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Citations for Stemcell Technologies :
1601 - 1648 of 1648 citations for Mono 2 Ethyl 5 Hydroxyhexyl Phthalate 13C4 99% Dehp Metabolite Ix 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Human splenocytes were then cultured at 1×106 cell per mL in ImmunoCult-XF T cell Expansion Medium (Stemcell Technologies, Vancouver, BC). For T cell stimulation ...
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bioRxiv - Cancer Biology 2020Quote: ... Each fragment was placed in 1 mL of freezing media (400 μL of NeuroCult NS-A Basal medium with proliferation supplement (StemCell Technologies; #05751) containing 20 μg/mL rhEGF (Peprotech ...
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bioRxiv - Genetics 2021Quote: ... Cells were resuspended in 1 mL pre-warmed Neuronal Expansion Media with Rock inhibitor (Y-27632 compound, Stem Cell Technologies, cat# 72304) at a final concentration of 10 μM and a cell count performed ...
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bioRxiv - Immunology 2021Quote: ... T cells at a concentration of 2e6/ml were re-stimulated with ImmunoCult™ Human CD3/CD28/CD2 (Stemcell Technologies cat 10970) at 6.25μl/ml ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 15 min at 37°C and adherent aNSCs/NPCs were lifted from the plate using 1 mL of Accutase (STEMCELL Technologies, 07920) incubated for 5 min at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then resuspended at 1 million/mL in either Immunocult-XF T-cell Expansion Medium (Cat # 10981, STEMCELL Technologies, Vancouver, Canada) or TEXMACs Cell Culture Medium (Cat # 130-097-196 ...
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bioRxiv - Neuroscience 2024Quote: ... the cell pellet was mixed gently with STEMdiff™ Neural Induction Medium (NIM)+1µl/ml ROCK inhibitor Y-27632 (#72302; Stem Cell Technology) and plated at 3×106 cell density per well into Nunclon Sphera 96 U bottom plates (#174929 ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue was finely minced and placed in 15 ml conical tubes with a dissociation solution composed of Collagenase/Hyaluronidase (Stem Cell Technologies) enzymes ...
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bioRxiv - Bioengineering 2024Quote: ... Dissociation was performed by incubation in dissociation solution (1:1 dilution of 2.5% Trypsin (GibcoTM, Cat#: 15090046) and 1 U/ml Dispase (Stemcell Technologies, Cat#: 15569185)) for 10 min under gentle shaking and occasional gentle pipetting at 28°C ...
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bioRxiv - Developmental Biology 2022Quote: ... the media was changed to pre-warmed 37°C mesoderm / Endothelial “priming” media (8 uM CHIR99021 +25 ng/ml BMP4, 1× Glutamax, 1× N2, 1× B27, Neurobasal, DMEM/F12, Stem Cell Technologies). One well of seeded cells was left untreated as negative control in StemFlex (data not shown) ...
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bioRxiv - Immunology 2023Quote: ... were then seeded overnight on mIL-1β capture antibody coated-coverslips in cRPMI medium supplemented with 10 ng/mL hrM-CSF (Stem Cell Technologies). 30 min before harvesting Mitotracker DeepRed (MTDR ...
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bioRxiv - Developmental Biology 2023Quote: ... After 3 days of growth of naïve PSC were harvested and counted and 55 cells were seeded per microwell (total of 66,000 cells were seeded per 24-well in 1 ml of medium) AggreWell 400 24-well (Stemcell Technologies cat 34415) in N2B27-BSA supplemented with 10 µM ROCKi Y27632 ...
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bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6low and CCR6high memory subgroups and cultured at 1 × 106 cells/ml in 24 well plates in ImmunoCult XF T cell expansion medium (Stem Cell Technologies) supplemented with 10% FBS and IL-2 (200 units/ml) ...
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bioRxiv - Cell Biology 2023Quote: ... Accutase was quenched and single cells were seeded for differentiation in suspension at a density of 1×106 cells/mL in complete mTeSR media (Stem Cell Technologies) supplemented with 10ng/mL FGF2 (Peprotech ...
