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Citations for Stemcell Technologies :
2151 - 2200 of 2339 citations for Testosterone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Single cell suspensions of splenocytes were prepared and CD4+ T cells were isolated using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies; Cat # 19852A). CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were isolated from healthy donor-derived PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit (STEMCELL Technologies, Cat. # 17953). CD8+ T-cells were then activated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Scientific ...
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bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
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bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell pellet was resuspended in 500 µL HBBS with 0.5% fatty acid-free BSA and lymphocytes were isolated by using the EasySep Mouse Streptavidin RapidSpheres isolation kit (STEMCELL Technologies, Vancouver, British Columbia, Canada) with the protocol provided ...
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bioRxiv - Bioengineering 2022Quote: ... printed constructs were incubated for 20 min in 5 mg mL-1 collagenase type I (STEMCELL Technologies) in PBS supplemented with 5% FBS and then lysed with RLT buffer ...
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bioRxiv - Bioengineering 2021Quote: ... differentiation was initiated using the stage 1 medium consisting of STEMDiff APEL base medium (STEMCELL Technologies 05275) supplemented with 6 uM CHIR99021 (Xcess Biosciences m60002) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×105 cells were seeded in 35mm dishes in duplicate in H4100 base methocult (StemCell Technologies, Inc.), supplemented with 10% FBS and the recommended volume of IMDM in the presence increasing concentrations of rigosertib ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were grown for 1 day with Stem Differentiation Neuronal induction medium (Stem Cell Technologies Catalog #05835), supplemented with the 10 μM ROCK inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... neurospheres emerged in the media and were passaged by dissociation with 1 mL Accutase (STEMCELL Technologies, 07920) for 5 minutes at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides are then stained for 1-2 days in Carmine Alum (Stem Cell Technologies; Catalog No. 07070) at RT ...
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bioRxiv - Cell Biology 2020Quote: ... patient-derived GSCs (used in Figure. 1) were cultured in Neurocult NS-A basal medium (STEMCELL technologies) supplemented with 10% BSA (STEMCELL technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... with StemSpan SFEM II containing 1% Penicillin Streptomycin and the StemSpan Myeloid Expansion Supplement II (StemCell Technologies). At day 15 ...
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bioRxiv - Molecular Biology 2023Quote: ... and plated in fresh mTeSR™1 media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Molecular Biology 2023Quote: Primed H9 hESCs were cultured on Matrigel-coated culture dishes in mTeSR™1 media (Stemcell Technologies) according to the manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: ... and plated in fresh mTeSR™1 media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Cell Biology 2023Quote: ... with the addition of 1 μg/L of human interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Bioengineering 2023Quote: ... + 5% fetal bovine serum + 1% Pen/Strep (Mediatech, MT30-002-CI) +/- 30 µM Y27632 (StemCell Technologies, 72304) was added to just cover the dissected kidneys ...
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bioRxiv - Genomics 2019Quote: CD4+ T cells were enriched from PBMCs by negative MACS selection using the EasySep™ Human CD4+ T Cell Isolation Kit (STEMCELL Technologies SARL, Grenoble, France), according to manufacturer’s instructions.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... splenic T-lymphocytes were negatively selected from total splenocytes through use of EasySep Mouse T-cell Isolation Kit (Stem Cell Technologies #19851, Vancouver, BC, Canada). Samples were validated for >90% viability and purity before subsequent use.
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bioRxiv - Microbiology 2020Quote: ... Primary human B cells were isolated by negative selection using RosetteSep Human B Cell Enrichment and EasySep Human B cell enrichment kits (Stem Cell Technologies, #15064 and #19054), according to the manufacturers’ protocols ...
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bioRxiv - Immunology 2022Quote: ... successfully transduced cells were enriched based on CD19t expression on day 11-15 using EasySep™ Human CD19 Positive Selection Kit II (Stemcell Technologies, Inc., Cat#17854), and the resulting CAR+ T cells were cryopreserved at day 15 or 17 ...
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bioRxiv - Neuroscience 2019Quote: ... splenocytes were further separated by negative selection using the EasySep T cell isolation kit designed for CD3+ T cells (Stem Cell Technologies, Vancouver, BC, Canada) following the manufacturer’s guidelines for manual separation ...
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bioRxiv - Immunology 2021Quote: ... Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies, Cat#: 19157 or 19159), or isolated from purified CD4+ or CD8+ T cells by CD45RO microbeads (see Suppl ...
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: Astrocyte precursor cells were created from the neural progenitor cells described above using the STEMdiff Astrocyte Differentiation media kit from STEMCELL Technologies (Cat No. 100-0013) following their protocol for Matrigel-coated 6-well plates [30] ...
