Labshake search
Citations for Stemcell Technologies :
1301 - 1350 of 4374 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... The collected cells were resuspended in ENR medium (advanced DMEM/F-12 with 15 mM HEPES (STEM CELL Technologies) supplemented with 100 μg/ml Penicillin-Streptomycin ...
-
bioRxiv - Developmental Biology 2022Quote: H9 hESCs were differentiated into early neural progenitor cells (eNPCs) with STEMdiffTM Neural Induction Medium (Stem Cell Technologies, #05835) following the manufacturer’s instructions for the monolayer protocol variant ...
-
bioRxiv - Molecular Biology 2022Quote: Myeloid cells were purified from PBMCs using Easy Sep Human Myeloid positive selection kit (Stem cell technology; Cat# 18653). The kit positively selects myeloid cells positive for CD33 and CD66b ...
-
bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were enriched using the EasySep Human CD4+ T Cell Enrichment Kit (STEMCELL Technologies, Cat. No. 19052) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: Differentiation of iPSCs to hematopoietic progenitor cells (HPCs) was done using Stem Cell Technologies STEMdiff Hematopoietic Kit (StemCell Technologies). Upon reaching 80 % confluency ...
-
bioRxiv - Neuroscience 2022Quote: ... NPCs were plated at 50,000 cells/cm2 and passaged at 90% confluency by AccutaseTM (Stem Cell Tech cat#07920) dissociation as necessary ...
-
bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
-
bioRxiv - Genetics 2023Quote: ... cells were plated into stem cell media without penicillin-streptomycin supplemented with CloneR2 (STEMCELL Technologies; cat. no. 100-0691) and 10 μM ROCK inhibitor ...
-
bioRxiv - Neuroscience 2023Quote: ... at a density of 30 x 104 cells/well and cultured in NeuroCult neuronal plating medium (Stem Cell Technologies) supplemented with B-27 (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from naïve splenocytes using the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies) and plated at 3x105 cells per well in a flat bottom 96 well plate ...
-
bioRxiv - Cancer Biology 2023Quote: ... 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies, Cologne, Germany) with 50 IE/ml IL-2 ...
-
bioRxiv - Neuroscience 2023Quote: ... The remaining pellet was gently resuspended in enteric glial cell media supplemented with 1x CloneR (Stem Cell Technologies, 5889) and 50ug/mL primocin (Invivogen ...
-
bioRxiv - Immunology 2023Quote: NK Cells were enriched from freshly isolated PBMC using the EasySep™ Human NK Cell Isolation Kit (STEMCELL Technologies). NK cells were then counted and resuspended at 1M/mL in RPMI-1640 supplemented with 10% fetal bovine serum ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged twice a week after reaching approximately 80% confluency using PBS/EDTA or ReLeSRTM (Stem Cell Technologies) as a dissociation reagent ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended in 4 ml Red Blood Cell Lysis buffer (Ammonium Chloride solution, Stemcell Technology, #07800) on ice for 2 min and washed with 6 mL DME/F-12 medium at 450 g for 10 min ...
-
bioRxiv - Bioengineering 2023Quote: ... Human pan T cells were purified from frozen peripheral blood mononuclear cells (PBMCs, STEMCELL Technologies, Cat #70025.2. Lot 201671701C) using a negative selection human Pan T Cell Isolation Kit (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2022Quote: ... were seeded (70,000 cells each) in medium for long-term culture of human cells (catalogue no. 05150; STEMCELL Technologies) supplemented with hydrocortisone in collagen-coated plates ...
-
bioRxiv - Immunology 2023Quote: ... B cells were extracted by negative selection with the EasySep™ Human B Cell Isolation Kit (StemCell Technologies, #17954). EBV-containing supernatants from B95-8 cells were added to purified B cells supported by 30 µg mL-1 holo-transferrin and 2.5 µg mL-1 CpG (ODN2006) ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were cultured in the presence of TGFβ and BMP inhibitors (LDN193189, #72142, Stem Cell Technologies; SB431542, #1614, Tocris) to trigger cortical precursors for cortical neuron differentiation ...
-
bioRxiv - Immunology 2023Quote: Transduced T cells were depleted of CD56 cells using the EasySep Human CD56 positive selection kit (Stemcell Technologies; 17855) and rested for 24 hours ...
-
bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 300g for 5 mins and RBC lysed using Ammonium Chloride Lysis Buffer (Stem Cell Technologies) as per manufacturer protocol ...
-
bioRxiv - Biophysics 2023Quote: ... Cells are cultivated in Epicult basal medium with Supplement C (Epicult-C human media kit, Stem Cell Technologies, Vancouver), 5% FCS ...
-
bioRxiv - Immunology 2023Quote: ... CVF was enriched for CD45+ cells using the EasySep™ Release Human CD45 Positive Selection Kit (Stem Cell Technologies) according to manufacturer protocol ...
-
bioRxiv - Developmental Biology 2023Quote: ... For stem cell maintenance N2B27 medium was supplemented with 3 μM CHIR99021 (Chir) (Catalog no. 72054, Stem Cell Technologies), 1 μM PD0305901 (Catalog no ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were cultured in collagen-I gels in the ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies, Cat# 10981) with the addition of Human Recombinant Interleukin 2 (STEMCELL Technologies ...
-
bioRxiv - Developmental Biology 2023Quote: ... The OSCs were produced in multiple batches and cryopreserved in vials of 120,000 to 150,000 live cells and stored in liquid nitrogen in CryoStor CS10 Cell Freezing Medium (StemCell Technologies). For the purposes of this study ...
