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Citations for Stemcell Technologies :
1251 - 1300 of 4776 citations for Killer cell immunoglobulin like receptor 2DL2 KIR2DL2 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The cells were resuspended in 1 mL of medium contained 1x Stem Span Cytokine Cocktail CC100 (Stem Cell Technologies, Canada) in 24-well plate ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were used to isolate T cells by CD3-negative selection (EasySep Human T cells Isolation, STEMCELL Technologies, Vancouver, Canada). Tumor-infiltrating lymphocytes (TILs ...
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bioRxiv - Immunology 2021Quote: ... Untouched CD8+ T cells were magnetically isolated using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, Grenoble, France) or MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Naive CD4+ T cells were isolated by negative selection with the EasySep Human Naïve CD4+ T Cell Isolation Kit (STEMCELL Technologies) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Human cord-blood CD34+ cells were isolated by magnetic sorting using EasySep human CD34 positive selection kit (Stem Cell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were plated at concentrations of 1,000 cells per plate in duplicate on methylcellulose medium (MethoCult GF M3434, STEMCELL Technologies). Colonies were scored manually after 8-10 days ...
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bioRxiv - Immunology 2022Quote: ... Intestinal CD4 T cells were isolated by negative selection using the EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies). Thymocyte single cell suspensions were obtained by pressing minced tissue though a 70μm strainer ...
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bioRxiv - Cancer Biology 2022Quote: ... Low-density (1.077g/cc) mono-nuclear cells were obtained from AML BM using Ficoll density gradient centrifugation (Stem cell technologies).
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bioRxiv - Neuroscience 2023Quote: ... We passaged cells when the confluency of culture reached about 85% by using Gentle Cell Dissociation Medium (Stemcell Technologies, #07174) and ROCK inhibitor (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... CD138 positive plasma cells were purified from available matching BM aspirates using immunomagnetic bead selection (EasySep™; Stem Cell Technologies). Total RNA from 7 of these patients was extracted from CD138+ cells using the miRNeasy Mini Kit (Qiagen GmbH ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood B cells were isolated via negative selection using RosetteSep immunodensity-based cell separation (Stemcell Technologies, Vancouver, BC, Canada). The purity of CLL/ B cells was analyzed by flow cytometry and revealed that ≥ 90% cells co-expressed CD5/CD19.
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bioRxiv - Cancer Biology 2024Quote: Human neural progenitor cells (hNPCs) were generated by a single-cell monolayer protocol using STEMDiff Neural Induction Medium (StemCell Technologies) as described previously 19 ...
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bioRxiv - Immunology 2024Quote: ... Murine splenic B cells were isolated by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies, #19854A) as specified by the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Tregs were seeded at an approximate density of 1.2 million cells/cm2 in ImmunoCult-XF T cell expansion medium (STEMCELL Technologies) supplemented with 50 μM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: Both WT and MT iPS cells were maintained in mTeSRTM1 medium with 1x Supplement media (Stem Cell Technologies, Catalog #85850) and supplemented with 10 μM Y27632 (Stemgent ...
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bioRxiv - Neuroscience 2023Quote: ... Stem cell culture was done using a commercially available media kit (mTeSR Plus; Cat No. 100-0276, STEM CELL Technologies) following the manufacturer’s published protocol [32] ...
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bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
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bioRxiv - Cancer Biology 2023Quote: ... CB MNC were enriched for CD34+ cells using the RosetteSepTM Human CB CD34 Pre-Enrichment Cocktail (Stem Cell Technologies, UK) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and cells were counted using a hemocytometer and dead cells were removed with a magnetic bead based dead cell removal kit according to the manufacturer’s instructions (EasySep STEMCELL technologies). Following lung digestion 1/4 of the cell suspension was sorted for CD45+ CD3- CD19- Ly6G- FceRIa- CD5- NK1.1- CD127+ CD90+ ILCs and spiked back into the whole lung single cell solution ...
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bioRxiv - Cancer Biology 2023Quote: ... counted and used for isolation of CD34+ cells using EasySep™ human CD34 positive selection kit II (Stem cell technologies) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sorting of cells using the SONY SH800 sorter or obtained with the naïve human CD4+ T cell isolation kit (StemCell Technology). For T helper cell culture ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: Naïve CD4+ and total CD8+ T cells were isolated from the healthy donor blood using the EasySep Human Naïve CD4+ T cell or the EasySep Human CD8+ T cell isolation kit(StemCell Technologies). T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and incubated with CD45-APC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60018AZ) and CD34-PE (1 µl per 50,000 cells in 100 µL ...
