Labshake search
Citations for Stemcell Technologies :
1101 - 1150 of 1346 citations for Human Anti NT5E Recombinant Antibody clone Hu101 28 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... NK cells were isolated from whole blood using a density based negative selection kit according to the manufacturer’s instructions (RosetteSep Human NK Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation ...
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bioRxiv - Bioengineering 2024Quote: ... CD56+CD3- NK cells were isolated from the PBMC population using the EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). NK cells were frozen at 2.5e6 or 5E6 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... Conditionned NSG mice were humanized with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
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bioRxiv - Microbiology 2022Quote: Memory CD4+ T cells were enriched by negative selection using the EasySep Human Memory CD4+ T Cell Enrichment Kit (STEMCELL Technologies #19157) following the recommended protocol ...
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bioRxiv - Bioengineering 2022Quote: ... via Ficoll-Paque (Cytavia) gradient selection for peripheral blood mono-nuclear cells followed by human CD8+ T cell negative magnetic selection (Stem Cell Technologies). Primary CD8+ T cells were thawed in supplemented RPMI 1640 2-12 hours prior to the start of experiments.
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Immunology 2023Quote: ... CD14+CD16-monocytes were isolated from peripheral blood mononuclear cells using immunomagnetic negative selection (EasySep Human Monocyte Isolation Kit, Stem Cell Technologies).
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bioRxiv - Immunology 2023Quote: ... Human CD4+ T cells were isolated from approximately 2 x 108 elutriated lymphocytes to approximately 90% purity by negative selection using RosetteSep human CD4+ T cell enrichment cocktail (15062; Stem Cell Technologies), using Ficoll/Hypaque (45-001-749 ...
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bioRxiv - Bioengineering 2024Quote: ... Neutrophils were retained in solution through negative immuno-magnetic separation through the EasySep™ Human Neutrophil Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Neutrophils were incubated in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2024Quote: Culture supernatants were prepared by growing 5×107 B cells purified from peripheral blood of either healthy donors or CLL patients by negative selection using RosetteSep™ Human B-cell enrichment Cocktail (#15064; STEMCELL Technologies), in 15 ml RPMI-1640 medium (#8758 ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and CD4+ T cells were isolated by immunomagnetic negative selection (EasySep™ Human CD4+ T Cell Enrichment Kit, StemCell Technologies). 250,000-500,000 CD4+ T cells per well were plated in 96-well V-bottom plates ...
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bioRxiv - Immunology 2024Quote: ... Remaining unstained cells was restimulated with 12.5 uL/mL Immunocult Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, catalog no. 10970). Forty-eight hours post-restimulation ...
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bioRxiv - Immunology 2024Quote: Primary monocytes were isolated via negative selection using the EasySep™ Human Monocyte Isolation Kit (Easy Sep, 1x10^9) (Stem Cell Technologies) according to the manufacturer’s protocols from PBMCs collected from healthy donors ...
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bioRxiv - Immunology 2024Quote: ... CD3+ Tresponder cells (previously enriched from an HLA-A3+ individual using the EasySep™ Human CD3 Positive Selection Kit II; STEMCELL Technologies) were labelled with CPD-eFluor™450 and added to the co-cultures at a 5:1 Tresponder:DC ratio ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were enriched from peripheral blood of HLA-A2− subjects using the Rosettesep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies) and CD25+ cells were separated using CD25 MicroBeads II (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: B cells were isolated from 2 healthy PBMC donors using the EasySep™ Human B-cell Isolation Kit (Catalog #17954) from StemCell Technologies. Cells were then stained with APC anti-IgD-APC ...
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bioRxiv - Immunology 2024Quote: ... cones obtained from the Oklahoma Blood Institute (Oklahoma City, OK) using negative selection with the EasySep™ Human T Cell Isolation Kit (cat. no. 17951; STEMCELL Technologies). Alternatively ...
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bioRxiv - Immunology 2024Quote: ... 10 x 106 PBMCs were enriched for B cells using CD3 T cell depletion via the EasySep™ Human CD3 Positive Selection Kit II (StemCell Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... the mice were irradiated (350 rads) and injected intravenously with CD3+ T cell-depleted cord blood (EasySep Human CD3 Positive Selection Kit, 18051, Stem Cell Technologies) containing approximately 105 human CD34+ hematopoietic stem cells (HSC ...
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bioRxiv - Immunology 2024Quote: ... B-cells were then purified from the other harvested PBMCs by negative selection using a Human B-cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Mario-CAR T cells were selected using the EasySep™ Human PE Positive Selection Kit II (Stem Cell Technologies, Cat-no. 17654) with PE NGFR antibody (Biolegend ...
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bioRxiv - Immunology 2024Quote: MDMi are created as described previously.80 Blood from healthy human donors is separated using the density gradient medium Lymphoprep (Stemcell technologies #07851) to isolate mononuclear cells ...
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bioRxiv - Neuroscience 2024Quote: ... B cells were then enriched by negative selection using EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (17963, Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Neural Precursor Cells (NSCs) were generated from human induced pluripotent stem cells (iPSCs) using STEMdiff™ SMADi Neural Induction kit (STEMCELL Technologies) via dual SMAD inhibition as per the manufacturers protocol ...
