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Citations for Stemcell Technologies :
251 - 300 of 353 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... We grew cells in a 5% CO2 incubator in a serum-free NeuroCult NS-A basal medium (STEMCELL Technologies) supplemented with NeuroCult™ Proliferation Supplement (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2) at a cell concentration of 5 × 105/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies) supplemented with 100 ng/mL of human recombinant SCF ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR; Stem Cell Technologies, 05872). Cells were checked for mycoplasma contamination every two weeks ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were plated immediately into pre-warmed antibiotic-free HSPC media (StemSpan SFEM II; Stemcell Technologies; cat. no., 09605_C) supplemented with SCF (100 ng/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Biochemistry 2024Quote: ... HEL cells (originally sourced from ATCC) were then stably transduced with the resulting lentivirus and successful transductants selected using puromycin (1.25 µg/mL; StemCell Technologies) as before46 ...
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bioRxiv - Cell Biology 2024Quote: ... Low-density mononuclear cells were isolated through centrifugation with a Lymphoprep density gradient (density = 1.077 ± 0.001 g/cm2) (STEMCELL Technologies). hBMSCs were selected through the process of plastic adherence ...
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bioRxiv - Bioengineering 2021Quote: ... the cuvettes were allowed to rest for 15 minutes before the T cells were gently moved to antibiotic-free medium containing 1µg/mL DNase (StemCell Tech) at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
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bioRxiv - Neuroscience 2022Quote: iPSCs were cultured in feeder-free conditions on Matrigel-coated (0.08 mg/well) 6-well tissue culture plates in mTesR1 media (StemCell Technologies) at 37 °C and 5 % CO2 ...
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bioRxiv - Genomics 2023Quote: Unedited and WDR90 knock-out iPSC clones were transitioned into feeder-free condition in mTeSR™ Plus medium (STEMCELL Technologies) in a matrigel-coated 6 well plate at a density of 5x105 cells/well with 5 µM ROCK Inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... CS-001) were cultured on Matrigel-coated plates in feeder-free conditions in mTeSR-1 medium (Stemcell Technologies, Vancouver, Canada). As schematized in Fig ...
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bioRxiv - Genomics 2024Quote: ... For iPS adaptation to feeder-free culture colonies were seeded on Matrigel-coated plates in mTeSR Plus medium (STEMCELL Technologies) and passaged at least three times until no feeder cells were observed ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were centrifuged at 200 x g for 2 min and resuspended in neural media (NM; Supplementary Table 2) supplemented with 0.02 µg/mL FGF2 (StemCell Technologies, #78003) and 0.02 µg/mL EGF (StemCell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... For immunolabelling embryos were washed 4 times with PTwH (PBS/0.2% Triton-X-100/10 µg/mL heparin (Stemcell Technologies, #07980)) for 1 h each at 37°C and incubated with primary antibody (PE-labelled rabbit anti-CD31/PCAM1 (1:100 ...
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bioRxiv - Genetics 2024Quote: ... Contractile VSMCs were then induced with N2B27 supplemented with 2 ng/ml Activin A and 2 µg/ml Heparin (STEMCELL Technologies) for 5 days ...
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bioRxiv - Genomics 2024Quote: ... Contractile VSMCs were subsequently induced by culturing in N2B27 medium containing 2 ng/ml Activin A and 2 µg/ml Heparin (STEMCELL Technologies) for 6 days ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... centrifuged at 50 x g for 5 min and subsequently dissociated by incubation with gentle cell dissociation solution (StemCell Technologies) for 15 min and additional T/E for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were spun down (120 × g) for 3 min and resuspended in the required amount of mTeSR (Stem Cell Technologies) + Rock Inhibitor (RI) ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged (350 x g for 5 minutes) and resuspended in 1 mL of prewarmed dispase (#07913, Stemcell Technologies) and 100 μL of DNase I (#07900 ...
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bioRxiv - Genomics 2021Quote: ... cells were centrifuged (350 x g for 5 minutes) and resuspended in 5 mL of prewarmed 0.25% trypsin/EDTA (#07901, Stemcell Technologies) at 37°C for 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... The preparation was centrifuged (200 g; 7 min) and the pellet resuspended in PneumaCult-Ex Plus expansion medium (Stemcell Technologies), then seeded onto 25 cm2 tissue culture flasks pre-coated with 30 µg/mL Type I collagen (PureCol ...
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bioRxiv - Immunology 2023Quote: ... uncoagulated whole blood from male and female donors (age range: 21-66) was spun at 1,200 × g in 50-mL SepMate tubes (STEMCELL Technologies) containing Ficoll-Paque PLUS (Cytiva ...
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bioRxiv - Immunology 2024Quote: PBMCs were isolated from blood of BCG-vaccinated and non-vaccinated volunteers using gradient centrifugation with Lymphoprep (density 1.077 g/mL; STEMCELL Technologies). Monocytes were enriched with CD14+ microbeads (MACS ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... between 50-250*103 cells per 200µl of the following culture media: Human LT-HSCs (LIN−/CD38lo/CD34+/CD45RA−/CD90+) in StemSpan Serum-free expansion medium (SFEM; Stemcell Technologies) supplemented with 100ng/ml human SCF ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were cut into 1-2 mm pieces and subsequently incubated in serum-free medium containing collagenase/hyaluronidase (Stem Cell Technologies) at 37 ºC ...
