Labshake search
Citations for Stemcell Technologies :
101 - 150 of 209 citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... hiPSCs were then incubated in imaging media of phenol red-free mTeSR1 media (Stem Cell Technologies) with 1% penicillin streptomycin (Thermo Fisher ...
-
bioRxiv - Neuroscience 2022Quote: ... The iPSC lines were cultured on feeder-free Matrigel-coated plates in mTeSR media (StemCell Technologies) before differentiation into forebrain cerebral organoids.
-
bioRxiv - Genomics 2023Quote: ... iPSCs were maintained in feeder-free conditions in mTESR1 (85850, Stem Cell Technology, Vancouver, BC, Canada) with 1% Penicillin/Streptomycin (30-002-Cl ...
-
bioRxiv - Bioengineering 2023Quote: ... the cells were dissociated using Animal Component-Free (ACF) enzymatic dissociation solution (Stemcell Technologies, Cologne, Germany). Then ...
-
bioRxiv - Developmental Biology 2024Quote: ... hESCs were cultured under feeder-free conditions in mTeSR Plus media (Stem Cell Technologies, 100-0276) on plates coated with Matrigel (Corning ...
-
bioRxiv - Neuroscience 2024Quote: ... GBM cells were grown in serum-free Neurocult NS-A Basal medium (Stem Cell Technologies #05750) that is supplemented with 2mM L-Glutamine (Wisent #609—65-EL) ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were cultured in StemSpan™ Serum-Free Expansion Medium version II (SFEM II; STEMCELL Technologies) containing 50 ng/mL each of recombinant human stem cell factor (SCF ...
-
bioRxiv - Cancer Biology 2023Quote: ... 250 cells were plated in triplicate in cytokine-free methylcellulose media (Methocult GM M3234, StemCell Technologies) supplemented with 20 ng/mL IL-3 and 20 ng/mL SCF (R&D Systems) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were sorted directly into 200 μl Stem Span Serum-Free Expansion Medium (SFEM, StemCell Technologies), supplemented with 100 units/ml penicillin ...
-
bioRxiv - Pathology 2021Quote: ... washed 5x with water and prefilled with 100 uL per well of Neural Coating Medium (NCM) comprising of Brainphys medium (STEMCELL Technologies) with 1X B27+Vit.A ...
-
bioRxiv - Genetics 2023Quote: ... washed five times with water and prefilled with 100 µl per well of neural coating medium comprising Brainphys medium (STEMCELL Technologies) with 1× B27 plus vitamin A ...
-
bioRxiv - Bioengineering 2022Quote: ... were either coated with 70 µL of 2% agarose in water or PBS or treated for 20 min with 100 µL of anti-adherence rinsing solution (StemCell Technologies 07010). The wells were washed with PBS ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells for feeder free systems were passaged with a non-enzymatic disassociation solution ReLeSR (Stem Cell Technologies) according to manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2020Quote: Flow sorted cells were seeded in serum-free methylcellulose-based medium with cytokines (MethoCult SFH4436, Stemcell technologies). 5 μM imiquimod or DMSO were added to the methocult at the time of seeding in a separate location within the media prior to vortexing ...
-
bioRxiv - Bioengineering 2021Quote: ... 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF, STEMCELL Technologies, 78139), 0.2% Chemically Defined Lipid Concentrate (Thermofisher ...
-
bioRxiv - Bioengineering 2021Quote: ... 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF, STEMCELL Technologies, 78139), 0.2% Chemically Defined Lipid Concentrate (Thermofisher ...
-
bioRxiv - Neuroscience 2021Quote: ... under a feeder-free condition and were passaged once per week with ReLeSR medium (STEM CELL Technologies). All hPSC studies were approved by the Embryonic Stem Cell Research Oversight committee at Rutgers University.
-
bioRxiv - Neuroscience 2021Quote: ... UK) at passage 32 were maintained under feeder-free conditions on vitronectin (STEMCELL Technologies, British Columbia, Canada)-coated 6-well plates in E8 medium (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: ... G797 cells were maintained in serum-free NeuroCult™ NS-A Basal Medium (STEMCELL Technologies Canada Inc) supplemented with N2 ...
-
bioRxiv - Developmental Biology 2021Quote: ... The hPSC lines were cultured in serum-free defined conditioned media mTeSR or mTeSR plus (STEMCELL Technologies) on hES-certified matrigel (Corning cat#354277) ...
-
bioRxiv - Systems Biology 2021Quote: H1 male hESC cell line was maintained under feeder-free conditions in mTeSR1 medium (Stem Cell Technologies) supplemented with 1% antibiotic-antimycotic (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2021Quote: ... The isolated monocytes were counted and resuspended in growth medium (Stemcell Technologies, DC differentiation Medium, serum-free) supplemented with GM-CSF (1000 U/mL ...
-
bioRxiv - Immunology 2023Quote: ... and stem cells were passaged weekly using enzyme free dissociation reagent ReLeSR™ (Stem Cell Tech #05872). Cultures were maintained in an undifferentiated state ...
-
bioRxiv - Genetics 2023Quote: ... were transitioned from MEF-dependent culture to feeder-free culture using mTeSR® medium (Stem Cell Technologies) and 6-well plates coated with hESC-qualified Matrigel® (Corning ...
-
bioRxiv - Cancer Biology 2019Quote: ... AMKL patient cells maintained in immunodeficient mice were cultured in StemSpan™ Serum-Free Expansion Medium (STEMCELL Technologies) supplemented with 100 U/ml penicillin ...
-
bioRxiv - Biochemistry 2020Quote: ... H9 cells were cultivated in feeder-free conditions with mTESR basal media supplemented with mTESR supplement (Stemcell Technologies) and on 15cm Matrigel (BD)-coated tissue culture plates ...
