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Citations for Stemcell Technologies :
1551 - 1600 of 1767 citations for Rat Insulin like growth factor binding protein 6 IGFBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: HSPCs were purified from discarded umbilical cord blood samples of healthy male or female newborns using the EasySep Human CD34 Positive Selection Kit II following pre-enrichment using the RosetteSep Pre-enrichment cocktail (Stem Cell Technologies) and mononuclear cell isolation on Ficoll-Paque (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: Total CD8+ T cells were isolated from the peripheral blood of 2 healthy donors using EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies). Isolated CD8+T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 beads (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... CD8 T cells were purified from the spleens of early Nrp1 deleted (d28) or late Nrp1 deleted (d34) Nrp1 E8i-CreERT2 R26-mice using EasySep™ Mouse CD8 T Cell Isolation Kits (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Feeder-free H1 hESCs (WiCell) were cultured on Matrigel in TeSR™-E8™ kit for hESC/hiPSC maintenance (StemCell Tech; #05990). hESCs were used to generate COs using media included in the STEMdiff Cerebral Organoid Kit (StemCell Tech ...
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bioRxiv - Bioengineering 2019Quote: ... MSCs were isolated from bone following commercially available defined protocols of MesenCult™ Proliferation Kit with MesenPure™ (#05512 Stemcell Technologies, CA). The MSCs were cultured in one T25 flask per mouse and allowed to expand for two weeks prior to collection (zero passage) ...
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bioRxiv - Immunology 2021Quote: ... a second bead-based purification step was included according to the manufacturer’s instructions (EasySepTM Human Isolation Kit from STEMCell Technologies, Cologne, Germany). While density centrifugation led to 90% pure PMN ...
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bioRxiv - Immunology 2021Quote: NK cells were purified from uninfected spleens of WT and CypD-/- mice using the EasySep Mouse NK Cell Isolation Kit (Stem Cell Technologies) according to the supplier’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: ... the sample was sorted using an APC-conjugated antibody to NOTCH1 (eBiosciences #17-5765-80) followed by APC magnetic selection using the EasySep kit (Stem Cell Tech) as per instructions and plated in PneumaCult medium with a SMAD inhibitor ...
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bioRxiv - Immunology 2021Quote: ... C57BL/6.OT-I.Ubiquitin-GFP CD8+ T cells were enriched from single-cell suspensions of lymph nodes and spleens using mouse CD8-negative selection kits (Stem Cell Technologies). Enriched CD8 T cells (5×106 ...
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bioRxiv - Cancer Biology 2020Quote: ... we thawed frozen viable breast cancer pleural effusions or ascites from patients and depleted dead cells with EasySep™ Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies) and CD45+ cells with EasySep™ Human CD45 Depletion Kit II (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... iPSCs were differentiated into hematopoietic progenitor cells (HPCs) over the course of 11 days using the STEMdiff Hematopoietic kit (STEMCELL technologies, 05310). Non-adherent cells were then collected and analyzed ...
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bioRxiv - Microbiology 2021Quote: Cortical organoid cultures were differentiated from the healthy iPSC cell line according to the specification of the cerebral organoid kit from StemCell Technologies (#08570) that relies on an established protocol (54) ...
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bioRxiv - Immunology 2021Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Immunology 2021Quote: ... Neutrophils were isolated from the remaining blood pellet via negative selection using the EasySep Direct Neutrophil Isolation Kit (STEMCELL Technologies, Cat# 19666) following manufacturer’s directions ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2021Quote: CD138+ myeloma cells were isolated from patient peripheral blood using a magnetic bead-based approach (EasySep Human CD138 Positive Selection Kit, StemCell Technologies 17877). The CD138+-enriched sample was titrated into 2.5e7 ...
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bioRxiv - Developmental Biology 2022Quote: ... All tissues were subject to removal of red blood cells (RBCs) using immunomagnetic negative selection with the EasySep RBC Depletion kit (STEMCELL Technologies, 18170). 12 w samples were further subjected to EasySep™ Human EpCAM Positive Selection Kit II (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... CAR T cells were positively selected for truncated CD19 using the EasySep™ CD19 Positive Enrichment Kit I or II (StemCell Technologies) (for PSCA-CAR T cells ...
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bioRxiv - Cancer Biology 2022Quote: ... mobilized peripheral blood mononuclear cells (PBMCs) and cord blood) using magnetic beads according to manufacturer’s protocol (EasySepTM Human CD3 Positive Selection Kit II, StemCell Technologies, Vancouver, Canada)
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bioRxiv - Cell Biology 2022Quote: ... iPSC-CM was generated as previously described (ref) using GiWi method (17) or STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit (STEMCELL Technologies, 05010), then further purified by lactate selection (18) ...
