Labshake search
Citations for Stemcell Technologies :
2001 - 2050 of 2126 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Untouched PMNs were isolated from bone marrow in donor mice of the indicated genotype (age 6–8 wk) using EasySep mouse neutrophil enrichment kit (Stemcell Technologies Inc.). In a subset of experiment PMNs from C57/J animals were incubated with 1 mM Dimethyloxalylglycine (DMOG ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). Lineage-depleted bone marrow was then stained with an antibody cocktail comprised of a lineage cocktail (BioLegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). 2.5-3.0 × 106 lineage-depleted bone marrow was then washed in PBS and then resuspended in 135 μL Buffer T to which 15 μL of Dre mRNA (at 1 μg/μL ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... mTESR™ 1 medium was replaced with STEMdiff™ Hematopoietic Supplement A medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). On day 3 ...
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bioRxiv - Immunology 2020Quote: ... and CD8+ T cells were negatively isolated using the EasySep™ Mouse CD8+ T cell Isolation Kit (Stem Cell Technologies, Grenoble, France) or MojoSort™ Isolation Kits (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Immunology 2021Quote: ... CD4+ CD62L+ naïve T cells were isolated from 8-12 week-old mice using CD4 negative enrichment kits (Stemcell Technologies, Vancouver, Canada) and CD62L microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... from individual mice were pooled and pre-enriched for CD4 T cells using EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies). Live ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: ... Naïve T cells were isolated and purified from spleen and lymph nodes of C57BL/6J mice using EasySep™ Mouse CD4+ T Cell Isolation Kit (Stemcell Technologies).
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then sorted using magnetic beads conjugated with an anti-CD15 antibody (clone MC-480) (Kit easy step, Stem cell Technology).
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bioRxiv - Cancer Biology 2022Quote: ... pH 7.3] to remove red blood cells and CD8+ T cells were isolated via EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies,19853) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The cells were collected after an additional two days and pelleted for dead cell removal based on Annexin V using the Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, #17899).
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from lymph nodes of C57BL/6 Rag1-/+ OT-1-/+ mice and purified using the EasySep™ mouse CD8+ T cell isolation kit (STEMCELL Technologies) by negative selection ...
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bioRxiv - Immunology 2022Quote: ... Primary CD4+ T cells were isolated from spleens for NOD mice using Stemcell murine CD4+ T cell positive selection kit (Stemcell Technologies Inc).
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bioRxiv - Cancer Biology 2022Quote: ... Naïve CD8+ T cells were harvested from CD1 wildtype P25 mice and processed using the EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit (Stemcell Technologies, 19858). Following isolation ...
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bioRxiv - Cancer Biology 2024Quote: ... the spheres were chemically dissociated after seven days of plating, using the NeuroCult Chemical Dissociation Kit (mouse, #05707) from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
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bioRxiv - Immunology 2024Quote: The enzymatic activity of RALDH2 in BMDCs and MLN cells was assesses using the ALDEFLUOR Kit (#01700, STEMCELL Technologies, Vancouver, BC, Canada). DCs were pre-gated as CD11c+/MHC IIhigh cells and RALDH2 activity was detected by a flow cytometric analysis.
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bioRxiv - Immunology 2023Quote: ... T cells from the transgenic mouse splenocytes were enriched by magnetic isolation using the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies). T cells were centrifuged and resuspended in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... DE and LPM precursors were induced once seeded hiPSC-formed colonies by the Activin induction using the STEMdiff Definitive Endoderm Kit (StemCell Technologies, 05110) for 72 hours ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were first differentiated into hematopoietic progenitor cells using a combined strategy of incorporating primitive hematopoiesis via a WNT switch(77)and sorting and replating CD235a+ cells in Medium B from a commercially available kit (StemCell Technologies #05310). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... CD34+ progenitor cells were enriched using magnetic CD34 microbead kit (Miltenyl Biotec, San Jose, California) and expanded overnight in SFEMII (Stem Cell Technologies) supplemented with SCF (100 ng/mL) ...
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bioRxiv - Genomics 2023Quote: ... liver samples in cohort 2 were reduced of dead cell burden using the EasySep Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, 17899) to improve cell viability to >70% ...
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bioRxiv - Neuroscience 2023Quote: Human cerebral organoids were generated from WT and PCDH12-KO RUES1 hESCs and WT GM03651 and GM03652 iPSCs with the STEMDiff Cerebral Organoid Kit (StemCell Technologies, 08570) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... filtered through 40 µm cell strainers and T cells enriched by negative selection using an EasySep™ pan-T cell enrichment kit (Stemcell Technologies) as per manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: Mature astrocytes were created from astrocyte precursor cells using the STEMdiff Astrocyte Maturation media kit from STEMCELL Technologies (Cat No. 100-0016) following their guidelines for Matrigel-coated 6-well plates ...
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bioRxiv - Cell Biology 2023Quote: ... Human CD34+ cells were purified from fresh BM cells using MicroBead kit (Miltenyi Biotech 130-046-702) and subsequently cryopreserved in CryoStor® CS5 (STEMCELL Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
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bioRxiv - Immunology 2023Quote: CD63+ DCs or CD63- control DCs isolated from the LNs of Balb/c mice were sorted by FACS as described previously and cultured with naïve CD4+ T cells magnetically isolated from Fox3-GFP+ mice (C57Bl/6 background) using EasySep Mouse Naïve Cd4+ T cell isolation kit (Stemcell Technologies, Cat# 19765) at a ratio of 1:5 DC:T cell for 5 days in RPMI containing 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... apoptotic and dead cells were removed by immunomagnetic cell separation using the Annexin Dead Cell Removal Kit (StemCell Technologies, cat. no. 17899) and EasySep Magnet (StemCell Technologies ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from pooled splenic cell suspensions by magnetic negative selection using EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Self-organizing cerebral organoids were derived from induced pluripotent stem cells (iPSC) using the STEMdiff™ Cerebral Organoid Kit developed by STEMCELL Technologies™ (catalogue # 08570) ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells that passed through 70-uM cell strainer were purified with EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies Inc). For T-cell proliferation assays ...
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bioRxiv - Neuroscience 2024Quote: ... CD11b+ microglial cells were isolated by magnetic bead enrichment using the EasySep™ Mouse CD11b Positive Selection Kit (STEMCELL Technologies, Cat. 18970), according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... femurs and tibias of C57BL/6 (WT) and Jak/2E/B6 Stella Cre mice using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... mTSR+ media was replaced with fresh media A and the protocol was followed for the next 12-14 days as per kit instructions (Stem Cell Technologies). On day 12 and day 14 ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were washed in PBS and resuspended in PBS containing 2%FBS and 2 mM EDTA before negative selection using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, #19844A) following manufacturer instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... 12053231) and CD4+ and CD8+ T cells were isolated using a magnetic bead-based negative selection kit (easySEP, Stem Cell technology, 17951) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Brachial and Axillary) and CD4+ve T cells were isolated using EasySep™ Mouse CD4+ T Cell Isolation Kit (STEM CELL TECH). Protein lysate of snap-freeze tissue in liquid nitrogen was prepared by homogenizing 5mg tissue in 500μl ice-cold RIPA lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: Tumor-infiltrating lymphocytes were purified from dissociated MC38-derived tumors using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with DMEM (centrifuged at 300 g for 10 mins at 4°C) and further dissociated into single cells using the NeuroCult Chemical Dissociation Kit (STEMCELL Technologies; #05707) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Negative selection isolated naive B cells from mouse spleens using the EasySep™ Mouse B Cell Isolation Kit (cat. 19854, STEMCELL Technologies). The isolated B cells (1 x 105 ...
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bioRxiv - Cancer Biology 2024Quote: Mouse naïve CD4 T and B cells were isolated using mouse CD4 and B cell negative selection kits (Stemcell Technologies, Cambridge, MA). CD4+Foxp3GFP+ Tregs or CD4+Foxp3 GFP− naïve CD4 T cells were sorted from total CD4 T cells from LNs and spleens of WT or LTα−/− Foxp3-GFP mice using a FACS Aria II (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples for SLAM population analysis and cell sorting were first subjected to lineage-negative population enrichment using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: Bovine NPC direct differentiation protocol was modified based on the instruction of STEMdiff™ SMADi Neural Induction Kit (Stem Cell Technologies, 08581). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Human iPSCs and H9 human embryonic stem cells (hESCs) were maintained on a feeder-free system using Matrigel coated culture plates and TeSR-E8 media Kits (STEMCELL Technology Inc.).27 All culture media and reagents were purchased from Invitrogen (Carlsbad ...