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Citations for Stemcell Technologies :
251 - 300 of 695 citations for Recombinant Mouse Cd4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 20 ng/ml recombinant human EGF (STEMCELL Technologies, cat# 78006). For passaging ...
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bioRxiv - Bioengineering 2023Quote: ... with the addition of Human Recombinant Interleukin 2 (STEMCELL Technologies, Cat# 78036.3). To prepare 3 mg/ml collagen-I gel ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells were purified from CD45.1+ CD45.2+ or CD45.2+ SMARTA splenocytes by negative selection (STEMCELL Technologies) and stimulated in 12-well plates pre-coated with 8 μg/mL anti-CD3 (145-2C11 ...
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bioRxiv - Immunology 2020Quote: Naïve CD4+ T cells were negatively isolated from fresh PBMCs by EasySep (Stem Cell Technologies; >95% purity). Cells were stimulated (Ellis et al. ...
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bioRxiv - Genomics 2021Quote: ... Frozen vials of primary human CD4+CD45RO+ memory T cells were purchased from STEMCELL Technologies (Cat #70031).
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bioRxiv - Immunology 2020Quote: ... For Il2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Genomics 2023Quote: Primary CD25-CD4+ effector T cells were isolated from fresh Human Peripheral Blood Leukopaks (STEMCELL Technologies, #70500) from healthy donors ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with human recombinant epidermal growth factor (20ng/mL: STEMCELL Technologies, Cat#78006), basic fibroblast growth factor (20ng/mL ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 ng/mL Recombinant Human Epidermal Growth Factor (EGF) (StemCell Technologies, Cat 02633), 10 ng/mL Recombinant Human Basic Fibroblast Growth Factor (Bfgf ...
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bioRxiv - Bioengineering 2021Quote: ... and 100ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1) in RPMI 1640 for 2 days ...
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bioRxiv - Bioengineering 2021Quote: ... and 10 ng/mL human recombinant Epidermal Growth Factor (EGF, STEMCELL Technologies, 78006.1), and passaged using TrypLE™ Express Enzyme.
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bioRxiv - Bioengineering 2021Quote: ... and 10 ng/mL human recombinant Epidermal Growth Factor (EGF, STEMCELL Technologies, 78006.1), and passaged using TrypLE™ Express Enzyme.
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bioRxiv - Bioengineering 2021Quote: ... and 100ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1) for 2 days ...
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bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1), 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with Human Recombinant IL-2 (12.5 ng/mL) (Stemcell Technologies, Cat.#78036), with initial seeding at 1×106 alive cells/mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with Human Recombinant IL-2 (12.5 ng/mL) (Stemcell Technologies, Cat.#78036), IL-7 (5 ng/mL ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10 ng/mL of Human Recombinant IL-2 (STEMCELL TECHNOLOGIES, #78220) and ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL TECHNOLOGIES ...
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bioRxiv - Neuroscience 2024Quote: ... human recombinant shh (C24II) (cat# 78065.1) (all from STEMCELL Technologies, Tullamarine, VIC, Australia). See table S2 for antibodies used for ICC.
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bioRxiv - Cell Biology 2020Quote: ... Naïve CD4 T cells were isolated from CB mononuclear cells (CBMCs) obtained using Lymphoprep (StemCell Technologies Inc., Canada), followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells and CD19+ B cells were negatively isolated using magnetic bead based separation (EasySep, StemCell Technologies). CD14+ monocytes were positively selected (EasySep ...
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bioRxiv - Bioengineering 2020Quote: ... as per the manufacturer’s instructions and were enriched for CD4+ or CD8+ T cells using RosetteSep (STEMCELL Technologies) T cell enrichment as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... counted and CD8+ or CD4+ T cells were magnetically separated with appropriate separation kits (Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Immunology 2020Quote: ... prior to CD4 and CD8 T cells isolation using EasySep Human T cell isolation kit (STEMCELL Technology, #17951). Isolated T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... CD14+ monocytes and CD4+ T-cells were purified from PBMCs by negative magnetic selection (Stemcell Technologies, Grenoble, France). Monocytes (1×106/well ...
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bioRxiv - Immunology 2020Quote: ... EasySep human CD4+ T cell enrichment kit and EasySep human CD8+ T cell enrichment kit (STEMCELL Technologies, Canada), respectively ...
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bioRxiv - Immunology 2023Quote: ... For Il-2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Molecular Biology 2023Quote: ... starting from freshly isolated PBMCs from 69 PLWH using the EasySep CD4 T cell isolation kit (Stemcell Technologies). CD4+ T cells were frozen as dry pellet at −80°C prior to DNA extraction with the DNeasy Blood & Tissue kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... naive Treg cells were purified by using EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies). For human cell culture ...
