Labshake search
Citations for Stemcell Technologies :
2001 - 2050 of 4776 citations for Killer cell immunoglobulin like receptor 2DL2 KIR2DL2 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... PBMCs were cultured at 37°C under 5% CO2 for 5 days in ImmunoCult™-XF T Cell Expansion Medium (STEM CELL Technologies) supplemented with 30 ng/mL human recombinant IL-2 (Peprotech ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were immediately processed to isolate CD4+ T cells by negative selection using the EasySep Human CD4+ T Cell Isolation Cocktail (StemCell Technologies, Cat. #17952) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
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bioRxiv - Immunology 2023Quote: ... Primary CD8+ and CD4+ T cells were isolated by negative selection through either RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) or RosetteSepTM Human CD4+ T Cell Enrichment Cocktail (StemCell technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... The cryopreserved CD34+ cells of the CML patients and the normal donor were thawed and cultured in CD34+ cell expansion medium constituting StemSpan™ SFEM II medium (Stem Cell Technologies) supplemented with CD34+ Expansion Supplement and UM729 (Stem Cell Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... and subplated at 25x104 cells/ml on dishes coated with 50 ug/ml Poly-L-ornithine in BrainPhys neuronal medium (Stem cell technologies, USA) containing N2 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The middle layer containing PBMCs were then enriched for primary human NK cells (hNK) by negative selection using the EasySep Human NK Cell Enrichment Kit (StemCell Technologies, Cat# 19055), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Neuroscience 2023Quote: ... 3 million iPSCs per cell line were plated into 1 well of an AggreWell™800 24-well plate (Stem Cell Technologies, 34811) with 2 mL STEMdiff™ Neural Induction Medium + SMADi + 10 µM Y-27632 ...
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bioRxiv - Neuroscience 2023Quote: ... with a cell density of ∼12,000 cells per microwell in an EB formation medium containing neural induction medium (StemCell Technologies, Catalog No. 05835) supplemented with 100 nM of LDN-193189 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... A crude live cell mixture was obtained by filtration and negative selection using Easy Eights EasySep Magnet and Dead Cell Removal kit (Stemcell Technologies, Catalog # 17899). Cells were further en-riched with positive CD90.2 selection (Stemcell Technologies ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from single-cell suspensions from spleen tissue by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (# 19852, StemCell Technologies, Cambridge, MA) and differentiated into different T helper (Th ...
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bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Primary NK cells were isolated from PBMCs by immunomagnetic negative selection using EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies Canada, Inc.) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: Cell were dissociated from large airways or distal lung lobe regions and expanded in basal cell media containing PneumaCult™-Ex Plus medium (Stem Cell Tech) containing 0.5 μM DMH-1 (Tocris #4126) ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2024Quote: ... WTC-11 hiPSC cells (Gladstone Institute of Cardiovascular Disease, UCSF)25,26 were maintained in mTeSR Plus medium with supplement (Stem Cell Technologies, Cat.#05825) and cultured on Vitronectin XF (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... These batch were collected in 50mL Cryostore bags and frozen in a final 50 × 106 cells/mL cell density for cryopreservation using CS10 Freeze Medium (STEMCELL Technologies; cat#07930). Once CAR T cells had been harvested in CS10 Freeze Medium ...
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bioRxiv - Developmental Biology 2024Quote: ... the cells were plated in 6-well plates using mTeSR media supplemented with 10 µM Y-27632 (Stem Cell Technologies, catalog no. 72304) for 48 hours ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: Mouse Intestinal Organoids were incubated in 24-well cell culture plates at 37 °C under 5% CO2 in IntestiCult Complete Organoid Medium (#06005, Stem Cell Technologies, Canada) containing CHIR-99021 (3 μM final concentration ...
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bioRxiv - Molecular Biology 2024Quote: CD34+ cells were isolated from ficolled bone marrow mononuclear cells using the EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, 17856) according to the manufacturer’s instructions in PBS containing 2% fetal bovine serum and 1 mM EDTA ...
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bioRxiv - Immunology 2024Quote: ... splenic murine t cells were isolated from C57BL/6 mice using EasySep™ Mouse T Cell Isolation Kit (STEMCELL Technologies; Cat. No. 19851). Glioma cells were labeled with Cr51 (0.05 mCi ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 100 U ml−1 IL-2 in the presence of ImmunoCult™ CD3/CD28/CD2 T cell activator (Stem Cell Technologies # 10970) in 96 well plates overnight (150 ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 20ng/mL recombinant human IL-2 and 7μL/mL of ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stem Cell Technologies, 10970) every week.
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bioRxiv - Cell Biology 2024Quote: ... NGN2 and puromycin resistance expression was induced as follows: Seven million cells were passaged into pre-differentiation media containing Rock inhibitor (Y-27632, Dihydrochloride, Stem Cell Technologies 72304) and doxycycline (Sigma D3447 ...
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bioRxiv - Developmental Biology 2024Quote: ... the tissues were dissociated into single cell suspension using NeuroCult enzymatic dissociation kit for adult mouse and rat CNS tissue (Stem Cell Technologies 05715) and plated on ultra-low attachment 6-well plates (Corning 3471 ...
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bioRxiv - Bioengineering 2024Quote: ... (i) Dextran-coated MNPs and anti-CD4 antibodies (EasySep Human CD4 Positive Selection Kit II, STEMCELL Technologies Canada Inc.) were incubated at a 1:1 ratio over night at 3 – 8 °C ...
