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Citations for Stemcell Technologies :
451 - 500 of 4682 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... CD3+ T cells were isolated by RosetteSep (STEMCELL Technologies).
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bioRxiv - Immunology 2021Quote: ... naïve CD4+ T cells were enriched (StemCell Technologies; 19555) from healthy donor human PBMCs and cultured in 96-well plates ...
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bioRxiv - Microbiology 2022Quote: ... CD4+ T cells were negatively enriched (STEMCELL Technologies, #19052), pelleted ...
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bioRxiv - Cancer Biology 2023Quote: ... and CD28 (ImmunoCult human T cell activator, StemCell Technologies) in total volume of 200 μl complete RPMI medium in a 96-well U bottom plate in the presence of either DMSO or soquelitinib for 48 hours ...
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bioRxiv - Immunology 2023Quote: ... 2 million CD8 T cells (Stemcell Technologies, Cambridge, MA) from three donors were cultured for 48 hours in AIM V Medium (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Primary T-cells were purchased from StemCell Technologies (#70024). Cells were cultured in X-Vivo 15 media supplemented with 5% human serum and 10 ng/mL of IL-2 ...
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bioRxiv - Immunology 2024Quote: ... Human CD8+ T cells were purchased from StemCell Technologies. Viability was assessed via DAPI staining ...
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bioRxiv - Biochemistry 2022Quote: ... Total T-cells were isolated by negative selection (Stem Cell Technologies #17951) and activated with CD3/CD28 Dynabeads (Life Technologies 11132D ...
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bioRxiv - Bioengineering 2023Quote: ... and ImmunoCult Human CD3/CD28 T Cell Activator (Stem Cell Technologies, 10971) was added at 5 μL/mL to a total volume to 200 μL ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells at 106 cells/mL were stimulated with 25 μL/mL ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL Technologies, Vancouver, Canada, catalogue #10991). At day 3 post-stimulation ...
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bioRxiv - Immunology 2019Quote: ... B cells were purified using the EasySep negative selection mouse B cell enrichment kit (Stem Cell Technologies). B cell purity was assessed by flow cytometry using anti CD19 or B220 antibodies (we routinely observed over 98% purity) ...
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bioRxiv - Immunology 2022Quote: Primary B cells were purified using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies) and stimulated with anti-IgM (20µg/mL ...
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bioRxiv - Immunology 2022Quote: ... splenic NK cells were isolated using the EasySep mouse NK cell isolation kit (Stemcell Technologies) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2019Quote: ... and Mononuclear cells were incubated with 1 ml of erythrocytes and the RossetteSep™ CD8+T cell enrichment cocktail to obtain Total CD8+T cells (15063; StemCell Technologies, Canada). The memory cells were eliminated using magnetic beads (8803 ...
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bioRxiv - Immunology 2023Quote: ... Primary CD8+ and CD4+ T cells were isolated by negative selection through either RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) or RosetteSepTM Human CD4+ T Cell Enrichment Cocktail (StemCell technologies ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were immediately processed to isolate CD4+ T cells by negative selection using the EasySep Human CD4+ T Cell Isolation Cocktail (StemCell Technologies, Cat. #17952) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... the CAR T cells were recovered by two days of cultivation in ImmunoCult™-XF T Cell Expansion Medium (Stemcell Technologies, Cat.#10981) supplemented with Human Recombinant IL-2 (12.5 ng/mL ...
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bioRxiv - Bioengineering 2021Quote: ... enriched lineage negative cells were further purified to obtain an enriched Lin-Sca-1+ c-kit+ (LSK) population using EasySep™ Mouse Sca1 and cKit Positive Selection kits (#18756 & #18757, Stemcell Technologies, CA). For ROS studies ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... and used directly for total T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from splenocytes of OT-I mice using a magnetic bead isolation kit (Stemcell Technology) and cultured following a previously published protocol (44) ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T cells were isolated from monocyte-depleted PBMCs using EasySepTM Human CD4+ Selection Kit II (StemCell Technologies Ltd.) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and Naïve CD4+ T cell were isolated from splenocytes with commercial kits (STEMCELL Technologies, Cat No. 19852, 19853, 19765) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... naïve CD45.1/CD45.2+ P14+ CD8+ T cells were purified from spleens using the StemCell CD8+ isolation kit (STEMCELL Technologies) and 500 were adoptively transferred into CD45.1+ mice ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells from Thy1.1+ P14 mice (PBMCs) were enriched using a CD8 MACS-negative selection kit (STEMCELL Technologies). ∼35,000 P14 CD8+ T cells were transferred intravenously into naïve Thy1.2+ C57BL/6 recipient mice ...
