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Citations for Stemcell Technologies :
2001 - 2050 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The remaining pellet was gently resuspended in enteric glial cell media supplemented with 1x CloneR (Stem Cell Technologies, 5889) and 50ug/mL primocin (Invivogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended in 4 ml Red Blood Cell Lysis buffer (Ammonium Chloride solution, Stemcell Technology, #07800) on ice for 2 min and washed with 6 mL DME/F-12 medium at 450 g for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged twice a week after reaching approximately 80% confluency using PBS/EDTA or ReLeSRTM (Stem Cell Technologies) as a dissociation reagent ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from naïve splenocytes using the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies) and plated at 3x105 cells per well in a flat bottom 96 well plate ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR; Stem Cell Technologies, 05872). Cells were checked for mycoplasma contamination every two weeks ...
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured in the presence of TGFβ and BMP inhibitors (LDN193189, #72142, Stem Cell Technologies; SB431542, #1614, Tocris) to trigger cortical precursors for cortical neuron differentiation ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 300g for 5 mins and RBC lysed using Ammonium Chloride Lysis Buffer (Stem Cell Technologies) as per manufacturer protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were cultured in collagen-I gels in the ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies, Cat# 10981) with the addition of Human Recombinant Interleukin 2 (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... The OSCs were produced in multiple batches and cryopreserved in vials of 120,000 to 150,000 live cells and stored in liquid nitrogen in CryoStor CS10 Cell Freezing Medium (StemCell Technologies). For the purposes of this study ...
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bioRxiv - Immunology 2024Quote: ... Total CD8+ T cells were isolated by magnetic sorting (EasySep™ Mouse CD8+ T Cell Isolation Kit, StemCell Technologies), followed by tetramer E7 and TRM marker staining ...
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bioRxiv - Genetics 2024Quote: ... Cells were subjected to a 20-day protocol for hepatocyte differentiation using the STEMdiffTM hepatocyte kit (Stem Cell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... naïve (CD44loCD62Lhi) CD8+ T cells were purified using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit (Stemcell Technologies) and stimulated with plate-bound α-CD3 (5 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: Naïve (CD44loCD62Lhi) CD8+ T cells were purified using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Peripheral blood mononuclear cells were cultured for 8 days in erythroblast expansion medium (StemSpan™ SFEM medium (Stem Cell Technologies) supplemented with Epo (2 U/mL) ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using SepMate-50 Isolation tubes (Stem Cell Technologies, Vancouver, Canada) per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (Stem Cell Technologies). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were grown on either Corning or Eppendorf tissue culture-treated plates coated with Vitronectin XF (Stem Cell Technologies #7180) and passaged using either TrypLE Express Enzyme (Gibco ...
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bioRxiv - Bioengineering 2019Quote: ASCs on their 3rd passage were plated onto a 12 well plate at 20,000 cells per well in 1.5 ml of MesenCult MSC medium (Stem Cell Technologies) and allowed to grow for 2 days ...
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bioRxiv - Genetics 2021Quote: ... cells were cultured in R10 media additionally supplemented with 16 ng/mL of hepatocyte growth factor (HGF, Stem Cell Technologies) to preserve viability and support robust growth (Datta et al. ...
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bioRxiv - Immunology 2021Quote: 0.25-0.5 million non-parenchymal cells were used with the EasySepTM Mouse T cell Isolation Kit (Stemcell Technologies, Cat.# 19851) for negative selection of T cells ...
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bioRxiv - Cancer Biology 2020Quote: ... NK cells were enriched from healthy donor PBMCs using the EasySep NK cell negative selection kit (StemCell Technologies, Cambridge, MA) and confirmed to be >95% purity using flow cytometry.
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bioRxiv - Biochemistry 2020Quote: CD8+ or total T cells were isolated from spleens with an EasySep(tm) T Cell Isolation Kit according to the manufacturer’s instructions (STEMCELL Technologies).
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bioRxiv - Neuroscience 2020Quote: ... Cells were spun down (120 × g) for 3 min and resuspended in the required amount of mTeSR (Stem Cell Technologies) + Rock Inhibitor (RI) ...
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bioRxiv - Neuroscience 2021Quote: ... and spheroid formation was performed by transferring 1.5 × 106 iPSCs (5,000 cells/microwell) into AggreWell 800 plates (Stem Cell Technologies) in spheroid medium (50% DMEM-F12 Glutamax ...
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bioRxiv - Neuroscience 2021Quote: ... and plated in single cells at 50,000 cells/cm2 in mTeSR Plus media supplemented with 10 µM Y-27632 (STEMCELL Technologies) on tissue culture plates coated with 336.67 µg/mL Growth Factor Reduced matrigel (Corning) ...
