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Citations for Stemcell Technologies :
1501 - 1550 of 4776 citations for Killer cell immunoglobulin like receptor 2DL2 KIR2DL2 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 40 ng/ml BDNF (Stem Cell Technologies, #78005.1) for 7 days ...
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bioRxiv - Developmental Biology 2024Quote: ... and 6 μM CHIR99021 (Stem Cell Technologies 72054) for 24 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were dissociated with Accutase (STEMCELL Technologies, 07920) and plated onto Cultrex-coated 24-well or 6-well plates in mTeSR1 with ROCK inhibitor (Y-27632 ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 1X NeuroCult SM1 (Stem Cell Technologies), 1X N2 supplement (Thermo) ...
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Synchronous 3D patterning of diverse CNS progenitors generates motor neurons of broad axial identitybioRxiv - Neuroscience 2024Quote: ... Cells were cultured in mTeSR plus (STEMCELL Technologies), which was refreshed every 2-3 days ...
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bioRxiv - Neuroscience 2024Quote: ... or a BrainPhys™-based (Stem Cell Technologies) reprogramming medium containing 1mM valproic acid (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... 1x105 cells were seeded onto collagen (STEMCELL Technologies) coated transwell (0.4 µm pores ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mL ReLeSR (Stem Cell Technologies, 100-0483) per well of a 6-well plate was added ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were dissociated with Accutase (STEMCELL Technologies, 07920) and plated onto Cultrex-coated 6-well plates ...
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bioRxiv - Genetics 2024Quote: Cryopreserved human peripheral blood mononuclear cells (STEMCELL Technologies) were thawed and T cells were isolated using EasySep Buffer and the EasySep Human T Cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded in MethoCultTM M3231 (STEMCELL Technologies), supplemented with cytokines (PeproTech) ...
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bioRxiv - Genetics 2024Quote: ... containing 3μM CHIR99021 (Stem Cell Technologies, Cat.#72054), 500μg/mL BSA (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... or 1:20 Alexa488-conjugated anti-TRA-1-60 antibody (7.5 μg/mL final concentration; STEMCELL Technologies). The cells were stained in 100 μL of diluted antibody per 106 cells overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... where randomly grouped and 2 x 106 naïve Ly5.2+CD8+CD44loCD62Lhi OT-I T cells [purified by FACS or using the EasySep™ murine naïve CD8+ T cell isolation kit (Stemcell Technologies)] were injected intravenously ...
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bioRxiv - Cell Biology 2020Quote: . Primary human CD3+ and CD8+ T cells were isolated from unfractionated PBMCs using the EasySep Human T cell Isolation Kit and Human CD8 T cell isolation kit with RapidSpheres (Stemcell Technologies). Mobilized human primary CD34+ cells from Peripheral Blood (were obtained from Caltag Medsystems ...
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bioRxiv - Cell Biology 2020Quote: ... The buffy coat was first separated by Ficoll-Paque Plus solution and the NK cells were then purified from the Peripheral Blood Mononuclear Cells (PBMCs) by negative selection using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were manually counted on a hemocytometer using Trypan blue and plated in methylcellulose media (M3434 or M3334, Stem Cell Technologies) at a density of 5×105 live cells/ml (in M3434 ...
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bioRxiv - Developmental Biology 2020Quote: ... YS were digested with collagenase I to dissociate the cells into single cell suspension and plated in methylcellulose medium (media M34334, StemCell Technologies) containing cytokines (SCF ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies) or StemMACS HSC Expansion Media (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were initially dissociated into single cell suspensions by accutase treatment (Stemcell Technologies, 07920, 30-60 min, 37 °C, 5% CO2) and then fixed and permeabilized using fixation/permeabilization buffers (Foxp3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Immunology 2022Quote: ... NK cells were isolated from buffy coats from healthy donors using the RosetteSep NK cell enrichment kit (STEMCELL Technologies, MA, USA) and stimulated with rhIL-15 (1ng/ml ...
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bioRxiv - Immunology 2021Quote: Bone marrow colony forming assay was performed by plating 104 or 5×104 cells on methylcellulose (MethoCult M3434, Stem Cell Technologies). GEMM (Granulocyte/Erythrocyte/Macrophage/Megakaryocyte) ...
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bioRxiv - Physiology 2020Quote: ... Single-cell suspensions were plated at a density of 1.2 × 105 cells/cm2 and cultured overnight in mTeSR medium supplemented with 10 μM Y-27632 dihydrochloride (Stem Cell Technologies). Once the monolayer reached approximately 80% confluence (usually the following day) ...
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bioRxiv - Genomics 2020Quote: ... Lymph nodes were harvested from a 6-week-old GFP OT-1 mouse and CD8 T cells were isolated using an EasySep mouse CD8 T cell negative selection kit (StemCell Technologies). 14 days post tumor injection ...
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bioRxiv - Immunology 2021Quote: ... Huh7 and HepG2 and autologous CD8+ T cells (pre-activated with ImmunoCult™ Human CD3/CD28 T Cell Activator, Stemcell Technologies, Canada according to the manufacturer’s instruction) ...
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bioRxiv - Bioengineering 2021Quote: ... A 3x103 cell fraction of non-transduced and transduced Lin-cells were transferred into 3 ml methylcellulose-based medium with mouse cytokines optimized for hematopoietic progenitor cell differentiation (StemCell Technologies) and plated at 1 mL/well for a CFU assay ...
