Labshake search
Citations for Stemcell Technologies :
1651 - 1700 of 2020 citations for Cow Fibrinogen Like Protein 1 FGL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... hPSCs were dissociated to single cells in Dispase (1 U/mL, Catalog #07923, STEMCELL Technologies), and quickly reaggregated using low-cell-adhesion-coated 96-well plates with V-bottomed conical wells (Corning ...
-
bioRxiv - Genomics 2022Quote: ... H1 cells were cultured in Matrigel (Corning)-coated plates at 37°C and 5% CO2 using mTeSR-1 Basal Medium (STEMCELL Technologies) exchanged every 24 hours ...
-
bioRxiv - Genetics 2022Quote: ... and routinely passaged 1:10 every 5 days using Gentle Cell Dissociation Reagent (Stemcell Technologies).
-
bioRxiv - Immunology 2021Quote: ... 10,000 sorted cells were plated in 1 ml MethoCultTM M3630 (STEMCELL Technologies, Catalogue no.-03630) containing 2% PenStrep ...
-
bioRxiv - Neuroscience 2022Quote: ... IPSCs were cleaned and 1/3 dish was gently dissociated with dispase (Stemcell Technologies # 07923) for 12-15 minutes at 37°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... with 1:800 dilution of membrane permeant NeuroFluor™NeuO probe (cat# 01801, StemCell technologies) to detect neurons and 1:500 dilution of cell permeable DNA dye Vybrant® DyeCycle™ Ruby Stain (cat# V10309 ...
-
bioRxiv - Immunology 2021Quote: ... Cells were incubated with normal rat serum (50 µl/ 1×108 via.cells, Stem Cell Technologies and Jackson ImmunoResearch Laboratories ...
-
bioRxiv - Bioengineering 2020Quote: ... we then topped the organoids with 1 mL of fresh BrainPhys neuronal medium (Stemcell Technologies) and incubated them in a 37 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... Multiple subclones of iPS cell lines were expanded on mTeSR™1 (Stem Cell Technologies) and Matrigel (BD Biosciences ...
-
bioRxiv - Genomics 2021Quote: ... H1 cells were cultured in Matrigel (Corning)-coated plates at 37°C and 5% CO2 using mTeSR-1 Basal Medium (STEMCELL Technologies) exchanged every 24 hours.
-
bioRxiv - Cancer Biology 2020Quote: Cells were seeded (between 5×103 and 1×104) in methylcellulose (Methocult H4100, StemCell Technologies) in triplicate ...
-
bioRxiv - Cell Biology 2020Quote: ... replaced with 1 ml of BrainPhys Neuronal Medium supplemented with N2 Supplement-A (StemCell Technologies) and B27 supplement minus vitamin A (N2 / B27 BrainPhys Neuronal Medium) ...
-
bioRxiv - Physiology 2022Quote: ... Selected cells (1 × 105 cells/ml) were expanded in StemSpan H3000 media (StemCell Technologies Inc) supplemented with StemSpam CC100 (StemCell Technologies Inc ...
-
bioRxiv - Neuroscience 2022Quote: ... and were grown for 1 day with STEMdiff Neuronal induction medium (STEMCELL Technologies catalog # 05835), supplemented with 10 μM ROCK inhibitor (Y-27632 dihydrochloride ...
-
bioRxiv - Neuroscience 2022Quote: ... Adherent qNSCs were lifted from the plate using 1 mL of Accutase (STEMCELL Technologies, 07920) incubated for 15 min at 37°C and adherent aNSCs/NPCs were lifted from the plate using 1 mL of Accutase (STEMCELL Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... A full-media change with StemFlex supplemented with CloneRTM (Stemcell Technologies; 05888; 1:10 dilution) was performed once ...
-
bioRxiv - Neuroscience 2023Quote: At day -1 hiPSCs expanded in 10cm dishes were dissociated with accutase (StemCell Technologies, #07920), washed and pelleted with a 1:4 ratio of accutase to DMEM for 5 min at 1000 rcf ...
-
bioRxiv - Neuroscience 2023Quote: ... EBs were treated for 1 hour with neural rosette selection reagent (05832,Stem Cell Technologies) prior to collection and replating into matrigel-coated 12-well plastic culture plates to establish passage 0 (p0 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2×103 cells were resuspended in 1 ml of complete MammoCultTM Basal Medium (Stemcell Tech) and seeded in ultralow attachment plates ...
-
bioRxiv - Neuroscience 2023Quote: ... and CD11B-FITC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60040FI) antibodies for 15 minutes in the dark at 4C ...
-
bioRxiv - Immunology 2023Quote: ... the cell pellet was resuspended in 1 mL of Cryostor CS10 (Stemcell Technologies Catalog #07930) and transferred to a cryotube ...
-
bioRxiv - Cancer Biology 2023Quote: ... H1 cells were cultured in Matrigel (Corning)-coated plates at 37 °C and 5% CO2 using mTeSR-1 Basal Medium (STEMCELL Technologies) exchanged every 24 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... and CD34-PE (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60119PE) antibodies for 15 minutes in the dark at 4C ...
-
bioRxiv - Developmental Biology 2023Quote: ... Our hiPSC lines were cultivated under sterile conditions in mTeSR-1 (Stem Cell Technologies, Vancouver), changed daily ...
-
bioRxiv - Synthetic Biology 2024Quote: ... CD34+ HSPCs were cultured at 1-5×105 cells/mL in StemSpan SFEMII (STEMCELL Technologies) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM ...
-
bioRxiv - Neuroscience 2024Quote: ... and routinely passaged 1:10 every 5 days using Gentle Cell Dissociation Reagent (StemCell Technologies).
-
bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated from WT mice by magnetic bead negative selection using EasySep Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 media supplemented with 10% FBS ...
-
bioRxiv - Developmental Biology 2021Quote: Neuroectodermal organoids were prepared using hiPS cells expressing OptoShroom3 following the first steps of cerebral organoid protocol53 with STEMdiff™ Cerebral Organoid Kit (STEMCELL Technologies, #08570). The modifications from commercial protocol were ...
-
bioRxiv - Biophysics 2022Quote: ... Human neutrophils were isolated from whole blood of healthy donors by immunomagnetic negative selection using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Isolation began from an initial blood volume of 0.5-1 ml ...
-
bioRxiv - Immunology 2019Quote: ... CD4+ cells were separately isolated using mouse CD4+ T-cell negative selection protocol (EasySep™ Mouse CD4+ T-cell Enrichment Kit, Stem Cell Technologies) to a purity of > 90% according to the manufactureŕs instructions ...
-
bioRxiv - Bioengineering 2019Quote: ... Initial HSPC isolation was performed using defined protocols of EasySep™ Mouse Hematopoietic Progenitor Cell Enrichment Kit and Magnet (#19756, #18000, Stemcell Technologies, CA). A secondary isolation further enriched the HSPC population by collecting the Lin− Sca-1+ c-kit+ (LSK ...
-
bioRxiv - Cancer Biology 2021Quote: Human primary total T cells (CD4 and CD8) were isolated from normal healthy donors following leukapheresis by negative selection using RosetteSep kits (Stemcell Technologies, Vancouver, CA). All specimens were collected with protocol approved by University Review Board and written informed consent was obtained from each donor ...
-
bioRxiv - Bioengineering 2021Quote: ... and CD34+ cells were isolated using EasySep™ CD34 positive selection kit II (samples from Basel, Cat# 17896, Stemcell Technologies, Vancouver, BC, Canada).
-
bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
-
bioRxiv - Microbiology 2021Quote: ... were generated by positive selection of CD14+ cells from PBMCs using the EasySep Human CD14 Positive Selection Kit II (STEMCELL Technologies, Cat: 17858) and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma) ...
-
bioRxiv - Microbiology 2021Quote: ... we first isolated CD4+ T cells from 10M PBMC by negative selection using the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies, Cat #: 19052). This yielded a mean of 1.6 (range 1.3-2.0 ...
-
bioRxiv - Cancer Biology 2020Quote: ... from wild-type or Jak1 floxed C57/Bl6 mice were isolated using EasySep mouse hematopoietic progenitor cell isolation kit (Stemcell technologies, Vancouver, Canada) which remove lineage marker-expressing cells with biotinylated antibodies directed against non-hematopoietic stem cells and non-progenitor cells (CD5 ...
-
bioRxiv - Cancer Biology 2022Quote: ... To make single-cell suspensions for re-plating, the spheres are chemically dissociated after 7 days of plating, using NeuroCult™ Chemical Dissociation Kit (Mouse, #05707) from Stem Cell Technologies according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2022Quote: Preparation of cells: Human CD4+ cells were isolated from buffy coats using EasySep™ Human CD4+ T Cell Isolation Kit (Stemcell Tech, # 17952), resuspended with complete RPMI (cRPMI ...
-
bioRxiv - Neuroscience 2021Quote: ... 24 hours before each recording media was changed to supplemented BrainPhys™ Neuronal Medium and SM1 Kit (STEMCELL Technologies SARL, cat no. #05792). Axion Integrated Studio (AxIS 2.1 ...
-
bioRxiv - Neuroscience 2021Quote: ... following a monolayer culture method with a commercial dual SMAD inhibition-mediated neural induction medium (STEMdiff™ SMADi Neural Induction Kit, Stem Cell Technologies) based on a published protocol59 ...
-
bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
-
bioRxiv - Immunology 2022Quote: ... erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep™ Rat CD4+ T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting ...
-
bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated by negative magnetic selection from either B*08:01+ or B*35:01+ donor blood using kits from StemCell Technologies (catalog #17953). To screen for antigen-specific CTLs ...
-
bioRxiv - Cancer Biology 2019Quote: ... 1×107/ml of PBMCs were used for isolation of CD4+ CD127low CD25+ Regulatory T cells using the EasySep™ CD4+ CD127low CD25+ Human Regulatory T Cell Isolation Kit (STEMCELL Technologies, MA) following the manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were first enriched for CD8 T cells using EasySep™ human total or naïve CD8 T Cell enrichment kits (STEMCELL Technologies, Vancouver, Canada). CD56− and CD56+ PBMC fractions were sorted by flow cytometry ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
-
bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...
-
bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
-
bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from spleen and peripheral lymph nodes from indicated mice by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies Inc). 1.75 x 105 cells were cultured for 4 days in RPMI1640 supplemented with 10% heat-inactivated FCS ...