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bioRxiv - Cancer Biology 2022Quote: ... Myeloma cells were selected from total bone marrow mononuclear cells using anti-CD138 magnetic bead enrichment (StemCell Technologies, Cat #17887) prior to cryopreservation and use ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells were isolated from blood and plated at 50,000 cells per ml in MethoCult 4034 (Stemcell Technologies). After 14 days in culture ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1mM EDTA-2Na).Lineage negative (Lin−) cells were enriched using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (StemCell technologies) and stained with fluorescent antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... mice were injected with 5-15×105 NK cells purified using the NK cell enrichment negative selection kit (STEMCELL Technologies).
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bioRxiv - Immunology 2020Quote: ... CD3+ T cells were isolated from whole splenocytes using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies, #19851), as per the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2019Quote: HSCs were plated at 500 cells per 6cm dish filled with 1ml of either Methocult-Enriched (H4435, Stem Cell Technologies) supplemented with recombinant cytokines including EPO ...
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bioRxiv - Developmental Biology 2021Quote: ... The mixture of MethoCult and cells was vortexed and transferred with a blunt end needle syringe (Stem Cell Technologies, 28110) into meniscus-free SmartDish plates (6-well-plate ...
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bioRxiv - Immunology 2020Quote: CD8 T cells were purified from spleens by negative selection using EasySep Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... 600000 single stem cells were seeded per well (500 cells/microwell) in aggregation media with 50 μM Y27632 (STEMCELL technologies). Mesoderm induction and sprouting was induced directly in the AggreWell™400 plates by carefully changing the media with a p1000 pipette making sure to not disturb the cell aggregates in the microwells ...
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bioRxiv - Biophysics 2020Quote: ... The cells were resuspended in 1 mL of medium contained 1x Stem Span Cytokine Cocktail CC100 (Stem Cell Technologies, Canada) in 24-well plate ...
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bioRxiv - Immunology 2021Quote: ... Untouched CD8+ T cells were magnetically isolated using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, Grenoble, France) or MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend ...
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bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were plated at concentrations of 1,000 cells per plate in duplicate on methylcellulose medium (MethoCult GF M3434, STEMCELL Technologies). Colonies were scored manually after 8-10 days ...
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bioRxiv - Cancer Biology 2022Quote: ... Low-density (1.077g/cc) mono-nuclear cells were obtained from AML BM using Ficoll density gradient centrifugation (Stem cell technologies).
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bioRxiv - Neuroscience 2023Quote: ... We passaged cells when the confluency of culture reached about 85% by using Gentle Cell Dissociation Medium (Stemcell Technologies, #07174) and ROCK inhibitor (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Frozen cells were thawed at 37°C and treated using the ErythroClear™ Red Blood Cell Depletion Kit (STEMCELL Technologies). Cells were constructed into barcoded single-cell cDNA libraries and sequenced as described above.
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood B cells were isolated via negative selection using RosetteSep immunodensity-based cell separation (Stemcell Technologies, Vancouver, BC, Canada). The purity of CLL/ B cells was analyzed by flow cytometry and revealed that ≥ 90% cells co-expressed CD5/CD19.
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bioRxiv - Cancer Biology 2024Quote: ... CD138 positive plasma cells were purified from available matching BM aspirates using immunomagnetic bead selection (EasySep™; Stem Cell Technologies). Total RNA from 7 of these patients was extracted from CD138+ cells using the miRNeasy Mini Kit (Qiagen GmbH ...
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bioRxiv - Cancer Biology 2024Quote: Both WT and MT iPS cells were maintained in mTeSRTM1 medium with 1x Supplement media (Stem Cell Technologies, Catalog #85850) and supplemented with 10 μM Y27632 (Stemgent ...
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bioRxiv - Immunology 2024Quote: ... Tregs were seeded at an approximate density of 1.2 million cells/cm2 in ImmunoCult-XF T cell expansion medium (STEMCELL Technologies) supplemented with 50 μM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Murine splenic B cells were isolated by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies, #19854A) as specified by the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Twenty-four hours later 10/50/500 non irradiated CD34+ cells or 1,500/7,500/15,000 irradiated (3Gy) CD34+ cells per well were plated in 100 μl MyeloCult H5100 medium (STEM CELL technologies). Once a week half medium replacement was done ...
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bioRxiv - Cancer Biology 2023Quote: ... ∼2,000 CD34+Lin-CD38-CD90loCD45RA-EPCR+ cells were cultured on tissue culture plates containing StemSpan II media (Stem Cell Technologies) supplemented with 50ng/ml recombinant human SCF (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... Stem cell culture was done using a commercially available media kit (mTeSR Plus; Cat No. 100-0276, STEM CELL Technologies) following the manufacturer’s published protocol [32] ...
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bioRxiv - Cell Biology 2023Quote: ... sorted 250 cells/well were plated in a 6 well plate of MegaCult C Medium plus Lipids (Stem Cell Technologies) with rhEPO (recombinant human erythropoietin) ...