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Citations for Stemcell Technologies :
2951 - 3000 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Diluted blood was overlaid carefully on SepMate-15 tubes each containing up to 4 mL Lymphoprep (STEMCELL Technologies, catalog #07851). The layered blood was centrifuged at 1,200 × g for 10 minutes at RT ...
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bioRxiv - Genetics 2023Quote: Differentiation of iPSCs into ECs was performed using STEMdiff Hematopoietic Kit (StemCell Technology, 05310). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Cells were maintained in Stem Span (StemCell Technologies) with 100 ng/ml SCF ...
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bioRxiv - Cell Biology 2023Quote: ... were passaged into replicate plates using Gentle Cell Dissociation Reagent (STEMCELL Technologies). For screening ...
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bioRxiv - Genetics 2023Quote: ... #L2020)-coated plates and differentiated into neuronal precursors using STEMdiff Forebrain Neuron Differentiation Kit (STEMCELL Technologies; #08600), and the cells were further matured using STEMdiff Forebrain Neuron Maturation Kit (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... 500µL BrainPhys Neuronal Medium (Stemcell Technologies) and 10µL additional DNase I were added ...
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bioRxiv - Immunology 2023Quote: ... UCB mononuclear cells (UCBMC) and plasma were isolated after whole-blood centrifugation over LymphoPrep in SepMate tubes (STEMCELL Technologies, Vancouver, BC) following manufacturers’ protocols ...
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bioRxiv - Immunology 2023Quote: Neutrophils of healthy blood donors were isolated using EasySep™ Direct Human Neutrophil Isolation Kit (Stemcell Technologies). Neutrophils were stimulated with immune-complexes as described above and supernatants collected after 4 h ...
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bioRxiv - Microbiology 2023Quote: ... 10 µM DAPT (STEMCELL Technologies) was added to cells cultured in 150mm tissue culture flasks containing 30 mL DMEM + 10% FBS + Glutamax and incubated for 3-4 days in a 37°C incubator with 5% CO2 atmosphere until confluent to induce differentiation of goblet cells ...
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bioRxiv - Immunology 2023Quote: ... Dissociation media contained 0.5 U/mL dispase (Stemcell Technologies, 07923), 0.1 mg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR; Stem Cell Technologies, 05872). Cells were checked for mycoplasma contamination every two weeks ...
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bioRxiv - Neuroscience 2023Quote: ... and SB-431542 (StemCell Technologies, Cat. No. 72234) are added to the media ...
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bioRxiv - Neuroscience 2023Quote: ... EBs were cultured in AggreWell™800 (StemCell Technologies, Cat. No. 34815) plates rinsed with Anti-Adherence solution (StemCell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Neuroepithelial spheroids are maintained is suspension in neural media (DMEM-F12, N2/B27 supplements, Heparin (Stemcell Tech, Cat. No. 07980), Anti-Anti ...
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bioRxiv - Neuroscience 2023Quote: ... Neural rosette formation occurs at day 14 where rosettes were selected for with STEMdiff™ Neural Rosette Selection Reagent (StemCell Technologies, Cat. No. 05832). NPCs were expanded in NPC media which contained DMEM/F12 + 1x N2 + 1x B27-VA supplemented with FGF2 (Biotechne ...
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bioRxiv - Neuroscience 2023Quote: ... iAssembloids were equilibrated in BrainPhys™ Neuronal Medium (StemCell Technologies, Cat. No. 05790) 24 hours before plating to facilitate comparison to 2D monocultured neurons ...
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bioRxiv - Neuroscience 2023Quote: ... plates rinsed with Anti-Adherence solution (StemCell Technologies, Cat. No. 07010) for 7 days and then plated onto a Matrigel coated plate ...
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bioRxiv - Molecular Biology 2023Quote: Naïve CD4+ T cells from spleen and lymph nodes were enriched by negative selection using EasySepTM Mouse Naïve CD4+ T cell isolation kit (STEMCELL Technologies). 100,000 cells/well (50,000 CD45.1- cells and 50,000 CD45.1+ cells ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were enriched from spleen and lymph nodes of CD45.1+ mice by negative selection using EasySepTM Mouse CD4+ T cell isolation kit (STEMCELL Technologies), and FACS sorted as CD4+CD25- CD44loCD62Lhi cells ...
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bioRxiv - Cell Biology 2023Quote: ... cells were passaged and seeded as above and cultured in complete STEMdiffTM Astrocyte Maturation Medium (AMM) (Stemcell Technologies, #08550) with medium changed every other day ...
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bioRxiv - Cell Biology 2023Quote: ... cells were dissociated into single cells with Accutase (Stemcell Technologies, #07920) and transfered into a new Laminin pre-coated plate with medium changed every other day ...
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bioRxiv - Cell Biology 2023Quote: ... medium was changed to 2 mL of complete STEMdiffTM Astrocyte Differentiation Medium (ADM) (Stemcell Technologies, #08540). On day7 and day 14 ...
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bioRxiv - Cell Biology 2023Quote: ... Rosettes were isolated from cultures using Neural Rosette Selection Reagent (Stemcell Technologies, #05832), this was the NPC stage.
