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Citations for Stemcell Technologies :
1951 - 2000 of 4776 citations for Killer cell immunoglobulin like receptor 2DL2 KIR2DL2 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 0.24 mM all-trans retinoic acid (Stem Cell Technologies), 0.5 mM PD173074 (Tocris ...
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bioRxiv - Bioengineering 2024Quote: ... using DermaCult basal medium (Stem Cell Technologies 100-0501) with DermaCult keratinocyte expansion supplements (100–0502) ...
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bioRxiv - Bioengineering 2024Quote: ... 50 ng/mL Activin A (Stem cell technologies, 78001.1), 10 µM Y27632 ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were detached using Accutase (07922, STEMCELL Technologies) through incubation at 37°C for 5mins ...
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bioRxiv - Genetics 2024Quote: ... and cultured overnight in mTeSR media (Stem Cell Technologies). Differentiation was induced on day 0 by first washing with PBS ...
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bioRxiv - Bioengineering 2024Quote: ... single-cell dissociation was performed using Accutase (STEMCELL Technologies). H1 hESCs were supplemented with 10 µM Rock inhibitor for 24 hours post-dissociation ...
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bioRxiv - Bioengineering 2024Quote: ... density gradient in a Sep-Tube (Stem Cell Technologies). Monocytes were cultured for 5 days in RPMI media supplemented with 10 ng/mL of macrophage colony stimulating factor (MCSF ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1:1000 Heparin (Stem cell technologies Cat. # 07980). For GF medium ...
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bioRxiv - Developmental Biology 2024Quote: ... RFP-H9 cells were dissociated with ReLeSR (STEMCELL Technologies) as small clusters onto Matrigel-coated 6-well plates at low density ...
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bioRxiv - Developmental Biology 2024Quote: ... in mTESR Plus media (Stem Cell Technologies, #100-0276). Prior to EB formation ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged using Accutase™ (STEMCELL Technologies, 7922) and replated in MEF-CM or mTeSR™ containing 10 μM ROCK inhibitor Y-27632 (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... with 1.5 mL MyeloCult media (#H5100, Stem Cell Technologies) supplemented with 10-6M hydrocortisone (#H2270 ...
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bioRxiv - Cell Biology 2024Quote: Cells were cultured in mTeSRTM1 (STEMCELL Technologies, Cat. # 85850) supplemented with 1% penicillin/streptomycin (P/S ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were pelleted at 350x g for 2 min and transferred onto a 6-well transwell membrane (Stem Cell Technologies, #3450). Pellets were incubated with CHIR99021 (5 µM ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
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bioRxiv - Cell Biology 2021Quote: ... YTS cells were cultured in RPMI media supplemented with 10% fetal bovine serum and NK92 cells were cultured in Myelocult (Stemcell Technologies, catalogue H5100) supplemented with 200 U/ml of IL-2 (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Neuronal differentiation was performed using the protocol as described [59] with a modification that the cells were frozen at the neural progenitor stage at day 8 in CryoStor CS10 (Stem Cell Technologies #07931). Cells were thawed into LIA medium (ADF supplemented with 2mM Glutamax ...
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bioRxiv - Microbiology 2021Quote: ... we first isolated CD4+ T cells from 10M PBMC by negative selection using the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies, Cat #: 19052). This yielded a mean of 1.6 (range 1.3-2.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC). For intracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Cancer Biology 2020Quote: Clonogenic assay was realized with cells seeded into 35mm petri dish in semi-solid methylcellulose medium (Methocult™ M3231 for murin cells or Methocult™ H4230 for human cells, Stemcell Technologies). Cells were treated with the drugs for 72 hrs and centrifugated ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were needle triturated and resuspended in Mouse NSC Proliferation Media (1x Anti-Anti, 10ng/mL bFGF (#78003, Stem Cell Tech, Vancouver), 20ng/mL mEGF (#78016 ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells or CD56+CD3-NK cells were isolated from the PBMC population using the EasySep Human T Cell Isolation Kit or EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep™ Rat CD4+ T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells were extracted from blood drawn from healthy donors using density gradient separation (Lymphoprep, Stem Cell Technologies, Vancouver, BC, Canada) and cultured at 1 × 106 cells/ml in RPMI complete medium containing 1μg/ml Concanavalin A (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Erythroblasts were cultured in a serum-free mononuclear cell (MNC) medium containing the following cytokines diluted in Stem Span Serum Free Expansion Medium (Stem Cell Technologies, CA): 40□ng/mL insulin-like growth factor 1 (IGF1) ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were then seeded in triplicates at a concentration of 100,000 cells/well into 6-well culture plates with M3434 methylcellulose (Methocult, Stem Cell Technologies, Vancouver, Canada). For assessing the differentiation pattern of naïve bone marrow cells and MDPs in the presence of tumor-derived conditioned medium (TCM) ...