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bioRxiv - Cell Biology 2023Quote: ... final concentrations of either 3 µM CHIR99021 for definitive hematopoietic differentiation or 3 µM IWP2 (Selleckchem, S7085) and 1 ng/ml activin A (Stemcell Technologies, 78001) for primitive hematopoietic differentiation were added to the media for 30 hours ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... Alomone Labs), 10 ng/mL brain-derived neurotrophic factor (167450-02-STD, tebu-bio) and 500 µM dibutyryl-cAMP (Stemcell Technologies, 73886) and motoneurons were maintained for 25 DIV.
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bioRxiv - Synthetic Biology 2023Quote: ... T cells were then resuspended at 1×106 cells/mL in ImmunoCult™-XF T Cell Expansion Medium (Stemcell Technologies, Cat.#10981) supplemented with Human Recombinant IL-2 (12.5 ng/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... Mouse bone marrow c-Kit+ cells from Rosa26-LSL-Cas9 knockin mice were transduced with the sgRNAs using the lentivirus and were selected for stable expression of the sgRNAs using puromycin (1 μg/ml) in MethoCult™ M3234 (STEMCELL Technologies) supplemented with 10 ng/mL mouse SCF ...
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bioRxiv - Cancer Biology 2021Quote: ... ∼800 CD34+ gene-edited and control cells were mixed with 1.0 mL of MethoCult™ SF H4636 methylcellulose (Stemcell Technologies, Cat. No. 04636) containing recombinant cytokines for human embryonic stem (ES ...
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bioRxiv - Immunology 2019Quote: ... and Mononuclear cells were incubated with 1 ml of erythrocytes and the RossetteSep™ CD8+T cell enrichment cocktail to obtain Total CD8+T cells (15063; StemCell Technologies, Canada). The memory cells were eliminated using magnetic beads (8803 ...
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bioRxiv - Cancer Biology 2020Quote: GFP positive neural tube regions were dissected after a DispaseI-DMEM/F-12 treatment (Stem cell technologies 1U/ml #07923; 37°C, 30min). Single cell suspensions were obtained by 3 minutes incubation in Trypsin-EDTA 0.05% (Life technologies ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were needle triturated and resuspended in Mouse NSC Proliferation Media (1x Anti-Anti, 10ng/mL bFGF (#78003, Stem Cell Tech, Vancouver), 20ng/mL mEGF (#78016 ...
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bioRxiv - Immunology 2020Quote: ... Primary human bronchial epithelial cells derived from healthy patient bronchial brushings were grown in PneumaCult ExPlus Medium supplemented with 96 μg/mL hydrocortisone (StemCell Technologies, Vancouver, BC) and 1X antimicrobial-antimycotics (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
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bioRxiv - Cancer Biology 2021Quote: ... and then removed from the Parafilm and transferred to wells of a 24-well plate (one form per well) containing 1.5 mL of neural stem cell media (NeuroCultTM; 05700, STEMCELL Technologies, Vancouver, BC, Canada) supplemented with human EGF (20 ng/mL ...
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bioRxiv - Neuroscience 2024Quote: ... media was replaced every other day with neuronal differentiation media prepared based on the Stem Cell Technology protocol: 10 ml of the BrainPhys™ Neuronal Medium (#05790; Stem Cell Technology) supplemented with 200 µl of NeuroCultTM SM1 Neuronal Supplement (#05711 ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood using density gradient centrifugation at 1,200 x g for 10 min where diluted blood was layered over 15 mL of Lymphoprep™ (STEMCELL Technologies, Vancouver, Canada) followed by harvesting of the buffy coat ...
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bioRxiv - Cancer Biology 2024Quote: ... Corning 10-092-CV; 20% fetal bovine serum [FBS], Sigma-Aldrich F4135; and 0.1 mg ml-1 DNase I, Stem Cell Technologies 07900). Cell suspension was placed in a new tube ...
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bioRxiv - Developmental Biology 2022Quote: ... 1.25 million SOX2-mTurquoise2 / NANOG-mVenus H9 cells were resuspended in 1 ml of STEMdiff™ Neural Induction Medium (NIM, Stem Cell Technologies) with STEMdiff™ SMADi Neural Induction Supplement (SMADi ...
