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Citations for Stemcell Technologies :
1751 - 1768 of 1768 citations for Human Immunodeficiency Virus Nef Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... the media was changed to pre-warmed 37°C Medium DE-1 (STEMdiff™ Endoderm Basal Medium containing Supplement MR and Supplement CJ, Stem Cell Technologies). One well of seeded cells was left untreated as negative control (data not shown) ...
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bioRxiv - Genetics 2023Quote: ... we added the following procedures throughout this protocol: (1) we pretreated all plasticware that came into contact with cell aggregates with an anti-adhesion solution (Stem Cell Technology #07010) per manufacturer instructions and (2 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Neuroscience 2023Quote: ... 3 million iPSCs per cell line were plated into 1 well of an AggreWell™800 24-well plate (Stem Cell Technologies, 34811) with 2 mL STEMdiff™ Neural Induction Medium + SMADi + 10 µM Y-27632 ...
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bioRxiv - Biophysics 2024Quote: ... hiPSCs from N=1 donor were differentiated to definitive endoderm by using the STEMdiff™ Definitive Endoderm Kit (STEMCELL Tech., Vancouver, BC, Canada). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... and then re-seeded in supplemented mTeSR1 medium with 1% penicillin–streptomycin and a 10 μM concentration of the ROCK inhibitor Y-27632 (Stem Cell Technologies, 72308). Further details for cell culture reagents and consumables can be found in the Resource Table and standard protocols can be found at www.allencell.org.
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bioRxiv - Bioengineering 2020Quote: MSCs at passages 1 or 3 were seeded in wells of a 12-well plate containing 3 ml Mesencult (Stem Cell Technologies, Vancouver, B.C.) each at 8.0×104 ...
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bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...
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bioRxiv - Immunology 2022Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×106 T cells/ml in serum-free StemCell Immunocult™-XF media (Cat#10981, StemCell Technologies, Vancouver, Canada) with 20U/ml clinical grade human IL-2 (Novartis) ...
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bioRxiv - Neuroscience 2023Quote: ... Daily media changes of E7™ Reprogramming Media followed until the emergence of iPSC colonies between day 21-28 when iPSC colonies were picked and transferred to Geltrex™ coated 12-well plates containing 1 mL TeSR™-E8™ (STEMCELL Technologies) supplemented with 10 μM ROCK Inhibitor (Y-27632 ...
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bioRxiv - Bioengineering 2024Quote: ... Isolated T cells were seeded in the CD3/CD28 precoated well plates at a concentration of 500,000 cells/mL with 1 mL/well in ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies Inc, cat#10981) supplemented with 10 ng/mL recombinant IL-2 (StemCell Technologies Inc ...
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bioRxiv - Immunology 2020Quote: ... 1 x 105 T cells purified from spleen and lymph nodes with the EasySep Mouse T cell Isolation Kit (Stem Cell Technologies; Vancouver, BC, Canada) were cultured with 4 x 105 T-depleted splenocytes in 96-well round bottom plates in R10 media with 1 μg/mL αCD3 ...
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bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)
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bioRxiv - Cell Biology 2023Quote: ... and the picked colonies were allowed to grow larger and they ‘passaged’ by mechanical splitting into a new Matrigel coated plate containing mTeSR™1 media with its supplement(Anonymous, n.d.) and maintained as suggested by the manufacturer (StemCell Technologies, Canada Cat. No. #85850). Subsequent passages were done by enzymatic dissociation (Accutase® ...
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bioRxiv - Cell Biology 2023Quote: Transduced lineage negative (Lin-) or Lin-Sca-1+Kit+ (LSK) cells were plated into 1mL of methylcellulose-based medium with cytokines (Stem Cell Technology, MethoCult GF M3434) into 35 x 10 mm non-adherent Bio-One Petri Dish ...
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bioRxiv - Cell Biology 2022Quote: WIBR3 (NIHhESC-10-0079) hESCs and isogenic LIS1+/- 10 were grown and maintained on irradiated MEFs in optimal naive NHSM conditions (RSET-Stem Cell Technologies INC, supplementary Table 1) 90 ...
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cells from each experimental group were transferred to a single 35 mm culture dish containing 1 mL of MethoCult GF M3534 culture medium (Methylcellulose medium with recombinant cytokines for mouse cells, Stem Cell Technologies, Vancouver, CA, catalog no. 03534) using a 3 mL syringe and 16-gauge needle ...
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bioRxiv - Developmental Biology 2023Quote: ... Explants were then cultured in suspension in DMEM for 2 hours at 37°C with a 1/250 dilution from 100 mM stock solution or Dispase II (Stem Cell Technologies; #07923, at 0.2 U/mL).