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bioRxiv - Developmental Biology 2023Quote: Human pluripotent stem cells were cultured in the pluripotency-maintenance media mTeSR1 (StemCell Technologies) on Cultrex (R&D Systems)-coated 35mm tissue culture plates ...
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bioRxiv - Developmental Biology 2023Quote: ... pre-coated as follows: 500μl anti-adherent solution (STEMCELL Technologies) was added to each well for 5 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: ... hESC was maintained and expanded in feeder-free culture conditions with mTeSR basal medium (STEMCELL Technologies Inc., Vancouver, Canada, cat # 85850) on 6-well plates culture-treated coated with Corning Matrigel hESC-qualified Matrix ...
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bioRxiv - Cell Biology 2023Quote: Accutase-digested (STEMCELL Technologies 07920) enteroids were resuspended in ENR with 1:4 lentivirus solution ...
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bioRxiv - Developmental Biology 2023Quote: ... derived from the parental WTC-11 hiPSC were obtained from the Coriell Institute and generated at the Allen Institute for Cell Science at passage 29 frozen in mTeSR™ Plus medium (STEMCELL Technologies). The PAX2-EGFP-iPSC line was generated starting from BJ2 iPSC lines at the Harvard Stem Cell Institute core facility ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 μM β-mercaptoethanol (Gibco], supplemented with 10 ng/mL mouse LIF (STEMCELL Technologies), 3 μM CHIR99021 (STEMCELL Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... 3 μM CHIR99021 (STEMCELL Technologies), 1 μM PD0325901 (STEMCELL Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... Aggrewell™400 was prepared by rinsing the wells with an anti-adherence rinsing solution (Stem cell technologies) as per the manufacturer’s protocol and washed with SL (without LIF ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μM PD0325901 (STEMCELL Technologies)) and passaged as single cells onto fresh feeders using TrypLE Express (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... pre-coated 6-well plates in mTeSR1 complete medium (85850, STEMCELL Technologies).
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bioRxiv - Cell Biology 2023Quote: Enteroids were dissociated to single cells using Accutase (STEMCELL Technologies 07920), resuspended in ENR supplemented with 10 µM Y-27632 ...
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bioRxiv - Bioengineering 2023Quote: IMR90-4 iPSCs (WiCell, Madison, WI) were cultured on Matrigel-coated 6-well plates and supplemented daily with E8 medium (Stem Cell Technologies) as described (81) ...
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bioRxiv - Genomics 2023Quote: Human CD8+ T cells were obtained from either pooled PBMC donors (ZenBio) using negative selection human CD8 isolation kits (StemCell Technologies) or directly from vials containing isolated CD8+ T cells from individual donors (StemCell Technologies) ...
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bioRxiv - Genomics 2023Quote: ... mTeSR1 media (Stemcell Technologies) was replaced with mesoderm induction media (Stemcell Technologies 05221).
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bioRxiv - Genomics 2023Quote: ... single hESC cells were plated at a density of approximately 5×105 per each well of a 6 well plate in the presence of ROCK inhibitor and exposed to commercially available mesoderm induction media (Stemcell Technologies 05221).
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bioRxiv - Genomics 2023Quote: ... was replaced with mesoderm induction media (Stemcell Technologies 05221).
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bioRxiv - Genomics 2023Quote: ... in the presence of ROCK inhibitor and exposed to commercially available mesoderm induction media as instructed by the supplier (Stemcell Technologies 05221). After four days of treatment cells were dissociated into single cells with Accutase (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: ... or directly from vials containing isolated CD8+ T cells from individual donors (StemCell Technologies). For all technology development experiments ...
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bioRxiv - Genomics 2023Quote: ... selected for Thy1.1 using a CD90.1 Positive Selection Kit (StemCell Technologies), and then stained for Thy1.1 and IL2RA ...
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bioRxiv - Genomics 2023Quote: ... in mTeSR1 media (Stemcell Technologies). Cells were routinely passaged as clumps after dissociation with 10uM EDTA for 3 minutes as needed ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using an EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2023Quote: ... and 2 H3.1-K27M (GSC3, GSC4) cellular models were grown in NeurocultTM medium supplemented with heparin (2 μg/mL, Stemcell Technologies), EGF/FGF (20ng/mL ...
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bioRxiv - Cell Biology 2023Quote: ... and 200 µg/ml DNase-I (Stem Cell Technologies, Catalog#07900) in Intestinal Epithelial Stem Cell Media (ISC Media ...
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bioRxiv - Bioengineering 2023Quote: ... medium in apical and basal chamber was switched to PneumaCult™-ALI medium (STEMCELL Tech.) containing 1% penicillin/streptomycin ...
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bioRxiv - Bioengineering 2023Quote: ... The following primary antibodies were used: beta-tubulin III (STEMCELL Technologies 60052; 1:1000), GFAP (Dako GA524 ...
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bioRxiv - Cell Biology 2023Quote: ... and 2.5µM of IWP-2(Stemcell Technologies). Rudibaugh2 organoid media was supplemented with 100ng/mL SHH(VWR ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated cells were then reaggregated in low adhesion microwell culture plates (AggreWell-800, Stem cell technologies). 3 million cells were plated per 2mL well in mTesR plus Y-27632 (1 mM ...