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An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: Samples were depleted of cells of an immune origin with the RosetteSep CD45 depletion kit (depletes with antibodies for CD45, CD66, and Glycophorin A, cat. no. 15122, Stem Cell Technologies, Cambridge, MA USA) according to the protocol with SepMate - 15 tubes (cat ...
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bioRxiv - Cancer Biology 2023Quote: The first step consists in a CTC enrichment from whole blood sample by immunomagnetic negative selection using the EasySep™ Direct Human CTC Enrichment Kit (StemCell Technologies®, Vancouver, Canada) according to manufacturer instruction ...
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bioRxiv - Microbiology 2024Quote: ... Bulk CD4+ T cells were subsequently isolated from PBMCs by magnetic negative selection using an EasySep Human CD4+ T cell isolation kit (STEMCELL Technologies; per the manufacturer’s instructions). Isolated CD4+ T cells were suspended in RPMI 1640 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... H9 ES cells (at 80-90% confluency) were enzymatic detached using dispase (1 mg/ml, Stemcell Technologies, 07923). Detached cells were transferred into medium at 3:1 ratio of mTeSR1 to Neural Induction Medium (NIM ...
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bioRxiv - Developmental Biology 2020Quote: Human embryonic stem cells (hESCs; H9 line) were cultured in mTeSR™1 basal medium (Stem Cell Technologies), in 6-well plates coated with matrigel (Corning Matrigel Matrix hESC-qualified) ...
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bioRxiv - Neuroscience 2020Quote: ... hiPSC colonies were passaged 1:6 every 4 to 7 days with Gentle Cell Dissociation Reagent (STEMCELL Technologies). Genomic integrity was validated by G-band karyotyping (Cell Line Genetics ...
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bioRxiv - Bioengineering 2019Quote: ... using density gradient centrifugation on iso-osmotic medium (density: 1.077 g mL−1, Lymphoprep, Stemcell Technologies, Köln, Germany). In short ...
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bioRxiv - Developmental Biology 2019Quote: ... coated dishes (diluted 1:40 matrigel:DMEM/F12) and grown in Tesr™ E8™ medium (Stem Cell Technologies). Cells were passaged upon treatment with ReLeSR (Stem Cell Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in mTeSR1 + 1% P/S and 10 µM Rock Inhibitor (Y-27632, StemCell Technologies, #72308). A full detailed protocol for cell line maintenance can be found at www.allencell.org/sops SOP ...
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bioRxiv - Cell Biology 2019Quote: Approximately 1×106 hiPSCs were first detached in cell clumps using the gentle cell dissociation reagent (STEMCELL Technologies) and resuspended in cold PBS containing 50% of Geltrex and kept on ice ...
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bioRxiv - Neuroscience 2020Quote: ... and passed weekly at a ratio of 1:10-50 dependent on colony confluency using ReLeSR (StemCell Technologies).
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bioRxiv - Immunology 2022Quote: Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies).
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies). Before activation and at different time points thereafter ...
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bioRxiv - Microbiology 2023Quote: ... cells were differentiated in air liquid interface during 1 month using PneumaCult™-ALI Medium (Stem cell technologies) supplemented with Hydrocortisone stock solution (5 μl/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... CD34+ HSPCs were cultured at 1×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured on Matrigel-coated dishes using either mTeSR-1 or TeSR-E8 medium (Stem Cell Technologies). The medium was replaced daily until cells get 70-80% confluence ...
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bioRxiv - Cancer Biology 2022Quote: ... The organoids were passage through shear stress with cold 1 U/ml Dispase/DMEM/F12 solution (StemCell Technologies) followed by Trypsin-EDTA (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... MEF feeders were inactivated at passage 4 with chemical treatment of 1 μg/ml mitomycin-c (STEMCELL Technologies) overnight ...
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bioRxiv - Genetics 2023Quote: ... 1 million cells were cultured for 8 days in StemSpanTM SFEM II medium (Stem Cell Technologies Catalog #09605) supplemented with 1X StemSpanTM Erythroid Expansion Supplement (Stem Cell Technologies Catalog #02692).
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bioRxiv - Bioengineering 2024Quote: ... 500 and 1500 CD34+ cells were added to 1 mL of MethoCult™ H4034 Optimum medium (StemCell Technologies) and placed into one well of a 6-well plate ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T-cells were negatively isolated from PBMC by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies; average purity 93%, n=16).
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bioRxiv - Cancer Biology 2023Quote: ... T cells were then isolated using EasySep™ Human T Cell Isolation Kit and EasySep™ Magnet (Stemcell Technologies, Cat. No. 17951 and Cat. No. 18000) following manufacturer’s protocol ...