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bioRxiv - Cell Biology 2020Quote: ... CD34+ cells were enriched by EasySep™ from the light-density fraction of Lymphoprep™ or RosetteSep™-depleted CD11b+;CD3+;CD19+ cells (STEMCELL Technologies) and then used after cryopreservation in DMSO and fetal bovine serum (FBS ...
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bioRxiv - Synthetic Biology 2022Quote: Primary CD4+ and CD8+ T cells were isolated from blood of anonymous donors by negative selection using the Human CD4+ T cell isolation kit and Human CD8+ T cell isolation kit (STEMCELL Technologies #17952 and #17953). T cells were cryopreserved in RPMI1640 (UCSF cell culture core ...
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bioRxiv - Neuroscience 2021Quote: ... and plated at 3 × 106 cells/well to allow the embryoid body (EB) formation in Neural Induction Media (NIM) + 10 μM Y-27632 (Stem Cell Technologies, Cat.No. 72308). They were allowed to attach for at least overnight ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were pelleted by centrifugation at 300 g for 10 min and resuspended at 108 cells/mL in EasySep™ buffer and B lineage cells were isolated using an EasySep™ mouse B cell negative isolation kit (StemCell Technologies, #19854). For serum ...
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bioRxiv - Neuroscience 2022Quote: A single cell suspension of hiNCs and hiACs was prepared by AccutaseTM dissociation and washed once with DMEM/F12 (Stem Cell Tech cat#36254) to remove debris ...
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bioRxiv - Neuroscience 2022Quote: iPSC derived astrocytic cells (hiAC) were differentiated from NPCs by small molecule differentiation in STEMdiff™ Astrocyte Differentiation Media (Stem Cell Tech #cat100-0013) on Corning® Matrigel® coated tissue culture treated plates ...
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bioRxiv - Cell Biology 2020Quote: ... After centrifugation PBS was aspirated and the cell pellet was tapped loose and resuspended in mTeSR™-1 spin-EB medium consisting of mTeSR™-1 (Stem Cell Technologies), supplemented with 1 mM Rock-inhibitor (Y27632 ...
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bioRxiv - Microbiology 2021Quote: ... the isolated intestinal tissue was cut into ∼3-to 5-mm pieces and incubated in Gentle Cell Dissociation Reagent (Stem Cell Technologies, Vancouver, Canada). After removal of the Gentle Cell Dissociation Reagent ...
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bioRxiv - Immunology 2020Quote: CD4+ and CD8+ T cell subsets were depleted from PBMC using magnetic bead-based CD4 and CD8 negative selection kits (Stem Cell Technologies, Vancouver, Canada). The cell separations were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
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bioRxiv - Immunology 2020Quote: ... Each antibody clone and lot was titrated to optimal staining concentrations using primary murine samples with all appropriate positive and negative controls: polyclonal murine CD8 T cells purified by positive selection kit (Stem Cell Technologies, Vancouver, Canada) stimulated with PMA/Ionomycin via eBioscience Cell Stimulation Cocktail (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... B cells were enriched by negative selection using EasySep mouse B cell enrichment kit as per manufacturer’s instructions (StemCell Technologies Inc., Tukwila, WA, USA). Enriched B cells were then resuspended in FACS buffer and stained and sorted as described previously(Carbonetti et al. ...
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bioRxiv - Bioengineering 2022Quote: ... CD4+ T cells were isolated from the spleen of C57/BL6 mice using the CD4 T cell isolation kit from Stemcell Technologies (# 19852; Cologne, Germany) and subsequently naïve T helper cells (CD3+ ...
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bioRxiv - Molecular Biology 2024Quote: ... The assay measures the number of colonies formed in the myeloid-defined media after two weeks in culture using the stem vision plate analyzer (Stem Cell Vision, Stem Cell Technologies, Vancouver, Canada).
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bioRxiv - Neuroscience 2024Quote: ... was rapidly dissected in ice cold buffer and neural progenitor cells were harvested by centrifugation (according to (41)) and cultured in NeurocultTM proliferation media (Stem Cell Technologies, Cambridge UK) containing recombinant human EGF according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells are resuspended in N2B27 medium supplemented with ROCK inhibitor and seeded at a density of approximately 75 cells per microwell in 400 µm AggreWell plates (Stem Cell Technology, cat# 34425). Once cell structures appear solid ...
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bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
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bioRxiv - Cell Biology 2024Quote: ... we performed a Cut and Run/Q-PCR (C&R/Q-PCR) experiment of 5×105 untreated cells and 5×105 cells treated for 30 minutes with recombinant human INFγ (STEMCELL Technologies, Vancouver, BC, Canada). For the C&R procedure ...
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bioRxiv - Bioengineering 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were sourced from OriBiotech (Shanghai, China) and cultured in ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies, Vancouver, Canada). Mouse PBMCs were purified from mouse whole blood by Mouse PBMC Isolation Kit (Solarbio ...
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bioRxiv - Cancer Biology 2023Quote: An Easy Step Human CD8+ T Cell Isolation Kit was used to isolate CD8+ T cells from a donor PBMC sample via negative selection (Cat #, 17913, Stem Cell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2023Quote: ... malignant B cells were enriched from splenocytes of Eµ-TCL1 mice using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, Inc., Cologne, Germany). The CD5+ CD19+ content of purified cells was typically above 95% ...
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bioRxiv - Cancer Biology 2023Quote: HSCs from the BM of CD99 Gt/Gt and littermate WT mice were double sorted to >95% purity and seeded at a density of 150 cells/replicate into cytokine-supplemented methylcellulose medium (Methocult M3434; Stem Cell Technologies, Vancouver, Canada). Colonies number was scored after 10 days ...