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bioRxiv - Bioengineering 2020Quote: ... Clear supernatant was removed and intestinal pieces were resuspended in 25 ml of Gentle Cell Dissociation Reagent (Stem Cell Technologies, 07174). Tubes were incubated at room temperature on a rocking platform for 20 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies). Isolated T cells were then plated in ImmunoCult-XF T Cell Expansion Medium (StemCell Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... CD4pos and CD8pos T cells were isolated by negative selection using EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies) respectively ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... The CD4+ T cells were purified from 50 × 106 PBMCs using EasySep™Human CD4+ T cell isolation kit (STEMCELL Technologies). Primary CD4+ T cells were cultured at 37°C in a 5 % (v/v ...
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bioRxiv - Immunology 2021Quote: Naïve IgM+IgD+B cells were purified by negative selection using the EasySepTM human naive B cell enrichment kit (19254; StemCell Technologies) from healthy subject PBMCs ...
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bioRxiv - Microbiology 2021Quote: ... buffy coats (from anonymous healthy blood donors from Mississippi Blood Center) were separated by Ficoll and CD4+ T-cells purified using RosetteSep Human CD4+ T-cell enrichment kit (STEMCELL Technologies). CD4+ T-cells cells were maintained in Roswell Park Memorial Institute 1640 medium (RPMI ...
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bioRxiv - Cancer Biology 2021Quote: Human peripheral blood NK cells were isolated from blood collars using a RosetteSep™ human NK cell enrichment cocktail (STEMCELL Technologies). NK cells were then co-cultured for 4 h with confluent monolayers of SH-SY5Y parental ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were spun at 350xg for 10min and the red blood cells were lysed with 5ml Ammonium chloride (StemCell Technologies) per 50ml tube for 5min ...
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bioRxiv - Cell Biology 2021Quote: GMPs were sorted as described earlier from WT (C57Bl6/J) and 50K cells were cultured in 0.5mls of StemSpanTMSFEMII (Stem cell technologies) supplemented with 1% L-Glutamine and Penicillin/Streptomycin in addition to mouse recombinant cytokines ...
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bioRxiv - Bioengineering 2020Quote: ... Cryopreserved PBMCs were quickly thawed and resuspended at a concentration of 4×106 cells/ml in Eagle's minimal essential medium (EMEM; Stem Cell Technologies; supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... media was removed from HNAE cells apical side (airlift) and provided with Pneumacult ALI basal media from HNAE cells basolateral side (Stemcell technologies). HAE cells were fully differentiated and ready to use after 28 days post-airlift ...
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bioRxiv - Systems Biology 2022Quote: ... and total peripheral blood mononuclear cells (PBMCs) were isolated by density centrifugation according to the manufacturers protocol (Lymphoprep, Stem Cell Technologies). Samples were cryopreserved at a concentration of 107 cells per milliliter in a solution of 90% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... total B cells from the spleens and lymph nodes were isolated using the Negative Selection B cell isolation kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed in IMDM+20%FCS and plated into 1.4 mL of cytokine-rich methylcellulose (Methocult H4435, Stem Cell Technologies). Colonies were scored 14 days later under an inverted microscope ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were dissociated into a single cell suspension with TrypLE and replated on matrigel-coated plates at a density of 40,000 cells/cm2 in mTesR medium (Stem Cell Technologies). Cells were allowed to propagate with a daily change of medium for 2 days ...
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bioRxiv - Microbiology 2021Quote: ... followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies) respectively ...
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bioRxiv - Microbiology 2021Quote: ... total CD4+ T cells were isolated from PBMCs by negative magnetic separation using EasySep Human CD4+ T cell isolation kit (StemCell Technology). Following treatment for 5 days (Fig ...
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bioRxiv - Developmental Biology 2022Quote: ... they were specified into a hiPSC-CM/stromal cell mixture using RPMI B27-containing 5 µM IWP-4 (Stem Cell Technologies) followed by another 7 days of RPMI B27+ (RPMI1640 GlutaMAX + 2% B27 supplement with insulin ...
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bioRxiv - Neuroscience 2022Quote: NPCs were passaged and plated at 50,000 cells/cm2 in STEMdiff™ Forebrain Neuron Differentiation Media (Stem Cell Tech cat#08600) on Corning® Matrigel® coated tissue culture treated plates and transduced with a NEUROG2 lentivirus (GeneCopoeia cat#LPP-T7381-Lv105-A00-S ...
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bioRxiv - Synthetic Biology 2020Quote: ... Regulatory T cells were isolated using the EasySep human CD4+CD127lowCD25+ regulatory T cell isolation kit according to manufacturer’s protocol (STEMCELL Technologies, 18063). Primary T cells were cultured in X-Vivo 15 media (Lonza ...
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bioRxiv - Neuroscience 2021Quote: ... were generated from confluent iPSC cultures by dissociating them to single cells and seeding them at a density of ∼4 × 106 cells per well of Aggrewell 800 6-well plates (StemCell Technologies), equating to ∼2200 cells per EB ...
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bioRxiv - Immunology 2020Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymoh nodes using CD19 Microbeads (Miltenyi) or Mouse B cell Isolation Kit (Stemcell Technologies). B cells were stimulated with LPS (10 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... at 37℃ for 10 min and then were seeded on to a vitronectin-coated six-well plate at 3×105 cell/well in mTeSR1 supplemented with 5μM ROCK inhibitor (Y-27632, Stem Cell Technology) for 24 h ...