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bioRxiv - Cell Biology 2023Quote: ... 3.3 x 105 cells and 2.6 x 105 cells per ml were seeded in 3 ml E8 medium supplemented with 10 µM Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (RI, STEMCELL Technologies, Vancouver, Canada), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Genetics 2023Quote: ... Lentivirus media was removed after 24 hours and the cells were allowed to rest for another 24 hours before being selected for 7 days with Puromycin at 8µg/ml (73342, STEMCELL Technologies, Cambridge, MA, USA). Surviving cells were expanded ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Thawed PMBCs from a vial containing around 1 × 107 cells were pre-cultured in one well of low attachment 6-well plate including 4 mL of StemSpan-AOF (Cat#ST100-0130, STEMCELL Technologies, Vancouver, Canada) supplemented with recombinant human IL-6 (100 ng/mL) ...
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bioRxiv - Bioengineering 2024Quote: ... These batch were collected in 50mL Cryostore bags and frozen in a final 50 × 106 cells/mL cell density for cryopreservation using CS10 Freeze Medium (STEMCELL Technologies; cat#07930). Once CAR T cells had been harvested in CS10 Freeze Medium ...
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bioRxiv - Bioengineering 2020Quote: MSCs at passages 1 or 3 were seeded in wells of a 12-well plate containing 3 ml Mesencult (Stem Cell Technologies, Vancouver, B.C.) each at 8.0×104 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were pelleted by centrifugation at 300 g for 10 min and resuspended at 108 cells/mL in EasySep™ buffer and B lineage cells were isolated using an EasySep™ mouse B cell negative isolation kit (StemCell Technologies, #19854). For serum ...
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bioRxiv - Immunology 2022Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×106 T cells/ml in serum-free StemCell Immunocult™-XF media (Cat#10981, StemCell Technologies, Vancouver, Canada) with 20U/ml clinical grade human IL-2 (Novartis) ...
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bioRxiv - Cancer Biology 2023Quote: ... myeloid cells were gently thawed and washed in warm myeloid cell media (ImmunoCult™-SF Macrophage Differentiation Medium - using base media with only, M-CSF 50 ng/mL | STEMCELL Technologies, Cat. 10961), and plated in a 24-well low-attachment plate (Corning ...
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bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Daily media changes of E7™ Reprogramming Media followed until the emergence of iPSC colonies between day 21-28 when iPSC colonies were picked and transferred to Geltrex™ coated 12-well plates containing 1 mL TeSR™-E8™ (STEMCELL Technologies) supplemented with 10 μM ROCK Inhibitor (Y-27632 ...
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bioRxiv - Bioengineering 2019Quote: ... SVF was plated at a cell density of 7×106 in T75 tissue culture flasks in 15 ml of MesenCult MSC basal medium (Stem Cell Technologies, Cambridge, MA, USA) supplemented with MesenCult MSC Stimulatory Supplement ...
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bioRxiv - Cell Biology 2020Quote: Flow cytometer-sorted murine or human HSCs were resuspended in 4 mL complete MethoCult GF M3434 medium or MethoCult™ H4435 Enriched (STEMCELL Technologies, 03434 and 04435) thawed at RT and 1 mL of the suspension was placed into 35 mm dishes in triplicates ...
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bioRxiv - Developmental Biology 2021Quote: ... The media was then replaced with hTSC media containing 200 ng/ml recombinant B18R to prevent gamma-interferon response (Stem Cell Technologies; Cat. No. 78075). For reprogramming experiments lipofection was performed at the same time every day for 20 days ...
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bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)
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bioRxiv - Cancer Biology 2023Quote: ... APC-eFluor780-conjugated cKit and 7AAD and purified by fluorescent activated cell sorting (FACS) at a density of 600 Lin- Sca1+cKit+7AAD- cells per 1.1 ml/well of MethoCult™ M3334 (STEMCELL Technologies, Vancouver, British Columbia, Canada). M3334 methocult was supplemented with cytokines to a final concentration of 5 ng/ml mIL-3 ...
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cells from each experimental group were transferred to a single 35 mm culture dish containing 1 mL of MethoCult GF M3534 culture medium (Methylcellulose medium with recombinant cytokines for mouse cells, Stem Cell Technologies, Vancouver, CA, catalog no. 03534) using a 3 mL syringe and 16-gauge needle ...