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Citations for Stemcell Technologies :
1401 - 1450 of 4374 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were plated at concentrations of 1,000 cells per plate in duplicate on methylcellulose medium (MethoCult GF M3434, STEMCELL Technologies). Colonies were scored manually after 8-10 days ...
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bioRxiv - Immunology 2022Quote: ... Intestinal CD4 T cells were isolated by negative selection using the EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies). Thymocyte single cell suspensions were obtained by pressing minced tissue though a 70μm strainer ...
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bioRxiv - Cancer Biology 2022Quote: ... Low-density (1.077g/cc) mono-nuclear cells were obtained from AML BM using Ficoll density gradient centrifugation (Stem cell technologies).
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bioRxiv - Neuroscience 2023Quote: ... We passaged cells when the confluency of culture reached about 85% by using Gentle Cell Dissociation Medium (Stemcell Technologies, #07174) and ROCK inhibitor (Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... total PBMCs or purified human naïve CD4+ T cells were stimulated with the ImmunoCult human T cell activator (StemCell Technologies) in the presence of either DMSO or soquelitinib for 72 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... ∼2,000 CD34+Lin-CD38-CD90loCD45RA-EPCR+ cells were cultured on tissue culture plates containing StemSpan II media (Stem Cell Technologies) supplemented with 50ng/ml recombinant human SCF (Miltenyi Biotec) ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: Naïve CD4+ and total CD8+ T cells were isolated from the healthy donor blood using the EasySep Human Naïve CD4+ T cell or the EasySep Human CD8+ T cell isolation kit(StemCell Technologies). T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Stem cell culture was done using a commercially available media kit (mTeSR Plus; Cat No. 100-0276, STEM CELL Technologies) following the manufacturer’s published protocol [32] ...
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bioRxiv - Cell Biology 2023Quote: ... sorted 250 cells/well were plated in a 6 well plate of MegaCult C Medium plus Lipids (Stem Cell Technologies) with rhEPO (recombinant human erythropoietin) ...
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bioRxiv - Cancer Biology 2023Quote: ... counted and used for isolation of CD34+ cells using EasySep™ human CD34 positive selection kit II (Stem cell technologies) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sorting of cells using the SONY SH800 sorter or obtained with the naïve human CD4+ T cell isolation kit (StemCell Technology). For T helper cell culture ...
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bioRxiv - Cancer Biology 2023Quote: ... CB MNC were enriched for CD34+ cells using the RosetteSepTM Human CB CD34 Pre-Enrichment Cocktail (Stem Cell Technologies, UK) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and cells were counted using a hemocytometer and dead cells were removed with a magnetic bead based dead cell removal kit according to the manufacturer’s instructions (EasySep STEMCELL technologies). Following lung digestion 1/4 of the cell suspension was sorted for CD45+ CD3- CD19- Ly6G- FceRIa- CD5- NK1.1- CD127+ CD90+ ILCs and spiked back into the whole lung single cell solution ...
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bioRxiv - Immunology 2023Quote: ... 2D2 and OT-I cells were isolated respectively with magnetic CD4+ and CD8+ T cell selection beads (EasySep, STEMCELL Technologies).
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bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and incubated with CD45-APC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60018AZ) and CD34-PE (1 µl per 50,000 cells in 100 µL ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cells were routinely cultured on matrigel in mTeSR1 or mTeSR Plus (Stem Cell Technologies cat #85850 or cat #100-0276). Culture and in vitro differentiation of iPS cells into six cell types (motor neurons (MN) ...
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bioRxiv - Cancer Biology 2022Quote: TS543 human glioma cell line was cultured in human NeuroCult™ NS-A Basal Medium (Stem Cell Technologies, cat #05751), supplemented with 10% NeuroCult™ Proliferation Supplement ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from elutriated lymphocytes by negative selection using RosetteSep CD4+ T cell enrichment cocktail (StemCell Technologies) followed by Ficoll/Hypaque (Amersham Biosciences ...
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bioRxiv - Genetics 2022Quote: ... HSPCs were plated at a concentration of 5×102 cells/mL in a methylcellulose-containing medium (GFH4435, Stem Cell Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
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bioRxiv - Immunology 2023Quote: CD11c+ cells were isolated from spleen and bone marrow cultures using EasySep Mouse CD11c Positive Selection kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the H1 ES cell line was differentiated to PP cells using the STEMdiff™ Pancreatic Progenitor Kit (STEMCELL Technologies, 05120) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... T cells are isolated using an EasySep™ Human T Cell Enrichment Kit as described by the manufacturer (Stemcell Technologies). For T cell activation ...
