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Citations for Stemcell Technologies :
351 - 400 of 695 citations for Recombinant Mouse Cd4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse organoid culture: we used cryopreserved mouse intestinal organoids (70931, STEMCELL Technologies) derived from the C57BL/6 mouse small intestine ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant Activin-A (10 ng/ml) and 12 ng/ml FGF2 on 10μg/ml Vitronectin-XF (STEMCELL Technologies) or 0.1% Gelatin and MEF-medium coated plates ...
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bioRxiv - Genetics 2019Quote: ... CD4+ T cells fraction of the PBMCs was obtained by negative selection using EasySep® Human CD4+ T Cell Enrichment Kit (Cat. no. 19052, StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Primary CD4+ and CD8+ T cells were isolated from blood of anonymous donors by negative selection using the Human CD4+ T cell isolation kit and Human CD8+ T cell isolation kit (STEMCELL Technologies #17952 and #17953). T cells were cryopreserved in RPMI1640 (UCSF cell culture core ...
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bioRxiv - Immunology 2020Quote: CD4+ and CD8+ T cell subsets were depleted from PBMC using magnetic bead-based CD4 and CD8 negative selection kits (Stem Cell Technologies, Vancouver, Canada). The cell separations were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
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bioRxiv - Bioengineering 2022Quote: ... CD4+ T cells were isolated from the spleen of C57/BL6 mice using the CD4 T cell isolation kit from Stemcell Technologies (# 19852; Cologne, Germany) and subsequently naïve T helper cells (CD3+ ...
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bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... were differentiated into TH2 T cell subtype by incubation with specific supplement for TH2 differentiation (mouse IL-2, mouse IL-4 and rat anti mouse IFNγ with 1:100 dilution (STEMCELL Technologies)) and incubated for 3 days on plates coated with aCD3 and aCD28 antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse CD8+ T cells were isolated from the mouse spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19853A) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and mouse T cells were purified from mouse splenocytes using a negative selection EasySep™ Mouse T Cell Isolation Kit (STEMCELL Technologies). Mouse splenocytes were prepared from spleens collected from 8-18 weeks old male BL6 mice purchased from Orient bio ...
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bioRxiv - Biophysics 2021Quote: ... 05702) and media supplemented with 20ng/ml of human recombinant epithelial growth factor (EGF - STEMCELL Technologies, Cat. No. 78006). All cells were grown in a humidified incubator atmosphere at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were cultured on recombinant human VTN (rhVTN) coated 60mm plates in StemFlex media (Stem Cell Technologies, cat. A3349401) and passaged using CTS EDTA Versene Solution (Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
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bioRxiv - Genomics 2019Quote: CD4+ T cells were enriched from PBMCs by negative MACS selection using the EasySep™ Human CD4+ T Cell Isolation Kit (STEMCELL Technologies SARL, Grenoble, France), according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies, Cat#: 19157 or 19159), or isolated from purified CD4+ or CD8+ T cells by CD45RO microbeads (see Suppl ...
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bioRxiv - Immunology 2021Quote: Untouched total T or isolated CD4+ or CD8+ T cells were purified from buffy coats or whole blood with RosetteSep Human T or CD4+ or CD8+ T cell enrichment cocktails (STEMCELL Technologies, Cat#: 15061, 15062, 15063). Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Bulk CD4+ T cells were subsequently isolated from PBMCs by magnetic negative selection using an EasySep Human CD4+ T cell isolation kit (STEMCELL Technologies; per the manufacturer’s instructions). Isolated CD4+ T cells were suspended in RPMI 1640 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse neural stem cells (NSCs) were expanded in NeuroCult proliferation medium (mouse) (StemCell Technologies) supplemented with 20 ng/ml EGF ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary mouse glioma cells (CPEvIII) were cultured in NeuroCult proliferation medium (mouse) (StemCell Technologies) supplemented with 20 ng/ml EGF ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Immunology 2024Quote: ... mice and bone marrow progenitors were plated on non- tissue treated dishes and differentiated into BMDM during a 6-day culture in complete Dulbecco’s modified Eagle’s medium (DMEM) containing 5% FBS and supplemented with 60 ng/ml recombinant murine M-CSF (Stemcell Technologies) in a water saturated atmosphere at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Growth media was NeuroCult NSC Basal Medium (Mouse) containing NeuroCult Proliferation Supplement (Mouse) (StemCell Technologies), 0.2% heparin solution (Stem-Cell Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... To inhibit these pathways we used Recombinant Human Noggin Protein for BMP4 inhibition and/or XAV-939 for WNT inhibition (StemCell Technologies).
