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Citations for Stemcell Technologies :
301 - 350 of 353 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... PBMC and plasma samples were isolated after whole blood centrifugation 1200 g for 10 minutes at room temperature in SepMate tubes (STEMCELL Technologies). Plasma was stored at −80°C until analysis ...
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bioRxiv - Neuroscience 2021Quote: ... centrifuged at 200 x g for 5 min prior to be resuspended in STEMdiff™ Neural Progenitor Medium (Stem Cell Technologies) and seeded onto growth-factor-reduced Matrigel™-coated plates (8.6µg/cm2 ...
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bioRxiv - Cell Biology 2020Quote: ... The dissociated glands were spun down at 450 x g for 5 min and resuspended in ammonium chloride solution (STEMCELL Technologies) diluted (4:1 ...
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bioRxiv - Physiology 2021Quote: ... mice fed a normal chow diet were injected with vehicle (saline or PBS), 300U/kg or 3000U/kg of recombinant human erythropoietin (EPREX, Janssen-Cilag) or 50ng/g IL-1β (STEMCELL Technologies). All animal use complied with the guidelines of the Canadian Committee for Animal Protection and was approved by IRCM Animal Care Committee.
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bioRxiv - Molecular Biology 2020Quote: ... frozen cord blood and Plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells, Alameda, CA, USA and STEMCELL Technologies, Vancouver, Canada), frozen Plerixafor- and/or G-CSF-mobilized peripheral blood of patients with SCD ...
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bioRxiv - Immunology 2021Quote: ... PBMC and plasma samples were isolated after whole blood centrifugation 1200 g for 10 minutes at room temperature in SepMate tubes (STEMCELL Technologies). Plasma was stored at -800C until analysis ...
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bioRxiv - Bioengineering 2024Quote: ... M2-10B4 (overexpressing IL-3 and G-CSF) and Sl/Sl (overexpressing IL-3 and SCF) were passaged at ∼90% confluence (cell lines gifted from StemCell Technologies). Cells were grown for 2 weeks prior to use in selection agents to select stromal cells expressing long-term cell maintenance factors (M2-10B4 ...
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bioRxiv - Molecular Biology 2024Quote: CD34+ HSPCs were isolated from umbilical cord blood (provided by Stanford Binns Program) or sourced from Plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells and STEMCELL Technologies). Bone marrow aspirates taken from patients with αTM under protocol no ...
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bioRxiv - Cancer Biology 2020Quote: Feeder-free H1 hESCs (WiCell) were cultured on Matrigel in TeSR™-E8™ kit for hESC/hiPSC maintenance (StemCell Tech; #05990). hESCs were used to generate COs using media included in the STEMdiff Cerebral Organoid Kit (StemCell Tech ...
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice a week using an enzyme-free dissociation reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Genomics 2021Quote: ... were sorted using a BD FACSAria™ Fusion Cell Sorter into ice-cold Serum-Free Expansion Medium (SFEM) supplemented with 100 ng/ml SCF and Flt3 (Stem Cell Technologies), 20 ng/ml IL-3 ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed cells were cultured at 37°C and 5%O2 in serum-free HSC media comprised of StemSpan II medium (Stem Cell Technologies) supplemented with CC100 cytokine cocktail (Stem Cell Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies). For autophagy induction cells were treated with 200 µM Rapamycin (ChemCruz ...
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bioRxiv - Neuroscience 2020Quote: ... gene were cultured according to Stem Cell Technologies protocols that can be found in (https://www.stemcell.com/maintenance-of-human-pluripotent-stem-cells-in-mtesr1.html) on feeder free in hESCs medium on Matrigel (StemCell Technologies, Cat. #354277) in mTeSR (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... NK cells were allowed to recover at a density of 1.5 × 106 cells/mL in antibiotic-free medium containing 1 ug/mL DNase I solution (STEMCELL Technologies, Cambridge, MA), and were then cultured in complete NK cell medium at a density of 6 × 105 cells/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was refreshed every 2-3 days and hiPSCs were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Developmental Biology 2021Quote: Culture of isolated human CB-derived CD34+ cells was performed in HSPC expansion media comprised of Stem Span serum-free media (StemCell Technologies #09855) supplemented with Glutamax (Invitrogen #35050061) ...
