Labshake search
Citations for Stemcell Technologies :
101 - 150 of 4682 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... CD4+ T cells were isolated using EasySepTM Direct Human T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions and activated by incubation in the presence of 100 U/ml IL-2 (Sigma Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: T cells were enriched using the Easy Sep Human T cell Isolation Kit (Stemcell Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... T cells were isolated by EasySep™ Human T Cell Isolation Kits (STEMCELL Technologies, 17951). Naïve T cells were isolated by EasySep™ Human Naïve Pan T Cell Isolation Kits (STEMCELL Technologies ...
-
bioRxiv - Immunology 2020Quote: Total or naïve mouse CD4+ T cells were isolated from the spleen or mesenteric LNs by negative selection with the EasySep Mouse CD4+ T cell enrichment kit or the EasySep Mouse naïve CD4+ T cell enrichment kit according to the manufacturer’s protocol (Stemcell Technologies). In certain cases ...
-
bioRxiv - Immunology 2021Quote: ... Primary CD8+ T cells were isolated using EasySep Human CD8+ T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI-1640 (ATCC ...
-
bioRxiv - Immunology 2023Quote: ... Primary human T cells were isolated using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
-
bioRxiv - Immunology 2019Quote: CD4+ T-cells were isolated from splenocytes and lymph node cells of wild type C57Bl/6 mice (6-8weeks old) using a T-cell negative selection kit (Easy Sep CD4+ T-cell enrichment kit, Stem Cell Technologies) to > 90% purity according to the manufactureŕs instructions ...
-
bioRxiv - Immunology 2021Quote: T cells were isolated from naïve spleens with the EasySep T cell isolation kit (STEMCELL Technologies). For each mouse 4×106 naïve T cells were injected intravenously via the tail ...
-
bioRxiv - Immunology 2020Quote: ... bulk CD4+ T cells were isolated using the EasySep CD4+ T cell Enrichment Kit (StemCell Technologies). Isolated CD4+ T cells were maintained in RPMI medium supplemented with 10% fetal bovine serum and 1% penicillin and streptomycin (R10 medium) ...
-
bioRxiv - Immunology 2019Quote: ... Bulk T cells were isolated from PBMCs using Easysep Human T Cell Isolation Kit (STEMCELL Technologies) and activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... T-cells were obtained from PBMCs using the EasySep Human T-cell Isolation Kit (Stemcell Technologies). MLRs were set up in 96 well plates ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated with EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies, 17952).
-
bioRxiv - Genomics 2021Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and T cells isolated using the EasySep Human T Cell Isolation kit (Stemcell Technologies, #17951). Primary human T cells were cultured for up to 20 days in ATCC-modified RPMI (Thermo Fisher ...
-
bioRxiv - Immunology 2022Quote: CD3ε+ T cells were isolated using negative selection Human T cell Isolation Kit (StemCell Technology, #17951). Cells were plated at 1 × 106 cells/ml in T cell growth media (StemCell Technology ...
-
bioRxiv - Bioengineering 2023Quote: ... Primary T cells were isolated from PBMCs using EasySep Human T Cells Isolation Kits (StemCell Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... T cells were isolated from PMBCs using a Nonhuman Primate T-Cell Isolation Kit (STEMCELL Technologies), activated with a nonhuman primate T-Cell Activation/Expansion Kit (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2020Quote: ... were purified via negative selection (EasySep™ Mouse CD4+ T cell Isolation Kit, STEMCELL Technologies, Lot: 18H94653). Purified CD4+ T cells were cultured for 72 hours with interleukin-12 (IL-12 ...
-
bioRxiv - Immunology 2022Quote: ... and CD8+ T-cell Isolation Kit (StemCell Technologies #19851 ...
-
bioRxiv - Cancer Biology 2020Quote: ... CD8+ T-cell isolation kit was from STEM cell technologies. Anti-PD1 (clone RMP1-14 ...
-
bioRxiv - Immunology 2021Quote: 0.25-0.5 million non-parenchymal cells were used with the EasySepTM Mouse T cell Isolation Kit (Stemcell Technologies, Cat.# 19851) for negative selection of T cells ...
-
bioRxiv - Immunology 2021Quote: ... C57BL/6.OT-I.Ubiquitin-GFP CD8+ T cells were enriched from single-cell suspensions of lymph nodes and spleens using mouse CD8-negative selection kits (Stem Cell Technologies). Enriched CD8 T cells (5×106 ...
-
bioRxiv - Cancer Biology 2023Quote: ... the cells that passed through 70-uM cell strainer were purified with EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies Inc). For T-cell proliferation assays ...
-
bioRxiv - Cell Biology 2020Quote: . Primary human CD3+ and CD8+ T cells were isolated from unfractionated PBMCs using the EasySep Human T cell Isolation Kit and Human CD8 T cell isolation kit with RapidSpheres (Stemcell Technologies). Mobilized human primary CD34+ cells from Peripheral Blood (were obtained from Caltag Medsystems ...