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bioRxiv - Cancer Biology 2022Quote: TS543 human glioma cell line was cultured in human NeuroCult™ NS-A Basal Medium (Stem Cell Technologies, cat #05751), supplemented with 10% NeuroCult™ Proliferation Supplement ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from elutriated lymphocytes by negative selection using RosetteSep CD4+ T cell enrichment cocktail (StemCell Technologies) followed by Ficoll/Hypaque (Amersham Biosciences ...
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bioRxiv - Genetics 2022Quote: ... HSPCs were plated at a concentration of 5×102 cells/mL in a methylcellulose-containing medium (GFH4435, Stem Cell Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
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bioRxiv - Immunology 2023Quote: CD11c+ cells were isolated from spleen and bone marrow cultures using EasySep Mouse CD11c Positive Selection kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... total PBMCs or purified human naïve CD4+ T cells were stimulated with the ImmunoCult human T cell activator (StemCell Technologies) in the presence of either DMSO or soquelitinib for 72 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... ∼2,000 CD34+Lin-CD38-CD90loCD45RA-EPCR+ cells were cultured on tissue culture plates containing StemSpan II media (Stem Cell Technologies) supplemented with 50ng/ml recombinant human SCF (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... sorted 250 cells/well were plated in a 6 well plate of MegaCult C Medium plus Lipids (Stem Cell Technologies) with rhEPO (recombinant human erythropoietin) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cells were routinely cultured on matrigel in mTeSR1 or mTeSR Plus (Stem Cell Technologies cat #85850 or cat #100-0276). Culture and in vitro differentiation of iPS cells into six cell types (motor neurons (MN) ...
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bioRxiv - Developmental Biology 2023Quote: ... the H1 ES cell line was differentiated to PP cells using the STEMdiff™ Pancreatic Progenitor Kit (STEMCELL Technologies, 05120) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... T cells are isolated using an EasySep™ Human T Cell Enrichment Kit as described by the manufacturer (Stemcell Technologies). For T cell activation ...
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bioRxiv - Cell Biology 2023Quote: ... Twenty-four hours later 10/50/500 non irradiated CD34+ cells or 1,500/7,500/15,000 irradiated (3Gy) CD34+ cells per well were plated in 100 μl MyeloCult H5100 medium (STEM CELL technologies). Once a week half medium replacement was done ...
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bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
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bioRxiv - Immunology 2024Quote: ... spleens were mechanically dissociated by a syringe plunger between two pieces of mesh to create a single cell suspension followed by red blood cell lysis by incubation in 0.8% ammonium chloride solution (STEMCELL Technologies) and subsequent staining ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were enriched from total splenocytes using the EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies) (routinely >90% purity).
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bioRxiv - Cell Biology 2024Quote: Single cell suspensions of BMMNCs were first lineage depleted using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies). Lineage depleted cells were then stained with ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) from de-identified healthy donors were purified from leukapheresis products purchased from Stem Cell Technologies using EasySep Direct Human PBMC Isolation Kits (Stem Cell Technologies) ...
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bioRxiv - Immunology 2024Quote: ... Cells were harvested at 72 hours and Annexin V+ dead/dying cells were depleted using immunomagnetic negative selection (STEMCELL technologies). Isotopomeric Spectral Analysis (ISA ...
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bioRxiv - Immunology 2024Quote: ... The samples were enriched for CD4 T cells using the EasySep™ mouse CD4+ T Cell isolation kit (Stemcell technologies) and the dead cells were removed using EasySep™ Dead Cell Removal (Annexin V ...
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bioRxiv - Physiology 2024Quote: ... and the filtered cells were seeded into cell culture dishes at different densities for colony count and at 50,000 cell/cm2 for cell expansion and differentiation in Human MesenCult proliferation Medium (StemCell Technologies).
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bioRxiv - Microbiology 2024Quote: ... Total CD4 T cells were isolated from PBMC using EasySep™ Human CD4+ T Cell Isolation Kit (#17952, StemCell Technologies) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were purified using the negative selection kits RosetteSep™ Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies). To obtain primary CD45RA+CD8+ naïve T cells or CD45RO+CD8+ memory T cells ...
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bioRxiv - Microbiology 2024Quote: ... and total CD4+ T-cells were obtained via negative immunomagnetic selection (EasySep Human CD4+ T-cell Enrichment Kit, Stemcell Technologies), with cell count ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were isolated from peripheral blood of healthy donors with Human T Cell Enrichment Kit (Stem Cell Technology #19051). Human T cells were cultured in RPMI-1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... 1x108 cells/ml were incubated with 20μl/ml EasySepTM mouse Hematopoietic Progenitor Cell Isolation Cocktail (15min, 4°C, StemCell technologies), RapidSpheres (50μl/ml ...
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bioRxiv - Immunology 2024Quote: ... Human T cells were then isolated from a diluted buffy coat using RosetteSep Human T Cell Enrichment Cocktail (StemCell Technologies) and SepMate tubes (StemCell Technologies ...