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Citations for Stemcell Technologies :
1251 - 1300 of 1648 citations for Mono 2 Ethyl 5 Hydroxyhexyl Phthalate 13C4 99% Dehp Metabolite Ix 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... after which 4 ml maturation medium (Cerebral organoid kit, cat. n. 08570, StemCell Technologies) were gently added to each well ...
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bioRxiv - Developmental Biology 2023Quote: ... 15 ml Falcon Tubes were rinsed with Anti-adherence Rinsing Solution (StemCell Technology, #07010) to prevent cell adhesion to the tube ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed twice with PBS after which 4.5 ml of Accutase (STEMCELL technologies) was added ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.25 μg/mL amphotericin B) supplemented with 10 μM Y-27632 (STEMCELL Technologies). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated in 1× Collagenase/Hyaluronidase and 125 Units/mL DNase (Stem Cell Technologies) with frequent pipetting at 37 °C for two hours ...
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bioRxiv - Bioengineering 2023Quote: ... 20ng/mL human plasma-derived low-density lipoprotein (hLDL) (Stemcell Technologies, Cat. No. 02698), 1uM StemReginin1 (SR1 ...
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bioRxiv - Bioengineering 2023Quote: ... The segments were then incubated in 25 mL Gentle Cell Dissociation Reagent (STEMCELL Technologies) at room temperature for 15 minutes on a rocking platform at 20 RPM ...
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bioRxiv - Neuroscience 2024Quote: ... 5.5 mM L-cysteine HCl) and 125 units/mL DNase I (07900, Stemcell technologies) in Hanks’ Balanced Salt Solution (HBSS ...
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bioRxiv - Cell Biology 2023Quote: ... On days 4 and 6 LDM medium with added 50ng/ml BMP4 (STEMCELL Technologies) and 6ul/ml DMSO was used ...
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bioRxiv - Physiology 2024Quote: ... layered carefully over 10 mls of LymophoprepTM density centrifugation medium (StemCell Technologies, Vancouver, Canada) and centrifuged at 800 x g for 20 minutes at room temperature with the brake off ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1mM ßMercaptoethanol (BMe)) and freshly added mouse recombinant LIF (1000U/ml; Stemcell technologies 78056). 2i-cultured mESCswere grown on plates coated with Fibronectin (17.6μl/1ml PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Sigma for 2 min at room temperature and then incubated in neutralization buffer supplemented with NH4Cl (0.8%, StemCell Technologies) to remove red blood cells ...
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An in vitro neuronal model replicating the in vivo maturation and heterogeneity of perineuronal netsbioRxiv - Neuroscience 2022Quote: ... the media was replaced with BrainPhys™ medium with 2% (v/v) SM1 and antibiotic-antimycotic (StemCell Technologies #5792). From 14 DIV ...
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bioRxiv - Neuroscience 2022Quote: ... neural induction was initiated using a chemically defined neural base medium (NbM) supplemented with 2 µM Dorsomorphin (StemCell Technologies), 3 µM CHIR990021 (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... with base media consisting of Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 2% human blood plasma (STEMCELL Technologies, #70039), 3% human AB serum (Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: 2-3 days post-electroporation HSPCs were plated in SmartDish 6-well plates (cat.: 27370; STEMCELL Technologies, Vancouver, Canada) containing MethoCult H4434 Classic or MethoCult H4434 Classic without EPO (cat. ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were cultured at ∼1-2 x 106mL in ImmunoCult-XF T cell expansion medium (StemCell Technologies, Canada) supplemented with Penicillin-Streptomycin (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR; Stem Cell Technologies, 05872). Cells were checked for mycoplasma contamination every two weeks ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were washed twice with PBS (+ 2%FBS) and with a red blood cell lysis buffer (0.8% NH4Cl, 0.1mM EDTA, STEMCELL technologies). Thereafter ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2 µM A83-01, an inhibitor of TGFβ type I receptors (ALK4, ALK5, ALK7) (Stem Cell Technologies, 72022) for 5 days in vitro to induce embryoid body (EB ...
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bioRxiv - Immunology 2024Quote: ... Human whole-blood samples were diluted with 2% FBS in PBS prior to gradient centrifugation on Lymphoprep (StemCell Technologies). A percentage of the freshly isolated PBMCs was labelled with anti-human CD3 ...
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bioRxiv - Developmental Biology 2022Quote: ... Stem cells were maintained as previously described (Spence et al., 2011) and grown in mTeSR Plus media (StemCell Technologies, Cat#100-0276).
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bioRxiv - Immunology 2020Quote: ... CD8+ T-cells autologous to these CD4+ targets were enriched on the day of co-culture from freshly a thawed aliquot of 100×106 PBMCs by negative selection (Easysep™, Stemcell Technologies). Infected and mock-infected CD4+ T-cells were washed 5x and then co-cultured with CD8+ T-cells at ratios of 5 CD8+ T-cells to 1 CD4+ T-cell ...
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bioRxiv - Cancer Biology 2019Quote: Single cells were FACS-isolated into flat bottomed 96-well plates containing 100 μl of MethoCultTM H4435 Enriched (StemCell Technologies Cat#04435). Colonies were visually inspected and classified 11-14 days after plating ...
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bioRxiv - Neuroscience 2019Quote: ... 8 x 105 cells were resuspended in 100 μl Ingenio-solution (Mirrus) supplemented with Rock inhibitor (10 μM, Y-27632, Stem Cell Technologies) and 2.5 μg of CRISPR:hSpCas9 plasmid and 2.5 μg linearized DNA targeting plasmid and immediately pulse with program DC100 (using a 4D-Nucleofector ...
