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Citations for Stemcell Technologies :
251 - 300 of 2099 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... edited tCD19+ cells were enriched using the EasySep™ Human CD19 Positive Selection Kit II (STEMCELL Technologies). All cells were rested overnight by culturing in the presence of 100 IU/mL (Tregs ...
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bioRxiv - Immunology 2024Quote: ... which involved magnetic bead separation employing EasySep Direct Human Monocyte Isolation Kit (StemCell Technologies, Vancouver, BC, CAN). This technique ensured the attainment of a highly pure monocyte population with minimal contamination from other cell types ...
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bioRxiv - Immunology 2024Quote: ... Total CD8+ T cells were isolated using EasySep Human CD8+ T Cell Isolation Kit (StemCell Technologies, #17953). Screening for HLA-A*02-positive individuals was performed with an anti-human HLA-A2 Antibody (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Polyclonal CD8+ T cells were selected using EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies; 17953). Blood monocytes were isolated through negative magnetic sorting (EasySep Human Monocyte Enrichment Kit ...
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Biochemistry 2024Quote: Human monocytes (StemCell Technologies) were maintained in DMEM (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... BMPCs were enriched from bone marrow mononuclear cells using EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% dimethyl sulfoxide in FBS.
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bioRxiv - Immunology 2021Quote: ... B cells were enriched from PBMC by first using EasySep Human Pan-B cell Enrichment Kit (StemCell Technologies), and then stained with CD20-PB (2H7 ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from whole blood with the EasySepTM Direct Human NK Cell Isolation Kit (StemCell Technologies). The gating strategy for isolation of CD56+CD3- NK cells is shown in Figure S1 (Figure 2 – Supplement 1) ...
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bioRxiv - Immunology 2022Quote: ... CD14+ CD16- monocytes were isolated using magnetic negative selection (EasySep Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, Massachusetts) and differentiated into macrophages by culture in RPMI 1640 (Lonza ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated from PBMC by negative selection using the EasySep™ Human Monocyte Isolation Kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... 12 w samples were further subjected to EasySep™ Human EpCAM Positive Selection Kit II (STEMCELL Technologies, 18356) to positively select for epithelial cells ...
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bioRxiv - Immunology 2022Quote: ... and CD4 T cells were isolated by immunomagnetic positive selection (EasySep Human CD4 positive selection kit; Stemcell Technologies). Sex and age for these individuals can be found in the metadata corresponding to the RNA-Seq data available from the Sequence Read Archive under accession number PRJNA832136.
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bioRxiv - Immunology 2020Quote: ... prior to CD4 and CD8 T cells isolation using EasySep Human T cell isolation kit (STEMCELL Technology, #17951). Isolated T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... CD34+ cell enrichment was performed using EasySep™ Human CD34 Positive Selection Kit II (StemCell Technologies, Cat 17856) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... positive immunomagnetic selection of CD45+ cells were performed with EasySep Human CD45 Depletion Kit II (StemCell Technologies, Canada) according to the manufacturer instructions (post-isolation purity 96-99%) ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... Bulk T cells were negatively selected using the EasySepTM Human T Cell Enrichment Kit (STEMCELL Technologies, Cambridge, MA), and 7.5 x 105 T cells were added per well to the MDC1 containing wells (MDC1 to T cell ratio = 1:10) ...
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bioRxiv - Immunology 2020Quote: ... Naive B cells (CD19+CD27−IgD+) were enriched using Human naive B cell negative selection kit (Stemcell Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... The EasySep™ Direct Human Neutrophil Isolation Kit was used as per standard protocol (StemCell Technologies, cat. 19666). Isolation cocktail containing antibodies and RapidSpheres™ were added to the whole blood sample and immunomagnetic negative selection was performed ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and CD8+ T cells isolated using the EasySep Human CD8+ T Cell Isolation kit (Stemcell Technologies, #17953). Primary human CD8+ T cells were cultured for up to 24 days in ATCC-modified RPMI (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were obtained by negative selection using the EasySep Human CD4+ Enrichment kit (Stem Cell Technologies), and stimulated with anti-CD3-coated magnetic beads (Dynabeads M-450 tosyl-activated ...
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bioRxiv - Immunology 2022Quote: ... eNK cells added to T cell cultures were removed (EasySep Human CD56 Positive Selection Kit II, STEMCELL Technologies) prior to analysis ...
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bioRxiv - Immunology 2024Quote: ... Naïve T cells were isolated by EasySep™ Human Naïve Pan T Cell Isolation Kits (STEMCELL Technologies, 17961). Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... and CD14+ cells were purified using the EasySep™ Human CD14 Positive Selection Kit II (StemCell Technologies, 17858). A total of 1.5×106/well CD14+ monocytes were seeded in a 12-well plate with 50 ng/mL of hM-CSF (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... CD34+ cells were isolated using EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, Vancouver, BC, Canada) as per manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... naive Treg cells were purified by using EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies). For human cell culture ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated from PBMCs with the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies). Genomic DNA was isolated from a median of 3.65 million CD4+ T cells (IQR 2.48-4.98 ...