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bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
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bioRxiv - Cell Biology 2023Quote: ... Twenty-four hours later 10/50/500 non irradiated CD34+ cells or 1,500/7,500/15,000 irradiated (3Gy) CD34+ cells per well were plated in 100 μl MyeloCult H5100 medium (STEM CELL technologies). Once a week half medium replacement was done ...
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bioRxiv - Cancer Biology 2024Quote: ... CD138 positive plasma cells were purified from available matching BM aspirates using immunomagnetic bead selection (EasySep™; Stem Cell Technologies). Total RNA from 7 of these patients was extracted from CD138+ cells using the miRNeasy Mini Kit (Qiagen GmbH ...
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bioRxiv - Cancer Biology 2024Quote: Both WT and MT iPS cells were maintained in mTeSRTM1 medium with 1x Supplement media (Stem Cell Technologies, Catalog #85850) and supplemented with 10 μM Y27632 (Stemgent ...
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bioRxiv - Immunology 2024Quote: ... Tregs were seeded at an approximate density of 1.2 million cells/cm2 in ImmunoCult-XF T cell expansion medium (STEMCELL Technologies) supplemented with 50 μM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Murine splenic B cells were isolated by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies, #19854A) as specified by the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Frozen cells were thawed at 37°C and treated using the ErythroClear™ Red Blood Cell Depletion Kit (STEMCELL Technologies). Cells were constructed into barcoded single-cell cDNA libraries and sequenced as described above.
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood B cells were isolated via negative selection using RosetteSep immunodensity-based cell separation (Stemcell Technologies, Vancouver, BC, Canada). The purity of CLL/ B cells was analyzed by flow cytometry and revealed that ≥ 90% cells co-expressed CD5/CD19.
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bioRxiv - Cancer Biology 2024Quote: Human neural progenitor cells (hNPCs) were generated by a single-cell monolayer protocol using STEMDiff Neural Induction Medium (StemCell Technologies) as described previously 19 ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cryopreserved in Cryostor 10 (STEMCELL Technologies). PREDICT96-ALI plates were prepared as previously described13 ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s protocol (Stem Cell Technologies). In brief ...
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bioRxiv - Genomics 2021Quote: ... containing 100μL StemSpan SFEM media (Stem Cell Technologies). Media was supplemented with penicillin/streptomycin (100ng/mL) ...
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bioRxiv - Genomics 2021Quote: ... with 1X CD34+ Expansion Supplement (Stem Cell Technologies). 20ul of mineral oil (Ibidi ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were cultured in TeSR-E6 (STEMCELL Technologies) lacking TGFβ ligand for 24 hours prior to the experiment ...
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bioRxiv - Developmental Biology 2021Quote: ... Gentle Cell Dissociation Reagent and ReLeSR (STEMCELL Technologies) were used for clump passaging ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... 30 ng/ml activin A (Stem Cell Technologies), and 3 µM CHIR99021 (Cayman Chemical ...
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bioRxiv - Developmental Biology 2020Quote: ... and 5 mM Y27632 (Stem Cell Technologies 72304). The medium was changed every two days ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.5 mM A83-01 (Stem Cell Tech. 72024), 1 mM SB431542 (Tocris 1614) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged with ReLeSR™ (StemCell Technologies) and plated in media containing 10 μM Rho-kinase inhibitor Y-27632 (StemCell Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with CC100 cytokine cocktail (Stem Cell Technologies), 100ng/ml TPO (Peprotech ...
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bioRxiv - Molecular Biology 2020Quote: ... using SepMate-50 tubes (Stem Cell Technologies 85450), and centrifuged for 15 min at 1200 x g ...
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bioRxiv - Genomics 2020Quote: ... detached using Accutase (Stem Cell Technologies, Vancouver, Canada), quenched with full cold media ...
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bioRxiv - Developmental Biology 2020Quote: ... and maintained in mTESR1 media (Stem Cell Technologies) at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... containing MyeloCult H5100 (Stem Cell Technologies, cat#5150) supplemented with 1 μM hydrocortisone ...
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bioRxiv - Immunology 2020Quote: ... 8 weeks old males (Stem Cell Technologies #19765), according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: ... and 5 μM Xav-939 (Stem Cell Technologies). On day 5 ...