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Citations for Stemcell Technologies :
1951 - 2000 of 2126 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: Whole blood samples of 5-10 mL were collected in EDTA Vacutainer test tubes and processed for the isolation of erythroid progenitor cells (EPCs) using the Erythroid Progenitor Kit (StemCell Technologies). EPCs were reprogrammed using Sendai particles (CytoTune-iPS 2.0 Reprogramming kit ...
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bioRxiv - Immunology 2023Quote: ... the spleen of the mouse with the highest antibody titers was dissociated and fused with Sp2/0-Ag14 myeloma cells (ATCC) using the ClonaCell-HY Hybridoma Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The genetic integrity of the engineered hiPSC clones was evaluated by a qPCR-based hiPSC genetic analysis kit (Stem Cell Technologies).
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bioRxiv - Immunology 2023Quote: ... splenocytes were fused with myeloma cells P3X63Ag8 (ATCC, #TIB-9) using ClonaCell-HY Hybridoma Kit according to manufacturer’s instructions (StemCell Technologies, #03800). Culture supernatants were screened using ELISA (see below ...
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bioRxiv - Immunology 2023Quote: ... Naive B cells were isolated by negative magnetic activated cell sorting using the EasySepTM Mouse B cell Isolation Kit (StemCell Technologies). 30 million B cells were injected i.p ...
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bioRxiv - Cancer Biology 2023Quote: CD8 T cells were isolated from the lymph node of naïve mice with unactivated Confetti cassette using EasySep™ Mouse CD8+ T Cell Isolation Kit (StemCell Technologies) and macrophages were sorted from the tumors via FACS sorting ...
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bioRxiv - Immunology 2023Quote: Small resting B cells were isolated from spleen with a discontinuous Percoll gradient and subsequent purification using EasySep Mouse B cell Isolation Kit (StemCell Technologies). B cells were stimulated with 3mM CpG (Sigma) ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from peripheral lymph nodes (inguinal, axillary, brachial and superficial cervical) by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies). CD4+ T cells were stimulated in 96 well flat-bottomed plates coated with 20mg/ml αCD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... ETRAMP-specific CD4+ T cells were enriched using APC- and PE-conjugated tetramers and EasySep APC and PE Positive Selection Kits (STEMCELL Technologies). Cells were then stained with fluorescent dye-conjugated antibodies (see Table I ...
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bioRxiv - Cancer Biology 2024Quote: The ALDH activity of oral cancer cells co-cultured with CAF was measured using the ALDEFLUOR assay kit (Cat. #01700, StemCell Technology), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... OT-I+/- or total CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection using the EasySepTM Mouse CD8+ T cell isolation kit (#19753, StemCell Technologies). For in vitro experiments ...
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bioRxiv - Immunology 2024Quote: ... The resulting hybridoma cells were selected on semi-solid methylcellulose and grown in 96-well plates using the ClonaCell-HY Hybridoma Cloning Kit (Stemcell Technologies). Hybridomas secreting SH-01-reactive IgG were identified by several rounds of ELISA screening ...
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bioRxiv - Neuroscience 2024Quote: ... mTESR plus medium was replaced with STEMdiff™ Hematopoietic Supplement A medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). On day 3 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then harvested and stained using the Annexin V Apoptosis Detection Kit with 7-AAD (STEMCELL Technologies, Vancouver, BC, Canada), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were plated at density of 65,000 cells per well in Neural Induction Media (STEMdiff SMADi Neural Induction Kit STEMCell Technologies, 08581) in a 96-well v-bottom plate to form neural aggregates ...
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bioRxiv - Neuroscience 2024Quote: hiPSC derived neural progenitor cells (NPCs) were generated using the embryoid body protocol and STEMdiff SMADi Neural Induction Kit (STEMCELL Technologies). From these NPCs ...
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bioRxiv - Developmental Biology 2024Quote: ... EB-HSPCs, and primary mPBSCs from a healthy donor (female, 30 years) were differentiated using the StemDiff T Cell Kit (STEMCELL Technologies) with minor modifications to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells (OT-I cells) were isolated by negative selection with the EasySepTM Mouse CD8 T cell isolation kit (Catalog #19853, Stemcell Technologies) following the manufacturers protocol ...
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bioRxiv - Immunology 2024Quote: ... Cells were filtered through a 70 µm nylon strainer and further purified with the EasySepTM mouse neutrophil enrichment kit (StemCell Technologies), following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... femur and tibia were flushed and neutrophils (PMN) enrichment was performed with the EasySep mouse neutrophil enrichment kit (Stem Cell Technologies). PMN ROS production upon stimulation with PMA (100 nM ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T were isolated from the spleen and LN of OT-II TCR Tg GFP+ mice using the Easysep Mouse CD4+ T cell isolation kit (Stemcell Technologies) or MojoSort Mouse CD4 Naïve T cell isolation kit (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... with minor modifications.18 CD34+ cells were isolated directly from PBMCs or from enriched HSPCs (RosetteSep Hematopoietic Progenitor Enrichment Cocktail Kit, Stemcell Technologies) via immunomagnetic isolation (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2024Quote: ... CD8+/CD4+T cells (for OT-I/OT-II mice) were isolated from this suspension using the EasySep Mouse CD8+/CD4+ T Cell Isolation kit (STEMCELL Technologies; Cat#19852 for CD4+T ...
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bioRxiv - Cancer Biology 2024Quote: CD8+ T cells were isolated from the mouse spleen and injected with mEERL cells with scramble or Marchf8 sgRNA using the CD8+ T-cell Isolation Kit (STEMCELL Technologies). The cells were labeled with 3 µM CFSE (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: RNA extraction and quantitative PCR: Tumor infiltrating myeloid cells were isolated from tumors either using a CD11b isolation kit (catalog # 18970, StemCell Technologies) or by cell sorting ...