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bioRxiv - Bioengineering 2021Quote: ... and 25 ng/mL BMP4 with 50 ng/mL human recombinant Vascular Endothelial Growth Factor 165 (VEGF-165, STEMCELL Technologies, 78073) for 2 days ...
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bioRxiv - Physiology 2021Quote: ... mice fed a normal chow diet were injected with vehicle (saline or PBS), 300U/kg or 3000U/kg of recombinant human erythropoietin (EPREX, Janssen-Cilag) or 50ng/g IL-1β (STEMCELL Technologies). All animal use complied with the guidelines of the Canadian Committee for Animal Protection and was approved by IRCM Animal Care Committee.
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bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
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bioRxiv - Cancer Biology 2022Quote: ... was cultured in standard mouse NSC media containing NeuroCult basal medium (mouse & rat, STEMCELL Technologies, #05700) supplemented with NeuroCult proliferation kit (mouse & rat ...
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bioRxiv - Bioengineering 2023Quote: The Mouse IntestiCult medium (STEMCELL Technologies) was prepared and supplemented with 100 U/mL Pen/Strep (Gibco) ...
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bioRxiv - Systems Biology 2023Quote: ... Mouse splenic B cells were isolated using the EasySepTM Mouse B cell isolation Kit (STEMCELL technologies, 19854) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... B cells were isolated from mouse spleens using EasySep mouse B cell isolation kit (Stemcell Technologies, Vancouver, Canada) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: B cells were isolated from mouse spleens using EasySep Mouse B Cell Isolation Kit (Stem Cell Technologies 19854) and attached to poly-D-lysine coated coverslips at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: Mouse hepatic progenitor organoids (70932, STEMCELL Tech) were seeded in 96 well plate using 20ul Matrigel (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Nestin (1:1000; StemCell Technologies), rabbit anti-Brachyury (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Nestin (1:1000; StemCell Technologies), rabbit anti-Brachyury (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse anti-Nestin (STEMCELL Technologies; 1:100); Rabbit anti-GFAP (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg/ml mouse heparin (Stemcell technologies), 1X Pen/strept (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg/ml mouse heparin (Stemcell technologies), 1X Pen/strept (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... in mouse Mesencult Expansion medium (StemCell Technologies). After 10 days in culture ...
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bioRxiv - Biophysics 2023Quote: ... with the addition of ImmunoCult Human CD3/CD28/CD2 T Cell Activator and Human Recombinant Interleukin 2 (IL-2, STEMCELL Technologies Inc., USA) as per STEMCELL Technologies Inc ...
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bioRxiv - Cell Biology 2023Quote: ... with the addition of ImmunoCult Human CD3/CD28/CD2 T Cell Activator and Human Recombinant Interleukin 2 (IL-2, STEMCELL Technologies Inc., USA) as per STEMCELL Technologies Inc ...
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bioRxiv - Immunology 2023Quote: B cells were isolated from naïve mouse spleens with EasySepTM Mouse Pan-B cell Isolation Kit (Stem Cell Technologies) and suspended in Opti-MEM™ I Reduced Serum Medium (Gibco) ...
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bioRxiv - Immunology 2023Quote: B cells were isolated from naïve mouse spleens with EasySepTM Mouse Pan-B cell Isolation Kit (Stem Cell Technologies) and stimulated following the same method as BCR signal analyses described above ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated from naïve mouse spleens with EasySepTM Mouse Pan-B cell Isolation Kit (Stem Cell Technologies) and suspended at 1 x 106 cells/mL in the supplemented RPMI1640 (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... we performed a Cut and Run/Q-PCR (C&R/Q-PCR) experiment of 5×105 untreated cells and 5×105 cells treated for 30 minutes with recombinant human INFγ (STEMCELL Technologies, Vancouver, BC, Canada). For the C&R procedure ...
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bioRxiv - Immunology 2021Quote: Naïve CD8 T cells were isolated from young female (6w-8w) B6 mouse lymph nodes and spleens with the EasySep Mouse Naïve CD8 T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol ...