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2851 - 2900 of 8825 citations since 2019

• Maximal interferon induction by influenza lacking NS1 is infrequent owing to requirements for replication and export

  Alison C. Vicary, et al., bioRxiv - Microbiology 2023

  Quote: ... NHBE cells were maintained in filter-sterilized PneumaCult-Ex-PLUS Medium (Stem Cell Technologies #05040) supplemented with 1% penicillin-streptomycin ...
• Interferon-γ Elicits Pathological Hallmarks of ALS in Human Motor Neurons

  Changho Chun, et al., bioRxiv - Neuroscience 2023

  Quote: WTC11 (WT, Q331K+/-) iPSCs were frozen in mFreSR medium (Stem Cell Technologies) and stored under cryogenic conditions in liquid nitrogen ...
• Local production of reactive oxygen species drives vincristine-induced axon degeneration

  Jorge Gómez-Deza, et al., bioRxiv - Neuroscience 2023

  Quote: ... Cells were plated in neuronal maturation media (BrainPhys medium (STEMCELL Technologies, cat. no. 05790), 1× B27 Plus Supplement (ThermoFisher Scientific ...
• The NQR complex regulates the immunomodulatory function of Bacteroides thetaiotaomicron

  Morgan J. Engelhart, et al., bioRxiv - Microbiology 2023

  Quote: In vitro IL-10 assays were also performed using human Peripheral Blood Mononuclear Cells (PBMCs) (STEMCELL Technologies, Cat. # 70025.1). The assay was performed as described above ...
• Homozygous ALS-linked mutations in TARDBP/TDP-43 lead to progressive synaptic dysfunction in human iPSC-derived motor neurons

  Sarah Lépine, et al., bioRxiv - Neuroscience 2023

  Quote: ... and passaged at 80% confluence using Gentle Cell Dissociation Reagent (StemCell Technologies). Cultures were routinely tested for mycoplasma using the MycoAlert Mycoplasma Detection kit (Lonza).
• Oligodendrocytes in human iPS cell-derived cortical grafts remyelinate adult rat and human cortical neurons

  Raquel Martinez-Curiel, et al., bioRxiv - Neuroscience 2023

  Quote: ... media was changed to Brain Phys (StemCell Technologies) supplemented with B27 without retinoid acid (1:50 ...
• Single cell transcriptomic landscapes of human liver organoids stratify models of non-alcoholic fatty liver disease

  Anja Hess, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Rho-associated kinase (ROCK)-Inhibitor (StemCell Technologies, Y-27632) was added to mTeSR at 10 µM ...
• Single cell transcriptomic landscapes of human liver organoids stratify models of non-alcoholic fatty liver disease

  Anja Hess, et al., bioRxiv - Molecular Biology 2023

  Quote: ... hPSCs were maintained in mTeSR medium (StemCell Technologies) on Matrigel (Corning ...
• Interferon-γ Elicits Pathological Hallmarks of ALS in Human Motor Neurons

  Changho Chun, et al., bioRxiv - Neuroscience 2023

  Quote: ... The supernatant was aspirated and cells were resuspended in fresh mTeSR (Stem Cell Technologies) supplemented with 10 µM Y-27632 (Thermo Fisher Scientific) ...
• Interferon-γ Elicits Pathological Hallmarks of ALS in Human Motor Neurons

  Changho Chun, et al., bioRxiv - Neuroscience 2023

  Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
• Cytokine signaling converging on IL11 in ILD fibroblasts provokes aberrant epithelial differentiation signatures

  Miriam T. Kastlmeier, et al., bioRxiv - Cell Biology 2023

  Quote: ... or Gentle Cell Dissociation Reagent (StemCell Technologies) (Jacob et al. ...
• Cytokine signaling converging on IL11 in ILD fibroblasts provokes aberrant epithelial differentiation signatures

  Miriam T. Kastlmeier, et al., bioRxiv - Cell Biology 2023

  Quote: ... Cells were subcultured by using ReLeSR (StemCell Technologies) or Gentle Cell Dissociation Reagent (StemCell Technologies ...
• Differential Regulation of Retinoic Acid Metabolism in Fanconi Anemia

  Justin L. Blaize, et al., bioRxiv - Biochemistry 2023

  Quote: Cells were counted and adjusted to a concentration of 5 x 105 cells/mL with ALDEFLUOR assay (01700; Stem Cell Technologies) buffer ...
• Cytokine signaling converging on IL11 in ILD fibroblasts provokes aberrant epithelial differentiation signatures

  Miriam T. Kastlmeier, et al., bioRxiv - Cell Biology 2023

  Quote: ... (StemCell Technologies). On day 14 of differentiation ...
• Leukemic stem cells hijack lineage inappropriate signalling pathways to promote their growth

  Sophie G Kellaway, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 750nM SR1 (Stemcell Technologies), 150ng/ml SCF ...
• MAP kinase ERK5 modulates cancer cell sensitivity to extrinsic apoptosis induced by death-receptor agonists and Natural Killer cells

  Sergio Espinosa-Gil, et al., bioRxiv - Cancer Biology 2023

  Quote: ... recombinant human Noggin (100 ng/ml, Stemcell Technologies), B-27 supplement (ThermoFisher) ...
• Endogenous viral elements constitute a complementary source of antigens for personalized cancer immunotherapy

  Christian Garde, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and viability dye (GloCellTM Fixable Viability Dye, Stem Cell Tech #75010) at 4 °C for 30 minutes ...
• Leukemic stem cells hijack lineage inappropriate signalling pathways to promote their growth

  Sophie G Kellaway, et al., bioRxiv - Cancer Biology 2023

  Quote: ... in SFEMII (StemCell Technologies) supplemented with 1% Pencillin/Streptomycin ...
• T-FINDER: A highly sensitive, pan-HLA platform for functional T cell receptor and ligand discovery

  Theresa Schmid, et al., bioRxiv - Immunology 2023

  Quote: ... B cells were extracted by negative selection with the EasySep™ Human B Cell Isolation Kit (StemCell Technologies, #17954). EBV-containing supernatants from B95-8 cells were added to purified B cells supported by 30 µg mL-1 holo-transferrin and 2.5 µg mL-1 CpG (ODN2006) ...
• Inosine Induces Stemness Features in CAR T cells and Enhances Potency

  Dorota D. Klysz, et al., bioRxiv - Immunology 2023

  Quote: ... Primary human T cells were isolated using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) according to the manufacturer’s protocol ...
• Inosine Induces Stemness Features in CAR T cells and Enhances Potency

  Dorota D. Klysz, et al., bioRxiv - Immunology 2023

  Quote: ... Isolated T cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stem Cell Technologies).
• A common human MLKL polymorphism confers resistance to negative regulation by phosphorylation

  Sarah E. Garnish, et al., bioRxiv - Immunology 2023

  Quote: ... Successfully transduced cells were selected using puromycin (2.5µg/mL; StemCell Technologies) using established procedures18 ...
• Loss of Rab8a in B cells leads to increased antibody responses and class-switch recombination

  Sara Hernández-Pérez, et al., bioRxiv - Immunology 2023

  Quote: Splenic B cells or T cells were Isolated with EasySepTM Mouse B Cell Isolation Kit (#19854, StemCell Technologies) or EasySepTM Mouse T Cell Isolation Kit (#19851 ...
• Loss of Rab8a in B cells leads to increased antibody responses and class-switch recombination

  Sara Hernández-Pérez, et al., bioRxiv - Immunology 2023

  Quote: ... or EasySepTM Mouse T Cell Isolation Kit (#19851, StemCell Technologies) according to the manufacturer’s instructions and let to recover in cRPMI (10% FCS ...
• Dandelion utilizes single cell adaptive immune receptor repertoire to explore lymphocyte developmental origins

  Chenqu Suo, et al., bioRxiv - Immunology 2023

  Quote: A vial of frozen PBMCs was acquired from Stemcell Technologies with informed consent (as stated by Stemcell Technologies) and approval from the Yorkshire & The Humber - Leeds East Research Ethics Committee (19/YH/0441) ...
• Dandelion utilizes single cell adaptive immune receptor repertoire to explore lymphocyte developmental origins

  Chenqu Suo, et al., bioRxiv - Immunology 2023

  Quote: A vial of frozen PBMCs was acquired from Stemcell Technologies with informed consent (as stated by Stemcell Technologies ...
• YAP controls cell migration and invasion through a Rho-GTPase switch

  Sagar R. Shah, et al., bioRxiv - Cancer Biology 2023

  Quote: ... or 5 μg/cm2 collagen for MCF10A cells (Stem Cell Technologies). 48 hours after seeding ...
• The Alzheimer’s disease risk gene BIN1 modulates neural network activity via the regulation of L-type calcium channel expression in human-induced neurons

  Orthis Saha, et al., bioRxiv - Neuroscience 2023

  Quote: ... hiPSCs are cultured and passaged for 3 times in STEMdiff™ Neural Induction Medium + SMADi (Stemcell Technologies) for up to 3 weeks ...
• The Alzheimer’s disease risk gene BIN1 modulates neural network activity via the regulation of L-type calcium channel expression in human-induced neurons

  Orthis Saha, et al., bioRxiv - Neuroscience 2023

  Quote: ... were maintained in media from Stemcell Technologies, Vancouver ...
• The Alzheimer’s disease risk gene BIN1 modulates neural network activity via the regulation of L-type calcium channel expression in human-induced neurons

  Orthis Saha, et al., bioRxiv - Neuroscience 2023

  Quote: ... and transferred to a 24-well ultra-low adherent plate with Expansion Medium (Stem Cell Technologies). After three days ...
• The Alzheimer’s disease risk gene BIN1 modulates neural network activity via the regulation of L-type calcium channel expression in human-induced neurons

  Orthis Saha, et al., bioRxiv - Neuroscience 2023

  Quote: ... complete BrainPhys medium (StemCell Technologies) is added 1:1 together with 2 µg/mL doxycycline (Sigma-Aldrich ...
• The Alzheimer’s disease risk gene BIN1 modulates neural network activity via the regulation of L-type calcium channel expression in human-induced neurons

  Orthis Saha, et al., bioRxiv - Neuroscience 2023

  Quote: ... the medium was replaced by Maturation Medium (Stem Cell Technologies) and the plate was placed in an orbital shaker (100 rpm speed) ...
• The Alzheimer’s disease risk gene BIN1 modulates neural network activity via the regulation of L-type calcium channel expression in human-induced neurons

  Orthis Saha, et al., bioRxiv - Neuroscience 2023

  Quote: ... using STEMdiff™ Neural Progenitor Medium (NPM; Stemcell Technologies). PLO solution was made in water (0.001% ...
• In vivo functional genomics identifies essentiality of potassium homeostasis in medulloblastoma

  Jerry J. Fan, et al., bioRxiv - Cancer Biology 2023

  Quote: ... using Neurocult NS-A Basal media (StemCell Technologies) supplemented with 2 mM L-glutamine ...
• In vivo functional genomics identifies essentiality of potassium homeostasis in medulloblastoma

  Jerry J. Fan, et al., bioRxiv - Cancer Biology 2023

  Quote: ... followed by treatment using 50% Accutase (Stemcell Technologies) diluted in PBS ...
• Conditional blastocyst complementation of a defective Foxa2 lineage efficiently promotes generation of the whole lung

  Akihiro Miura, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and 15 U/ml Dispase II (Stemcell Technologies, 7913) in HBSS) ...
• Regulation of Store-Operated Ca²⁺ Entry by IP₃ Receptors Independent of Their Ability to Release Ca²⁺

  Pragnya Chakraborty, et al., bioRxiv - Cell Biology 2023

  Quote: ... CHIR99021 (3 μM, STEMCELL Technologies, Cat#72052), purmorphamine (0.5 mM ...
• Regulation of Store-Operated Ca²⁺ Entry by IP₃ Receptors Independent of Their Ability to Release Ca²⁺

  Pragnya Chakraborty, et al., bioRxiv - Cell Biology 2023

  Quote: ... purmorphamine (0.5 mM, STEMCELL Technologies, Cat#72202) and ascorbic acid (150 μM ...
• The NQR complex regulates the immunomodulatory function of Bacteroides thetaiotaomicron

  Morgan J. Engelhart, et al., bioRxiv - Microbiology 2023

  Quote: ... 100 μg/mL of DNase 1 (STEMCELL Technologies, Cat. # 07900) was added to the cell suspension for 15 minutes at room temperature to prevent cell clumping ...
• One for all – Human kidney Caki-1 cells are highly susceptible to infection with corona- and other respiratory viruses

  A. Daniels, et al., bioRxiv - Microbiology 2023

  Quote: ... Cells were detached from a T75 using Gentle Cell Dissociation Reagent (Stemcell Technologies) and recovered in DMEM prior to staining ...
• Genomic characteristics and clinical significance of CD56+ Circulating Tumor Cells in Small Cell Lung Cancer

  C. Ricordel, et al., bioRxiv - Cancer Biology 2023

  Quote: The first step consists in a CTC enrichment from whole blood sample by immunomagnetic negative selection using the EasySep™ Direct Human CTC Enrichment Kit (StemCell Technologies®, Vancouver, Canada) according to manufacturer instruction ...
• Ultrastructural comparison of human kidney organoids and human fetal kidneys reveals features of hyperglycemic culture

  Anika Schumacher, et al., bioRxiv - Bioengineering 2023

  Quote: ... were differentiated towards kidney tissue and the formed organoids were cultured as described in our previous work.(14) Briefly, the iPSC line LUMC0072iCTRL01 (Leiden University Medical Center, the Netherlands) was differentiated in APEL2 medium (STEMCELL Technologies) supplemented with 1% protein-free hybridoma medium (PFHMII ...
• Neoepitope-specific vaccination of a patient with diffuse midline glioma targeting H3K27M induces polyclonal B and T cell responses across diverse HLA alleles

  Tamara Boschert, et al., bioRxiv - Cancer Biology 2023

  Quote: B cell isolation was performed using the the EasySep Human B Cell Isolation Kit (StemCell Technologies). PBMC were diluted in PBS supplemented with 2 % FBS and 1 mM EDTA to a final concentration of 107 cells/ml ...
• Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model

  Ole Vidhammer Bjørnstad, et al., bioRxiv - Cancer Biology 2023

  Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
• Complex genotype-phenotype relationships shape the response to treatment of Down Syndrome Childhood Acute Lymphoblastic Leukaemia

  Christoph Lutz, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD34+ cells were enriched by magnetic bead separation (StemCell Technologies or Miltenyi). After thawing ...
• Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model

  Ole Vidhammer Bjørnstad, et al., bioRxiv - Cancer Biology 2023

  Quote: ... “Monolayer Culture Protocol” from “Generation and Culture of Neural Progenitor Cells Using the STEMdiff Neural System” (STEMCELL Technologies) was used to generate third passage NPC-like cells ...
• Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model

  Ole Vidhammer Bjørnstad, et al., bioRxiv - Cancer Biology 2023

  Quote: Spheroids were dissociated using Accutase (STEMCELL Technologies), incubated on shaker table for 10 minutes at 37°C before being manually pipetted until a single cell suspension remained ...
• Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model

  Ole Vidhammer Bjørnstad, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Accutase (STEMCELL Technologies) was further used to disassociate NPC cells ...
• Adaptation to ex vivo culture drives human haematopoietic stem cell loss of repopulation capacity in a cell cycle independent manner

  Carys S. Johnson, et al., bioRxiv - Cell Biology 2023

  Quote: ... human LDL (50ng/ml) (Stem Cell Technologies) in addition to the following cytokines ...
• A Resource of Gene Expression Data from a Multiethnic Population Cohort of Induced Pluripotent Cell–Derived Cardiomyocytes

  Wenjian Lv, et al., bioRxiv - Genomics 2023

  Quote: ... Undifferentiated iPSCs were detached by a 4-min incubation with Accutase (StemCell technologies) and seeded onto Geltrex (Life Technologies)-coated plates at a density of 200,000 cells/cm2 ...