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Citations for Stemcell Technologies :
2001 - 2050 of 2339 citations for Testosterone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... After an additional 48 hours non-adhered cells were discarded and adhered T-ALL cells were separated from glioblastoma cells using EasySep Human CD3 positive Selection kit II (Stem Cell Technologies) and viability was determined by trypan blue staining (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were subsequently isolated from the PBMC population by immunomagnetic negative selective with the EasySep Human T Cell Isolation kit (StemCell Technologies, Canada). Isolated T cells with >95% viability and >95% purity were either cryopreserved or directly cultured for subsequent experiments ...
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bioRxiv - Genetics 2023Quote: Peripheral blood mononuclear cells were isolated by standard Ficoll gradient centrifugation and classical monocytes isolated using EasySep™ Human Monocyte Isolation Kit from Stemcell Technologies according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Human CD34+ cells were purified from fresh BM cells using MicroBead kit (Miltenyi Biotech 130-046-702) and subsequently cryopreserved in CryoStor® CS5 (STEMCELL Technologies).
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bioRxiv - Bioengineering 2023Quote: ... and mouse T cells were purified from mouse splenocytes using a negative selection EasySep™ Mouse T Cell Isolation Kit (STEMCELL Technologies). Mouse splenocytes were prepared from spleens collected from 8-18 weeks old male BL6 mice purchased from Orient bio ...
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bioRxiv - Immunology 2023Quote: ... CD14+CD16-monocytes were isolated from peripheral blood mononuclear cells using immunomagnetic negative selection (EasySep Human Monocyte Isolation Kit, Stem Cell Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
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bioRxiv - Immunology 2023Quote: CD63+ DCs or CD63- control DCs isolated from the LNs of Balb/c mice were sorted by FACS as described previously and cultured with naïve CD4+ T cells magnetically isolated from Fox3-GFP+ mice (C57Bl/6 background) using EasySep Mouse Naïve Cd4+ T cell isolation kit (Stemcell Technologies, Cat# 19765) at a ratio of 1:5 DC:T cell for 5 days in RPMI containing 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Human CD34+ cells were isolated from cord blood using EasySep™ human cord blood CD34 positive selection kit II (STEMCELL Technologies #17896), which were then cryopreserved in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
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bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
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bioRxiv - Cancer Biology 2023Quote: ... apoptotic and dead cells were removed by immunomagnetic cell separation using the Annexin Dead Cell Removal Kit (StemCell Technologies, cat. no. 17899) and EasySep Magnet (StemCell Technologies ...
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bioRxiv - Immunology 2023Quote: ... Human blood neutrophils from healthy controls were isolated using negative sorting with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies 19666), loaded with DCFDA ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from pooled splenic cell suspensions by magnetic negative selection using EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Self-organizing cerebral organoids were derived from induced pluripotent stem cells (iPSC) using the STEMdiff™ Cerebral Organoid Kit developed by STEMCELL Technologies™ (catalogue # 08570) ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells that passed through 70-uM cell strainer were purified with EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies Inc). For T-cell proliferation assays ...
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bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T-cells were isolated from the thawed CD14-population on Day 6 using the EasySep Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies Cat. #19258) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from human peripheral blood using EasySepTM Human CD8+ T Cell Isolation Kit (Stemcell Technologies, Vancouver, BC, Canada) and stained with 10 μM CellTraceTM Violet Cell Proliferation Kit (VPD ...
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bioRxiv - Genetics 2023Quote: ... cerebral organoids were derived from hiPSC of n=3 healthy donors using the STEMdiff™ Cerebral Organoid Kit (StemCell Technologies, #08570, #08571) according to the manufacturer’s instructions and collected on days 21 ...
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bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
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bioRxiv - Immunology 2024Quote: The enzymatic activity of RALDH2 in BMDCs and MLN cells was assesses using the ALDEFLUOR Kit (#01700, STEMCELL Technologies, Vancouver, BC, Canada). DCs were pre-gated as CD11c+/MHC IIhigh cells and RALDH2 activity was detected by a flow cytometric analysis.
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bioRxiv - Immunology 2024Quote: For further examination of neutrophil granulocytes we isolated granulocytes with the EasySep® Direct Human Neutrophil Isolation Kit (Stemcell Technologies, Cat# 19666), following the instructions provided by the manufacturer ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells were isolated from whole blood using a density based negative selection kit according to the manufacturer’s instructions (RosetteSep Human NK Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation ...
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bioRxiv - Bioengineering 2024Quote: ... CD56+CD3- NK cells were isolated from the PBMC population using the EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). NK cells were frozen at 2.5e6 or 5E6 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... T cells from the transgenic mouse splenocytes were enriched by magnetic isolation using the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies). T cells were centrifuged and resuspended in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... the spheres were chemically dissociated after seven days of plating, using the NeuroCult Chemical Dissociation Kit (mouse, #05707) from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were seeded at a 1:6 ratio and routinely passaged with Dispase (Stemcell Technologies).
