Labshake search
Citations for Stemcell Technologies :
1451 - 1500 of 1530 citations for Human integrin alpha 5 beta 3 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... H9-hESC SOX10::GFP reporter and H20961 iPSC were passaged ∼1:6 every 4-5 days by incubating them in ReLeSR (StemCell technologies) for 1 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Human pluripotent stem cell (hPSC)-derived cerebral organoids were grown at 37°C with 5% CO2 in STEMdiff Cerebral Organoid Kit cell culture media from STEMCELL Technologies Inc ...
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bioRxiv - Developmental Biology 2020Quote: ... The Geltrex was allowed to set for 40 minutes in a 37°C humidified incubator with 5% CO2 before being submerged in IntestiCult media (STEMCELL technologies).
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bioRxiv - Developmental Biology 2022Quote: ... they were specified into a hiPSC-CM/stromal cell mixture using RPMI B27-containing 5 µM IWP-4 (Stem Cell Technologies) followed by another 7 days of RPMI B27+ (RPMI1640 GlutaMAX + 2% B27 supplement with insulin ...
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bioRxiv - Developmental Biology 2019Quote: ... All cell lines were cultured in humidified incubators (37 C and 5% CO2) using TeSR-E8 media (StemCell Technologies, Vancouver, Canada) or STEMdiff Neural Induction Medium (StemCell Technologies ...
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bioRxiv - Bioengineering 2021Quote: 5 wt% GelMAL-peptide and 5 wt% GelMA hydrogels containing 105 LSK/mL were immediately placed in 500 µL SFEM media (#09650, Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Bioengineering 2021Quote: ... at 5×105 LSK/mL and cultured for 2 h at 5% CO2 and 37 °C in 500 µL SFEM media (#09650, Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells (∼20 x 105 cells/cm2) were replated using the medium in which they were last cultured plus 5 μM ROCK inhibitor (#Y-27632, StemCell Technologies) and 5% Gibco™ KnockOut™ Serum Replacement (KSR ...
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bioRxiv - Cell Biology 2020Quote: ... The dissociated glands were spun down at 450 x g for 5 min and resuspended in ammonium chloride solution (STEMCELL Technologies) diluted (4:1 ...
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bioRxiv - Immunology 2020Quote: BMDNs were obtained by crushing long bones the bones in 5% FCS using a mortar and either isolated by negative selection using the EasySep Mouse Neutrophil Enrichment Kit (Stemcell Technologies) or by positive selection using biotinylated antibodies (see supplemental data for more details).
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bioRxiv - Cancer Biology 2021Quote: IL-10 polarized human DCs (3×10^4/well) were incubated with allogeneic CD8+ T cells (1.5 × 10^5/well) in T cell media (Stem Cell Technologies) in 96 well flat bottom tissue culture plates for 4 days ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured in a Cytomat 24 (Thermo Fischer Scientific) at 37°C and 5% CO2 in mTeSR1 medium with and without phenol red (STEMCELL Technologies), supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific).
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bioRxiv - Bioengineering 2020Quote: ... the hiPSCs were collected and seeded at 1.5 × 105 cells/cm2 cell density on glass coverslips with Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor supplemented mTeSR media (5 μM, StemCell Technologies). The culture was maintained with daily media changes until 90% confluency was reached ...
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bioRxiv - Bioengineering 2020Quote: ... and cells were encapsulated in 5 mm diameter hydrogels (20 µL) and cultured for 7 days in 300 µL SFEM media (#09650 Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Cancer Biology 2022Quote: ... Patient samples were cultured in RPMI with 10% FBS and 10% HS-5 conditioned media (ATCC) or SFEMII supplemented with 1x StemSpan CD34+ Expansion Media and 1 µM UM729 (StemCell Technologies).