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bioRxiv - Cancer Biology 2024Quote: CD8+ T cells were isolated from the spleens of C57BL/6J or mice with magnetic bead-based CD8 T cell isolation kit (Cat # 19853, StemCell Technologies). T cells from naïve mice were stained with 5µM Cell Trace Violet (Cat# C34557 ...
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bioRxiv - Cancer Biology 2024Quote: Mouse spleens were mechanically disrupted over a 100-µm filter and subjected to magnetic bead negative enrichment for CD8+ T cells using a CD8+ T-cell Isolation Kit (STEMCELL Technologies). Isolated CD8+ T cells were cultured in RPMI (Gibco ...
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bioRxiv - Cell Biology 2024Quote: The differentiation of iPSCs into the three germ layers was performed using the STEMdiff™ Trilineage Differentiation Kit (STEMCELL Technologies, #05230).
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bioRxiv - Bioengineering 2024Quote: ... and endoderm was performed on 48-well plates (500 µl/well feed volume) using the STEMdiff Trilineage Differentiation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Neurospheres were dissociated into single cells and passaged every seven days using a chemical dissociation kit (Catalog# 05707, STEMCELL technologies, U.S.A.). Fresh neurobasal medium (containing all the supplements ...
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bioRxiv - Developmental Biology 2024Quote: H1 parental and modified hES cells were differentiated into PP cells using the STEMdiff™ Pancreatic Progenitor Kit (STEMCELL Technologies, 05120) according to the manufacturer’s instructions or using an adaptation of published procedures 8,10,51 with changes as described (Table S5) ...
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bioRxiv - Immunology 2021Quote: OVA (SIINFEKL)-specific CD8+ T cells were isolated from the spleen and lymph nodes of healthy 6-8-week old OT-I mice using EasySep™ Mouse CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: Murine T-cells were isolated from spleens of healthy 6-8-week old C57BL/6 mice using EasySep™ Mouse CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: Feeder-free H1 hESCs (WiCell) were cultured on Matrigel in TeSR™-E8™ kit for hESC/hiPSC maintenance (StemCell Tech; #05990). hESCs were used to generate COs using media included in the STEMdiff Cerebral Organoid Kit (StemCell Tech ...
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bioRxiv - Immunology 2021Quote: NK cells were purified from uninfected spleens of WT and CypD-/- mice using the EasySep Mouse NK Cell Isolation Kit (Stem Cell Technologies) according to the supplier’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: ... the sample was sorted using an APC-conjugated antibody to NOTCH1 (eBiosciences #17-5765-80) followed by APC magnetic selection using the EasySep kit (Stem Cell Tech) as per instructions and plated in PneumaCult medium with a SMAD inhibitor ...
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bioRxiv - Immunology 2021Quote: ... C57BL/6.OT-I.Ubiquitin-GFP CD8+ T cells were enriched from single-cell suspensions of lymph nodes and spleens using mouse CD8-negative selection kits (Stem Cell Technologies). Enriched CD8 T cells (5×106 ...
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bioRxiv - Cancer Biology 2020Quote: ... we thawed frozen viable breast cancer pleural effusions or ascites from patients and depleted dead cells with EasySep™ Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies) and CD45+ cells with EasySep™ Human CD45 Depletion Kit II (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... iPSCs were differentiated into hematopoietic progenitor cells (HPCs) over the course of 11 days using the STEMdiff Hematopoietic kit (STEMCELL technologies, 05310). Non-adherent cells were then collected and analyzed ...
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bioRxiv - Microbiology 2021Quote: Cortical organoid cultures were differentiated from the healthy iPSC cell line according to the specification of the cerebral organoid kit from StemCell Technologies (#08570) that relies on an established protocol (54) ...
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bioRxiv - Immunology 2021Quote: ... Neutrophils were isolated from the remaining blood pellet via negative selection using the EasySep Direct Neutrophil Isolation Kit (STEMCELL Technologies, Cat# 19666) following manufacturer’s directions ...
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bioRxiv - Developmental Biology 2022Quote: ... All tissues were subject to removal of red blood cells (RBCs) using immunomagnetic negative selection with the EasySep RBC Depletion kit (STEMCELL Technologies, 18170). 12 w samples were further subjected to EasySep™ Human EpCAM Positive Selection Kit II (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... CAR T cells were positively selected for truncated CD19 using the EasySep™ CD19 Positive Enrichment Kit I or II (StemCell Technologies) (for PSCA-CAR T cells ...
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bioRxiv - Cell Biology 2022Quote: ... iPSC-CM was generated as previously described (ref) using GiWi method (17) or STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit (STEMCELL Technologies, 05010), then further purified by lactate selection (18) ...
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bioRxiv - Cancer Biology 2022Quote: ... were harvested from 6-12 weeks old in-house bred BL/6 mice (WT or transgenic CD2 Cre/CD4 Cre mice) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies). HSPCs were transduced with retrovirus for expression of the desired oncogenes ...
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bioRxiv - Developmental Biology 2021Quote: hESCs were single-cell seeded onto 6-well Matrigel-coated plates at 2*106 cells/well for ectoderm and endoderm conditions and 5*105 cells/well for mesoderm condition and treated with the appropriate differentiation medium using the STEMdiff Trilineage Differentiation Kit (StemCell Technologies #05230) according to manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: Leukopak-derived peripheral blood mononuclear cells (PBMCs) purchased from LifeSouth Community Blood Centers were debulked with RosetteSep CD8+ enrichment kits (Stem Cell Technologies), and then separated into an enriched fraction of PBMC using a Ficoll gradient ...
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse CD8+ T cells were isolated from the mouse spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19853A) according to manufacturer instructions ...