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bioRxiv - Microbiology 2024Quote: Primary human CD4+ T cells from healthy donors were isolated from leukoreduction chambers after Trima apheresis (STEMCELL Technologies). PBMCs were isolated by Ficoll centrifugation ...
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bioRxiv - Immunology 2021Quote: ... Murine CD4 T cells were isolated from processed lymphoid organs using EasyEights magnet (Stem Cell Technologies, Catalog no. 18103) and the whole CD4+ T cell isolation kit (Stem Cell Technologies ...
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bioRxiv - Microbiology 2020Quote: ... 5×106 naïve CD4+ T cells from HIV− or HIV+ donors were isolated by magnetic negative selection (StemCell Technologies), then cultured at 106 cells/mL in 96-well plates using R-10 media (RPMI-1640 media supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... Enriched CD4+ and CD8+ T cells were activated with ImmunoCult NHP CD2/CD3/CD28 T-cell activator (STEMCELL Technologies) and incubated 3 days at 37°C in humidified 5% CO2.
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bioRxiv - Immunology 2020Quote: CD4 or CD8 T cells were isolated from spleen using magnetic beads as described by manufacturer’s protocol (STEMCELL technologies). 4000 cells in ratio 1:1 were transferred into WT mice 1 day prior to LCMV-Cl13 infection.
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bioRxiv - Immunology 2021Quote: ... and CD4+ T cells were pre-activated with either CD3/CD28/CD2 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies) or 1:1 aAPCs for 5 d ...
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bioRxiv - Immunology 2020Quote: ... we treated CD4+ T cells with either αCD3αCD28 (ImmunoCult™ Human CD3/CD28 T Cell Activator; STEMCELL Technologies, 10971) or IFN I (1:1000 ...
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bioRxiv - Microbiology 2023Quote: Phosphorylation status of p65 and c-JUN were analyzed by flow cytometry in isolated CD4 T cells (StemCell Technologies) as previously described (44) ...
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bioRxiv - Biophysics 2023Quote: Primary human CD4+ T cells were isolated from commercially available whole human blood (STEMCELL Technologies Inc., USA, Cat# 70507.1) with EasySep Human CD4+ T Cell Isolation Kits (STEMCELL Technologies Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... and Naïve CD4+ T cell were isolated from splenocytes with commercial kits (STEMCELL Technologies, Cat No. 19852, 19853, 19765) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... CD4+ T cells were activated on-chip by adding ImmunoCult™ Human CD3/CD28 T Cell Activator (StemCell Technologies) to the culture media ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 ng/mL Recombinant Human Basic Fibroblast Growth Factor (Bfgf) (StemCell Technologies, Cat 02634), 2 μg/mL Heparin (StemCell Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... and 40 ng/mL animal component-free human recombinant Activin A (STEMCELL Technologies, 78132) for 12 days with media changes every other day ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 30 ng/mL human recombinant epidermal growth factor (rhEGF) (STEMCELL Technologies 78006) and filtered through a 40 μm filter (Fisherbrand™ 22-363-547 ...
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bioRxiv - Biophysics 2023Quote: ... with the addition of Human Recombinant Interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... and T-cells were isolated via the Easy-Sep™ Rosette Human CD4+ T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2019Quote: T cells were purified from normal donors by negative selection using the RosetteSep Human CD4+ T Cell Enrichment Cocktails according to the manufacturer’s protocols (StemCell Technologies). Primary cells were activated and expanded using Human T-activator CD3/CD28 Dynabeads (Cat # 11161D ...
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bioRxiv - Synthetic Biology 2020Quote: ... CD4+ and CD8+ T cells were isolated using the RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies, 15022 and 15023), and PBMCs were isolated using Lymphoprep (STEMCELL Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies) and were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Immunology 2020Quote: Total CD4 T cells from donor mice were purified from spleens using STEMCELL EasySep CD4 T Cell Isolation Kit according to manufacturer’s instruction (STEMCELL Technologies). Depending on individual experiment ...
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bioRxiv - Immunology 2022Quote: ... Mature CD4 single positive (SP) thymocytes were negatively enriched using the EasySep Biotin Positive Selection Kit for depletion (STEMCELL Technologies) as previously described (51) ...
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bioRxiv - Immunology 2022Quote: ... lymph nodes and spleen from 16-24-week-old female or male C57Bl/6-Foxp3gfp × Thy1.1 mice were collected and CD4+ T cells isolated by negative selection (STEMCELL Technologies). Tregs were sorted as live CD4+CD8-Thy1.1+Foxp3gfp+ using a MoFlow® Astrios (Beckman Coulter ...