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Citations for Stemcell Technologies :
2251 - 2300 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... in TeSR-E6 medium (Stem Cell Technologies, 05946) for 5 days ...
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bioRxiv - Immunology 2023Quote: ... as per the manufacturer’s protocol (Stem Cell Technologies). Specifically ...
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bioRxiv - Cell Biology 2023Quote: ... equal volume complete mTeSR media (Stem Cell Technologies) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... using mTeSR1 stem cell media (Stemcell Tech. Inc.). The efficiency of RB1 or P53 knockdown and production of cMYC or cMYC (T58A ...
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bioRxiv - Neuroscience 2023Quote: ... Then cells were passaged using ReLeSRTM (STEMCELL Technologies).
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bioRxiv - Molecular Biology 2023Quote: ... Using the EasySep protocol from STEM cell technologies, these cells were sorted into CD14+ monocytes ...
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bioRxiv - Cell Biology 2023Quote: ... which consisted of StemSpan SFEM (Stem Cell Technologies) supplemented with 50 ng/ml SCF ...
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bioRxiv - Immunology 2023Quote: ... Cells were grown in mTeSR1 (STEMCELL Technologies, 85850) with penicillin-streptomycin (0.5% v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged using Accutase (StemCell Technologies, 07920) and plated at a density of 5,200 cells/cm2 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were dissociated with accutase (StemCell Technologies; 07920) and replated into a 96-well plate at a 1:5 dilution (accounting for 1:5 reduction in surface area between 24 to 96-well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 μg/ml heparin (Stem cell technologies; #07980), 20 ng/ml Animal-Free Recombinant Human EGF (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were maintained in either mTESR1 (StemCell Technologies) or TeSR-AOF (StemCell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with BIT serum substitute (Stem Cell Technologies) and cytokines (SCF ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10 μM Y27632 (Stem Cell Technologies) for the first 24 hours ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: The ALDEFLUORTM Assay kit (Stem Cell Technologies, #01700) was used to determine ALDH activity according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 1 µM CHIR99021 (Stem Cell Technologies), 9 ng/mL Activin A ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were maintained in mTeSR Plus (STEMCELL Technologies) at 37°C and 5% CO2 with media being changed every other day and cultures being passaged at 80% confluency ...
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bioRxiv - Microbiology 2024Quote: ... and 50 ml SepMate tubes (STEM CELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... where randomly grouped and 2 x 106 naïve Ly5.2+CD8+CD44loCD62Lhi OT-I T cells [purified by FACS or using the EasySep™ murine naïve CD8+ T cell isolation kit (Stemcell Technologies)] were injected intravenously ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were manually counted on a hemocytometer using Trypan blue and plated in methylcellulose media (M3434 or M3334, Stem Cell Technologies) at a density of 5×105 live cells/ml (in M3434 ...
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bioRxiv - Developmental Biology 2020Quote: ... YS were digested with collagenase I to dissociate the cells into single cell suspension and plated in methylcellulose medium (media M34334, StemCell Technologies) containing cytokines (SCF ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies) or StemMACS HSC Expansion Media (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were initially dissociated into single cell suspensions by accutase treatment (Stemcell Technologies, 07920, 30-60 min, 37 °C, 5% CO2) and then fixed and permeabilized using fixation/permeabilization buffers (Foxp3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Immunology 2022Quote: ... NK cells were isolated from buffy coats from healthy donors using the RosetteSep NK cell enrichment kit (STEMCELL Technologies, MA, USA) and stimulated with rhIL-15 (1ng/ml ...
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bioRxiv - Immunology 2021Quote: Bone marrow colony forming assay was performed by plating 104 or 5×104 cells on methylcellulose (MethoCult M3434, Stem Cell Technologies). GEMM (Granulocyte/Erythrocyte/Macrophage/Megakaryocyte) ...
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bioRxiv - Physiology 2020Quote: ... Single-cell suspensions were plated at a density of 1.2 × 105 cells/cm2 and cultured overnight in mTeSR medium supplemented with 10 μM Y-27632 dihydrochloride (Stem Cell Technologies). Once the monolayer reached approximately 80% confluence (usually the following day) ...
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bioRxiv - Immunology 2019Quote: ... were thawed at 37°C and dead cells were removed through Annexin V beads-mediated selection (EasySep Dead Cell Removal Annexin V Kit, Stemcell technology). CD4+ T cells were further isolated through beads-mediated negative selection (EasySep Human CD4+ T cell Isolation Kit ...
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bioRxiv - Cell Biology 2019Quote: ... 100 GFP+ cells from transduced MPP4s or 200 GFP+ cells from transduced LT-HSCs were plated in Methocult GF M3434 (StemCell Technologies) and cultured at 37°C and 5% CO2 ...
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bioRxiv - Genomics 2020Quote: ... Lymph nodes were harvested from a 6-week-old GFP OT-1 mouse and CD8 T cells were isolated using an EasySep mouse CD8 T cell negative selection kit (StemCell Technologies). 14 days post tumor injection ...
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bioRxiv - Bioengineering 2021Quote: ... A 3x103 cell fraction of non-transduced and transduced Lin-cells were transferred into 3 ml methylcellulose-based medium with mouse cytokines optimized for hematopoietic progenitor cell differentiation (StemCell Technologies) and plated at 1 mL/well for a CFU assay ...
