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Citations for Stemcell Technologies :
1001 - 1050 of 1346 citations for Human Anti NT5E Recombinant Antibody clone Hu101 28 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Naïve B cells were then isolated from adult blood using the EasySep Human Memory B Cell Isolation kit (STEMCELL Technologies #17864) to first remove CD27 positive memory cells ...
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bioRxiv - Cell Biology 2024Quote: ... mature NK cells were isolated from peripheral blood mononucleated cells with the EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies). The human mesenchymal stromal cells (h-MSC ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ cells were seeded at the concentration of 5*105 CD34+ cells/ml in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
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bioRxiv - Cancer Biology 2024Quote: ... pups were irradiated with sublethal dose (1 Gy) and thereafter engrafted with 1×105 human CD34+ cord blood cells (HLA-A24:02, Stemcell Technologies) through intrahepatic injection ...
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bioRxiv - Cell Biology 2024Quote: The CD4+ T-cell subpopulation was isolated from the CD14- fraction of PBMCs using the Easysep human CD4+ T-cell isolation kit (Stemcell Technology) as per the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... 1mM EDTA buffer and monocytes were then isolated using the magnetic EasySep Human CD14+ Selection kit (STEMCELL Technologies, Vancouver, BC, Canada) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ HSPCs were obtained at >95% purity from pooled collections using a two-step Rosette-Sep/EasySep human CD34-positive selection kit (STEMCELL Technologies) according to the manufacturer’s protocols and/or FACS sorting ...
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bioRxiv - Immunology 2024Quote: ... T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada). Second- generation 4-1BB- costimulated TSHR CAR constructs were synthesized de novo (IDT ...
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bioRxiv - Immunology 2023Quote: ILC were isolated from PBMCs by negative selection using the EasySep™ Human Pan-ILC Enrichment Kit according to manufacturer’s instructions (Stemcell Technologies). ILC were sorted using FACSARIA™IIu (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were isolated from PBMCs using an EasySep Human CD4+ T Cell Isolation Kit (17952, StemCell Technologies, Canada) as per manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: ... the cell suspension resulting from the dissociation was enriched in CD45+ cells with an EasySep Release Human CD45 Positive Selection Kit (Stemcell Technologies) following the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2023Quote: ... ∼80% confluent human induced pluripotent stem cells (hPSCs) were dissociated into single cells with the Gentle Cell Dissociation Reagent (STEMCELL Technologies). Bioink consisting of 1.5mg/mL collagen with 5% Geltrex and high-density hPSCs (4×107 cells/mL ...
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bioRxiv - Immunology 2023Quote: ... B cell purification was performed according to manufacturer instructions with the Stem Cell EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies). After enrichment ...
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bioRxiv - Bioengineering 2024Quote: ... transduced cells were isolated with the EasySep Human CD271 Positive Selection Kit II with EasyEights™ EasySep™ Magnets (StemCell Technologies) according to the manufacturer protocol.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... human MM cells were cultured at a density of 1 × 104 cells in MethoCult™ H4230 media (Stem Cell Technologies, #04230) supplemented with 15% FBS and 10% Gibco Phytohemagglutinin M form (PHA-M ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng/ml) and PDGF-BB (10 ng/ml; Shenandoah) and heparin (2 ng/ml; Stem Cell Technologies).
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bioRxiv - Immunology 2022Quote: ... Bone marrow plasma cells (BMPC) were enriched from bone marrow mononuclear cells using the EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% DMSO in FBS.
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bioRxiv - Molecular Biology 2022Quote: ... Neutrophils were isolated from 20 ml of blood with EasySep™ Human Neutrophil Isolation Kit (STEMCELL Technologies, British Columbia, Canada, 19666) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The t(8;21)-positive patient primary cells were co-cultured on MSCs feeders and cultivated with SFEM II containing 1X Human Myeloid Expansion Supplement II (StemCell Technologies).
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Immunology 2022Quote: Monocytes isolated from BCs were obtained first by enrichment of the BC with 15µL of RosetteSep human monocyte enrichment cocktail (Stemcell Technologies, Sweden) per mL of BC ...
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bioRxiv - Immunology 2022Quote: Human CD3+ T cells were obtained from buffy coats of healthy donors by negative selection using RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies) and stored at -80ºC until use ...
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bioRxiv - Immunology 2022Quote: ... 12 day-expanded cells were further expanded for 7 days with ImmunoCult Human CD3/CD28/CD2 T Cell Activator (25 μl/ml; STEMCELL Technologies), then rested overnight with reduced IL-2 as above (total 20-day culture) ...
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bioRxiv - Microbiology 2022Quote: ... primary CD4+ T-cells were negatively enriched using EasySep Human CD4+ T-cell isolation kit (Catalog no. 17952; Stem Cell Technologies) and cultured in RPMI supplemented with 10% FBS and 20 U/ml of Interleukin-2 (IL-2 ...
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bioRxiv - Immunology 2023Quote: ... The percentage of CD3+ cells was determined by flow cytometry and T cells were stimulated with Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) per fabricator instructions in IMDM (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... The PBMC pellet was resuspended in PBS supplemented with 2% FBS and 1 mM EDTA and monocyte isolation was performed using the EasySepTM human monocyte isolation kit (Stemcell Technologies) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: Human CD8+ T cells were purified from buffy coats from healthy donors at the NIH Blood Bank using EasySepTM Human CD8+ T Cell Isolation Kit (StemCell Technologies). Approximately 2×104 CD8+ T cells were plated in each well of a 96-well plate immediately following isolation ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were isolated from PBMCs by CD14 positive selection using the EasySep™ Human CD14 Positive Selection Kit (18058; Stemcell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: Isolated B cells were plated in ImmunoCult-XF B Cell Base medium supplemented with Human B Cell Expansion Supplement (StemCell Technology), seeding at 2.5 x 105 cells/mL in an appropriate format to provide media at a depth of 2-5 mm ...