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bioRxiv - Cell Biology 2022Quote: ... then gently detached with Accutase and reseeded as 2-5×104 cells/cm2 in mTeSR medium containing 10uM Y-27632 (#72304, STEMCELL Technologies) ROCK inhibitor (removed after 48h and exchanged for fresh medium without ROCK inhibitor) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed with Dulbecco’s Modified Eagle Medium (DMEM/F12) and incubated for 70 min (37°C, 5 % CO2) in STEMdiff Neural Rosette Selection Reagent (StemCell Technologies). Rosettes were lifted by gently dispensing DMEM/F12 onto the colonies ...
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bioRxiv - Physiology 2022Quote: ... Proteases were neutralized with medium containing 10% FBS and resulting suspension of cells were incubated in a flask at 37°C 5% CO2 for 2 hours in PneumaCult-Ex Plus medium (Cat. 05040, Stemcell Technologies) with 100 U/mL penicillin and 100 μg/mL streptomycin to remove non-epithelial cells ...
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bioRxiv - Bioengineering 2024Quote: ... HSPCs were thawed and cultured at a concentration of 5×105 cells/ml in the “HSPC medium” containing StemSpan (STEMCELL Technologies) supplemented with penicillin/streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured at 37□°C in the presence of 5% CO2 in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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bioRxiv - Neuroscience 2024Quote: ... Plates were maintained in a 37°C/5% CO2 incubator and cells were fed every four days with neural maturation medium (BrainPhys plus SM1 supplement (Stemcell Technologies) supplemented with 10ng/ml of BDNF ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Neurons were grown at 37°C/5% CO2 and fed via half-media exchange every 3rd day with astrocyte conditioned BrainPhys media (StemCell Technologies) supplemented with 1% FBS ...
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bioRxiv - Genomics 2024Quote: ... in a humidified atmosphere of 5% CO2 at 37°C on low-attachment plates (62) and were dissociated with Accutase (StemCell Technologies) for passaging and plating.
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bioRxiv - Immunology 2024Quote: Female CatchupIVM-red and FVB/N mice aged 8-15 weeks were treated with 5 µg mouse recombinant G-CSF (STEMCELL Technologies) by intraperitoneal injection (i.p. ...
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bioRxiv - Cell Biology 2023Quote: Luminal or luminal progenitor cells were isolated from mouse mammary glands and sorted in 96-well plate filled with 5% FBS EpiCULT full medium (STEMCELL Technologies). 500 luminal cells or 200 luminal progenitors were then transferred to a 48-well plate previously seeded with 10,000 NIH3T3 irradiated feeder cells per well and incubated at 37°C ...
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bioRxiv - Biochemistry 2023Quote: Cells were counted and adjusted to a concentration of 5 x 105 cells/mL with ALDEFLUOR assay (01700; Stem Cell Technologies) buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... transduced mouse bladder urothelial or prostate epithelial organoids were liberated by dissociating Matrigel matrix with Dispase 5 U/ml (STEMCELL Technologies). Organoids were washed with PBS and resuspended in ice cold Matrigel with either 105 EBLM or UGSM and subcutaneously injected into the flanks of six-to eight-week-old male NSG (NOD-SCID-IL2Ry-null ...
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bioRxiv - Neuroscience 2024Quote: ... in a feeder free fashion and passaged every 4∼5 days at a 1:4∼1:6 ratio using ReLeSR (Stemcell technologies) as described previously 88,95 ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated overnight in media supplemented with 5 µM of the Rho-associated protein kinase (ROCK) inhibitor Y-27632 (STEMCELL Technologies).
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bioRxiv - Neuroscience 2024Quote: ... After nucleofection cells were plated onto a 10 cm dish coated with Synthemax (5 μg/cm2) with TeSR-E8 supplemented with CloneR (StemCell Technologies). Cells were cultured to 80% confluency ...
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bioRxiv - Neuroscience 2024Quote: ... iPSC cultures were passaged every 4-5 days when the colonies reached 80% confluency using the dissociation reagent ReLeSR (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: WA09 embryonic stem cells (WiCell, Madison, WI, USA) were expanded and maintained at 37°C/5% CO2 in mTeSR-E8 culture medium (STEMCELL Technologies) on Nunclon cultureware coated with LDEV-free Geltrex (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Basal media containing either a 1:1,000 dilution of remdesivir stock (5 mM final concentration) or the equivalent volume of DMSO vehicle was prepared in PneumaCult-ALI medium (STEMCELL Technologies) and was used to replace media in the appropriate wells of the 24-well plates containing the transwells ...
