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Citations for Stemcell Technologies :
551 - 569 of 569 citations for IL 4 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: CD4+ OT-II cells were isolated from spleen and LNs from OT-II*B6SJL mice with the EasySep mouse CD4+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada), according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... CD45 positive cells were depleted from tumor cell digests using the EasySep ™ Mouse streptavidin Rapidspheres™ Isolation Kit (Stem Cell Technologies, Catalog #19860) and a biotin conjugated anti Mouse CD45 antibody (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...
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bioRxiv - Cell Biology 2020Quote: Depletion of lineage-committed cells from single-cell suspensions of mouse bone marrow was performed using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit according to the manufacturer’s instructions (StemCell Technologies, Vancouver, BC, Canada).
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bioRxiv - Immunology 2021Quote: ... Cells from all mice were pooled by tissue type and B cells were selected from lymph node and spleen using a mouse Pan-B negative selection kit (Stemcell Technologies, Vancouver, BC, Canada). Generation of natively paired heavy and light chain libraries ...
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bioRxiv - Immunology 2020Quote: ... B cells were enriched by negative selection using EasySep mouse B cell enrichment kit as per manufacturer’s instructions (StemCell Technologies Inc., Tukwila, WA, USA). Enriched B cells were then resuspended in FACS buffer and stained and sorted as described previously(Carbonetti et al. ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Intermediate layer containing IELs was collected and washed with 1X PBS followed by either flowcytometry analysis or used for CD8α IELs purification using EasySep™ mouse CD8α positive selection kit-II (StemCell Technologies; Cat# 18953). Sort purified CD8α IELs (50,000/mouse ...
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bioRxiv - Immunology 2023Quote: ... Purification of CD8+ T cells for sorting or cell culture was performed using a mouse CD8+ T cell isolation kit (Stem Cell Technologies, Cat# 19853), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... malignant B cells were enriched from splenocytes of Eµ-TCL1 mice using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, Inc., Cologne, Germany). The CD5+ CD19+ content of purified cells was typically above 95% ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: Single cell suspensions of splenocytes were prepared and CD4+ T cells were isolated using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies; Cat # 19852A). CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
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bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell pellet was resuspended in 500 µL HBBS with 0.5% fatty acid-free BSA and lymphocytes were isolated by using the EasySep Mouse Streptavidin RapidSpheres isolation kit (STEMCELL Technologies, Vancouver, British Columbia, Canada) with the protocol provided ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... splenic T-lymphocytes were negatively selected from total splenocytes through use of EasySep Mouse T-cell Isolation Kit (Stem Cell Technologies #19851, Vancouver, BC, Canada). Samples were validated for >90% viability and purity before subsequent use.
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bioRxiv - Immunology 2020Quote: ... 1 x 105 T cells purified from spleen and lymph nodes with the EasySep Mouse T cell Isolation Kit (Stem Cell Technologies; Vancouver, BC, Canada) were cultured with 4 x 105 T-depleted splenocytes in 96-well round bottom plates in R10 media with 1 μg/mL αCD3 ...
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cells from each experimental group were transferred to a single 35 mm culture dish containing 1 mL of MethoCult GF M3534 culture medium (Methylcellulose medium with recombinant cytokines for mouse cells, Stem Cell Technologies, Vancouver, CA, catalog no. 03534) using a 3 mL syringe and 16-gauge needle ...