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bioRxiv - Cancer Biology 2024Quote: Primary human NK cells were isolated from PBMCs from three donors by negative selection using the EasySepTM Human NK Cell Isolation Kit (STEMCELL Technologies 17955), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSep™ Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies). Buffy coats were collected at the Siena University Hospital after obtaining written informed consent from each donor and processed following standard ethical procedures outlined in the Declaration of Helsinki ...
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bioRxiv - Immunology 2024Quote: PBMCs were thawed and CD4+ T cells were isolated by immunomagnetic negative selection (EasySep™ Human CD4+ T Cell Enrichment Kit, StemCell Technologies). 100,000-200,000 CD4+ T cells per well were plated in 96-well V-bottom plates and rested for 1.5 hours at 37 °C ...
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bioRxiv - Genetics 2024Quote: ... line Kolf2_c1 line (Wellcome Sanger Institute’s Human Induced Pluripotent Stem Cell Initiative) was cultured in TeSR-E8 complete culture media (Stem Cell Technologies #05991) (37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Effector natural killer (NK) cells were isolated from human peripheral blood using Rosette Sep NK isolation kit (Stem Cell Technologies, Vancouver, Canada). NK cells were stimulated overnight with IL-21 (20 ng/mL ...
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bioRxiv - Cell Biology 2024Quote: Fresh human intestinal samples were collected and kept in DMEM/F-12 with 15 mM HEPES (DMEM/F-12, STEMCELL Technologies, 36254) and 1% w/v Bovine Serum Albumin (BSA ...
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bioRxiv - Biochemistry 2024Quote: ... Monocytes were purified from the PBMC fraction using the EasySep™ Human CD14 Positive Selection Kit II (# 17858, STEMCELL Technologies, Cologne, Germany) according to the manufacturer’s instructions and seeded in 6-well cell culture plates (1.5 x 106 cells/well ...
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bioRxiv - Cancer Biology 2024Quote: ... BT-002-010) once a week and 7ul/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stem Cell Technologies, 10970) every other week ...
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bioRxiv - Developmental Biology 2024Quote: Apheresis cones were flushed using 10 mL of PBS +1% FBS + 2mM EDTA and incubated on a nutator at room temp for 20 min with 500 μL of RosetteSep Human NK Cell Enrichment Cocktail (Stem Cell Technologies). Following the incubation ...
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bioRxiv - Immunology 2024Quote: B cells from tonsils of 5 individuals were enriched using negative selection magnetic beads (EasySep Human B Cell Isolation kit, Stemcell technologies, #17954). They were then pooled to avoid batch effects ...
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bioRxiv - Immunology 2023Quote: ... Anti-CD3 biotin (STEMCELL Technologies) was also added at a concentration of 2.5 μL/mL to increase NK cell purity ...
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bioRxiv - Immunology 2024Quote: ... and anti-CD2 (Stemcell Technologies). Cells were cultured with atropine at 10 µM (Millipore Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... antibodies using EasySep Mouse Streptavidin RapidSpheres isolation kit (STEMCELL Technologies). Naïve CD4 T cells (0.1million ...
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bioRxiv - Biophysics 2022Quote: ... Human neutrophils were isolated from whole blood of healthy donors by immunomagnetic negative selection using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Isolation began from an initial blood volume of 0.5-1 ml ...
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bioRxiv - Bioengineering 2021Quote: Primary testicular cells were isolated from human testicular biopsies as previously described.[41] Testicular biopsies were transported to the lab on ice in Hypothermosol® FRS (STEMCELL Technologies, 07935) and processed within 1-2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
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bioRxiv - Bioengineering 2021Quote: ... the apical channel medium was removed from the human Lung Airway Chip and the basal medium was replaced with PneumaCult-ALI medium (PneumaCult™-ALI Medium, StemCell Technologies, 05001) supplemented with 1% penicillin-streptomycin ...
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bioRxiv - Bioengineering 2021Quote: ... After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971) for 3 days according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... were generated by positive selection of CD14+ cells from PBMCs using the EasySep Human CD14 Positive Selection Kit II (STEMCELL Technologies, Cat: 17858) and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... we first isolated CD4+ T cells from 10M PBMC by negative selection using the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies, Cat #: 19052). This yielded a mean of 1.6 (range 1.3-2.0 ...
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bioRxiv - Cancer Biology 2020Quote: Clonogenic assay was realized with cells seeded into 35mm petri dish in semi-solid methylcellulose medium (Methocult™ M3231 for murin cells or Methocult™ H4230 for human cells, Stemcell Technologies). Cells were treated with the drugs for 72 hrs and centrifugated ...
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bioRxiv - Cell Biology 2020Quote: Primary human airway epithelial cells isolated via bronchial brushings from consented healthy or asthmatic subjects were grown in PneumaCult ExPlus (Stemcell Technologies, Vancouver Canada) under submerged monolayer culture conditions and used in between passage 1 and 4 ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: Primary human CD8+ T cells were isolated from buffy coats of healthy donors and purified (>95%) by negative selection using the RosetteSep™ Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies, #15063), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
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bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...