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bioRxiv - Cancer Biology 2021Quote: ... CD34+ cells from MF patients were isolated as described above and cultured in StemSpan serum free medium (SFEM; cat# 09605, StemCell Technologies) with stem cell factor (SCF ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary patient material was aliquoted into 24- or 48-well plates in 200μl StemSpan™ Serum-Free Expansion Medium (SFEM) (STEMCELL Technologies) supplemented with 20% FCS and the following cytokines ...
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bioRxiv - Cancer Biology 2022Quote: CD34-positive and Lin-negative cells from PBMC were single-cell sorted into 96-well plates containing 100 μL of free medium consisting of StemSpam SFEMII (Stemcell Technologies) with human cytokines SCF ...
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bioRxiv - Genetics 2022Quote: ... HSPCs were transferred into erythroid differentiation medium (EDM) consisting of StemSpan serum-free expansion medium II(SFEM II; Stem Cell Technologies) with 1% penicillin/streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... The CHOP-WT10 cell line was maintained as an iPSC culture35 and subsequently transitioned to feeder-free cultures and maintained with mTeSR1 (StemCell Technologies) on hESC qualified matrigel (Corning) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured in feeder-free medium and grown on Matrigel-coated plates in cell medium containing 1:1 mTesR1: Essential 8 (StemCell Technologies, 85850 and Thermofisher A1517001 ...
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bioRxiv - Cell Biology 2024Quote: ... Semi-solid MC3434 cell mixtures were plated at 1 mL mixture/well in a 6-well meniscus-free dish (SmartDishTM, STEMCELL Technology) using a 3cc syringe and a blunt-end 16-gauge needle (STEMCELL Technology) ...
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bioRxiv - Cell Biology 2023Quote: For cell growth assay 100 LIN-CD34+CD38- CD9+ or CD9- cells were sorted and seeded in 96 well plate and culture in StemSpan Serum –Free Expansion Medium (SFEM, StemCell Technologies) supplemented with 100u/ml penicillin (Hyclone ...
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bioRxiv - Neuroscience 2024Quote: ... in a feeder free fashion and passaged every 4∼5 days at a 1:4∼1:6 ratio using ReLeSR (Stemcell technologies) as described previously 88,95 ...
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bioRxiv - Developmental Biology 2024Quote: ... SSI and SSII media are parts of a 2-step suspension culture system with a base medium consisting of StemSpan Serum-Free Expansion Medium II (StemCell technologies), 2 mM of L-glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 104 sorted cells were plated in triplicate in serum-free media containing StemSpan™ Megakaryocyte Expansion Supplement (STEMCELL Technologies) for 10-12 days ...
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bioRxiv - Genomics 2023Quote: ... The iPSCs were transitioned into feeder-free conditions inhouse and maintained as feeder-free cultures in mTeSR Plus basal medium supplemented with mTeSR Plus 5X supplement (StemCell Technologies). The cultures were grown in multiwell plates coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then passaged using GCDR and replated on 10-cm dishes coated with Animal Component Free (ACF) Attachment Substrate (StemCell Technologies); all subsequent passages were grown using the same medium and culture substrate ...
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bioRxiv - Cell Biology 2022Quote: ... were obtained from the CCHMC Pluripotent Stem Cell Facility (PSCF) (44) and cultured in feeder-free condition with mTeSR1 (85850, StemCell Technologies) and on a hESC-qualified Matrigel (354277 ...
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bioRxiv - Genomics 2023Quote: ... hESC was maintained and expanded in feeder-free culture conditions with mTeSR basal medium (STEMCELL Technologies Inc., Vancouver, Canada, cat # 85850) on 6-well plates culture-treated coated with Corning Matrigel hESC-qualified Matrix ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed and resuspended in a fresh pre-warmed complete culture medium and cultured in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ cells were seeded at the concentration of 5*105 CD34+ cells/ml in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 106 lineage negative cells/ml were transduced with 1 x 108 TU/ml of empty vector or Vegfc lentivirus in serum-free Stem-Span medium (StemCell Technologies) supplemented with 10 ng/ml IL-3 ...
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bioRxiv - Immunology 2024Quote: ... The purified cells (purity determined as mentioned below) were then plated on Animal-Component-Free coated cell culture plates using Endothelial Expansion Media (StemCell Technologies) for expansion ...
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bioRxiv - Bioengineering 2024Quote: Our protocol began with cultivating human pluripotent stem cells (H9) in a Matrigel-coated 6-well plate using a stem cell feeder-free culture medium (mTeSR1, STEMCELL Technologies). For routine passaging ...
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bioRxiv - Cancer Biology 2024Quote: Control and ammonia-treated hepatospheres were dissociated into single cells using an enzyme-free dissociation reagent (StemCell Technologies, Cat. 100-0485), counted ...
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bioRxiv - Genomics 2024Quote: ... 4.5–9 x 104 cells were plated onto 12-well plates pre-coated with 80 µg/µl growth factor- reduced Matrigel in mTeSR1 (StemCell Technologies; 85850) and 10 µM Y-27632 ...
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bioRxiv - Bioengineering 2024Quote: ... Purified cell populations were expanded in endothelium maintenance media consisting of conditioned media diluted at 1:1 ratio with StemPro-34 SFM supplemented with 2 µg/mL heparin (STEMCELL Technologies, 07980). Media was replaced every other day or until the conditioned media was depleted ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...