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Citations for Stemcell Technologies :
151 - 200 of 209 citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were plated immediately into pre-warmed antibiotic-free HSPC media (StemSpan SFEM II; Stemcell Technologies; cat. no., 09605_C) supplemented with SCF (100 ng/ml ...
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bioRxiv - Bioengineering 2021Quote: ... the cuvettes were allowed to rest for 15 minutes before the T cells were gently moved to antibiotic-free medium containing 1µg/mL DNase (StemCell Tech) at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
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bioRxiv - Neuroscience 2022Quote: iPSCs were cultured in feeder-free conditions on Matrigel-coated (0.08 mg/well) 6-well tissue culture plates in mTesR1 media (StemCell Technologies) at 37 °C and 5 % CO2 ...
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bioRxiv - Genomics 2023Quote: Unedited and WDR90 knock-out iPSC clones were transitioned into feeder-free condition in mTeSR™ Plus medium (STEMCELL Technologies) in a matrigel-coated 6 well plate at a density of 5x105 cells/well with 5 µM ROCK Inhibitor ...
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bioRxiv - Immunology 2019Quote: Cells were cultured at 37°C under 5% CO2 in 100 μl of StemSpan serum-free expansion medium (STEMCELL technologies 9650) supplemented with cytokine cocktails aimed at proliferation (50 ng/ml murine recombinant thrombopoietin (TPO ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... between 50-250*103 cells per 200µl of the following culture media: Human LT-HSCs (LIN−/CD38lo/CD34+/CD45RA−/CD90+) in StemSpan Serum-free expansion medium (SFEM; Stemcell Technologies) supplemented with 100ng/ml human SCF ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were cut into 1-2 mm pieces and subsequently incubated in serum-free medium containing collagenase/hyaluronidase (Stem Cell Technologies) at 37 ºC ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were allowed to recover in antibiotic-free medium containing 1 µg/mL DNase I solution (STEMCELL Technologies, Cambridge, MA) at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... CD34+ cells from MF patients were isolated as described above and cultured in StemSpan serum free medium (SFEM; cat# 09605, StemCell Technologies) with stem cell factor (SCF ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary patient material was aliquoted into 24- or 48-well plates in 200μl StemSpan™ Serum-Free Expansion Medium (SFEM) (STEMCELL Technologies) supplemented with 20% FCS and the following cytokines ...
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bioRxiv - Cancer Biology 2022Quote: CD34-positive and Lin-negative cells from PBMC were single-cell sorted into 96-well plates containing 100 μL of free medium consisting of StemSpam SFEMII (Stemcell Technologies) with human cytokines SCF ...
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bioRxiv - Genetics 2022Quote: ... HSPCs were transferred into erythroid differentiation medium (EDM) consisting of StemSpan serum-free expansion medium II(SFEM II; Stem Cell Technologies) with 1% penicillin/streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... The CHOP-WT10 cell line was maintained as an iPSC culture35 and subsequently transitioned to feeder-free cultures and maintained with mTeSR1 (StemCell Technologies) on hESC qualified matrigel (Corning) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured in feeder-free medium and grown on Matrigel-coated plates in cell medium containing 1:1 mTesR1: Essential 8 (StemCell Technologies, 85850 and Thermofisher A1517001 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 104 sorted cells were plated in triplicate in serum-free media containing StemSpan™ Megakaryocyte Expansion Supplement (STEMCELL Technologies) for 10-12 days ...
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bioRxiv - Genomics 2023Quote: ... The iPSCs were transitioned into feeder-free conditions inhouse and maintained as feeder-free cultures in mTeSR Plus basal medium supplemented with mTeSR Plus 5X supplement (StemCell Technologies). The cultures were grown in multiwell plates coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then passaged using GCDR and replated on 10-cm dishes coated with Animal Component Free (ACF) Attachment Substrate (StemCell Technologies); all subsequent passages were grown using the same medium and culture substrate ...
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bioRxiv - Cell Biology 2022Quote: ... were obtained from the CCHMC Pluripotent Stem Cell Facility (PSCF) (44) and cultured in feeder-free condition with mTeSR1 (85850, StemCell Technologies) and on a hESC-qualified Matrigel (354277 ...
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bioRxiv - Genomics 2023Quote: ... hESC was maintained and expanded in feeder-free culture conditions with mTeSR basal medium (STEMCELL Technologies Inc., Vancouver, Canada, cat # 85850) on 6-well plates culture-treated coated with Corning Matrigel hESC-qualified Matrix ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed and resuspended in a fresh pre-warmed complete culture medium and cultured in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ cells were seeded at the concentration of 5*105 CD34+ cells/ml in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 106 lineage negative cells/ml were transduced with 1 x 108 TU/ml of empty vector or Vegfc lentivirus in serum-free Stem-Span medium (StemCell Technologies) supplemented with 10 ng/ml IL-3 ...
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bioRxiv - Cell Biology 2024Quote: ... Semi-solid MC3434 cell mixtures were plated at 1 mL mixture/well in a 6-well meniscus-free dish (SmartDishTM, STEMCELL Technology) using a 3cc syringe and a blunt-end 16-gauge needle (STEMCELL Technology) ...
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bioRxiv - Cell Biology 2023Quote: For cell growth assay 100 LIN-CD34+CD38- CD9+ or CD9- cells were sorted and seeded in 96 well plate and culture in StemSpan Serum –Free Expansion Medium (SFEM, StemCell Technologies) supplemented with 100u/ml penicillin (Hyclone ...