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bioRxiv - Cancer Biology 2022Quote: ... all the unwanted cells were labelled with magnet conjugated antibody cocktail which is separated using their EasySep magnetic stand leaving CD4 or CD8 cell in suspension using the vendor supplied EasySep Human CD4/CD8 T Cell Iso Kit (Stem Cell Technologies, 17952 for CD4 and 17953 for CD8). This negative selection approach results in isolation of untouched CD8 or CD4 T cells and stored frozen with 10% DMSO (MP Biomedicals ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C for 5 minutes and replated in a ratio of 1:6 or 1:9 on new Matrigel-coated plates in E8 supplemented with 5 μM ROCK inhibitor Y-27632 (Stemcell Technologies, Canada) for 24h ...
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bioRxiv - Genetics 2019Quote: 1×10^5 cells from the RF of xenotransplanted mice were transferred to 1ml of MethoCult H4034 Optimum methylcellulose medium (StemCell Technologies, 04034) and plated onto a 35mm dish for human specific colony formation ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal bovine serum and subsequently resuspended at a concentration of 5×105 cells/mL in 4-5mL of MethoCult H4230 methylcellulose (STEMCELL Technologies, 04230) containing penicillin/streptomycin ...
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bioRxiv - Bioengineering 2021Quote: ... we first patterned the hiPSCs with a mesenchymal protocol as previously described.(Lam, et al., 2014) Cells were treated with 5 µM CHIR99021 (STEMCELL Technologies, 72052) in Roswell Park Memorial Institute Medium 1640 (RPMI 1640 ...
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bioRxiv - Bioengineering 2021Quote: hiPSC-derived endothelial cells (hECs) were differentiated as previously described.[85] hiPSCs were subjected to 5 μM CHIR99021 in STEMdiff APEL 2 Medium (STEMCELL Technologies, 05270) for 24 hours ...
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bioRxiv - Bioengineering 2021Quote: ... we first directed the hiPSCs towards an intermediate mesenchymal fate using a previously described protocol,[84] wherein cells are treated with 5 µM CHIR99021 (STEMCELL Technologies, 72052) in Roswell Park Memorial Institute Medium 1640 (RPMI 1640 ...
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bioRxiv - Neuroscience 2021Quote: ... These cells were cultured in mTeSR™1 medium (mTeSR™1 Basal Medium with mTeSR™1 5× Supplement (Stemcell Technologies) and 1% penicillin-streptomycin (P/S ...
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bioRxiv - Molecular Biology 2022Quote: ... The differentiation was started following 24 h of seeding and proceeded through a 7-stage differentiation protocol (stages 1 – 4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific, Waltham, MA] supplemented with 10% fetal bovine serum [Stemcell Technologies, Vancouver ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed cells were cultured at 37°C and 5%O2 in serum-free HSC media comprised of StemSpan II medium (Stem Cell Technologies) supplemented with CC100 cytokine cocktail (Stem Cell Technologies) ...
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bioRxiv - Immunology 2021Quote: ... Cells were fed for 4-5 days until airlifted and continued to be grown at air-liquid interface (ALI) with PneumaCult ALI medium (StemCell Technologies, #05001) until fully differentiated (4 weeks)
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Then cells were seeded at 70% confluency on dishes pre-coated with Matrigel and cultured in StemMACS iPS-Brew XF + 5 μM Y-27632 (STEMCELL Technologies 72304). On D1 ...
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bioRxiv - Genetics 2024Quote: ... CD34+ HSPCs were cultured at 1×105–5×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies, Vancouver, Canada) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM ...
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bioRxiv - Developmental Biology 2023Quote: ... and maintained at 37C with 5%CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Tec.) ...