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bioRxiv - Bioengineering 2020Quote: ... Clear supernatant was removed and intestinal pieces were resuspended in 25 ml of Gentle Cell Dissociation Reagent (Stem Cell Technologies, 07174). Tubes were incubated at room temperature on a rocking platform for 20 minutes ...
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bioRxiv - Neuroscience 2019Quote: Neurons were differentiated from the WA09 (H9) pluripotent stem cell line maintained in mTesr1 medium (Stem Cell Technologies, Vancouver, BC, Canada). Stem cells were plated in 6-well plates coated in Matrigel in feeder-free conditions with daily media changes and split 1:6 one day before reaching confluency to ensure maintenance of pluripotency ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were spun at 350xg for 10min and the red blood cells were lysed with 5ml Ammonium chloride (StemCell Technologies) per 50ml tube for 5min ...
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bioRxiv - Cell Biology 2021Quote: GMPs were sorted as described earlier from WT (C57Bl6/J) and 50K cells were cultured in 0.5mls of StemSpanTMSFEMII (Stem cell technologies) supplemented with 1% L-Glutamine and Penicillin/Streptomycin in addition to mouse recombinant cytokines ...
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bioRxiv - Bioengineering 2020Quote: ... Cryopreserved PBMCs were quickly thawed and resuspended at a concentration of 4×106 cells/ml in Eagle's minimal essential medium (EMEM; Stem Cell Technologies; supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... media was removed from HNAE cells apical side (airlift) and provided with Pneumacult ALI basal media from HNAE cells basolateral side (Stemcell technologies). HAE cells were fully differentiated and ready to use after 28 days post-airlift ...
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bioRxiv - Systems Biology 2022Quote: ... and total peripheral blood mononuclear cells (PBMCs) were isolated by density centrifugation according to the manufacturers protocol (Lymphoprep, Stem Cell Technologies). Samples were cryopreserved at a concentration of 107 cells per milliliter in a solution of 90% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... total B cells from the spleens and lymph nodes were isolated using the Negative Selection B cell isolation kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed in IMDM+20%FCS and plated into 1.4 mL of cytokine-rich methylcellulose (Methocult H4435, Stem Cell Technologies). Colonies were scored 14 days later under an inverted microscope ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were dissociated into a single cell suspension with TrypLE and replated on matrigel-coated plates at a density of 40,000 cells/cm2 in mTesR medium (Stem Cell Technologies). Cells were allowed to propagate with a daily change of medium for 2 days ...
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bioRxiv - Developmental Biology 2022Quote: ... they were specified into a hiPSC-CM/stromal cell mixture using RPMI B27-containing 5 µM IWP-4 (Stem Cell Technologies) followed by another 7 days of RPMI B27+ (RPMI1640 GlutaMAX + 2% B27 supplement with insulin ...
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bioRxiv - Neuroscience 2022Quote: NPCs were passaged and plated at 50,000 cells/cm2 in STEMdiff™ Forebrain Neuron Differentiation Media (Stem Cell Tech cat#08600) on Corning® Matrigel® coated tissue culture treated plates and transduced with a NEUROG2 lentivirus (GeneCopoeia cat#LPP-T7381-Lv105-A00-S ...
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bioRxiv - Bioengineering 2019Quote: ... Bulk CD3+ T cells or an isolated CD3+CD8+ T cell subset were extracted from whole blood using negative selection methods (RosetteSep, StemCell Technologies) and cultured in ImmunoCult-XF T cell Expansion Medium (StemCell Technologies) ...
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bioRxiv - Neuroscience 2019Quote: hESCs were dissociated into single cells using Accutase and 4.5×106 cells were seeded into AggreWell800 plates (STEMCELL Technologies, Vancouver, Canada) to form neural aggregates in STEMdiff Neural Induction Medium (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... were generated from confluent iPSC cultures by dissociating them to single cells and seeding them at a density of ∼4 × 106 cells per well of Aggrewell 800 6-well plates (StemCell Technologies), equating to ∼2200 cells per EB ...
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bioRxiv - Immunology 2020Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymoh nodes using CD19 Microbeads (Miltenyi) or Mouse B cell Isolation Kit (Stemcell Technologies). B cells were stimulated with LPS (10 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... at 37℃ for 10 min and then were seeded on to a vitronectin-coated six-well plate at 3×105 cell/well in mTeSR1 supplemented with 5μM ROCK inhibitor (Y-27632, Stem Cell Technology) for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... To begin the protocol cells were washed twice and incubated in phase I medium comprised of APELII medium (Stem Cell Technologies) supplemented with BMP-4 (Thermo Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve CD4+ T cells were then enriched from total CD4+ T cells through immuno-magnetic negative selection with the EasySep™ Naïve CD4+ T cell isolation kit (STEMCELL Technologies). Naïve CD4+ T cell post-isolation purity (CD4+CD45RA+CD45RO- ...
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bioRxiv - Immunology 2020Quote: ... splenocytes were first enriched for OT-II CD4+ T cells using EasySep™ mouse CD4+ T cell isolation kit (StemCell Technologies), incubated with FcR block and stained with the following fluorescence-conjugated antibodies ...