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Citations for Stemcell Technologies :
1501 - 1530 of 1530 citations for Human integrin alpha 5 beta 3 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: E14 and RPL39L KO cells were treated for 5h at 37°C and 5% CO2 with 10μM (S)-MG132 (STEMCELL Technologies Catalog #73264) and 5μM Bafilomycin-A1 (STEMCELL Technologies Catalog #74242 ...
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bioRxiv - Bioengineering 2023Quote: ... we washed 80-90% confluent cultures with PBS-and dissociated cultures into a single-cell suspension with 5 min of Accutase (StemCell Technologies Inc.) treatment at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Single cells were centrifuged at 200g for 5 minutes and resuspended in StemFlex supplemented with 10 µM Y-27632 (StemCell Technologies Inc.). We seeded 5.5×106 cells per 5.5 mL of mTeSR+ in each well of a six-well suspension plate ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell culture medium was supplemented with 1:200 Rho-associated protein kinase inhibitor (ROCKi) Y-27632 (final concentration 5 µM, STEMCELL Technologies, 72302) on the first day after passage ...
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bioRxiv - Developmental Biology 2023Quote: ... Four-month-old cortical organoids were randomly selected and transferred to a recording chamber kept at 37L°C using a heating platform and a controller (TC-324C, Warner Instruments) in 5% methyl-cellulose in BrainPhys Imaging Optimized Medium (STEMCELL Technologies, #05796). Imaging was performed using a SP-8X microscope with a multiphoton laser ...
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bioRxiv - Cell Biology 2023Quote: ... The hESCs H1 cell-lines were differentiated using the extended 7-stage protocol (stages 1–4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Developmental Biology 2023Quote: ... and maintained at 37C with 5%CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Tec.) ...
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bioRxiv - Cell Biology 2024Quote: ... on an automated cell-culture platform developed on a Hamilton Microlab STAR Liquid Handling System (Hamilton Company).2,43 Cells were maintained at 37 °C and 5% CO2 in mTeSR1 medium with and without phenol red (STEMCELL Technologies 85850, 05876), supplemented with 1% penicillin– streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and collected in 5 mL of dissociation media consisting of Epicult-B Basal medium containing Mouse Epicult B supplement (Stemcell Technologies, 05610), 5% FBS ...
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bioRxiv - Immunology 2024Quote: ... the cells were pelleted at 200 rcf for 5 min and resuspended in 1 ml of Neurosphere Proliferation Medium consisting of NeuroCult Basal Medium (StemCell Tech 05700) containing 5 mL NeuroCult Proliferation Supplement (StemCell Tech 05701) ...
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bioRxiv - Cell Biology 2024Quote: ... treated monolayers were washed in PBS then incubated in 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... or β-thalassemia patients were cultured for 14-16d at 37°C and 5% CO2 in SFEM II medium (STEMCELL Technologies, Vancouver, Canada) as previously described37,38 ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were pelleted at 350x g for 2 min and transferred onto a 6-well transwell membrane (Stem Cell Technologies, #3450). Pellets were incubated with CHIR99021 (5 µM ...
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bioRxiv - Physiology 2020Quote: ... and maintained at 37 °C with 5% CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver, Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: Primary mouse clonogenic progenitor assays or colony forming units (CFU) assays were performed by plating 5×105 unfractionated nucleated BM cells in methylcellulose M3434 (StemCell Technologies, Vancouver, Canada) in 1 ml duplicates ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
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bioRxiv - Molecular Biology 2023Quote: ... 2.5% and 5% (v/v) of the hydrophilic concentrate BMGLS in PneumaCult™ ExPlus or PneumaCult™ ALI medium (Stemcell Technologies, Vancouver, Canada) supplemented with MycoZAP (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were collected by centrifugation at 1300 rpm for 5 minutes and 500 K cells distributed into each well of an AggreWell™800 plate (24 well) (Stem Cell Technologies) as recommended by the manufacturer.
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bioRxiv - Molecular Biology 2023Quote: ... Medium was replaced every two days and cells were split every 5 to 6 days using Gentle Dissociation reagent (STEMCELL Technologies, Vancouver, Canada). hES cells were routinely tested for Mycoplasma using the Mycoplasma PCR Detection kit (Applied Biological Materials ...
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bioRxiv - Molecular Biology 2023Quote: ... hPSC-derived NPCs were seeded at an initial concentration of 5×105 cells/mL in STEMdiff™ Neural Progenitor Medium (Stem Cell Technologies) and allowed to sit at 37 °C for 48 hrs ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: Mouse Intestinal Organoids were incubated in 24-well cell culture plates at 37 °C under 5% CO2 in IntestiCult Complete Organoid Medium (#06005, Stem Cell Technologies, Canada) containing CHIR-99021 (3 μM final concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... NK cells were stained with CellTrackerTM Green CMFDA (5-chloromethylfluorescein diacetate) (InvitrogenTM C7025) diluted to 10 µM in serum free ImmunoCultTM NK Cell Base Medium (STEMCELL Technologies 100-0712) for 30 min ...
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bioRxiv - Immunology 2024Quote: ... the pooled blood was carefully layered over 5 mL of Ficoll-Paque PREMIUM 1.084 (Cytiva, Uppsala, Sweden) in 15 mL SepMate-50 tubes (STEM Cell Technology, Cambridge, MA). PBMCs were collected from the interphase layer after centrifugation at 1200xg for 30 min.
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bioRxiv - Genomics 2024Quote: ... hESCs were fed every day for mTeSR1 or every 2 days for mTeSR Plus and passaged every 5–6 days using ReLeSR (Stem Cell Technologies 05872).
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bioRxiv - Synthetic Biology 2024Quote: ... HSPCs derived from healthy patients or iPSC-derived HPCs were cultured for 14d at 37°C and 5% CO2 in SFEMII medium (STEMCELL Technologies, Vancouver, Canada) as previously described19,20 ...
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bioRxiv - Developmental Biology 2022Quote: ... Roughly separated tubules were incubated in collagenase type IV in DMEM (5 mL for 1 testis pair, 1 mg/mL, STEMCELL Technologies Inc., Cat# 07909) at 37 °C while rotating ...
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bioRxiv - Developmental Biology 2024Quote: ... and the organoid pellet was resuspended in 5-10 mL pre-cooled basal DMEM/F-12 medium containing 15 mM HEPES (Stem Cell Technologies, cat.# 36254) using a 10 mL pipette pre-wetted with 1% (vol/vol ...
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bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich], and 10 uM Y27632 [72304, StemCell Technologies] in mTeSR1), and centrifuged at 3200 xg for 30 to 90 min ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were kept in a 37°C tissue culture incubator with 5% CO2 and lines were maintained in mTeSR Plus cultured media (Stemcell Technologies Cat# 100-1130). Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL ...