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Citations for Stemcell Technologies :
1501 - 1550 of 2020 citations for Cow Fibrinogen Like Protein 1 FGL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: CD8 T cells were isolated from the lymph node of naïve mice with unactivated Confetti cassette using EasySep™ Mouse CD8+ T Cell Isolation Kit (StemCell Technologies) and macrophages were sorted from the tumors via FACS sorting ...
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bioRxiv - Immunology 2023Quote: Small resting B cells were isolated from spleen with a discontinuous Percoll gradient and subsequent purification using EasySep Mouse B cell Isolation Kit (StemCell Technologies). B cells were stimulated with 3mM CpG (Sigma) ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from peripheral lymph nodes (inguinal, axillary, brachial and superficial cervical) by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies). CD4+ T cells were stimulated in 96 well flat-bottomed plates coated with 20mg/ml αCD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2024Quote: ... OT-I+/- or total CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection using the EasySepTM Mouse CD8+ T cell isolation kit (#19753, StemCell Technologies). For in vitro experiments ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were isolated from PBMCs by CD14 positive selection using the EasySep™ Human CD14 Positive Selection Kit (18058; Stemcell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... CD4+ CD45RO+ memory T cells were then isolated by negative selection using an EasySepTM Human Memory CD4+ T Cell Enrichment Kit (STEMCELL Technologies).
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bioRxiv - Cancer Biology 2024Quote: NK cells were isolated from PBMCs of healthy donors by depleting CD3 cells using an EasySep human CD3 positive selection kit II (StemCell Technologies). The negative cell fraction was expanded using irradiated (100 Gy ...
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bioRxiv - Immunology 2024Quote: ... The resulting hybridoma cells were selected on semi-solid methylcellulose and grown in 96-well plates using the ClonaCell-HY Hybridoma Cloning Kit (Stemcell Technologies). Hybridomas secreting SH-01-reactive IgG were identified by several rounds of ELISA screening ...
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bioRxiv - Cancer Biology 2024Quote: Primary mouse NK cells were isolated from spleens of 8-12-week-old wild-type or Prf1-/- mice using the negative selection EasySep mouse NK cell isolation kit (STEMCELL Technologies) or positive selection CD49b (DX5 ...
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bioRxiv - Cancer Biology 2024Quote: The ALDH activity of oral cancer cells co-cultured with CAF was measured using the ALDEFLUOR assay kit (Cat. #01700, StemCell Technology), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... CD14+ monocytes were then isolated from the PBMC fraction using the EasySep™ Human CD14 Positive Selection Kit II (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... mTESR plus medium was replaced with STEMdiff™ Hematopoietic Supplement A medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). On day 3 ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... FGF10 (each 100 ng/ml) and IWP4 (1 µM) were obtained from StemCell Technologies. The PKC activator PDBu was purchased from Tocris (biotechne ...
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bioRxiv - Cell Biology 2022Quote: ... 500 CD34+ HSPCs were plated in 1 ml methylcellulose media (H4034, Stem Cell Technologies) in triplicate unless otherwise noted ...
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bioRxiv - Neuroscience 2021Quote: ... at 37 °C for 1 min and seeded on AggreWell 800 (#34815; StemCell Technologies) in Essential 8 medium with ROCK inhibitor Y-27632 (10 μM ...
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bioRxiv - Genomics 2020Quote: ... Aspirated off the supernatant and resuspended pellet in 1 ml Accumax (Stemcell Technologies, 07921) for 3 min at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... iPSCs were cultured in mTeSR-1 medium on Matrigel coated plates (Stem Cell Technologies) and passaged into a single cell suspension using Accutase (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: ... and interleukin-3 (IL-3; 1 ng/mL, first day only; Stemcell Technologies; Canada). Cell density was maintained between 0.7-2×106 cells/mL by daily feeding with fresh expansion medium.
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 1% N2 supplement and 2% NeuroCult S1 supplement (STEMCELL Technologies, Vancouver, BC). After one day media was removed and replaced with a thin layer of phosphate buffered saline ...
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bioRxiv - Neuroscience 2021Quote: Induced pluripotent stem cells were grown using mTeSR™1 medium (Stemcell Tech., 05850) on matrigel (Corning ...
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bioRxiv - Neuroscience 2020Quote: ... Dibutyryl-cAMP (1 mM) and ascorbic acid (200 nM) (STEMCELL Technologies, Vancouver, BC, Canada). Half fresh medium containing Amyloid β Protein Fragment 1-42 (50 nM ...
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bioRxiv - Microbiology 2021Quote: ... and samples were resuspended in 1 mL PneumaCult media (PneumaCult-Ex, 05008; Stemcell Technologies) with 1% penicillin/streptomycin mixture (17-602E ...
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bioRxiv - Developmental Biology 2022Quote: ... 5 ng/mL FGF-2 (RnD Systems) and 1 µM CHIR99021 (Stem Cell Technologies) with daily medium exchange for 3 days ...
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bioRxiv - Neuroscience 2022Quote: Human iPSC (XCL-1) derived neural progenitor cells (NPCs, Stem Cell Tech cat#70901) were maintained in serum-free STEMdiff™ Neural Progenitor Medium 2 (Stem Cell Tech cat#08560 ...
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bioRxiv - Genomics 2020Quote: ... iPSCs were adapted from DEF-CS culturing system to mTeSR™1 (STEMcell Technologies) culturing system by seeding 40K/cm2 on Geltrex (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... maintained in feeder-free conditions in a mTeSR™1 (85850; Stem Cell Technologies) medium supplemented with 1% Penicillin-Streptomycin (P0781 ...
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bioRxiv - Developmental Biology 2020Quote: ... HiPSCs were adapted and maintained with mTeSR™1 culture medium (05850, Stemcell Technologies) on Corning® Matrigel® Basement Membrane Matrix ...
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bioRxiv - Cell Biology 2021Quote: ... 5 ng/ml FGF-2 (RnD Systems) and 1 μM CHIR99021 (Stem Cell Technologies). Mesoderm induction required daily medium exchanges for 3 days ...
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bioRxiv - Bioengineering 2021Quote: ... The three hiPSC lines were maintained in mTeSR™1 medium (STEMCELL Technologies # 85850), in 6-well tissue culture plates (Falcon ...
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bioRxiv - Neuroscience 2021Quote: ... Isotype control was stained using control antibody (STEMCELL Technologies, 60070AD.1, 0.2 ug/ul) at 1:378 dilution for 10 min at 4 °C protected from light ...
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bioRxiv - Microbiology 2022Quote: ... 1% Penicillin/Streptomycin and 16 ng/ml hepatocyte growth factor (HGF, Stem Cell Technologies). When selecting cells transduced by lentivirus ...
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bioRxiv - Neuroscience 2022Quote: ... NPCs are ready for passage 1 at approximately day 17-19 (Accutase, StemCell Technologies). NPCs were plated at 1.25×5 cells/cm2 on Matrigel hESC-qualified Matrix-coated wells and maintained in Neural Progenitor medium (StemCell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Digested PDX tumor cells were treated with 1 mg/mL DNase I (Stemcell Technologies) for 1-2 minutes until sample lost viscosity ...
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bioRxiv - Cell Biology 2023Quote: ... Erythrocytes were lyzed by mixing with ammonium chloride (1:10 v/v; StemCell Technologies) at 4°C for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... Feeder-free hiPSCs were routinely maintained on Matrigel in mTeSR 1 medium (StemCell Technologies). Differentiation of hiPSCs to hNPCs was started with induction of neurospheres in aggrewell plates (StemCell Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... The following primary antibodies were used: beta-tubulin III (STEMCELL Technologies 60052; 1:1000), GFAP (Dako GA524 ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated in 1× Collagenase/Hyaluronidase and 125 Units/mL DNase (Stem Cell Technologies) with frequent pipetting at 37 °C for two hours ...
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bioRxiv - Neuroscience 2023Quote: GNAO1+/G203R#2 hiPSCs were cultured in mTeSR™1 medium (Stem Cell Technologies) on Matrigel (Corning)-coated plates following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: hiPSCs were cultured in mTeSR™1 plus supplement (Stem Cell Technologies, Cat. #85850) on a 6-well plate coated with hESC-qualified Matrigel (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 48 hours of 5 µM IWR-1-endo (Stem Cell Technologies 72562) on day 4 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc., Canada) and FACS on a BD FACSAriaTM II using the following markers/staining ...
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bioRxiv - Immunology 2021Quote: OVA (SIINFEKL)-specific CD8+ T cells were isolated from the spleen and lymph nodes of healthy 6-8-week old OT-I mice using EasySep™ Mouse CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: Murine T-cells were isolated from spleens of healthy 6-8-week old C57BL/6 mice using EasySep™ Mouse CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... CECs were isolated from human bone marrow aspirates or PBMC derived CECs culture using EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... CD4+ or CD8+ T-Cells were isolated from PBMC using EasySep™ Human CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: Primary NK cells were isolated from peripheral blood lymphocytes (PBLs) of healthy donors using the EasySep™ human NK Cell enrichment kit (STEMCELL Technologies). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... cells were centrifuged at 500 x g for 15 min at room temperature and were resuspended in RoboSep buffer for B cells isolation by negative selection with EasySep™ Human B Cell Isolation Kit (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: HSPCs were purified from discarded umbilical cord blood samples of healthy male or female newborns using the EasySep Human CD34 Positive Selection Kit II following pre-enrichment using the RosetteSep Pre-enrichment cocktail (Stem Cell Technologies) and mononuclear cell isolation on Ficoll-Paque (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: Total CD8+ T cells were isolated from the peripheral blood of 2 healthy donors using EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies). Isolated CD8+T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 beads (Invitrogen ...