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Citations for Stemcell Technologies :
301 - 350 of 569 citations for IL 4 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Intestinal crypts were dissociated from mouse small intestine using Gentle Cell Dissociation Reagent (Stemcell Technologies, Cambridge, UK). The crypts were then re-suspended in Intesticult medium (Stemcell Technologies ...
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bioRxiv - Microbiology 2019Quote: Pretumor splenic B cells were purified using the Mouse Pan-B Cell Isolation Kit by StemCell Technologies. Bone marrow cells were flushed from femurs and tibia ...
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bioRxiv - Immunology 2020Quote: ... were purified via negative selection (EasySep™ Mouse CD4+ T cell Isolation Kit, STEMCELL Technologies, Lot: 18H94653). Purified CD4+ T cells were cultured for 72 hours with interleukin-12 (IL-12 ...
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bioRxiv - Immunology 2019Quote: ... Human CD45+ cells were enriched using the EasySep™ Mouse/Human Chimera Isolation Kit (Stemcell technologies, 19849) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and CD8+ T cells were negatively selected using EasySep mouse CD8+ T cells isolation kit (StemCell Technologies), following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Resting B cells were isolated using the EasySep Negative Selection- Mouse B Cell Isolation Kit (STEMCELL Technologies), which depletes for non-B cell markers and activated CD43+ B cells ...
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bioRxiv - Immunology 2022Quote: ... and incubated with NK cells isolated using the mouse NK cell isolation kit (STEMCELL Technologies cat #19855) from tumor-free wild type B6 mice in the indicated ratios in the presence of IL-2 as described previously (72) ...
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bioRxiv - Immunology 2022Quote: Bone-marrow eosinophils were isolated using EasySep™ Mouse Biotin Positive Selection Kit II (Stem Cell Technologies) according to manufacturer instructions ...
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bioRxiv - Physiology 2022Quote: Mouse jejunal section were used to isolate crypts according to the manufacturer’s instructions (STEMCELL Technologies, document 28223). Complete IntestiCult medium was exchanged every 2-3 days and organoid numbers and de novo crypts were scored on days 5 and 7 ...
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bioRxiv - Cancer Biology 2023Quote: ... All sorted cells were grown in Mouse MesenCult Expansion Kit Media (05513; StemCell Technologies, Vancouver, BC, Canada). Cells were cultured at 37 °C with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The mouse MLL-AF9-transduced cells were cultured in MethoCult™ M3234 (STEMCELL Technologies, Bancouber, BC, Canada). The human MLL-AF9-transduced cells were cultured in StemSpan SFEM II medium (#ST-09655 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse intestinal organoids were cultured in 24-well plates using IntestiCult organoid growth medium (06005, STEMCELL Technologies). In short ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were cultured for 7 d at 5% O2 in EpiCult- B mouse medium (STEMCELL Technologies, 05610) supplemented with 5% FBS ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes or BM cells were enriched using EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Stemcell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Mouse myeloid cells were differentiated in IMDM (Cytiva, SH30228.FS) supplemented with 20% BIT9500 (StemCell Tech, 09500), 20 ng/mL M-CSF (Peprotech ...
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bioRxiv - Genomics 2020Quote: ... H1 cells were daily fed with fresh mTeSR1 media and passaged every 4-5 days using ReLeSR reagent (StemCell Technologies, 05872). Cells were dissociated into single cells with TrypLE Express (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... cells were maintained on 6-well plates in a humidified incubator at 37°C with 5% CO2 and passaged every 3-4 days (at ∼80% confluence) using ReLeSR (Stemcell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... H9-hESC SOX10::GFP reporter and H20961 iPSC were passaged ∼1:6 every 4-5 days by incubating them in ReLeSR (StemCell technologies) for 1 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... Cord blood MNCs (4) were received frozen and then CD34+ selected using the EasySep human whole blood CD34 positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Cryopreserved PBMCs were quickly thawed and resuspended at a concentration of 4×106 cells/ml in Eagle's minimal essential medium (EMEM; Stem Cell Technologies; supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells where count and seeded at a density of 2×10^4 cells per replicate into cytokine-supplemented methylcellulose medium (MethoCult M3434, Stemcell Technologies). After 10-14 days the colonies propagated and scored ...
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bioRxiv - Developmental Biology 2022Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent (GCDR, STEMCELL Technologies). Cells were cryopreserved in mFreSR.
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bioRxiv - Developmental Biology 2022Quote: ... they were specified into a hiPSC-CM/stromal cell mixture using RPMI B27-containing 5 µM IWP-4 (Stem Cell Technologies) followed by another 7 days of RPMI B27+ (RPMI1640 GlutaMAX + 2% B27 supplement with insulin ...
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bioRxiv - Neuroscience 2021Quote: ... were generated from confluent iPSC cultures by dissociating them to single cells and seeding them at a density of ∼4 × 106 cells per well of Aggrewell 800 6-well plates (StemCell Technologies), equating to ∼2200 cells per EB ...