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bioRxiv - Developmental Biology 2022Quote: KOLF2 iPS cells were grown under feeder-free conditions on Matrigel-coated plates in TeSR™-E8™ (Stem Cell Technologies, #05990).
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bioRxiv - Neuroscience 2022Quote: ... the iPSCs were seeded in 6-well plates coated in phenol-red free Matrigel with hematopoietic differentiation media (StemCell Technologies, cat.no. 05310). The media was changed following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Lin- cells were transduced overnight at a viral multiplicity of infection (MOI) of 0.3-50 (Table S6) in StemSpan Serum Free Expansion Medium (SFEM) culture medium (StemCell Technologies, Vancouver, Canada) supplemented with 50 ng/mL recombinant murine stem cell factor (SCF) ...
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bioRxiv - Genetics 2024Quote: ... CD34+ HSPCs were cultured at 1×105–5×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies, Vancouver, Canada) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM ...
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bioRxiv - Neuroscience 2023Quote: ... were cultured according to Stem Cell Technologies protocols that can be found in (https://www.stemcell.com/maintenance-of-human-pluripotent-stem-cells-in-mtesr1.html) on feeder free in hESCs medium on Matrigel (StemCell Technologies, Cat. #354277) in mTeSR (Stem Cell Technologies ...
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bioRxiv - Genomics 2023Quote: ... directly into ice-cold complete medium (Stemspan serum-free expansion medium (SFEM) supplemented with 100 ng/ml SCF and Flt3 (Stem Cell Technologies) and 20 ng/ml IL-3 ...
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bioRxiv - Bioengineering 2023Quote: ... Medium was refreshed every 2–3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... H9 human embryonic stem cells (Agreement No. 19-W0538; WiCell, USA) were maintained as bulk culture in feeder-free conditions on vitronectin (StemCell Technologies, Canada)-coated dish in TeSR-E8 basal medium plus supplement (StemCell Technologies ...
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bioRxiv - Bioengineering 2023Quote: The human embryonic stem cell (hESC) line ‘Man13’ were cultured as colonies under feeder-free conditions in TeSR-E8 culture medium (StemCell Tech 05990), on 6-well tissue culture plates pre-coated with VTN (GIBCO 14700)1 ...
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bioRxiv - Cell Biology 2023Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Bioengineering 2024Quote: hPSCs were maintained on Geltrex-coated plates in feeder-free mTeSR-1TM and mTeSR PlusTM media (Stemcell Technologies #85850 and #100-0276) using an established protocol (63) ...
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bioRxiv - Bioengineering 2024Quote: ... or modified Caf1 cell culture scaffolds coated 60 mm culture dishes with mTeSR™ Plus stabilised feeder-free maintenance medium (100-0276, STEMCELL Technologies) following the manufacturers’ recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were centrifuged and resuspended in serum-free medium composed of IMDM with GlutaMAX and 20% BIT9500 (Stem Cell Technologies, 09500) supplemented with three cytokines involved in stem cell maintenance ...
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bioRxiv - Cell Biology 2024Quote: ... Human iPSCs and H9 human embryonic stem cells (hESCs) were maintained on a feeder-free system using Matrigel coated culture plates and TeSR-E8 media Kits (STEMCELL Technology Inc.).27 All culture media and reagents were purchased from Invitrogen (Carlsbad ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2, and 10 µg ml-1 Deoxyribonuclease I, Stem Cell Technologies, 07900), and mixed 70 times with a 1000 µl pipette tip ...
