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Citations for Stemcell Technologies :
1601 - 1650 of 1768 citations for Human Immunodeficiency Virus Nef Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured on Matrigel-coated dishes using either mTeSR-1 or TeSR-E8 medium (Stem Cell Technologies). The medium was replaced daily until cells get 70-80% confluence ...
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bioRxiv - Cancer Biology 2022Quote: ... The organoids were passage through shear stress with cold 1 U/ml Dispase/DMEM/F12 solution (StemCell Technologies) followed by Trypsin-EDTA (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... MEF feeders were inactivated at passage 4 with chemical treatment of 1 μg/ml mitomycin-c (STEMCELL Technologies) overnight ...
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bioRxiv - Bioengineering 2024Quote: ... 500 and 1500 CD34+ cells were added to 1 mL of MethoCult™ H4034 Optimum medium (StemCell Technologies) and placed into one well of a 6-well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 1×10^5 cell per ml were plated in triplicate into M3234 incomplete methylcellulose medium (StemCell Technologies) supplemented with 0.1 ng/mL GM-CSF (Peprotech) ...
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bioRxiv - Immunology 2022Quote: ... HSPCs (Figure 1 – figure supplement 1a) were sorted and transduced with the barcode library in StemSpanMedium SFEM (STEMCELL Technologies) with 50 ng/ml mSCF (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... with medium changes every 2-3 days and passages 1-2 times per week using ReLeSR (Stem Cell Technologies). Heteroplasmy levels were regularly measured to insure they were retained across passage numbers ...
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bioRxiv - Biochemistry 2020Quote: ... In short, TJP1-GFP(ZO-1-GFP) WTCs (Allen Institute, Seattle, WA) were plated in TeSR (Stem Cell Technologies) at 200,000 cells/well of Matrigel-coated 24-well plates on day (−1) ...
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bioRxiv - Neuroscience 2020Quote: Differentiation into cerebral organoids was performed with a commercially available differentiation kit from Stemcell Technologies (Catalog number 08570-1) based on previously reported protocols [8 ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture for knockout experiments.
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bioRxiv - Neuroscience 2020Quote: ... Media was changed daily and cells were split at a 1:6 ratio using Collagenase IV (Stemcell Technologies, #07909) every 5-6 days.
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bioRxiv - Cancer Biology 2021Quote: ... Following enzymatic digestion of tumor tissues using 1x Collagenase/Hyaluronidase and DNase-1 (Cat # 07912 & 07900; Stem cell technologies), dissociated tumor cells were collected in a different tube and filtered through 40 µ strainer ...
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bioRxiv - Neuroscience 2023Quote: ... The Plating medium was consisted of 1 × BrainPhys Neuronal Medium/N2-A/SM1 kit (BrainPhys; Stem Cell Technologies, 05793) supplemented with 10 μM Y27632 ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was replaced with 1 mL of BrainPhys neuronal medium containing SM1 neuronal supplement (ST-05792, STEMCELL technologies) and Human iPSC-derived mature astrocytes (XCL-1 mature astrocytes ...
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bioRxiv - Cancer Biology 2024Quote: 500 transduced MDA-MB-436 cells were mixed with 1 ml of methylcellulose (MethoCult H4034 Optimum, Stem Cell Technologies) in a 6-well cell culture plate and cultured at 37 °C in a 5% CO2 incubator ...
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bioRxiv - Bioengineering 2024Quote: HSPCs were plated at a concentration of 1 × 103 cells/ml in a methylcellulose-containing medium (GFH4435, STEMCELL Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
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bioRxiv - Immunology 2024Quote: ... T cells were plated at 1 million cells/mL in either ImmunoCult XF T cell Expansion Medium (STEMCELL Technologies) supplemented with 200U/mL IL-2 (Peprotech ...
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bioRxiv - Systems Biology 2023Quote: ... TRA-1-60) as well as differentiation into the three germ layers (STEMdiff Trilineage Differentiation kit, #05230, StemCell Technologies).
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended at a concentration of 1×106 cells/mL in neuron media [Brainphys (StemCell Technologies #05790), 1×N2 (ThermoFisher #17502-048) ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were cultured at ∼1-2 x 106mL in ImmunoCult-XF T cell expansion medium (StemCell Technologies, Canada) supplemented with Penicillin-Streptomycin (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... 1% (v/v) B27™ minus insulin) supplemented with 5 µM CHIR99021 and 33.3 ng Activin A (STEMCELL Technologies). Medium was exchanged daily without CHIR99021 supplementation ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then incubated with CD45-APC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60018AZ) and CD11B-FITC (1 µl per 50,000 cells in 100 µL ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR; Stem Cell Technologies, 05872). Cells were checked for mycoplasma contamination every two weeks ...
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bioRxiv - Molecular Biology 2023Quote: ... hPSC-generated NPCs (passage 1) were maintained and propagated using complete STEMdiff™ Neural Progenitor Medium (Stem Cell Technologies). Cells from passages 1 to 6 were cryopreserved.
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bioRxiv - Cell Biology 2023Quote: Mature organoids are collected by rinsing the maintenance well using 1-2 ml cold DMEM/F12 (Stem Cell Technologies) + 15 mM HEPES + 1x GlutaMax (1:100 35050-038 ...
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bioRxiv - Neuroscience 2024Quote: ... the plating medium was removed and replaced by Neurobasal medium supplemented with SM1 supplement (StemCell Technologies; 1:50 dilution), 0.5 mM glutamine and 0.12 mg/mL gentamycin ...