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bioRxiv - Bioengineering 2024Quote: ... CD4+ CD45RO+ memory T cells were then isolated by negative selection using an EasySepTM Human Memory CD4+ T Cell Enrichment Kit (STEMCELL Technologies).
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bioRxiv - Cancer Biology 2024Quote: NK cells were isolated from PBMCs of healthy donors by depleting CD3 cells using an EasySep human CD3 positive selection kit II (StemCell Technologies). The negative cell fraction was expanded using irradiated (100 Gy ...
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bioRxiv - Cancer Biology 2024Quote: Purified HSPCs were plated in 96 well plates containing megakaryocyte differentiation medium (StemSpan SFEM II with human low-density lipoproteins (STEMCELL Technologies) and Megakaryocyte Expansion Supplement (STEMCELL Technologies)) ...
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bioRxiv - Microbiology 2024Quote: ... CD14+ monocytes were then isolated from the PBMC fraction using the EasySep™ Human CD14 Positive Selection Kit II (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... In some experiments we also used an additional commercially available ENR medium (here denoted cENR; IntestiCult Human organoid differentiation medium; #100-0214, STEMCELL Technologies) with 100U/ml penicillin-streptomycin ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then stimulated with 12 μL/mL ImmunoCult™(TM) Human CD3/CD28 T Cell Activator (STEMCELL Technologies, cat. #10971).
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bioRxiv - Microbiology 2024Quote: ... We initiated the organoid development by plating hiPSCs at density 20- 30% in TeSR1 medium on a 6-well plate coated with human embryonic stem cell (hESC)-qualified basement membrane matrix (STEMCELL Technologies). The next day ...
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bioRxiv - Neuroscience 2024Quote: Human induced pluripotent stem cell (hiPSCs) were obtained from WiCell (IMR90-4/WISCi004, WiCell) and cultured in mTeSR+ medium (STEMCELL Technologies) supplemented with 1% (v/v ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were first enriched for memory B-cells using the EasySep Human Memory B Cell Isolation Kit (Stemcell Technologies, catalog #17864) and sequenced using the 10x Genomics pipeline as described by Hurtado et al.37 Sequencing data were analyzed using Cell Ranger and custom Python scripts.
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bioRxiv - Immunology 2024Quote: ... Blood monocytes and T cells were enriched and obtained by counterflow centrifugal elutriation using RosetteSep human monocyte and T cell enrichment cocktail (StemCell Technologies), respectively ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ T lymphocytes were isolated from PBMCs by positive selection using magnetic beads (EasySep Human CD4 positive selection kit II, StemCell Technologies) and activated using ImmunoCult human CD3/CD28 T cell activator cocktail (StemCell Technologies ...
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bioRxiv - Immunology 2024Quote: ... T cells were isolated from peripheral blood of healthy donors under a University of Wisconsin-Madison IRB-approved protocol (2017-1070) using the RosetteSep Human T Cell Enrichment Cocktail (cat. no. 15021, 15061; STEMCELL Technologies). The sequence for a third generation GD2-CD28-OX40-CD3ζ construct was generously provided by Malcolm Brenner (Baylor College of Medicine) ...
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bioRxiv - Immunology 2024Quote: ... γδ TCR+ cells were enriched by immunomagnetic negative selection using an EasySep Human Gamma/Delta T Cell Isolation Kit (STEMCELL Technologies). After purification ...
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bioRxiv - Immunology 2024Quote: ... at the final concentration of 0.3×106 cells/ml and stimulated with ImmunoCult™ Human CD2/CD3/CD28 T Cell Activator (Stemcell Technologies). In the next step ...
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bioRxiv - Immunology 2024Quote: ... CD3 negative selection was performed utilizing the EasySep™ Human APC Positive Selection Kit II (Stem Cell Technologies, Cat-no. 17661) with CD3 APC antibody (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Human CD8+ T cells were purified from healthy donor blood samples using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies). For cloning ...
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bioRxiv - Immunology 2024Quote: Monocytes were isolated from healthy human donor buffy coat (Department of Transfusion & Tissue Medicine of the Brno University Hospital) using RosetteSepTM Human Monocyte Enrichment Cocktail (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: When human iPSCs reached a confluency of approximately 95% they were passaged with AccutaseTM Cell Dissociation Reagent (Cat #07920, StemCell Technologies) and resuspended as single cells in mTesRTM1 medium with 10 µM Y-27632 (Tocris Bioscience) ...
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bioRxiv - Microbiology 2024Quote: CD4+ T-cells were isolated from frozen PBMCs from 5 study participants using the EasySep Human CD4+ T-Cell Enrichment Kit (Stemcell Technologies). 2 million CD4+ T-cells were then cultured in 96-well plates in duplicate in 200 μL R10+ media supplemented with 100 U/mL IL-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Human peripheral blood mononuclear cells from healthy donors were thawed from cryostorage in ImmunoCult-XF medium (Stem Cell Technologies # 100-0956) containing 100 U ml−1 IL-2 in the presence of ImmunoCult™ CD3/CD28/CD2 T cell activator (Stem Cell Technologies # 10970 ...
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bioRxiv - Bioengineering 2024Quote: Patient-derived glioblastoma stem cell lines SD2 and SD3 19 were transduced with GFP-expressing lentivirus or were genetically engineered with a doxycycline-inducible H2B-GFP reporter 19 and cultured in laminin-coated tissue culture flasks in complete NeuroCult NS-A proliferation medium for human cells (Stemcell Technologies). U87MG cells (ATCC ...