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bioRxiv - Bioengineering 2021Quote: ... Cells were counted using a haemocytometer and seeded in mTeSR™1 (STEMCELL Technologies, 85850) and a density of 5×105 cells/ml as a 1ml/well solution in a 12-well culture dish with 10uM Rock inhibitor Y27632 (ATCC® ACS-3030™) ...
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bioRxiv - Neuroscience 2020Quote: ... hPSCs were dissociated to single cells in Dispase (1 U/mL, Catalog #07923, STEMCELL Technologies), and quickly reaggregated using low-cell-adhesion-coated 96-well plates with V-bottomed conical wells (Corning ...
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bioRxiv - Genetics 2022Quote: ... and routinely passaged 1:10 every 5 days using Gentle Cell Dissociation Reagent (Stemcell Technologies).
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bioRxiv - Immunology 2021Quote: ... 10,000 sorted cells were plated in 1 ml MethoCultTM M3630 (STEMCELL Technologies, Catalogue no.-03630) containing 2% PenStrep ...
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bioRxiv - Neuroscience 2022Quote: ... IPSCs were cleaned and 1/3 dish was gently dissociated with dispase (Stemcell Technologies # 07923) for 12-15 minutes at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... with 1:800 dilution of membrane permeant NeuroFluor™NeuO probe (cat# 01801, StemCell technologies) to detect neurons and 1:500 dilution of cell permeable DNA dye Vybrant® DyeCycle™ Ruby Stain (cat# V10309 ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with normal rat serum (50 µl/ 1×108 via.cells, Stem Cell Technologies and Jackson ImmunoResearch Laboratories ...
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bioRxiv - Bioengineering 2020Quote: ... we then topped the organoids with 1 mL of fresh BrainPhys neuronal medium (Stemcell Technologies) and incubated them in a 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Multiple subclones of iPS cell lines were expanded on mTeSR™1 (Stem Cell Technologies) and Matrigel (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded (between 5×103 and 1×104) in methylcellulose (Methocult H4100, StemCell Technologies) in triplicate ...
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bioRxiv - Cell Biology 2020Quote: ... replaced with 1 ml of BrainPhys Neuronal Medium supplemented with N2 Supplement-A (StemCell Technologies) and B27 supplement minus vitamin A (N2 / B27 BrainPhys Neuronal Medium) ...
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bioRxiv - Physiology 2022Quote: ... Selected cells (1 × 105 cells/ml) were expanded in StemSpan H3000 media (StemCell Technologies Inc) supplemented with StemSpam CC100 (StemCell Technologies Inc ...
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bioRxiv - Neuroscience 2022Quote: ... and were grown for 1 day with STEMdiff Neuronal induction medium (STEMCELL Technologies catalog # 05835), supplemented with 10 μM ROCK inhibitor (Y-27632 dihydrochloride ...
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bioRxiv - Neuroscience 2023Quote: ... EBs were treated for 1 hour with neural rosette selection reagent (05832,Stem Cell Technologies) prior to collection and replating into matrigel-coated 12-well plastic culture plates to establish passage 0 (p0 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×103 cells were resuspended in 1 ml of complete MammoCultTM Basal Medium (Stemcell Tech) and seeded in ultralow attachment plates ...
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bioRxiv - Neuroscience 2023Quote: ... and CD11B-FITC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60040FI) antibodies for 15 minutes in the dark at 4C ...
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bioRxiv - Immunology 2023Quote: ... the cell pellet was resuspended in 1 mL of Cryostor CS10 (Stemcell Technologies Catalog #07930) and transferred to a cryotube ...
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bioRxiv - Neuroscience 2023Quote: ... and CD34-PE (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60119PE) antibodies for 15 minutes in the dark at 4C ...
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bioRxiv - Developmental Biology 2023Quote: ... Our hiPSC lines were cultivated under sterile conditions in mTeSR-1 (Stem Cell Technologies, Vancouver), changed daily ...
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bioRxiv - Neuroscience 2023Quote: ... and routinely passaged 1:10 every 5 days using Gentle Cell Dissociation Reagent (StemCell Technologies).
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bioRxiv - Neuroscience 2024Quote: ... A full-media change with StemFlex supplemented with CloneRTM (Stemcell Technologies; 05888; 1:10 dilution) was performed once ...
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bioRxiv - Neuroscience 2023Quote: At day -1 hiPSCs expanded in 10cm dishes were dissociated with accutase (StemCell Technologies, #07920), washed and pelleted with a 1:4 ratio of accutase to DMEM for 5 min at 1000 rcf ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated from WT mice by magnetic bead negative selection using EasySep Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 media supplemented with 10% FBS ...