-
bioRxiv - Developmental Biology 2024Quote: ... iPSCs were washed 1x in PBS and dissociated into single-cell suspensions using Gentle Cell Dissociation Reagent (Stemcell Technologies) for 10 min at 37° C and seeded on to Geltrex-coated 12-well plates at a density of 700,000 cells in neural induction medium + 10 uM Y-27632 (Stemcell Technologies ...
-
bioRxiv - Immunology 2024Quote: ... Effector CD4+ T cells were isolated by magnetic enrichment using EasySep mouse CD4+ T cell Isolation Kit (STEMCELL Technologies) with addition of 10 µL of biotinylated anti-CD62L (BioLegend ...
-
bioRxiv - Genomics 2024Quote: ... mouse NIH-3T3 cells were washed once with PBS and dissociated with Accutase (Stem Cell Technologies, catalog no. 07920). Two million NIH-3T3 and K562 cells were collected and fixed in a 3 % glyoxal solution (40 % glyoxal (Merck ...
-
bioRxiv - Immunology 2024Quote: ... CD3 T cells were isolated by negative selection following manufacturer’s protocol (RosetteSep Human T cell enrichment cocktail, STEMCELL Technologies). Following isolation ...
-
bioRxiv - Immunology 2023Quote: ... T cells were obtained from whole blood using the RosetteSep™ Human T Cell Enrichment Cocktail (StemCell Technologies, 15061), followed by density gradient separation using Ficoll® Paque Plus (Cytiva Life Sciences ...
-
bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19258). All isolated T cells were activated by CD3/CD28 Dynabeads (Thermo Fisher ...
-
bioRxiv - Immunology 2024Quote: ... B cells were isolated from the samples using RosetteSep Human B cell Enrichment Cocktail (Stemcell Technologies, Vancouver, BC, Canada) following manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... CD4+ T cells were activated on-chip by adding ImmunoCult™ Human CD3/CD28 T Cell Activator (StemCell Technologies) to the culture media ...
-
bioRxiv - Cancer Biology 2024Quote: ... CD3+ T-cell populations were isolated using EasySep Human T Cell Isolation Kit (StemCell Technologies, Cat# 17951, lOT #1000153615). CD3+ T-cells were thawed ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The OSCs were produced in multiple batches and cryopreserved in vials of 120,000-150,000 live cells and stored in liquid nitrogen in CryoStor CS10 Cell Freezing Medium (StemCell Technologies). The morning of oocyte retrieval ...
-
bioRxiv - Immunology 2024Quote: ... Total CD8+ T cells were isolated by magnetic sorting (EasySep™ Mouse CD8+ T Cell Isolation Kit, StemCell Technologies), followed by tetramer E7 and TRM marker staining ...
-
bioRxiv - Immunology 2023Quote: iPSc cell lines were furnished by the Nantes iPSc platform and were maintained on Matrigel (Stem Cell Technologies, 354277) coated 6-well plates in mTESR1 medium (Stem Cell Technologies) ...
-
bioRxiv - Bioengineering 2024Quote: ... 600000 single stem cells were seeded per well (500 cells/microwell) of AggreWell™400 (STEMCELL Technologies, Vancouver, Canada) plates in aggregation media with 50 μM Y27632 (STEMCELL Technologies) ...
-
bioRxiv - Immunology 2023Quote: ... dendritic cells (DCs) were enriched by magnetic isolation using the EasySepTM Mouse Pan-DC Enrichment Kit (Stem Cell Technologies). DCs were plated into 96-well U-bottom plates (100 × 106 cells/well) ...
-
bioRxiv - Cancer Biology 2024Quote: 500 transduced MDA-MB-436 cells were mixed with 1 ml of methylcellulose (MethoCult H4034 Optimum, Stem Cell Technologies) in a 6-well cell culture plate and cultured at 37 °C in a 5% CO2 incubator ...
-
bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...
-
bioRxiv - Bioengineering 2021Quote: ... microcapsules carrying HUES-8 cells were immersed in E8 medium for 1 h immediately after encapsulation and then transferred into E6 medium (without FGF-2 and TGF-β1, STEMCELL Technologies) for subsequent cultivation (2000 microcapsules per well) ...
-
bioRxiv - Bioengineering 2021Quote: ... at 5×105 LSK/mL and cultured for 2 h at 5% CO2 and 37 °C in 500 µL SFEM media (#09650, Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
-
Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Cells were plated at a density of 2-3×103/cm2 in pEPSCM medium27 containing the Rho-associated coiled kinase (ROCK) inhibitor Y-27632 (5μM, Stemcell Technologies, #72304). Cells were fed the following day with pEPSCM without Y-27632 then fed daily ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
-
bioRxiv - Physiology 2022Quote: ... Proteases were neutralized with medium containing 10% FBS and resulting suspension of cells were incubated in a flask at 37°C 5% CO2 for 2 hours in PneumaCult-Ex Plus medium (Cat. 05040, Stemcell Technologies) with 100 U/mL penicillin and 100 μg/mL streptomycin to remove non-epithelial cells ...
-
bioRxiv - Neuroscience 2023Quote: ... 550,000 neurons were seeded in 6 well plates coated with 1 mg/mL polyLysine were cultured in conditioned Neurobasal medium supplemented with 2 mM L-glutamine and NeuroCult SM1 Neuronal supplement (STEMCELL Technologies) half renewed every 3 days with 3.4mM AraC ...
-
bioRxiv - Cancer Biology 2023Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
-
bioRxiv - Bioengineering 2023Quote: ... The PBMC pellet was resuspended in PBS supplemented with 2% FBS and 1 mM EDTA and monocyte isolation was performed using the EasySepTM human monocyte isolation kit (Stemcell Technologies) according to manufacturer instructions ...