-
bioRxiv - Bioengineering 2020Quote: ... CD4pos and CD8pos T cells were isolated by negative selection using EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
-
bioRxiv - Immunology 2022Quote: CD4+ T cells and CD25hi CD4+ T cells were negatively isolated from PBMCs separately by using Easysep human CD4+ T cell isolation kits and EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies), respectively ...
-
bioRxiv - Immunology 2024Quote: ... CD4+ T cells and CD8+ T cells were enriched using the EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
-
bioRxiv - Genomics 2020Quote: ... T cells were purified using the EasySep™ Direct Human T Cell Isolation Kit (StemCell Technology #19661).
-
bioRxiv - Immunology 2019Quote: ... CD4+ T cells were isolated by negative selection (RosetteSep Human CD4+ T cell Enrichment Kit, Stemcell technologies) following the manufacturer’s procedure ...
-
bioRxiv - Microbiology 2019Quote: ... and CD4+ T cells were isolated using EasySep Human CD4+ T cell isolation kit (StemCell Technologies #17952). Cells were resuspended to 2.5 × 106 cells/mL in RPMI complete media supplemented with 10% FBS ...
-
bioRxiv - Microbiology 2023Quote: ... Splenic T cells were isolated for transfer using a T cell negative selection kit (STEMCELL Technologies 19851) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were isolated from PBMCs using EasySep™ Human T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... naïve CD4 T cells were purified using the naïve human CD4+ T cell isolation kit (StemCell Technology) and stimulated with immobilized anti-human CD3 (5 μg/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... T cells were negatively purified with EasySepTM beads (human T-cell isolation kit, STEMCELL Technologies, Vancouver, Canada). T cells were cultured in Advanced RPMI 1640 enriched with FBS (10%) ...
-
bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated using the EasySep Human CD4+ T cell Isolation Kit (Stemcell Technologies, 17952) and subsequently activated using the T Cell Activation/Expansion Kit (Miltenyi Biotec ...
-
bioRxiv - Immunology 2023Quote: ... and T cells were purified using the EasySep™ Human T Cell Isolation Kit (StemCell Technologies, 17951). Once purified ...
-
bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were isolated using EasySep Human Naïve CD4+ T Cell Isolation Kit (Stemcell technologies), according to manufacturer’s indications.
-
bioRxiv - Bioengineering 2024Quote: ... T cells were isolated using the EasySepTM Human T cell isolation kit (StemCell Tech., Saint-Egrève, France) and cultured in complete X-Vivo15 medium ...
-
bioRxiv - Immunology 2022Quote: ... and CD8+ T cells were negative purified from cell suspension with untouched CD8+ T cell isolation kit (Stemcell Technologies). For the isolation of donor CD45.2+ OT-I T cells from C57BL/6 CD45.1+ recipient animals ...
-
bioRxiv - Immunology 2024Quote: ... CD4 T cells were isolated from the spleens of young (2- to 3-month-old) mice spleen using a magnetic microbeads negative selection kit (EasySep Mouse CD4 T Cell Isolation Kit; STEMCELL Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: Memory and effector CD4 T cells were purified from total splenocytes isolated from immunized mice using EasySepTM Mouse Memory CD4+ T Cell Isolation Kit (StemCell Technologies) as per manufacturer instructions ...
-
bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were either harvested to assess levels of exhaustion marker expression by flow cytometry or DMSO-treated cells were purified using the mouse CD8 T cell isolation kit (StemCell Technologies). Enriched mouse CD8+ T cells were then treated with either DMSO alone or soquelitinib at concentrations ranging from 0 to 10 μM with additional two rounds of stimulation with the OVA peptide ...
-
bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated from pooled splenic suspensions by magnetic negative selection using EasySep Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s instruction ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated from the pooled cell suspensions using an EasySep(tm) Mouse CD4+ T Cell Isolation Kit from Stemcell Technologies (Vancouver, Canada) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: Primary human T cells were isolated from healthy donors using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies). Buffy coats were purchased from Stanford Blood Center and processed according to the manufacturer’s protocol using Lymphoprep density gradient medium and SepMate-50 tubes ...
-
bioRxiv - Cancer Biology 2021Quote: ... CD8+ T cells were isolated from freshly-thawed PBMCs using the human CD8+ T cell isolation kit (Stem Cell Technologies). Anti-ILT3 52B8 (1µg/mL ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T cells were purified from non-adherent cells using Human Resting CD4+ T cell Isolation Kit (STEMCELL Technologies, 17962). A specific reagent to isolate resting T cells was selected to avoid the presence of pre-activated CD4+ T cells in sequencing samples ...
-
bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were purified by negative selection using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) and SepMate-50 tubes ...
-
bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were harvested from SPLNs and MLNs using a CD4+ T cell isolation kit (STEMCELL technologies). Cells (2 × 105 cells/well ...