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bioRxiv - Immunology 2023Quote: ... B cells were purified from TMNCs with the EasySep Human CD19 Positive Selection Kit II (Stem Cell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were isolated from PBMCs using EasySep™ Human T Cell Isolation Kit (StemCell Technologies, Taiwan) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The human iPSC-derived cardiomyocytes (hiPSC-CMs) were reprogrammed using the STEMdiff™ Cardiomyocyte Differentiation Kit (STEMCELL Technologies) as recommended by the manufacturer ...
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bioRxiv - Immunology 2023Quote: PBMCs were obtained for CD14 positive monocyte isolation using the EasySep Human Monocyte Isolation Kit (Stem cell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... CD34+ cells were isolated from the purified PBMNCs by EasySep Human CD34 positive selection kit II (STEMCELL Technologies) and expanded in HSC expansion media as described earlier (Genovese et al ...
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bioRxiv - Bioengineering 2023Quote: Monocytes were isolated from healthy donors’ blood using EasySep™ Human Monocyte Isolation Kit (Stemcell technologies, Cambridge, MA) by the MIT monocyte core facility ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were isolated by immuno-magnetic negative selection using the EasySep Direct human neutrophil isolation kit (StemCell Technologies). A highly pure population of neutrophils was then generated by cell sorting on the basis of size ...
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bioRxiv - Cell Biology 2024Quote: The CD34+ HSC cells were isolated using Human Cord Blood CD34 Positive Selection Kit II (Stemcell Technologies, #17896) as per the supplier instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by CD34 magnetic bead-based enrichment using the EasySep human CD34 positive selection kit II (StemCell technologies). We found this enrichment strategy to be simple and reproducible and chose it for several reasons ...
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bioRxiv - Bioengineering 2024Quote: ... Human T cells were isolated from thawed cryostocks using an EasySep CD3+ T cell isolation kit (StemCell Technologies). T cells were cultured at a 1:2 cell:bead ratio with cultured αCD3/αCD28 activator beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: CD14+ cells were isolated from PBMCs using the EasySep™ Human CD14 Positive Selection Kit II (STEMCELL Technologies) to a purity of > 97% ...
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bioRxiv - Cell Biology 2024Quote: ... CD11b-ATM was prepared by EasySep Human CD11b Positive Selection and Depletion Kit (STEMCELL technologies #ST-100-0744).
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bioRxiv - Cancer Biology 2024Quote: ... γδT cells were magnetically sorted using EasySep™ Human Gamma/Delta T Cell Isolation Kit (Stemcell Technologies, 19255) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Human PBMCs were activated with ImmunocultTM CD3/CD28 Human T cell Activators (STEMCELL Technologies, anti-human CD3 and anti-human CD28 monospecific antibody complexes ...
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bioRxiv - Systems Biology 2020Quote: ... CD66b and glycophorin A were depleted from PBMCs using magnetic sorting (Human Pan-DC Pre-Enrichment Kit, StemCell Technologies). pDCs were then sorted on a FACS Vantage instrument (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells were isolated from PBMCs via the Easysep Human CD4+ T-cell negative enrichment kit (STEMCELL Technologies). The isolated human CD4+ T cells were cultured at 2.5 × 106 cells/ml in complete RPMI medium (cRPMI) ...
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bioRxiv - Immunology 2021Quote: T cells were isolated from PBMCs with EasySep™ Human T cell Isolation Kit per manufacturer’s instructions (StemCell Technologies). Isolated T cells (0.5-1 × 105 per well ...
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bioRxiv - Immunology 2022Quote: Isolation of neutrophils was carried out EasySep™ Direct Human Neutrophil Isolation Kit (Stem Cell Technologies, Vancouver, Canada, 19666), an immunomagnetic negative selection kit ...
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bioRxiv - Immunology 2020Quote: ... T cells were purified from thawed PBMCs using the EasySep Human T cell enrichment kit (STEMCELL Technologies, Cambridge, MA). RNA was extracted from purified human T cells using the RNeasy Plus Minikit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... plasma cells were isolated using the EasySep Human CD138 Positive Selection Kit II for future studies (Stemcell Technologies, #17877). The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit ...
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bioRxiv - Genetics 2021Quote: ... we performed immunohistochemistry in 11-week-old human cerebral organoids generated with the STEMdiff cerebral organoid kit (STEMCELL Technologies), and C57BL/6J mouse brains (Jackson laboratory ...
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bioRxiv - Immunology 2020Quote: CD4+T-cells were isolated from PBMCs with the EasySep™ human CD4+T-cell enrichment kit (StemCell Technologies). T-cells/PBMCs were added to cultured hepatic epithelial cell lines (Huh-7 ...