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bioRxiv - Neuroscience 2024Quote: ... in a feeder free fashion and passaged every 4∼5 days at a 1:4∼1:6 ratio using ReLeSR (Stemcell technologies) as described previously 88,95 ...
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bioRxiv - Developmental Biology 2024Quote: ... SSI and SSII media are parts of a 2-step suspension culture system with a base medium consisting of StemSpan Serum-Free Expansion Medium II (StemCell technologies), 2 mM of L-glutamine ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were maintained in feeder free conditions, batch fed daily with Essential 8(Chen et al., 2011) or mTeSR⅄1 (STEMCELL Technologies, 85850) cell culture media ...
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bioRxiv - Neuroscience 2019Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cancer Biology 2020Quote: Feeder-free H1 hESCs (WiCell) were cultured on Matrigel in TeSR™-E8™ kit for hESC/hiPSC maintenance (StemCell Tech; #05990). hESCs were used to generate COs using media included in the STEMdiff Cerebral Organoid Kit (StemCell Tech ...
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice a week using an enzyme-free dissociation reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Genomics 2021Quote: ... were sorted using a BD FACSAria™ Fusion Cell Sorter into ice-cold Serum-Free Expansion Medium (SFEM) supplemented with 100 ng/ml SCF and Flt3 (Stem Cell Technologies), 20 ng/ml IL-3 ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed cells were cultured at 37°C and 5%O2 in serum-free HSC media comprised of StemSpan II medium (Stem Cell Technologies) supplemented with CC100 cytokine cocktail (Stem Cell Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies). For autophagy induction cells were treated with 200 µM Rapamycin (ChemCruz ...
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bioRxiv - Neuroscience 2020Quote: ... gene were cultured according to Stem Cell Technologies protocols that can be found in (https://www.stemcell.com/maintenance-of-human-pluripotent-stem-cells-in-mtesr1.html) on feeder free in hESCs medium on Matrigel (StemCell Technologies, Cat. #354277) in mTeSR (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... NK cells were allowed to recover at a density of 1.5 × 106 cells/mL in antibiotic-free medium containing 1 ug/mL DNase I solution (STEMCELL Technologies, Cambridge, MA), and were then cultured in complete NK cell medium at a density of 6 × 105 cells/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... HSPCs were isolated from Tie2-Cre control (wild-type) or Tie2FF1 (JAK2V617F) and cultured overnight in StemSpan serum-free expansion medium (SFEM) (Stem Cell Technologies) containing 100 ng/mL recombinant mouse SCF ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was refreshed every 2-3 days and hiPSCs were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Developmental Biology 2021Quote: Culture of isolated human CB-derived CD34+ cells was performed in HSPC expansion media comprised of Stem Span serum-free media (StemCell Technologies #09855) supplemented with Glutamax (Invitrogen #35050061) ...
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bioRxiv - Developmental Biology 2022Quote: KOLF2 iPS cells were grown under feeder-free conditions on Matrigel-coated plates in TeSR™-E8™ (Stem Cell Technologies, #05990).
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bioRxiv - Neuroscience 2022Quote: ... the iPSCs were seeded in 6-well plates coated in phenol-red free Matrigel with hematopoietic differentiation media (StemCell Technologies, cat.no. 05310). The media was changed following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... were cultured according to Stem Cell Technologies protocols that can be found in (https://www.stemcell.com/maintenance-of-human-pluripotent-stem-cells-in-mtesr1.html) on feeder free in hESCs medium on Matrigel (StemCell Technologies, Cat. #354277) in mTeSR (Stem Cell Technologies ...
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bioRxiv - Genomics 2023Quote: ... directly into ice-cold complete medium (Stemspan serum-free expansion medium (SFEM) supplemented with 100 ng/ml SCF and Flt3 (Stem Cell Technologies) and 20 ng/ml IL-3 ...
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bioRxiv - Bioengineering 2023Quote: ... Medium was refreshed every 2–3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... H9 human embryonic stem cells (Agreement No. 19-W0538; WiCell, USA) were maintained as bulk culture in feeder-free conditions on vitronectin (StemCell Technologies, Canada)-coated dish in TeSR-E8 basal medium plus supplement (StemCell Technologies ...
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bioRxiv - Bioengineering 2023Quote: The human embryonic stem cell (hESC) line ‘Man13’ were cultured as colonies under feeder-free conditions in TeSR-E8 culture medium (StemCell Tech 05990), on 6-well tissue culture plates pre-coated with VTN (GIBCO 14700)1 ...
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bioRxiv - Cell Biology 2023Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Molecular Biology 2024Quote: ... Lin- cells were transduced overnight at a viral multiplicity of infection (MOI) of 0.3-50 (Table S6) in StemSpan Serum Free Expansion Medium (SFEM) culture medium (StemCell Technologies, Vancouver, Canada) supplemented with 50 ng/mL recombinant murine stem cell factor (SCF) ...
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bioRxiv - Genetics 2024Quote: ... CD34+ HSPCs were cultured at 1×105–5×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies, Vancouver, Canada) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM ...
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bioRxiv - Neuroscience 2021Quote: ... The transfected LCLs were then plated into a matrigel-coated six-well plate and maintained in TeSr-E7 xeno-free containing media (Stem Cell Tech Inc.). Twelve days after transfection ...