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
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bioRxiv - Genetics 2020Quote: ... Transduced cells were expanded to 10 cm plates and 10 vials (2M cells/vial) of each cell line was cryopreserved in CryoStor® (StemCell Technologies, 07930) for further use.
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bioRxiv - Cancer Biology 2020Quote: ... Cryopreserved MDS cells were thawed and cultured in StemSpan™ + cytokines (10ng/ml hIL3, 10ng/ml hSCF, 5ng/ml hIL6) overnight before plating cells in Methocult H4034 Optimum (Stem Cell Technologies) at a density of 1-2 × 104 cells per mL with DMSO ...
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bioRxiv - Immunology 2020Quote: ... Primary T cells were isolated from peripheral blood of healthy donors by negative selection using RosetteSept™ Human T Cell Enrichment Cocktail (STEMCELL Technologies, 15061) and activated with Dynabeads® Human T-Activator CD3/CD28 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632, Stem Cell Technologies #72308). A full protocol is available at the Allen Cell Explorer (www.allencell.org/methods-for-cells-in-the-lab SOP ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were isolated from the PBMC fraction using EasySep® naïve CD4+ T cell isolation kits and memory CD4+ T cell enrichment kits (StemCell Technologies, Meylan, France) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The cell mixture underwent centrifugation at 200g for 10min and then cultured using NeuroCult proliferation medium (Cat#05702, Stem Cell Technologies, Vancouver, Canada). The cells were maintained at standard conditions in a humidified 37 C° incubator containing 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... These cells were frozen as a master cell bank (passage 1) and working cell bank (passage 2) in CryoStor CS10 freezer medium (STEMCELL technologies, Vancouver, Canada), supplemented with Y-27632 (5 μM ...
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bioRxiv - Immunology 2022Quote: ... CD8+ and CD3+ T cells were isolated from PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit and EasySep™ Human T Cell Isolation Kit (both from STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from tumor-free wild type B6 mice using the mouse CD8 T cell isolation kit (STEMCELL Technologies cat #19853), labeled with Cell Trace Violet (Thermo Fisher cat #C34557 ...
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bioRxiv - Immunology 2022Quote: ... Splenic T cells were enriched using a magnetic-based commercial CD8 T cell negative selection kit (StemCell Technologies, BC, Canada; Supplemental Figure 3A) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... media was replaced every other day with neuronal differentiation media prepared based on the Stem Cell Technology protocol: 10 ml of the BrainPhys™ Neuronal Medium (#05790; Stem Cell Technology) supplemented with 200 µl of NeuroCultTM SM1 Neuronal Supplement (#05711 ...
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bioRxiv - Cell Biology 2023Quote: ... assay done by culturing 100-sorted cells LIN-CD34+ CD38- CD9+ or CD9- in to MethoCult™ H4434 with human cytokine (Stem Cell Technologies) and colony formation score at day 12-14 of incubation in 37°C.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were subcultured onto Matrigel at the first passage to avoid loss of the differentiated cells before tolerating the switch to MesenCult-ACF attachment substrate (Stem Cell Technologies, 05448). For passage two or above ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded at a density of 1.1×105 cells/well onto a 12 mm Transwell (ref 38023, STEMCELL TECHNOLOGIES, Saint Égrève, France) and grown in KSFM until cell confluency ...
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bioRxiv - Developmental Biology 2024Quote: ... quality control tests were routinely performed on hESC cultures, using a human stem cell pluripotency detection qPCR kit (Sciencecell, 0853) and hPSC genetic analysis kit (Stem Cell Technologies, 07550), according to the manufacturer’s protocols.
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bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: T cells were directly isolated from peripheral or cord blood using the RosetteSep Human T-cell Enrichment Cocktail according to the manufacturer’s protocol (Stem Cell Technologies cat#15061). T cells were cryopreserved in 90% fetal bovine serum+10% DMSO and stored in vapor phase liquid nitrogen following isolation ...
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bioRxiv - Developmental Biology 2022Quote: ... 1.25 million SOX2-mTurquoise2 / NANOG-mVenus H9 cells were resuspended in 1 ml of STEMdiff™ Neural Induction Medium (NIM, Stem Cell Technologies) with STEMdiff™ SMADi Neural Induction Supplement (SMADi ...
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bioRxiv - Immunology 2023Quote: ... Tcon and Treg cells were isolated from the spleen by pre-enrichment with EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, Cat# 19852), and FACS sorting ...
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bioRxiv - Bioengineering 2023Quote: ... human induced pluripotent stem cells (hiPSCs) were purchased from the Allen Institute for Cell Science and cultured in mTeSR1 media (Stemcell Technologies, Vancouver, Canada) supplemented with 10 μM Y-27632 (Stemcell Technologies ...
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bioRxiv - Genetics 2023Quote: ... we added the following procedures throughout this protocol: (1) we pretreated all plasticware that came into contact with cell aggregates with an anti-adhesion solution (Stem Cell Technology #07010) per manufacturer instructions and (2 ...