Labshake search
Citations for Stemcell Technologies :
501 - 550 of 2099 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
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bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
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bioRxiv - Immunology 2023Quote: ... CD34+ human hematopoietic stem/progenitor cells (hHCs) were isolated using the EasySep™ Human CD34 positive selection kit according to manufacturer’s instructions (StemCell Technology, Vancouver, Canada). These cells were cultured ex vivo using various protocols ...
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bioRxiv - Cell Biology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Primary NK cells were isolated from PBMCs by immunomagnetic negative selection using EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies Canada, Inc.) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... Mice were humanized as we described previously [36] with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies, Belgium). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ T cells were isolated from frozen PBMC tubes using the EasySep™ Human CD4+ T Cell Isolation Kit (Stem Cell Technologies, #17952) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Human neutrophils were purified from whole blood of healthy volunteers by immunomagnetic negative selection (EasySepTM Direct Human Neutrophil Isolation Kit, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy Sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy Sep direct human monocyte isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... human PBMCs (AllCells) were thawed and CD4+ T cells were isolated using the EasySep™ Human CD4+ T Cell Isolation Kit (Stem Cell Technologies). Naive CD4+ T cells were resuspended in complete RPMI (cRPMI ...
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bioRxiv - Molecular Biology 2024Quote: CD34+ cells were isolated from ficolled bone marrow mononuclear cells using the EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, 17856) according to the manufacturer’s instructions in PBS containing 2% fetal bovine serum and 1 mM EDTA ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Bioengineering 2021Quote: ... Primary human T cells were isolated using negative selection per the manufacturer’s instructions (RosetteSep Human T Cell Enrichment Cocktail, STEMCELL Technologies). T cells were counted using a Countess II FL Automated Cell Counter (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... total PBMCs or purified human naïve CD4+ T cells were stimulated with the ImmunoCult human T cell activator (StemCell Technologies) in the presence of either DMSO or soquelitinib for 72 hours ...
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bioRxiv - Cancer Biology 2022Quote: TS543 human glioma cell line was cultured in human NeuroCult™ NS-A Basal Medium (Stem Cell Technologies, cat #05751), supplemented with 10% NeuroCult™ Proliferation Supplement ...
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bioRxiv - Immunology 2024Quote: ... Human T cells were then isolated from a diluted buffy coat using RosetteSep Human T Cell Enrichment Cocktail (StemCell Technologies) and SepMate tubes (StemCell Technologies ...
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bioRxiv - Genomics 2024Quote: ... supplemented with required additional growth factors (20 ng/mL human recombinant EGF, 10 ng/mL human recombinant bFGF, 2μg/mL heparin, STEMCELL Technologies). Cultures were seeded on ultra-low attachment culture ware (Corning CLS3471 ...
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bioRxiv - Immunology 2022Quote: ... recombinant human FGF (25ug/mL; Stem Cell Technologies), and recombinant human EGF (10 ug/mL ...
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bioRxiv - Immunology 2020Quote: ... Human Recombinant IFNγ (10ng/mL, StemCell Technologies 78020) was added to the media for a period of 60 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and RosetteSep Human CD8 Depletion Cocktail (Stemcell Technologies) per the manufacturer’s instructions ...
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Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... recombinant EGF (20 ng/mL, human, STEMCELL Technologies) and antibiotic antimycotic solution (100x ...
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bioRxiv - Cell Biology 2023Quote: Frozen human PBMCs were purchased from STEMCELL Technologies. After thawing by immediately placing them into a 37°C water bath and transferring them into a pre-warmed RPMI1640 with 10% FBS and 1% Penicillin-Streptomycin ...
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bioRxiv - Biochemistry 2023Quote: Human peripheral blood Pan-T cells (Stemcell Technologies) were thawed and expanded following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... frozen human and cynomolgus macaques PBMCs (Stemcell Technologies, Cat# 70025.2 and iQ Biosciences ...
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bioRxiv - Cell Biology 2023Quote: Frozen human PBMCs were purchased from STEMCELL Technologies, and dead PBMCs were prepared by treating them with 70% ethanol for 30 min ...
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bioRxiv - Immunology 2023Quote: ... Human Peripheral Blood Plasma (3%; Stem Cell Technologies), 3,3‘,5-Triiodo-L-thyronine (1 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... Human Intesticult™ (StemCell Technologies, Cambridge, MA, USA) with Primocin® (20 µl of a 500X stock ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-12 (Stemcell Technologies, Vancouver, Canada), IL-15 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human Noggin (100 ng/ml, Stemcell Technologies), B-27 supplement (ThermoFisher) ...
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Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... recombinant bFGF (20 ng/mL, human, STEMCELL Technologies), recombinant EGF (20 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... RosetteSep™ Human CD8+ Enrichment Cocktail (STEMCELL Technologies) was used for cytotoxic T cells or CD4+ T Cell Enrichment Cocktail (STEM-CELL Technologies ...
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bioRxiv - Microbiology 2024Quote: Human iPSCs (SCTi003-A, STEMCELL Technologies, 200-0511) were maintained in mTeSR Plus pluripotent stem cell medium (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Recombinant Basic Fibroblast Growth Factor (STEMCELL technologies), Human Epidermal Growth Factor (PEPROTECH) ...
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bioRxiv - Cell Biology 2024Quote: ... human BDNF (10 mg/L, STEMCELL Technologies, 78058), human NT-3 (10 mg/L ...
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bioRxiv - Genetics 2024Quote: Cryopreserved human peripheral blood mononuclear cells (STEMCELL Technologies) were thawed and T cells were isolated using EasySep Buffer and the EasySep Human T Cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Genomics 2020Quote: ... Neutrophils were freshly isolated directly from whole blood by negative selection immunomagnetic purification with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies; cat. no. 19666).
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bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
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bioRxiv - Immunology 2021Quote: ... CD34+ peripheral stem cells were isolated from peripheral blood and expanded using the Complete Kit for Human Whole Blood CD34+ Cells (Stem Cell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were isolated from healthy donor-derived PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit (STEMCELL Technologies, Cat. # 17953). CD8+ T-cells were then activated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: Primary human CD4 and CD8 T cells were isolated from PBMCs by negative selection using STEMCELL EasySep isolation kits (Stemcell Technologies, 17952 and 17953), and mixed 1:1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Total CD4+ T cells were isolated by immunomagnetic negative selection using the EasySep™ Human CD4+ T Cell Isolation Kit (STEMCELL Technologies, cat. #17952) according to the manufacturer’s instructions ...