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bioRxiv - Cancer Biology 2024Quote: ... To obtain single cells the tissue was centrifuged at 450 x g for 3 min and the cell pellet was resuspended in 1 mL Dispase 5 U (Stemcell Technologies, 07913) with 100 μL of DNase I 1 mg/mL (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with DMEM (centrifuged at 300 g for 10 mins at 4°C) and further dissociated into single cells using the NeuroCult Chemical Dissociation Kit (STEMCELL Technologies; #05707) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The transfected LCLs were then plated into a matrigel-coated six-well plate and maintained in TeSr-E7 xeno-free containing media (Stem Cell Tech Inc.). Twelve days after transfection ...
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bioRxiv - Bioengineering 2021Quote: ... Matrigel was used coat culture dishes for feeder-free expansion of induced pluripotent stem cells and were routinely cultured and maintained in mTeSR™1 (STEMCELL Technologies, 85850) in a humidified incubator at 37 °C with 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured in serum-free conditions in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies; Cat# 05750 and #05753) supplemented with 10 ng/ml basic fibroblast growth factor and 20 ng/ml human epidermal growth factor.
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bioRxiv - Genomics 2020Quote: ... Cells were passaged every four to six days as clumps using ReLeSR™ an enzyme-free reagent for dissociation (StemCell Technologies, Cat.: #05872) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from tumor-free wild type B6 mice using the mouse CD8 T cell isolation kit (STEMCELL Technologies cat #19853), labeled with Cell Trace Violet (Thermo Fisher cat #C34557 ...
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bioRxiv - Immunology 2024Quote: ... and mesenteric) from mice were sterilely collected in iron free media and homogenised through 40 μm filters using EasySep buffer (Stem Cell Technologies, 20144) or an in-house alternative (PBS + 2% FBS + 1mM EDTA (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... NK cells were stained with CellTrackerTM Green CMFDA (5-chloromethylfluorescein diacetate) (InvitrogenTM C7025) diluted to 10 µM in serum free ImmunoCultTM NK Cell Base Medium (STEMCELL Technologies 100-0712) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... we used human WTC hiPSCs (a gift from Professor Bruce Conklin) cultured on feeder- free system on Matrigel in mTeSR medium (STEMCELL Technologies, Vancouver, Canada). We initiated the organoid development by plating hiPSCs at density 20- 30% in TeSR1 medium on a 6-well plate coated with human embryonic stem cell (hESC)-qualified basement membrane matrix (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were pelleted at 350x g for 2 min and transferred onto a 6-well transwell membrane (Stem Cell Technologies, #3450). Pellets were incubated with CHIR99021 (5 µM ...
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bioRxiv - Neuroscience 2020Quote: ... The pellet was centrifuged again at 200 g for 12 minutes and then dissociated with a 200μl pipette and plated in Neurocult proliferation medium (Stem cell Technology, BC, CA) supplemented with EGF (20 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood using density gradient centrifugation at 1,200 x g for 10 min where diluted blood was layered over 15 mL of Lymphoprep™ (STEMCELL Technologies, Vancouver, Canada) followed by harvesting of the buffy coat ...
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bioRxiv - Genetics 2024Quote: ... CD34+ HSPCs were isolated from cord blood (provided by Stanford Binns Program for Cord Blood Research) or sourced from Plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells, Alameda, CA, USA and STEMCELL Technologies, Vancouver, Canada). Frozen Plerixafor- and/or G-CSF-mobilized peripheral blood of patients with sickle cell disease were provided by Dr ...
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bioRxiv - Synthetic Biology 2024Quote: ... CD34+ HSPCs were sourced from fresh cord blood (generously provided by the Stanford Binns Family Program for Cord Blood Research) and Plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells, Alameda, CA, USA or STEMCELL Technologies, Vancouver, Canada). CD34+ HSPCs were cultured at 1-5×105 cells/mL in StemSpan SFEMII (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...
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bioRxiv - Immunology 2022Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×106 T cells/ml in serum-free StemCell Immunocult™-XF media (Cat#10981, StemCell Technologies, Vancouver, Canada) with 20U/ml clinical grade human IL-2 (Novartis) ...
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bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich], and 10 uM Y27632 [72304, StemCell Technologies] in mTeSR1), and centrifuged at 3200 xg for 30 to 90 min ...