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bioRxiv - Cell Biology 2022Quote: ... cryopreserved CD4+ human cord blood T cells were purchased from Stem Cell Technologies. T cells were isolated from mixed T and B cell cultures using nylon wool separation96 ...
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bioRxiv - Genomics 2021Quote: ... 2×107 T cells were aliquoted into CryoStor cryopreservation media (Stem Cell technologies) for long-term storage.
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bioRxiv - Immunology 2023Quote: ... Isolated T cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stem Cell Technologies).
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bioRxiv - Bioengineering 2023Quote: ... were cultured in ImmunoCult XF T Cell Expansion media (Stem Cell Technologies, 10981) at 37 °C with 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... were cultured in ImmunoCult-XF T Cell Expansion media (Stem Cell Technologies, 10981) at 37 °C with 5% CO2 ...
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bioRxiv - Molecular Biology 2019Quote: Human primary CD8+ T cells were purchased from STEMCELL Technologies Inc ...
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bioRxiv - Immunology 2021Quote: ... (2) ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies) was supplemented with 1X Penicillin Streptomycin solution (ICM) ...
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bioRxiv - Microbiology 2020Quote: ... Immunocult human CD3/CD28 T cell activator (STEMCELL Technologies, USA) was used to stimulate the PBMCs as a positive control for immunological activation.
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bioRxiv - Bioengineering 2021Quote: ... Primary human peripheral blood CD8+ T cells (Stemcell Technologies - #70027) were expanded using fresh complete ImmunoCult™-XF T Cell Expansion Medium (Stemcell Technologies - #10981 ...
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bioRxiv - Cancer Biology 2022Quote: ... Human T cells from anonymous donors sourced by STEMCELL Technologies were isolated by negative selection for CD3+ populations as per manufacturer’s instruction (EasySep ...
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bioRxiv - Immunology 2023Quote: ... or ImmunoCult Human CD3/CD28 T cell activator (Stemcell Technologies) was added at a dilution of 1:500.
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bioRxiv - Molecular Biology 2024Quote: ... Pan-T cells were obtained from StemCell Technologies (Catalog: 70024).
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bioRxiv - Cell Biology 2019Quote: ... Hematopoietic stem cells were enriched using the EasySep Mouse Hematopoietic Progenitor Cell Enrichment kit (Stemcell Technologies) and 1*106 HSCs were administered via tail vein injection to recipient mice ...
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bioRxiv - Immunology 2022Quote: NK cells from spleen were enriched by the EasySep Mouse NK cell isolation kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... All CD19+ B cells were isolated (STEMCELL Technologies EasySep Mouse B Cell Isolation kit, STEMCELL Technologies) and mRNA was extracted and purified (PureLink RNA Mini Kit ...
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bioRxiv - Immunology 2023Quote: ... Dendritic cells were isolated with the EasySepTM Mouse Pan-DC Enrichment Kit II (Stem Cell Technologies). T Cells were isolated with the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Immunology 2020Quote: CD4+ and CD8+ T cell subsets were depleted from PBMC using magnetic bead-based CD4 and CD8 negative selection kits (Stem Cell Technologies, Vancouver, Canada). The cell separations were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
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bioRxiv - Immunology 2020Quote: ... Each antibody clone and lot was titrated to optimal staining concentrations using primary murine samples with all appropriate positive and negative controls: polyclonal murine CD8 T cells purified by positive selection kit (Stem Cell Technologies, Vancouver, Canada) stimulated with PMA/Ionomycin via eBioscience Cell Stimulation Cocktail (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: An Easy Step Human CD8+ T Cell Isolation Kit was used to isolate CD8+ T cells from a donor PBMC sample via negative selection (Cat #, 17913, Stem Cell Technologies, Vancouver, Canada).