ReLeSR (STEMCELL Technologies, 05872) which preserves colonies ...
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bioRxiv - Neuroscience 2024Quote: ... 1% minimum essential medium/non-essential amino acid (Stem Cell Technologies), 20 ng/ml brain-derived neurotrophic factor (ProSpec Bio) ...
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bioRxiv - Neuroscience 2024Quote: ... 1% minimum essential medium/non-essential amino acid (Stem Cell Technologies), 1% penicillin/streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... The media was changed daily and the iPSC cultures were split into 1:6-1:10 every 5 days using ReLeSR (Stemcell Technologies).
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bioRxiv - Genomics 2021Quote: ... tissue was viably frozen by placing 4 biopsies in 500 ml of CryoStor CS10 freezing media (STEMCELL Technologies). Tubes were inverted and then placed on ice for up to 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured for 4 days using STEMdiff™ Definitive Endoderm Differentiation Kit (Cat. No. 05110, STEMCELL Technologies) for definitive endoderm induction ...
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bioRxiv - Bioengineering 2022Quote: ... on 1:100 diluted Geltrex (Thermo Fisher)-coated plates with daily medium changes and passaging every 3-4 days with releasing agent ReLeSR (Stemcell Technologies).
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bioRxiv - Neuroscience 2024Quote: ... hESCs were fed every two days and split every 4–7 days using ReLeSR (StemCell Technologies, 100-0483) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were cultured for 4 days using STEMdiff™ Definitive Endoderm Differentiation Kit (Cat. No. 05110, STEMCELL Technologies). Media was replaced with RPMI 1640 medium ...
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bioRxiv - Developmental Biology 2023Quote: ... MEF feeders were inactivated at passage 4 with chemical treatment of 1 μg/ml mitomycin-c (STEMCELL Technologies) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C for 5 minutes and replated in a ratio of 1:6 or 1:9 on new Matrigel-coated plates in E8 supplemented with 5 μM ROCK inhibitor Y-27632 (Stemcell Technologies, Canada) for 24h ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were cultured DIV 0 to DIV 4 in Neurocult™ Neuronal Plating Medium (Stemcell Technologies, catalog no. 05713) with SM1 neuronal supplement (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended in 4 ml Red Blood Cell Lysis buffer (Ammonium Chloride solution, Stemcell Technology, #07800) on ice for 2 min and washed with 6 mL DME/F-12 medium at 450 g for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Medium was replaced every two days and cells were split every 5 to 6 days using Gentle Dissociation reagent (STEMCELL Technologies, Vancouver, Canada). hES cells were routinely tested for Mycoplasma using the Mycoplasma PCR Detection kit (Applied Biological Materials ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10,000 cells were plated per 12-well plate well onto 5% biolaminin 521 LN and differentiation started 6 hrs post plating in TeSR-E6 (Stem Cell Technologies, 05946) with CHIR99021 (Tocris ...
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bioRxiv - Developmental Biology 2020Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent and cryopreserved in mFreSR according to manufacturer’s instructions (STEMCELL Technologies). All lines were expanded and stored at low passage number.
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bioRxiv - Cell Biology 2022Quote: ... M2 polarization was achieved by stimulating MΦ cells with 20 ng/mL of interleukin 4 (IL4; Stemcell Technologies, Cat#78045) and interleukin 13 (IL13 ...
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bioRxiv - Genomics 2021Quote: ... Cells were split every 3 or 4 days with TrypLE Select (Fisher) and seeded at 1.5-4×10e5 in a Matrigel-coated p35 plate containing 5μM Y27632 (Stem Cell Technologies) (mTeSR+Y ...
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bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated overnight at 4°C with primary antibody (beta-tubulin III 1:500; STEMCELL Technologies 60052) diluted in blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... Diluted blood was overlaid carefully on SepMate-15 tubes each containing up to 4 mL Lymphoprep (STEMCELL Technologies, catalog #07851). The layered blood was centrifuged at 1,200 × g for 10 minutes at RT ...
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bioRxiv - Bioengineering 2023Quote: ... for 4 days to induce iMSCs before the medium was changed to Complete MesenCult™-ACF Plus Medium (STEMCELL Technologies) for an additional 2 days and then the cells were transferred into a new 24-well plate without an MEF feeder layer ...
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bioRxiv - Immunology 2024Quote: ... 1x108 cells/ml were incubated with 20μl/ml EasySepTM mouse Hematopoietic Progenitor Cell Isolation Cocktail (15min, 4°C, StemCell technologies), RapidSpheres (50μl/ml ...
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bioRxiv - Genomics 2021Quote: ... onto Matrigel-coated (1 mg ml-1) 6-well plates 6 using ReLeSR (STEMCELL Technologies). Cells were differentiated to CD43+ hematopoietic progenitors using the StemCell Technologies Hematopoietic Kit (Cat #05310) ...
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bioRxiv - Systems Biology 2024Quote: ... APCs were treated with the pro-inflammatory cytokines interleukin 6 protein (IL-6, Stem Cell technologies), or tumour necrosis factor alpha (TNFα ...
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bioRxiv - Genomics 2021Quote: ... Cord blood MNCs (4) were received frozen and then CD34+ selected using the EasySep human whole blood CD34 positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Cryopreserved PBMCs were quickly thawed and resuspended at a concentration of 4×106 cells/ml in Eagle's minimal essential medium (EMEM; Stem Cell Technologies; supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... CD34+ umbilical cord and CD34+ hematopoietic stem/progenitor cell (Ethics Committee Salzburg approval 415-E/1776/4-2014) were isolated using density gradient centrifugation (Lymphoprep; Stemcell Technologies). Primary human cells ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Immunology 2022Quote: HLA-A2+ monocytes were differentiated into moDCs for 7 days with GM-CSF (50 ng/ml) and IL-4 (100 ng/ml; both STEMCELL Technologies); media and cytokines were refreshed every 2–3 days ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed with basal medium (500g, 4°C) and replated in Matrigel in mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies) supplemented with Y-27632 (10 μM) ...
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bioRxiv - Neuroscience 2023Quote: hiPSC line (IMR90-4, WiCell) derived from a healthy donor and fully characterized was cultured with mTeSRTM Plus Medium (STEMCELL Technologies) on growth factor reduced matrigel-coated 6-well plates (Corning ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...