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bioRxiv - Cancer Biology 2021Quote: IL-10 polarized human DCs (3×10^4/well) were incubated with allogeneic CD8+ T cells (1.5 × 10^5/well) in T cell media (Stem Cell Technologies) in 96 well flat bottom tissue culture plates for 4 days ...
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bioRxiv - Immunology 2021Quote: ... CD34+ umbilical cord and CD34+ hematopoietic stem/progenitor cell (Ethics Committee Salzburg approval 415-E/1776/4-2014) were isolated using density gradient centrifugation (Lymphoprep; Stemcell Technologies). Primary human cells ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Cell Biology 2023Quote: ... For immunolabelling embryos were washed 4 times with PTwH (PBS/0.2% Triton-X-100/10 µg/mL heparin (Stemcell Technologies, #07980)) for 1 h each at 37°C and incubated with primary antibody (PE-labelled rabbit anti-CD31/PCAM1 (1:100 ...
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bioRxiv - Neuroscience 2023Quote: hiPSC line (IMR90-4, WiCell) derived from a healthy donor and fully characterized was cultured with mTeSRTM Plus Medium (STEMCELL Technologies) on growth factor reduced matrigel-coated 6-well plates (Corning ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...
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bioRxiv - Neuroscience 2023Quote: ... iPSC cultures were passaged every 4-5 days when the colonies reached 80% confluency using the dissociation reagent ReLeSR (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... hiPSCs were seeded onto Matrigel-coated 6-well tissue culture treated plates and maintained for 4 days with mTeSR1 media (85850, Stemcell Technologies). On day 0 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were transferred into TeSR-E7 medium and maintained until the emergence of iPSC colonies (about 4 weeks) which were then manually isolated and propagated in mTeSR medium (StemCell Technologies). Absence of plasmid integration and clearance of Sendai virus were confirmed ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were isolated from bone marrow (femurs and tibias) with the EasySepTM Mouse Monocyte Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... B cells were purified from a B6 mouse using an EasySep B cell negative selection kit (StemCell Technologies) and labelled with CFSE before being transferred at 4e6 per mouse ...
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bioRxiv - Immunology 2021Quote: ... β2-/- DCs were purified from potential other cells using the EasySepTM mouse Pan-DC enrichment kit (Stemcell Technologies) and allowed to rest for 1 h before the infection assay.
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bioRxiv - Neuroscience 2020Quote: Feeder-free E14Tg2a (Baygenomics) mouse embryonic stem cells (mES) were passaged on gelatin-coated (0.1% gelatin, StemCell Technologies) cell culture treated plastic dishes using established media and cell culture techniques (Sadegh and Macklis ...
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bioRxiv - Cancer Biology 2020Quote: ... Then DC were negatively selected using the EasySep Mouse Pan-DC Enrichment Kit (Stem Cell Technologies, Cat# 19763).
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bioRxiv - Cancer Biology 2022Quote: ... C57BL/6 CD8+ T cells were purified using the EasySep Mouse CD8 T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were enriched with the EasySep™ Mouse CD8 T Cell Isolation Kit (Stem Cell Technologies). Cells were stained ...
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bioRxiv - Cancer Biology 2020Quote: ... The EasySep™ Mouse Neutrophil Enrichment Kit was used as per the included protocol (StemCell Technologies, cat. 19762). Isolation cocktail containing antibodies and RapidSpheres™ were added to the whole murine blood sample and immunomagnetic negative selection was performed ...
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bioRxiv - Immunology 2021Quote: ... then neutrophils were negatively selected by using the EasySep™ mouse neutrophil enrichment kit (STEM cell technologies, #19762A), and 200,000 neutrophils/well were plated in 24-well plate on fibronectin coated glass coverslip ...
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bioRxiv - Immunology 2022Quote: Splenocyte-derived CD4+ T cells were isolated using Easysep Mouse CD4+ T cell isolation kit (19852, STEMCELL Technologies), and then 2e5 cells/well were plated in a 96 well plate ...
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bioRxiv - Cancer Biology 2022Quote: ... and the hematopoietic progenitors were isolated by magnetic negative selection (Mouse Hematopoietic Progenitor Cell Isolation Kit, StemCell Technologies), and cultivated in SFEM II media supplemented with 50ng/ml of murine IL6 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cell isolation was performed using EasySep Mouse CD8+ T cell Isolation Kit (#19853, STEMCELL Technologies, USA) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... cells were cultured into 96-well low-attachment plates containing NeuroCult Basal Media (Mouse and Rat; STEMCELL Technologies) with Proliferation Supplement (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... Macrophages were isolated from the peritoneal lavage using the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to PE ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD4+ T cells were isolated with EasySep Mouse Naïve CD4+ T Cell Isolation Kit (STEMCELL Technologies Inc) according to manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... then maintained in culture using reagents and protocol from the IntestiCult OGM Mouse Kit (Stemcell Technologies, Vancouver, Canada). Intestinal organoids were disassociated and resuspended into Corning® Matrigel® Growth Factor Reduced (GFR ...