-
bioRxiv - Neuroscience 2020Quote: Feeder-free E14Tg2a (Baygenomics) mouse embryonic stem cells (mES) were passaged on gelatin-coated (0.1% gelatin, StemCell Technologies) cell culture treated plastic dishes using established media and cell culture techniques (Sadegh and Macklis ...
-
bioRxiv - Cell Biology 2022Quote: ... medium was removed and 5,000 BM CD34+ cells were added in serum-free expansion medium (SFEM, STEMCELL Technologies) supplemented with or without stem cell factor ...
-
bioRxiv - Neuroscience 2022Quote: ... H9 hESC were maintained in feeder-free conditions without antibiotics in mTeSR™ Plus (Stem Cell Technologies 05825) on hESC qualified Matrigel (Corning ...
-
bioRxiv - Neuroscience 2021Quote: ... The iPSCs were maintained under feeder-free conditions over Matrigel-coated plates in mTeSR Plus media (Stemcell Technologies). The media was changed daily and the iPSC cultures were split into 1:6-1:10 every 5 days using ReLeSR (Stemcell Technologies).
-
bioRxiv - Cancer Biology 2020Quote: ... The cell pellets were then washed twice in Ca2+ and Mg2+ free PBS (Stem Cell Technologies, Vancouver, Canada). The cell pellets were digested with a standard volume of suprapur nitric acid and hydrogen peroxide (Merck Millipore ...
-
bioRxiv - Bioengineering 2019Quote: ... cultures were initiated at a density of 500,000 cells/mL with serum-free StemSpan SFEM (Stem Cell Technologies) supplemented with Hydrocortisone (1µM) ...
-
bioRxiv - Biophysics 2020Quote: ... Patient-derived GBM cells were plated in NeuroCult NS-A serum-free medium (StemCell Technologies, Vancouver, BC, Canada) supplemented with 20 ng/ml of epidermal- and 10 ng/ml of fibroblast-growth factor (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2021Quote: ... colonies were picked manually and expanded on feeder-free Matrigel-coated dishes and frozen with CryoStore (STEMCELL Technologies). Mycoplasma contamination was routinely checked.
-
bioRxiv - Cell Biology 2022Quote: ... Cells were plated onto 25 mm MatTek dishes and imaged in phenol-red free mTeSR1 (Stemcell Technologies, 85850). Cells were kept under 5% CO2 and 37 degrees C ...
-
bioRxiv - Neuroscience 2023Quote: ... The MSE2104 iPSC line was maintained by culturing in feeder-free condition incomplete mTeSR E8 medium (StemCell Technologies) in a humidified incubator (5% CO2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... CD34+ HSPCs were cultured at 1×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM ...
-
bioRxiv - Molecular Biology 2023Quote: ... iPSC colonies were picked 25–30 days post-transduction and maintained in feeder-free E8 media (StemCell Technologies) on a Matrigel (Corning) ...
-
PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... the HSCs were first cultured in StemSpan™ Serum-Free Expansion Medium (SFEM) II (09655, STEMCELL Technologies, Canada) supplemented with 2 μg/mL puromycin (GC16384 ...
-
bioRxiv - Cell Biology 2020Quote: ... Akt-transduced BMECs were selected for seven days in serum- and cytokine-free StemSpan SFEM (StemCell Technologies, Inc. 09650) media ...
-
bioRxiv - Biochemistry 2019Quote: ... Embryoid bodies (EBs) were generated from feeder-free iPSCs by incubating colonies with Collagenase Type IV (7909, StemCell Technologies) for 10 min ...
-
bioRxiv - Biophysics 2019Quote: ... Cells were washed and resuspended in phenol red-free Neurobasal Medium (Lifetech) supplemented with L-glutamine (Stem Cell Technologies) and SM1 (NeuroCult ...
-
bioRxiv - Cancer Biology 2019Quote: Neurosphere (sphere) cultures were established in serum-free media as described (9) (NeuroCult™ Media, Stem Cell Technologies, #05700) supplemented with human PDGF-AA (20 ng/mL ...
-
bioRxiv - Neuroscience 2020Quote: ... LIN28 and shRNA against p53 [28] in feeder- and serum-free conditions using TeSR-E7 medium (Stem Cell Technologies). Subsequently ...
-
bioRxiv - Cell Biology 2022Quote: ... mESCs were cultured in feeder free conditions with 5% CO2 at 37°C on 0.1% gelatin (StemCell Technologies, 7903) coated plates in 2i medium supplemented with 2% mES certified FBS (Gibco 16141061) ...
-
Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... The cells were cultured in feeder-free conditions in 10 cm culture dishes in TeSR-E8 (Stem Cell Tech) medium on SyntheMAX (2µg/cm2 ...
-
bioRxiv - Pathology 2019Quote: ... All iPSC lines were cultured on LDEV-free hESC-qualified Geltrex-coated tissue culture dishes in mTeSR1 (Stemcell Technologies) medium supplemented with 1% penicillin-streptomycin ...
-
bioRxiv - Cancer Biology 2022Quote: ... We grew cells in a 5% CO2 incubator in a serum-free NeuroCult NS-A basal medium (STEMCELL Technologies) supplemented with NeuroCult™ Proliferation Supplement (STEMCELL Technologies) ...
-
bioRxiv - Cell Biology 2023Quote: ... 5% CO2) at a cell concentration of 5 × 105/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies) supplemented with 100 ng/mL of human recombinant SCF ...
-
bioRxiv - Neuroscience 2023Quote: ... and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR; Stem Cell Technologies, 05872). Cells were checked for mycoplasma contamination every two weeks ...