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bioRxiv - Neuroscience 2023Quote: ... Passaging of colonies was done roughly every five days using Gentle Cell Dissociation Reagent (GCDR) from STEMCELL Technologies (Cat No. 100-0485). Stem cell pluripotency was validated using the STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2023Quote: Mature astrocytes were created from astrocyte precursor cells using the STEMdiff Astrocyte Maturation media kit from STEMCELL Technologies (Cat No. 100-0016) following their guidelines for Matrigel-coated 6-well plates ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ HSPCs were then seeded into 96-well round bottom plates (cell density at 100,000 (100k) cells/100 μL) and prestimulated in StemSpanTM SFEM (Stem Cell Technologies #09650) supplemented with StemSpan™ CC100 (Stem Cell Technologies #02690) ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged every 3-4 days by dissolving the Matrigel in Gentle Cell Dissociation Reagent (GCDR - STEMCELL Technologies cat#100-0485). Organoids were broken down to smaller sizes by trituration in GCDR before washing with basal media (advanced DMEM/F12 ...
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bioRxiv - Bioengineering 2024Quote: ... or modified Caf1 cell culture scaffolds coated 60 mm culture dishes with mTeSR™ Plus stabilised feeder-free maintenance medium (100-0276, STEMCELL Technologies) following the manufacturers’ recommendations ...
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bioRxiv - Bioengineering 2024Quote: hPSCs were maintained on Geltrex-coated plates in feeder-free mTeSR-1TM and mTeSR PlusTM media (Stemcell Technologies #85850 and #100-0276) using an established protocol (63) ...
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bioRxiv - Neuroscience 2024Quote: ... passaged through a 40 μm strainer and seeded at a low density in Matrigel-coated 100 mm dishes in mTeSR1 supplemented with 1x CloneR (Stem Cell Technologies). After 7-8 days ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C for 5 minutes and replated in a ratio of 1:6 or 1:9 on new Matrigel-coated plates in E8 supplemented with 5 μM ROCK inhibitor Y-27632 (Stemcell Technologies, Canada) for 24h ...
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bioRxiv - Genetics 2019Quote: 1×10^5 cells from the RF of xenotransplanted mice were transferred to 1ml of MethoCult H4034 Optimum methylcellulose medium (StemCell Technologies, 04034) and plated onto a 35mm dish for human specific colony formation ...
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bioRxiv - Bioengineering 2021Quote: ... we first patterned the hiPSCs with a mesenchymal protocol as previously described.(Lam, et al., 2014) Cells were treated with 5 µM CHIR99021 (STEMCELL Technologies, 72052) in Roswell Park Memorial Institute Medium 1640 (RPMI 1640 ...
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bioRxiv - Bioengineering 2021Quote: ... we first directed the hiPSCs towards an intermediate mesenchymal fate using a previously described protocol,[84] wherein cells are treated with 5 µM CHIR99021 (STEMCELL Technologies, 72052) in Roswell Park Memorial Institute Medium 1640 (RPMI 1640 ...
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bioRxiv - Neuroscience 2021Quote: ... These cells were cultured in mTeSR™1 medium (mTeSR™1 Basal Medium with mTeSR™1 5× Supplement (Stemcell Technologies) and 1% penicillin-streptomycin (P/S ...
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bioRxiv - Cell Biology 2020Quote: ... Neural rosettes were derived between day 3 to day 5 by addition of STEMdiffTM Neural Rosette Selection Reagent (05832, Stem Cell Technologies) for 45 mins-1 hr at 37oC ...
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bioRxiv - Molecular Biology 2022Quote: ... The differentiation was started following 24 h of seeding and proceeded through a 7-stage differentiation protocol (stages 1 – 4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Cell Biology 2022Quote: ... Thawed cells were cultured at 37°C and 5%O2 in serum-free HSC media comprised of StemSpan II medium (Stem Cell Technologies) supplemented with CC100 cytokine cocktail (Stem Cell Technologies) ...
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bioRxiv - Neuroscience 2019Quote: ... followed immediately by engrafting 1-5×104 human umbilical cord CD34+ stem cells (Lonza Incorporated, Walkersville, MD; or Stemcell Technologies, Vancouver, BC) via intrahepatic injection ...
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bioRxiv - Immunology 2021Quote: ... Cells were fed for 4-5 days until airlifted and continued to be grown at air-liquid interface (ALI) with PneumaCult ALI medium (StemCell Technologies, #05001) until fully differentiated (4 weeks)
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Then cells were seeded at 70% confluency on dishes pre-coated with Matrigel and cultured in StemMACS iPS-Brew XF + 5 μM Y-27632 (STEMCELL Technologies 72304). On D1 ...
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bioRxiv - Developmental Biology 2023Quote: ... and maintained at 37C with 5%CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Tec.) ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell culture medium was supplemented with 1:200 Rho-associated protein kinase inhibitor (ROCKi) Y-27632 (final concentration 5 µM, STEMCELL Technologies, 72302) on the first day after passage ...
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bioRxiv - Molecular Biology 2023Quote: E14 and RPL39L KO cells were treated for 5h at 37°C and 5% CO2 with 10μM (S)-MG132 (STEMCELL Technologies Catalog #73264) and 5μM Bafilomycin-A1 (STEMCELL Technologies Catalog #74242 ...
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bioRxiv - Bioengineering 2023Quote: ... we washed 80-90% confluent cultures with PBS-and dissociated cultures into a single-cell suspension with 5 min of Accutase (StemCell Technologies Inc.) treatment at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Single cells were centrifuged at 200g for 5 minutes and resuspended in StemFlex supplemented with 10 µM Y-27632 (StemCell Technologies Inc.). We seeded 5.5×106 cells per 5.5 mL of mTeSR+ in each well of a six-well suspension plate ...