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bioRxiv - Immunology 2021Quote: ... inguinal lymph nodes from immunized or naïve mice or live LN sections were incubated in 1 mL of digestion solution comprised of RPMI1640 medium containing 0.8 U/mL Dispase (07913, Stemcell Technologies), 0.1 mg/mL Collagenase D (11088858001 ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumors were mechanically dissociated and digested for 1 h with 1x collagenase/hyaluronidase (10X Solution, Stem Cell Technology, Cambridge, MA) containing DNase I (2 U/ml ...
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bioRxiv - Cell Biology 2021Quote: ... Rescue of epithelial integrity was induced by threating hiPSCs for 1 day with LPA (StemCell Technologies, ref. 72694, 5 µM). Chemical disruption of epithelial integrity was induced by threating hiPSCs for 1 day with Blebbistatin (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged (350 x g for 5 minutes) and resuspended in 1 mL of prewarmed dispase (#07913, Stemcell Technologies) and 100 μL of DNase I (#07900 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... The yolk sac embryoid bodies (YS-EBs) were generated by treating the YS-EBs with mTeSR 1 media (STEMCELL Technologies) supplemented with bone morphogenetic protein 4 (BMP4 ...
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bioRxiv - Cancer Biology 2021Quote: CD34+ cells isolated from MF patients were plated at a density of 500 cells/replicate in 30-mm dishes containing 1 mL of StemSpan SFEM with 1.1% methylcellulose (Cat# H4230, StemCell Technologies), to which SCF ...
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bioRxiv - Cancer Biology 2020Quote: ... and the cells then dissociated in 2.5 mg/ml trypsin with 1 mM EDTA and 5 mg/ml dispase (STEMCELL Technologies) with 100 μg/ml DNaseI (Sigma ...
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bioRxiv - Immunology 2020Quote: ... and cells from wells with reactive supernatants were expanded to 48-well plates for 1 week in 0.5 mL of StemCell medium E (StemCell Technologies), before being screened again by ELISA ...
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bioRxiv - Biophysics 2020Quote: ... The cells were resuspended in 1 mL of medium contained 1x Stem Span Cytokine Cocktail CC100 (Stem Cell Technologies, Canada) in 24-well plate ...
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bioRxiv - Cell Biology 2020Quote: ... the iPSC were maintained in StemFlex medium and passaged 1-2 a week using ReLSR (STEMCELL Technologies, Vancouver, BC, Canada) dissociation buffer ...
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bioRxiv - Cell Biology 2021Quote: ... derived from different mice using a previously described method23) and selected for genomic integration using puromycin (2.5 μg mL-1; StemCell Technologies) using established procedures28 ...
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bioRxiv - Genomics 2020Quote: NSTcPA buffer (1 mL) was pipetted into one well of a 6-well plate (Stem Cell Technologies, cat. no. 38015) on ice ...
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bioRxiv - Immunology 2022Quote: ... and cells from wells with reactive supernatants were expanded to 48-well plates for 1 week in 0.5 mL of StemCell medium E (StemCell Technologies), before being screened again by ELISA ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were maintained in NbM for 6 days after which they were passaged with 1 U/mL Dispase (StemCell Technologies) and cultured in NbM supplemented with 0.1 µM Retinoic Acid (RA ...
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bioRxiv - Immunology 2024Quote: ... Freshly harvested organ tissue from male and female bats was washed in PBS and cut into 3-5 mm small pieces before placing up to four individual pieces into a 1 ml cryostorage tube containing 0.5ml-1ml of Cryostore10 freezing medium (Stemcell Technologies). Vials were immediately transferred to a cryostorage container (Mr ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 of sorted lentivirally transduced CD34+ HSPCs were seeded into 1 mL of MethoCultTM H4434 Classic media (STEMCELL Technologies #04434) supplemented with StemSpan™ CC100 with antibiotics ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and incubated with CD45-APC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60018AZ) and CD34-PE (1 µl per 50,000 cells in 100 µL ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated overnight at 4°C with primary antibody (beta-tubulin III 1:500; STEMCELL Technologies 60052) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The yolk sac embryoid bodies (YS-EBs) were generated by treating the YS-EBs with mTeSR 1 media (STEMCELL Technologies) supplemented with bone morphogenetic protein 4 (BMP4 ...
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bioRxiv - Cell Biology 2024Quote: ... CS-001) were cultured on Matrigel-coated plates in feeder-free conditions in mTeSR-1 medium (Stemcell Technologies, Vancouver, Canada). As schematized in Fig ...
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bioRxiv - Cancer Biology 2021Quote: ... Patient sample mononuclear cells were cultured in RPMI with 10% FBS and 50% HS-5 conditioned media (ATCC) or SFEMII supplemented with 1x StemSpan CD34+ Expansion Media and 1 uM UM729 (StemCell Technologies).
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bioRxiv - Genomics 2021Quote: ... they were seeded at approximately 30% confluency in Matrigel-coated culture dishes and cultured in mTeSR™1 (85851, STEMCELL Technologies) until cells fully adhered to the plate (at least 3 hours) ...
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bioRxiv - Genetics 2019Quote: ... Following six PBS platelet removal washes these were resuspended at 1×106 cells/ml in phase one media: Stemspan medium (Stemcell Tech), 1μg /ml cyclosporin A (Sigma) ...