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bioRxiv - Developmental Biology 2021Quote: hESCs were single-cell seeded onto 6-well Matrigel-coated plates at 2*106 cells/well for ectoderm and endoderm conditions and 5*105 cells/well for mesoderm condition and treated with the appropriate differentiation medium using the STEMdiff Trilineage Differentiation Kit (StemCell Technologies #05230) according to manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: Leukopak-derived peripheral blood mononuclear cells (PBMCs) purchased from LifeSouth Community Blood Centers were debulked with RosetteSep CD8+ enrichment kits (Stem Cell Technologies), and then separated into an enriched fraction of PBMC using a Ficoll gradient ...
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... CD138+ primary myeloma cells were purified from fresh bone marrow aspirates of multiple myeloma patients using EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (StemCell Technologies) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: CD4 T cells were isolated from PBMC by negative magnetic selection using the EasySep Human CD4 T Cell Enrichment Kit (StemCell Technology, 19052). Purity was typically >98%.
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bioRxiv - Immunology 2022Quote: ... Naïve human CD4+ T cells were isolated using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse CD8+ T cells were isolated from the mouse spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19853A) according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... Primary human CD8+ were isolated from healthy donor blood with commercially available negative selection kits (RosetteSep Human CD8+ T cell Enrichment Cocktail, STEMCELL technologies, UK) following the manufacturers instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). Lineage-depleted bone marrow was then stained with an antibody cocktail comprised of a lineage cocktail (BioLegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). 2.5-3.0 × 106 lineage-depleted bone marrow was then washed in PBS and then resuspended in 135 μL Buffer T to which 15 μL of Dre mRNA (at 1 μg/μL ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). Subsequently ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were then enriched for B cells by using the EasySep Human Pan-B cell Enrichment Kit (Stemcell Technologies, Vancouver, BC, Canada). PBMCs were incubated with an antibody cocktail containing CD2 ...
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bioRxiv - Immunology 2020Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Immunology 2020Quote: ... Primary CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) and enriched via negative selection with an EasySep Human CD4+ T Cell Isolation Kit (Stemcell Technologies, Canada). Primary CD4+ T cells were cultured at 37°C in RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2019Quote: Neutrophils were isolated from peripheral venous blood using a Percol density gradient as described previously (17) and purified using the EasySep™ Human Neutrophil Enrichment Kit (STEMCELL Technologies) as per the manufacturer’s instructions (18) ...
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bioRxiv - Immunology 2019Quote: ... The sample was resuspended in growth media and B cells were negatively selected using the EasySep Human B cell Isolation Kit according to the manufacturer’s protocol (STEMCELL Technologies, Vancouver, BC). Because our samples contained greater than one million PBMCs ...
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bioRxiv - Immunology 2021Quote: 1 × 105 memory CD4+ T cells freshly purified from PBMC by negative selection and magnetic separetion(EasySep™ Human memory CD4+ T cell enrichment kit, STEMCELL Technologies) were cocultured at 1:1 ratio for 6 days with anti-CD3/CD28–coated beads (Dynabeads ...
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bioRxiv - Immunology 2021Quote: ... Treg were purified from CD4+ memory T cells by magnetic separation (EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit, STEMCELL Technologies) and labelled with CellTrace™ Violet ...
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bioRxiv - Cell Biology 2020Quote: ... mTESR™ 1 medium was replaced with STEMdiff™ Hematopoietic Supplement A medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). On day 3 ...
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bioRxiv - Immunology 2020Quote: ... and CD8+ T cells were negatively isolated using the EasySep™ Mouse CD8+ T cell Isolation Kit (Stem Cell Technologies, Grenoble, France) or MojoSort™ Isolation Kits (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Immunology 2021Quote: ... CD4+ CD62L+ naïve T cells were isolated from 8-12 week-old mice using CD4 negative enrichment kits (Stemcell Technologies, Vancouver, Canada) and CD62L microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... from individual mice were pooled and pre-enriched for CD4 T cells using EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies). Live ...
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bioRxiv - Immunology 2020Quote: ... And pNK cells were isolated from whole blood by negative selection using the EasySep™ Direct Human NK cell Isolation Kit (StemCell Technologies). Following isolation ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: ... Naïve T cells were isolated and purified from spleen and lymph nodes of C57BL/6J mice using EasySep™ Mouse CD4+ T Cell Isolation Kit (Stemcell Technologies).
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bioRxiv - Immunology 2021Quote: ... density-gradient centrifugation and CD34+ cells were isolated using anti-CD34 immunomagnetic beads (positive selection) following the manufacturer’s instructions (EasySepTM Human cord blood CD34+ positive selection kit II, STEMCELL Technologies Cat#17896). CD34+ HSPCs were either freshly used or cryo-preserved until future use ...
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then sorted using magnetic beads conjugated with an anti-CD15 antibody (clone MC-480) (Kit easy step, Stem cell Technology).
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bioRxiv - Immunology 2022Quote: ... Conditionned NSG mice were humanized with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...